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The map label for this gene is asnO [H]

Identifier: 222094876

GI number: 222094876

Start: 1202358

End: 1204136

Strand: Direct

Name: asnO [H]

Synonym: BCQ_1214

Alternate gene names: 222094876

Gene position: 1202358-1204136 (Clockwise)

Preceding gene: 222094874

Following gene: 222094877

Centisome position: 23.06

GC content: 38.22

Gene sequence:

>1779_bases
ATGGCTGAGACGTTAGCGAAGCGTGGCCCAGATGATAATAAAGTTTGGATTAAAGGCAATGTCGCATTTGGGCATAAACG
GTTAATCGTTGTTGATCCTGAAGGTGGTAAGCAGCCGATGACTTGCTTAAAGGACGAAGCGAATTATGCAATTTGCTATA
ACGGCGAACTGTATAACACGGAAGATATTCGAAAGGAACTATTAAGAAGAGGATATACGTTCAAAGGTCATTCTGATACG
GAAGTACTATTAGCATCGTATATGGAATGGAAAGAAGAATGTGTCGATCATTTAAACGGTATATATGCGTTTGCTGTATG
GGATGAACAGAAAGAACAAGTGTTTATTGCGCGCGATCGATTAGGGGTAAAACCGCTATTTTATAAATATGACAGTGGGC
GATTACTATTTGGCTCAGAGTTAAAAGCGATATTGGCGCATCCAGATGTGAAGGCGGAAGTGACGTTAGAAGGATTATCT
GAAATATTTGGCCTTGGACCATCTAGAACGCCTGGCCACGGCATTTATGCTGGTATAAAAGAATTACGTCCAGGTCATGC
GATGACATTTTCAAAGAACGGTTTATGCATATGGAGATATTGGAATGTAGAAAGTAAAAAGCATGAAGATTCCTTTGAAG
AAACAGTAGAAAAAACACGCTTTTTATTACAAGATGCGATTACAAGGCAGCTCGTTTCTGATGTACCATTATGTACTTTT
TTATCAGGCGGTGTAGATTCAAGCGCTATAACAGCAATTGCTGCGAAAGAATACGAAAGATCAGGAAAAGGGCAATTACA
CACATATTCGATTGATTACGAAGATAATGACAAATACTTTAAAGCAAATGCGTTTCAGCCAAATTCAGATGCACCATTTA
TTCATTTGATGACTGAAACGTTTCAAACAATCCATCATCGCTGCGTCATTTCAAATGAACAATTAGCGCAGTATTTAACC
GAAGCAGTGCTCGTTCGCGATTTACCTGGTATGGCAGATATTGATTCGTCATTATTATGGTTTTGTCGAGAGATTAAACA
AGATTTTGTCGTCGGTCTATCGGGGGAATGTGCAGATGAAATATTTGGTGGTTATCCGTGGTTTTATAGAGAGGATGATT
TACAATCGAGTGCATTTCCGTGGATGCGCTCTACAGAAGCGCGTGAACAACTGCTAAAAAAAGAATGGAGAAATAAATTA
AACTTACAACAATATGTACAACAGCGCTATGAAGAGTCCATTCAAGAAGTTCCTATTTTAGAGGGAGAAAGCCCAATTGA
AGCAAAGAGACGACAATTATTTTATTTAAATATGGTATGGTTTATGACAACATTATTAGACAGAAAAGACCGTATGAGTA
TGGGTGCAAGCTTAGAAGTACGCGTTCCATTTGCGGATCACCGACTTGTCGAATATGCTTGGAATATTCCTTGGGAAATG
AAAATGTATAAAAATCGCGAAAAAGGTCTATTACGTAAAGCGTTAGAAGGATTACTTCCAAATGACATCTTATATAGAAA
GAAGAGCCCGTATCCGAAAACACATAATCCGCACTATACGAAAGCAGTAACAGTATGGCTACAAGATTTATTAACAGATA
AAGGATCGATTTTACACGAACTATTTGATAAAGAACAGCTGAGTGGATTAATTGAGTCAGGCGGCAGTGCATTCCAAACG
CCATGGTATGGTCAATTAATGACTGGGCCACAACTTTTAGCTCATTTAGCACAAATTCACGTTTGGTTTAAAGAATATGG
AGTAAATATTAAAGAATAG

Upstream 100 bases:

>100_bases
AGAAAAGAAATAAGATGGAAGGATGATTTATATGTGCGGGATTACAGGATGGGTGGATTATAAACGCTCATTAGAAGGAG
AACGGGATGTCGTTACGAAG

Downstream 100 bases:

>100_bases
GAGAAAAGCGACTTACTTTATAAGTAAGTCGCTTTTTATTAAATAAATTGTCATATAAAATTCAAAATCATATTAGAAAA
AATACTTATTTAGAATAATT

Product: asparagine synthetase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 592; Mature: 591

Protein sequence:

>592_residues
MAETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGKQPMTCLKDEANYAICYNGELYNTEDIRKELLRRGYTFKGHSDT
EVLLASYMEWKEECVDHLNGIYAFAVWDEQKEQVFIARDRLGVKPLFYKYDSGRLLFGSELKAILAHPDVKAEVTLEGLS
EIFGLGPSRTPGHGIYAGIKELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVEKTRFLLQDAITRQLVSDVPLCTF
LSGGVDSSAITAIAAKEYERSGKGQLHTYSIDYEDNDKYFKANAFQPNSDAPFIHLMTETFQTIHHRCVISNEQLAQYLT
EAVLVRDLPGMADIDSSLLWFCREIKQDFVVGLSGECADEIFGGYPWFYREDDLQSSAFPWMRSTEAREQLLKKEWRNKL
NLQQYVQQRYEESIQEVPILEGESPIEAKRRQLFYLNMVWFMTTLLDRKDRMSMGASLEVRVPFADHRLVEYAWNIPWEM
KMYKNREKGLLRKALEGLLPNDILYRKKSPYPKTHNPHYTKAVTVWLQDLLTDKGSILHELFDKEQLSGLIESGGSAFQT
PWYGQLMTGPQLLAHLAQIHVWFKEYGVNIKE

Sequences:

>Translated_592_residues
MAETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGKQPMTCLKDEANYAICYNGELYNTEDIRKELLRRGYTFKGHSDT
EVLLASYMEWKEECVDHLNGIYAFAVWDEQKEQVFIARDRLGVKPLFYKYDSGRLLFGSELKAILAHPDVKAEVTLEGLS
EIFGLGPSRTPGHGIYAGIKELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVEKTRFLLQDAITRQLVSDVPLCTF
LSGGVDSSAITAIAAKEYERSGKGQLHTYSIDYEDNDKYFKANAFQPNSDAPFIHLMTETFQTIHHRCVISNEQLAQYLT
EAVLVRDLPGMADIDSSLLWFCREIKQDFVVGLSGECADEIFGGYPWFYREDDLQSSAFPWMRSTEAREQLLKKEWRNKL
NLQQYVQQRYEESIQEVPILEGESPIEAKRRQLFYLNMVWFMTTLLDRKDRMSMGASLEVRVPFADHRLVEYAWNIPWEM
KMYKNREKGLLRKALEGLLPNDILYRKKSPYPKTHNPHYTKAVTVWLQDLLTDKGSILHELFDKEQLSGLIESGGSAFQT
PWYGQLMTGPQLLAHLAQIHVWFKEYGVNIKE
>Mature_591_residues
AETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGKQPMTCLKDEANYAICYNGELYNTEDIRKELLRRGYTFKGHSDTE
VLLASYMEWKEECVDHLNGIYAFAVWDEQKEQVFIARDRLGVKPLFYKYDSGRLLFGSELKAILAHPDVKAEVTLEGLSE
IFGLGPSRTPGHGIYAGIKELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVEKTRFLLQDAITRQLVSDVPLCTFL
SGGVDSSAITAIAAKEYERSGKGQLHTYSIDYEDNDKYFKANAFQPNSDAPFIHLMTETFQTIHHRCVISNEQLAQYLTE
AVLVRDLPGMADIDSSLLWFCREIKQDFVVGLSGECADEIFGGYPWFYREDDLQSSAFPWMRSTEAREQLLKKEWRNKLN
LQQYVQQRYEESIQEVPILEGESPIEAKRRQLFYLNMVWFMTTLLDRKDRMSMGASLEVRVPFADHRLVEYAWNIPWEMK
MYKNREKGLLRKALEGLLPNDILYRKKSPYPKTHNPHYTKAVTVWLQDLLTDKGSILHELFDKEQLSGLIESGGSAFQTP
WYGQLMTGPQLLAHLAQIHVWFKEYGVNIKE

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=528, Percent_Identity=26.7045454545455, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI168229252, Length=528, Percent_Identity=26.7045454545455, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI168229250, Length=528, Percent_Identity=26.7045454545455, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI296010848, Length=525, Percent_Identity=26.6666666666667, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI296010852, Length=470, Percent_Identity=24.2553191489362, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI296010850, Length=470, Percent_Identity=24.2553191489362, Blast_Score=105, Evalue=2e-22,
Organism=Escherichia coli, GI1786889, Length=534, Percent_Identity=25.8426966292135, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17560178, Length=603, Percent_Identity=25.2072968490879, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71993933, Length=551, Percent_Identity=25.4083484573503, Blast_Score=118, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25147557, Length=551, Percent_Identity=25.0453720508167, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6325403, Length=549, Percent_Identity=24.9544626593807, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6321563, Length=546, Percent_Identity=24.3589743589744, Blast_Score=126, Evalue=9e-30,
Organism=Drosophila melanogaster, GI45553209, Length=524, Percent_Identity=25.763358778626, Blast_Score=129, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 68359; Mature: 68227

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGKQPMTCLKDEANYAICYNGELYNT
CCCCHHHCCCCCCEEEEEEEEEECCEEEEEECCCCCCCCHHHHHCCCCEEEEECCCEECH
EDIRKELLRRGYTFKGHSDTEVLLASYMEWKEECVDHLNGIYAFAVWDEQKEQVFIARDR
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCC
LGVKPLFYKYDSGRLLFGSELKAILAHPDVKAEVTLEGLSEIFGLGPSRTPGHGIYAGIK
CCCCEEEEEECCCCEEECCHHHHHHCCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHH
ELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVEKTRFLLQDAITRQLVSDVPLCTF
HCCCCCEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LSGGVDSSAITAIAAKEYERSGKGQLHTYSIDYEDNDKYFKANAFQPNSDAPFIHLMTET
HCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCEEEHHHHH
FQTIHHRCVISNEQLAQYLTEAVLVRDLPGMADIDSSLLWFCREIKQDFVVGLSGECADE
HHHHHHHHEECHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEECCCCCHHHH
IFGGYPWFYREDDLQSSAFPWMRSTEAREQLLKKEWRNKLNLQQYVQQRYEESIQEVPIL
HHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
EGESPIEAKRRQLFYLNMVWFMTTLLDRKDRMSMGASLEVRVPFADHRLVEYAWNIPWEM
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCHHHHHHHCCCEEE
KMYKNREKGLLRKALEGLLPNDILYRKKSPYPKTHNPHYTKAVTVWLQDLLTDKGSILHE
HHHHCHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHH
LFDKEQLSGLIESGGSAFQTPWYGQLMTGPQLLAHLAQIHVWFKEYGVNIKE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGKQPMTCLKDEANYAICYNGELYNT
CCCHHHCCCCCCEEEEEEEEEECCEEEEEECCCCCCCCHHHHHCCCCEEEEECCCEECH
EDIRKELLRRGYTFKGHSDTEVLLASYMEWKEECVDHLNGIYAFAVWDEQKEQVFIARDR
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCC
LGVKPLFYKYDSGRLLFGSELKAILAHPDVKAEVTLEGLSEIFGLGPSRTPGHGIYAGIK
CCCCEEEEEECCCCEEECCHHHHHHCCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHH
ELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVEKTRFLLQDAITRQLVSDVPLCTF
HCCCCCEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LSGGVDSSAITAIAAKEYERSGKGQLHTYSIDYEDNDKYFKANAFQPNSDAPFIHLMTET
HCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCEEEHHHHH
FQTIHHRCVISNEQLAQYLTEAVLVRDLPGMADIDSSLLWFCREIKQDFVVGLSGECADE
HHHHHHHHEECHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEECCCCCHHHH
IFGGYPWFYREDDLQSSAFPWMRSTEAREQLLKKEWRNKLNLQQYVQQRYEESIQEVPIL
HHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
EGESPIEAKRRQLFYLNMVWFMTTLLDRKDRMSMGASLEVRVPFADHRLVEYAWNIPWEM
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCHHHHHHHCCCEEE
KMYKNREKGLLRKALEGLLPNDILYRKKSPYPKTHNPHYTKAVTVWLQDLLTDKGSILHE
HHHHCHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHH
LFDKEQLSGLIESGGSAFQTPWYGQLMTGPQLLAHLAQIHVWFKEYGVNIKE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]