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The map label for this gene is rfbB [H]

Identifier: 222094944

GI number: 222094944

Start: 1274110

End: 1275078

Strand: Direct

Name: rfbB [H]

Synonym: BCQ_1282

Alternate gene names: 222094944

Gene position: 1274110-1275078 (Clockwise)

Preceding gene: 222094943

Following gene: 222094945

Centisome position: 24.44

GC content: 35.29

Gene sequence:

>969_bases
ATGAATATATTAGTAACAGGTGGGGCCGGATTTATTGGAAGTAATTTTGTGCATTATATGTTACAAAGCTATGAAACATA
TAAAATTATTAATTTTGATGCATTAACATATAGTGGAAACTTAAATAATGTAAAGTCTATTCAAGATCATCCGAATTACT
ATTTTGTAAAAGGTGAAATTCAAAATGGAGAGCTGCTGGAGCATGTTATTAAGGAACGTGATGTGCAAGTCATTGTCAAT
TTCGCAGCTGAATCACATGTGGACCGTAGTATTGAAAATCCGCTTCCTTTTTATGATACAAATGTAATTGGGACAGTCAC
CTTATTAGAATTAGTAAAAAAATATCCGCATATTAAACTCGTGCAAGTATCAACGGATGAGGTGTATGGCTCTTTAGGAA
AAACAGGGCGATTTACCGAGGAAACCCCGTTAGCTCCAAATAGTCCATATTCTTCAAGCAAAGCGAGCGCCGATATGATA
GCTTTATCTTATTATAAAACATATCACTTACCAGTTATAGTAACGCGTTGTTCCAATAACTACGGGCCGTATCAATATCC
TGAAAAATTAATTCCGTTAATGGTTACGAATGCGCTCGAAGGAAAAAAATTACCGTTATATGGCGATGGATTAAATGTAA
GAGATTGGTTACATGTAACGGATCATTGTCGTGCGATTGACGTTGTTTTGCATAAGGGACGTATAGGAGAAGTATATAAT
ATAGGTGGAAATAATGAAAAAACAAATATAGATGTTGTGGAACAAATTATTACTCTCTTAGGAAAAACGGAAAAGGATAT
TGAATATGTGACAGATCGTTTAGGACACGACCGCCGTTATGCGATTGATGCAGAGAAAATGAAGAATGAATTTGATTGGG
AACCGAAGTATACGTTTGAGCAAGGGTTACAAGAAACGGTGCAATGGTATGAGAAGAATGAGGAATGGTGGAAACCATTA
AAAAAGTAA

Upstream 100 bases:

>100_bases
AATTCCATGGCCGGTAACAAGCCCTATTTTGTCTGATAAAGATCGAATATTACCATCGCTTCAGGAATGTGAAGATAGTT
TTTGAGTAGGAGAGTTGTGT

Downstream 100 bases:

>100_bases
GGGGCATATAAATGAAAGAACGGATTATCATAACAGGAGCAAATGGTCAATTGGGAAAGCAACTACAAGAAGAGTTAAAT
CCTGAAGAATATGACATATA

Product: dtdp-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MNILVTGGAGFIGSNFVHYMLQSYETYKIINFDALTYSGNLNNVKSIQDHPNYYFVKGEIQNGELLEHVIKERDVQVIVN
FAAESHVDRSIENPLPFYDTNVIGTVTLLELVKKYPHIKLVQVSTDEVYGSLGKTGRFTEETPLAPNSPYSSSKASADMI
ALSYYKTYHLPVIVTRCSNNYGPYQYPEKLIPLMVTNALEGKKLPLYGDGLNVRDWLHVTDHCRAIDVVLHKGRIGEVYN
IGGNNEKTNIDVVEQIITLLGKTEKDIEYVTDRLGHDRRYAIDAEKMKNEFDWEPKYTFEQGLQETVQWYEKNEEWWKPL
KK

Sequences:

>Translated_322_residues
MNILVTGGAGFIGSNFVHYMLQSYETYKIINFDALTYSGNLNNVKSIQDHPNYYFVKGEIQNGELLEHVIKERDVQVIVN
FAAESHVDRSIENPLPFYDTNVIGTVTLLELVKKYPHIKLVQVSTDEVYGSLGKTGRFTEETPLAPNSPYSSSKASADMI
ALSYYKTYHLPVIVTRCSNNYGPYQYPEKLIPLMVTNALEGKKLPLYGDGLNVRDWLHVTDHCRAIDVVLHKGRIGEVYN
IGGNNEKTNIDVVEQIITLLGKTEKDIEYVTDRLGHDRRYAIDAEKMKNEFDWEPKYTFEQGLQETVQWYEKNEEWWKPL
KK
>Mature_322_residues
MNILVTGGAGFIGSNFVHYMLQSYETYKIINFDALTYSGNLNNVKSIQDHPNYYFVKGEIQNGELLEHVIKERDVQVIVN
FAAESHVDRSIENPLPFYDTNVIGTVTLLELVKKYPHIKLVQVSTDEVYGSLGKTGRFTEETPLAPNSPYSSSKASADMI
ALSYYKTYHLPVIVTRCSNNYGPYQYPEKLIPLMVTNALEGKKLPLYGDGLNVRDWLHVTDHCRAIDVVLHKGRIGEVYN
IGGNNEKTNIDVVEQIITLLGKTEKDIEYVTDRLGHDRRYAIDAEKMKNEFDWEPKYTFEQGLQETVQWYEKNEEWWKPL
KK

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=327, Percent_Identity=38.2262996941896, Blast_Score=243, Evalue=1e-64,
Organism=Homo sapiens, GI42516563, Length=322, Percent_Identity=27.3291925465839, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI4504031, Length=336, Percent_Identity=25.5952380952381, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI56237023, Length=340, Percent_Identity=25.5882352941176, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI56118217, Length=340, Percent_Identity=25.5882352941176, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI189083684, Length=340, Percent_Identity=25.5882352941176, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI48994969, Length=340, Percent_Identity=47.6470588235294, Blast_Score=324, Evalue=5e-90,
Organism=Escherichia coli, GI1788353, Length=341, Percent_Identity=46.6275659824047, Blast_Score=313, Evalue=6e-87,
Organism=Escherichia coli, GI1786974, Length=347, Percent_Identity=27.6657060518732, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788366, Length=233, Percent_Identity=27.0386266094421, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=322, Percent_Identity=38.8198757763975, Blast_Score=227, Evalue=8e-60,
Organism=Caenorhabditis elegans, GI115532424, Length=318, Percent_Identity=33.0188679245283, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17539532, Length=329, Percent_Identity=24.9240121580547, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71982038, Length=355, Percent_Identity=27.3239436619718, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71982035, Length=353, Percent_Identity=27.4787535410765, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319493, Length=347, Percent_Identity=27.0893371757925, Blast_Score=96, Evalue=8e-21,
Organism=Drosophila melanogaster, GI21356223, Length=326, Percent_Identity=27.9141104294479, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19923002, Length=344, Percent_Identity=28.7790697674419, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24158427, Length=331, Percent_Identity=24.4712990936556, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 36971; Mature: 36971

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILVTGGAGFIGSNFVHYMLQSYETYKIINFDALTYSGNLNNVKSIQDHPNYYFVKGEI
CEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCHHCCCCCEEEEEEEC
QNGELLEHVIKERDVQVIVNFAAESHVDRSIENPLPFYDTNVIGTVTLLELVKKYPHIKL
CCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
VQVSTDEVYGSLGKTGRFTEETPLAPNSPYSSSKASADMIALSYYKTYHLPVIVTRCSNN
EEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEHHHHCCCEEEEEEECCC
YGPYQYPEKLIPLMVTNALEGKKLPLYGDGLNVRDWLHVTDHCRAIDVVLHKGRIGEVYN
CCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHEEEEEHCCCCCCCEEE
IGGNNEKTNIDVVEQIITLLGKTEKDIEYVTDRLGHDRRYAIDAEKMKNEFDWEPKYTFE
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECHHHHHHCCCCCCCHHHH
QGLQETVQWYEKNEEWWKPLKK
HHHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure
MNILVTGGAGFIGSNFVHYMLQSYETYKIINFDALTYSGNLNNVKSIQDHPNYYFVKGEI
CEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCHHCCCCCEEEEEEEC
QNGELLEHVIKERDVQVIVNFAAESHVDRSIENPLPFYDTNVIGTVTLLELVKKYPHIKL
CCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
VQVSTDEVYGSLGKTGRFTEETPLAPNSPYSSSKASADMIALSYYKTYHLPVIVTRCSNN
EEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEHHHHCCCEEEEEEECCC
YGPYQYPEKLIPLMVTNALEGKKLPLYGDGLNVRDWLHVTDHCRAIDVVLHKGRIGEVYN
CCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHEEEEEHCCCCCCCEEE
IGGNNEKTNIDVVEQIITLLGKTEKDIEYVTDRLGHDRRYAIDAEKMKNEFDWEPKYTFE
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECHHHHHHCCCCCCCHHHH
QGLQETVQWYEKNEEWWKPLKK
HHHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]