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The map label for this gene is nplT [H]

Identifier: 222094880

GI number: 222094880

Start: 1208532

End: 1209833

Strand: Direct

Name: nplT [H]

Synonym: BCQ_1218

Alternate gene names: 222094880

Gene position: 1208532-1209833 (Clockwise)

Preceding gene: 222094878

Following gene: 222094881

Centisome position: 23.18

GC content: 34.87

Gene sequence:

>1302_bases
ATGCGTGTGGGGAAAATACATACTAGGAAACTATTCATTTGTTTTTGTTTAGCTGTCGTTTTGTTTGTACCAATACATAC
ATTTGCAGACGAAAAAAGAGAGTGGCGAGACGAAGTTATATATTCCATTATGATTGATCGTTTTAATAATGGGGAACCGA
AAAATGACAAACAGTTAGAAGTTGGCAATTTAGAAGGATATCAGGGCGGAGATATAAGAGGTATTATAAAAAGACTGGAT
TACATAAAAAAAATGGGATTTACTACTGTTATGCTTTCGCCGCTTTTTGAAAGTGTAAAATACGATGGGTTAGACGTGCG
CAATTTTCAGAAGGTAAATGAACATTTCGGAACAGAAAATGATGTGAAAGAACTTTTGCAAGAAGCTCATGCAAAAGGAA
TGAAAGTTGTATTTCAATTTCCACTTGGTGAAAACGAACAGCAAGTAATCGACTCGATGAAATGGTGGCTCAAAGAAGTT
GATTTAGATGCAAGTTATGTAATACATAGTGAAAAAAAATCACCTGCTTTTTGGGATGATGTGCAAAAAGATATGCAAGT
GATAAAGAAAGATTTTCGAGTTATGACAAAAGAAGATAGTGAATACAACGAAAAAATAGTAGATGCGTTTTCCAAAGCGG
ACGTATCGGTGAAACCTTTATATGATGTAAGTAAAAAAGACGGAGCAGCCGCTACATTTTTAGATAATCAAGATACAAAA
AGATTTGCTCGTATTGCAAAAGAGAATATGAATTATCCACCATCTCGTTTGAAACTAGCTCTTACATATTTATTAACAAC
ACCAGGTATTCCGAATTTTTATTACGGAACTGAAATTGCATTGGATGGAGGAGGTACGCCAGATAATAGACGATTAATGG
ACTTCAAATCAGATGAAAAGTTTATGCAACATATAACGAAACTCGGTGAGCTTAGACAAGCGAGGCCATCTTTACGCCGC
GGTACGTTTGAGCTTCTATACGATAAAGATGGAATGAGTGTACTAAAGAGAAAGTATAAAGATGAAGTGACAGTAGTAGC
GATTAATAATACGAAAGAAACACAAAAAGTTGCTTTACCTGCAAGTGCGATTGGTGAAAAACAAGAGCTAAGGGGATTGT
TAGAAGATGAAATTATAAGAGAAGAAAACGGAAAATTTTATCTCGTTTTAAAGCGTGAGGAATCGAATGTGTATAAAGTT
AACGGGGAAACAGGTGTGAATTGGCTATTTATTTCTTTAATCGTCGGTGTGAACGTATTGTTTATTGCTTTTTTAATTGT
GGTTAAAAAGAGGCGGAAATAA

Upstream 100 bases:

>100_bases
TAGAAAACCTAACATGTATTAATTTGATAAGAATGAAATCAACGTATGCGATTCGTTTGGATAATTGGTATAATATTCTT
ACAATTAAAATACATGGGGG

Downstream 100 bases:

>100_bases
GGGGAGATTCTCTTTTATGTTCAATTAAAAGTGCGTGATGAGGTGAGATGTTTAAGTCTTCTTATCGTATTCGTAATTCT
GTATTTTGGGGATAACAGTG

Product: alpha-amylase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MRVGKIHTRKLFICFCLAVVLFVPIHTFADEKREWRDEVIYSIMIDRFNNGEPKNDKQLEVGNLEGYQGGDIRGIIKRLD
YIKKMGFTTVMLSPLFESVKYDGLDVRNFQKVNEHFGTENDVKELLQEAHAKGMKVVFQFPLGENEQQVIDSMKWWLKEV
DLDASYVIHSEKKSPAFWDDVQKDMQVIKKDFRVMTKEDSEYNEKIVDAFSKADVSVKPLYDVSKKDGAAATFLDNQDTK
RFARIAKENMNYPPSRLKLALTYLLTTPGIPNFYYGTEIALDGGGTPDNRRLMDFKSDEKFMQHITKLGELRQARPSLRR
GTFELLYDKDGMSVLKRKYKDEVTVVAINNTKETQKVALPASAIGEKQELRGLLEDEIIREENGKFYLVLKREESNVYKV
NGETGVNWLFISLIVGVNVLFIAFLIVVKKRRK

Sequences:

>Translated_433_residues
MRVGKIHTRKLFICFCLAVVLFVPIHTFADEKREWRDEVIYSIMIDRFNNGEPKNDKQLEVGNLEGYQGGDIRGIIKRLD
YIKKMGFTTVMLSPLFESVKYDGLDVRNFQKVNEHFGTENDVKELLQEAHAKGMKVVFQFPLGENEQQVIDSMKWWLKEV
DLDASYVIHSEKKSPAFWDDVQKDMQVIKKDFRVMTKEDSEYNEKIVDAFSKADVSVKPLYDVSKKDGAAATFLDNQDTK
RFARIAKENMNYPPSRLKLALTYLLTTPGIPNFYYGTEIALDGGGTPDNRRLMDFKSDEKFMQHITKLGELRQARPSLRR
GTFELLYDKDGMSVLKRKYKDEVTVVAINNTKETQKVALPASAIGEKQELRGLLEDEIIREENGKFYLVLKREESNVYKV
NGETGVNWLFISLIVGVNVLFIAFLIVVKKRRK
>Mature_433_residues
MRVGKIHTRKLFICFCLAVVLFVPIHTFADEKREWRDEVIYSIMIDRFNNGEPKNDKQLEVGNLEGYQGGDIRGIIKRLD
YIKKMGFTTVMLSPLFESVKYDGLDVRNFQKVNEHFGTENDVKELLQEAHAKGMKVVFQFPLGENEQQVIDSMKWWLKEV
DLDASYVIHSEKKSPAFWDDVQKDMQVIKKDFRVMTKEDSEYNEKIVDAFSKADVSVKPLYDVSKKDGAAATFLDNQDTK
RFARIAKENMNYPPSRLKLALTYLLTTPGIPNFYYGTEIALDGGGTPDNRRLMDFKSDEKFMQHITKLGELRQARPSLRR
GTFELLYDKDGMSVLKRKYKDEVTVVAINNTKETQKVALPASAIGEKQELRGLLEDEIIREENGKFYLVLKREESNVYKV
NGETGVNWLFISLIVGVNVLFIAFLIVVKKRRK

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1786604, Length=154, Percent_Identity=33.1168831168831, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789995, Length=128, Percent_Identity=26.5625, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1790687, Length=105, Percent_Identity=30.4761904761905, Blast_Score=65, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6322245, Length=112, Percent_Identity=29.4642857142857, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24583745, Length=162, Percent_Identity=30.2469135802469, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 50004; Mature: 50004

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGKIHTRKLFICFCLAVVLFVPIHTFADEKREWRDEVIYSIMIDRFNNGEPKNDKQLE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
VGNLEGYQGGDIRGIIKRLDYIKKMGFTTVMLSPLFESVKYDGLDVRNFQKVNEHFGTEN
ECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHH
DVKELLQEAHAKGMKVVFQFPLGENEQQVIDSMKWWLKEVDLDASYVIHSEKKSPAFWDD
HHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHH
VQKDMQVIKKDFRVMTKEDSEYNEKIVDAFSKADVSVKPLYDVSKKDGAAATFLDNQDTK
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCHHH
RFARIAKENMNYPPSRLKLALTYLLTTPGIPNFYYGTEIALDGGGTPDNRRLMDFKSDEK
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEECCCCCHHH
FMQHITKLGELRQARPSLRRGTFELLYDKDGMSVLKRKYKDEVTVVAINNTKETQKVALP
HHHHHHHHHHHHHHCHHHHCCCEEEEECCCCHHHHHHHHCCCEEEEEECCCCCHHHEECC
ASAIGEKQELRGLLEDEIIREENGKFYLVLKREESNVYKVNGETGVNWLFISLIVGVNVL
HHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHH
FIAFLIVVKKRRK
HHHHHHHHHHCCC
>Mature Secondary Structure
MRVGKIHTRKLFICFCLAVVLFVPIHTFADEKREWRDEVIYSIMIDRFNNGEPKNDKQLE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
VGNLEGYQGGDIRGIIKRLDYIKKMGFTTVMLSPLFESVKYDGLDVRNFQKVNEHFGTEN
ECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHH
DVKELLQEAHAKGMKVVFQFPLGENEQQVIDSMKWWLKEVDLDASYVIHSEKKSPAFWDD
HHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHH
VQKDMQVIKKDFRVMTKEDSEYNEKIVDAFSKADVSVKPLYDVSKKDGAAATFLDNQDTK
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCHHH
RFARIAKENMNYPPSRLKLALTYLLTTPGIPNFYYGTEIALDGGGTPDNRRLMDFKSDEK
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEECCCCCHHH
FMQHITKLGELRQARPSLRRGTFELLYDKDGMSVLKRKYKDEVTVVAINNTKETQKVALP
HHHHHHHHHHHHHHCHHHHCCCEEEEECCCCHHHHHHHHCCCEEEEEECCCCCHHHEECC
ASAIGEKQELRGLLEDEIIREENGKFYLVLKREESNVYKVNGETGVNWLFISLIVGVNVL
HHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHH
FIAFLIVVKKRRK
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]