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The map label for this gene is oppA [H]

Identifier: 222094908

GI number: 222094908

Start: 1239291

End: 1240922

Strand: Direct

Name: oppA [H]

Synonym: BCQ_1246

Alternate gene names: 222094908

Gene position: 1239291-1240922 (Clockwise)

Preceding gene: 222094905

Following gene: 222094909

Centisome position: 23.77

GC content: 35.17

Gene sequence:

>1632_bases
ATGAAGAAAAAGATACCGTTATTACTTGCATCGACGTTGACTGTCAGTATGTTAGGAGCTTGTAGTTACCAAAAAGAGGA
TAATAAAGCAGGCGCGAAAGAAAAATCCTCAAACAAGCAAGTGTTAAATCTAACTGAGACAGCTGAAATCCCAACTATGG
ATACGACGTTATCAACAGATGCAACATCTTCTAACATCATGAATAACACAATGGAAGGGTTATATCGTCTGGGGAAAGAT
GATAAACTTGTTCCGGGTGTCGCTAAATCTTATGAGAAATCAGAGGATGGCAAAAAGTATGTGTTTAAGTTACGTGAAGA
TGCGAAATGGTCTAATGGTGAACCTGTAACAGCGAAGGACTTCGTTTATTCTTGGCGAAGAGCTGTAGATTCAAACACGG
GTGCCAAGTTTGCTTACATACTATTTGATGTTAAAAATGCGGAGAAAGTTAACAAAAAAGAGTTACCAGTTGAAGAACTT
GGTGTAAAGGCAATTGATGATCATACACTTGAAGTGGAATTAGATAACCCTGTTCCTTATTTTGTAAGTTTAACAGTCTA
TCCAACATTGTATCCTTTGAATGAGAAGTTTGTAACAGAACAAGGAGCAAAATTTGGATTAGAATCCAATACGACACTTT
ATAATGGTCCATTCGTATTAAATGAATGGAAGCACGAACAAAGTTTCCAACTTAAGAAGAATCCAACGTATTGGGAAAAT
AAAGAAGTAAAACTTGAGGAAATAAACTTCAATATTGTTAAGGATCGTTCAACTGCTATAAATCTATATGAAACGAAAGC
AATTGATCGTGTAGTCTTAACATCAGAGTTTGTAGATAAATACAAATCAGATGCTGACTTTAAAACGATTAAGAGACCAT
CTACACAATTTATTCGCTTAAATGAAAAGAATAAATTTTTAGCAAATAAAAATATCCGAAAAGCAATTGCAATGTCCTTT
GAACGTGAAAACATCGGAAAAGTGATCTTAAACGATGGTTCAGAGGGTATATATGGTTTTGTTCCGAAAGGCTTAGCAAA
AGGTCCGAATGGAAAAGACTTCCGTGAAGAAAACGGAAAGCTTATAAAAGAGGATATGAAAGAAGCACAAAAATACTGGG
AAGCTGGTAAAAAAGAACTTGGTGTAGACAAAGTAGAGTTAGAGCTATTAAACTTTGATACTGATGATGCTAAAAAAATC
GGAGAGTATTTAAAAGGTCAATTTGAAAAGAACTTACCAGGTTTAACAGTTCCAACAAAAATGCAGCCATTTGCACAAAA
ATTAAAACTTGAAGCAAGTGGAGATTATGCAATGTCATATGCGGGATGGAGTCCAGATTATATGGATCCAATGTCATTCC
TTGAAATGTATACGACAGGTAATGCGCAAAACAAAGTACATTACGCAAACCCAGCGTATGATGATTTAATTAAAAAAGCA
AAAACAGAAGTTGATGTACAAGCTCGTTGGGATGCATTATTACAAGCGGAAAAACAACTGCTTGAAGATGCAGCAATTGC
TCCAGTATATCAACCAGGAAAAGCTTATTTACAACGTGGCTCAATTACTGGCCTATTAGAACATAAATATGGCGGAGAAT
TTAGCTATAAGTGGGTTGAACTAAAAAAATAA

Upstream 100 bases:

>100_bases
TATGTATAATGAAAATTGTAGAAACTTGGAGATTATTCTTTCAATTCTACTTTTTTACAAGTGAGGGTACAGGAAGTGCA
ATTAGGGGAGGCAACACAAC

Downstream 100 bases:

>100_bases
AAATGGTTTCCATCATGAATAAGGGTATATGCCTATGATGGGCATATGCTCTTATTTTAAAAAAATAAAAGTAAGAATAT
TTTAAACTATCTTATTGCTT

Product: oligopeptide ABC transporter substrate-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MKKKIPLLLASTLTVSMLGACSYQKEDNKAGAKEKSSNKQVLNLTETAEIPTMDTTLSTDATSSNIMNNTMEGLYRLGKD
DKLVPGVAKSYEKSEDGKKYVFKLREDAKWSNGEPVTAKDFVYSWRRAVDSNTGAKFAYILFDVKNAEKVNKKELPVEEL
GVKAIDDHTLEVELDNPVPYFVSLTVYPTLYPLNEKFVTEQGAKFGLESNTTLYNGPFVLNEWKHEQSFQLKKNPTYWEN
KEVKLEEINFNIVKDRSTAINLYETKAIDRVVLTSEFVDKYKSDADFKTIKRPSTQFIRLNEKNKFLANKNIRKAIAMSF
ERENIGKVILNDGSEGIYGFVPKGLAKGPNGKDFREENGKLIKEDMKEAQKYWEAGKKELGVDKVELELLNFDTDDAKKI
GEYLKGQFEKNLPGLTVPTKMQPFAQKLKLEASGDYAMSYAGWSPDYMDPMSFLEMYTTGNAQNKVHYANPAYDDLIKKA
KTEVDVQARWDALLQAEKQLLEDAAIAPVYQPGKAYLQRGSITGLLEHKYGGEFSYKWVELKK

Sequences:

>Translated_543_residues
MKKKIPLLLASTLTVSMLGACSYQKEDNKAGAKEKSSNKQVLNLTETAEIPTMDTTLSTDATSSNIMNNTMEGLYRLGKD
DKLVPGVAKSYEKSEDGKKYVFKLREDAKWSNGEPVTAKDFVYSWRRAVDSNTGAKFAYILFDVKNAEKVNKKELPVEEL
GVKAIDDHTLEVELDNPVPYFVSLTVYPTLYPLNEKFVTEQGAKFGLESNTTLYNGPFVLNEWKHEQSFQLKKNPTYWEN
KEVKLEEINFNIVKDRSTAINLYETKAIDRVVLTSEFVDKYKSDADFKTIKRPSTQFIRLNEKNKFLANKNIRKAIAMSF
ERENIGKVILNDGSEGIYGFVPKGLAKGPNGKDFREENGKLIKEDMKEAQKYWEAGKKELGVDKVELELLNFDTDDAKKI
GEYLKGQFEKNLPGLTVPTKMQPFAQKLKLEASGDYAMSYAGWSPDYMDPMSFLEMYTTGNAQNKVHYANPAYDDLIKKA
KTEVDVQARWDALLQAEKQLLEDAAIAPVYQPGKAYLQRGSITGLLEHKYGGEFSYKWVELKK
>Mature_543_residues
MKKKIPLLLASTLTVSMLGACSYQKEDNKAGAKEKSSNKQVLNLTETAEIPTMDTTLSTDATSSNIMNNTMEGLYRLGKD
DKLVPGVAKSYEKSEDGKKYVFKLREDAKWSNGEPVTAKDFVYSWRRAVDSNTGAKFAYILFDVKNAEKVNKKELPVEEL
GVKAIDDHTLEVELDNPVPYFVSLTVYPTLYPLNEKFVTEQGAKFGLESNTTLYNGPFVLNEWKHEQSFQLKKNPTYWEN
KEVKLEEINFNIVKDRSTAINLYETKAIDRVVLTSEFVDKYKSDADFKTIKRPSTQFIRLNEKNKFLANKNIRKAIAMSF
ERENIGKVILNDGSEGIYGFVPKGLAKGPNGKDFREENGKLIKEDMKEAQKYWEAGKKELGVDKVELELLNFDTDDAKKI
GEYLKGQFEKNLPGLTVPTKMQPFAQKLKLEASGDYAMSYAGWSPDYMDPMSFLEMYTTGNAQNKVHYANPAYDDLIKKA
KTEVDVQARWDALLQAEKQLLEDAAIAPVYQPGKAYLQRGSITGLLEHKYGGEFSYKWVELKK

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=532, Percent_Identity=29.6992481203008, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI87081878, Length=455, Percent_Identity=30.3296703296703, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1789397, Length=506, Percent_Identity=26.8774703557312, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1787762, Length=489, Percent_Identity=23.1083844580777, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1787551, Length=517, Percent_Identity=21.0831721470019, Blast_Score=86, Evalue=9e-18,
Organism=Escherichia coli, GI1787052, Length=365, Percent_Identity=23.5616438356164, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1789966, Length=511, Percent_Identity=20.9393346379648, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789887, Length=199, Percent_Identity=29.6482412060301, Blast_Score=73, Evalue=6e-14,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61626; Mature: 61626

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKIPLLLASTLTVSMLGACSYQKEDNKAGAKEKSSNKQVLNLTETAEIPTMDTTLSTD
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCC
ATSSNIMNNTMEGLYRLGKDDKLVPGVAKSYEKSEDGKKYVFKLREDAKWSNGEPVTAKD
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHH
FVYSWRRAVDSNTGAKFAYILFDVKNAEKVNKKELPVEELGVKAIDDHTLEVELDNPVPY
HHHHHHHHHCCCCCCEEEEEEEEECCCHHCCHHCCCHHHHCCEEECCCEEEEEECCCCCE
FVSLTVYPTLYPLNEKFVTEQGAKFGLESNTTLYNGPFVLNEWKHEQSFQLKKNPTYWEN
EEEEEEEEEEEECCHHHHHHCCCCCCCCCCCEEECCCEEEECCCCCCCEEECCCCCCCCC
KEVKLEEINFNIVKDRSTAINLYETKAIDRVVLTSEFVDKYKSDADFKTIKRPSTQFIRL
CCEEEEEECEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEE
NEKNKFLANKNIRKAIAMSFERENIGKVILNDGSEGIYGFVPKGLAKGPNGKDFREENGK
CCCCCCHHCCHHHHHHHHHHCCCCCCEEEECCCCCCEEEECCCHHCCCCCCCCHHHHCCC
LIKEDMKEAQKYWEAGKKELGVDKVELELLNFDTDDAKKIGEYLKGQFEKNLPGLTVPTK
CHHHHHHHHHHHHHCCHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
MQPFAQKLKLEASGDYAMSYAGWSPDYMDPMSFLEMYTTGNAQNKVHYANPAYDDLIKKA
CCHHHHHEEEECCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHHHH
KTEVDVQARWDALLQAEKQLLEDAAIAPVYQPGKAYLQRGSITGLLEHKYGGEFSYKWVE
HCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEEEEECCCCCEEEEEEE
LKK
ECC
>Mature Secondary Structure
MKKKIPLLLASTLTVSMLGACSYQKEDNKAGAKEKSSNKQVLNLTETAEIPTMDTTLSTD
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCC
ATSSNIMNNTMEGLYRLGKDDKLVPGVAKSYEKSEDGKKYVFKLREDAKWSNGEPVTAKD
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHH
FVYSWRRAVDSNTGAKFAYILFDVKNAEKVNKKELPVEELGVKAIDDHTLEVELDNPVPY
HHHHHHHHHCCCCCCEEEEEEEEECCCHHCCHHCCCHHHHCCEEECCCEEEEEECCCCCE
FVSLTVYPTLYPLNEKFVTEQGAKFGLESNTTLYNGPFVLNEWKHEQSFQLKKNPTYWEN
EEEEEEEEEEEECCHHHHHHCCCCCCCCCCCEEECCCEEEECCCCCCCEEECCCCCCCCC
KEVKLEEINFNIVKDRSTAINLYETKAIDRVVLTSEFVDKYKSDADFKTIKRPSTQFIRL
CCEEEEEECEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEE
NEKNKFLANKNIRKAIAMSFERENIGKVILNDGSEGIYGFVPKGLAKGPNGKDFREENGK
CCCCCCHHCCHHHHHHHHHHCCCCCCEEEECCCCCCEEEECCCHHCCCCCCCCHHHHCCC
LIKEDMKEAQKYWEAGKKELGVDKVELELLNFDTDDAKKIGEYLKGQFEKNLPGLTVPTK
CHHHHHHHHHHHHHCCHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
MQPFAQKLKLEASGDYAMSYAGWSPDYMDPMSFLEMYTTGNAQNKVHYANPAYDDLIKKA
CCHHHHHEEEECCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHHHH
KTEVDVQARWDALLQAEKQLLEDAAIAPVYQPGKAYLQRGSITGLLEHKYGGEFSYKWVE
HCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEEEEECCCCCEEEEEEE
LKK
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]