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The map label for this gene is oppA [H]

Identifier: 222094655

GI number: 222094655

Start: 983798

End: 985387

Strand: Direct

Name: oppA [H]

Synonym: BCQ_0993

Alternate gene names: 222094655

Gene position: 983798-985387 (Clockwise)

Preceding gene: 222094653

Following gene: 222094656

Centisome position: 18.87

GC content: 35.53

Gene sequence:

>1590_bases
ATGAAACGAAAATTATTTGCATTACCATTTGTCCTAATACTACTTATTGCATTAGCGGCTTGTTCTGGAGGAAAAGATTC
TTCGAAGCAAGCGGGCACTAGTAAATCAGGGACTCCGAAAGATGGAGGAACATTAACGATTGGCGTTAGCGATAACCCAG
AGACGATGAATCCGCTTTATGCGAATGATCGTGTGTCGTTAACTGTGCAGCAAGCTTTATATGCACCGCTGTATCATATG
GAAGACGGTAAGAAGAAATTTGTTCTTGCTGAAAGCTTTACGCCTTCAGAGGATCAATTAACGTGGACATTGAAGTTAAA
AGATAATTTGAAATGGCATGATGGTAAGAAAATTACATCAGACGACATAGCATTTACATTCCAATCTATTTTAGATGAGA
AGCAAAATAGCTCAAGTCGTGAAAACTTTGTTTTTAAAGGAAAACCGCTTGAGGTGAAAAAGGTAGATGAGTTAACAACT
CAATTCGTTTTACCACAAGTAAGTGCATCTTTTGAAGGTGTGATGAATGATTTCTTCCCAATTCCGAAACATGTATTTGA
AGGGGAAGCAGATTTAGCGAAGAGTAAGAAAAACTTACAACCTGTAGGATCAGGTCCGTTTAAGTTTAAAGAGTATAAGT
CTGATGAGTACGTTGCATTAGATCGATTTGATGATTATGTAGGTGGTAAAGCGAAATTAGACTCTATCGTATATCGCGTT
GTAAAAGACCGTAATACAGCAAATGTTTCACTACAAAATGGTCAAATCAATATGAAGATGATTGAGCCGCAAGACTTTAA
GAAATTAGATAGCACTGGGAACTTCTCAATGGTGACATTCCCAGAAGGTAGATTATTCTACTTATCTTATAACATGAATA
CAGATCTGATGAAGAAAAAAGAAGTGCGCCAAGCAATTGCGCATGCATTAGATAAGAAAGAAATGATTAACTCAGCATTC
GTTTCTAGTCAATTTGCAGAACCGGCAAATTCAATTTTAACGCCAGACGCTATGTATTATGCAAAAGACATTAAAGATTA
TAAGTATGATAAAAAAGAAGCGAAAGATTTATTAGCGAAAGCTGGCGTGAAAGATAAAGAAAAAGTACGCGTCATGTATG
TAACGAATAATAAGATTATGGAAAGCTTAGCGCTTTATACACAACAAAAGTTAAAAGAAGTTGGCTTAGAAGTTGAACTA
AATGCATTAGATGCTAGTGCGGCAAGTGAAAAAGGCTTAGATAAAGAGAATAAAGAATATGACATTACATTTGGTGGTTA
CATAATGGGACCTGAGCCAGATTCATATAAGAGCTTATTCTTAAGTAATGCTGAATACAATTATGCACGATATAAAAACG
CTGATTTCGATAAGTTATGGGAAGAAGCTGCAGTTGAAACAGATAAAACGAAACGCGCAGAACTATACCATAAAATTCAA
GAGACAGCTAGAGAAGATTTACCTTATCTACCAATTGCGTATCCGAAAGCGGTTATTGCAGTAGATAAAAAGTTTGATGG
ATTAAAAGAAGCAAAAGCAATTCCTGTCACAATGTTTGAAGATTTATCTAAGATTTATGAAGTGAAATAA

Upstream 100 bases:

>100_bases
TGACTTTATATAGAGGTAGTGGTAATTTAAAACTTATAAGAAAACTCGGGATTAATTCCGAGTTTTCTTATGGGTATAAC
TAGTTTGAGGGGGCCGAAAG

Downstream 100 bases:

>100_bases
GAAACAAACAGTAAAGAAGCTGGTGGAAATCAGCTTCTTTAGCTTGAGGGGGGAAGTTTGTGTATAAAGTAATTGCGAAG
AGGCTTTTAAATGCAATTCC

Product: oligopeptide ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MKRKLFALPFVLILLIALAACSGGKDSSKQAGTSKSGTPKDGGTLTIGVSDNPETMNPLYANDRVSLTVQQALYAPLYHM
EDGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITSDDIAFTFQSILDEKQNSSSRENFVFKGKPLEVKKVDELTT
QFVLPQVSASFEGVMNDFFPIPKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRV
VKDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKKEVRQAIAHALDKKEMINSAF
VSSQFAEPANSILTPDAMYYAKDIKDYKYDKKEAKDLLAKAGVKDKEKVRVMYVTNNKIMESLALYTQQKLKEVGLEVEL
NALDASAASEKGLDKENKEYDITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQ
ETAREDLPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDLSKIYEVK

Sequences:

>Translated_529_residues
MKRKLFALPFVLILLIALAACSGGKDSSKQAGTSKSGTPKDGGTLTIGVSDNPETMNPLYANDRVSLTVQQALYAPLYHM
EDGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITSDDIAFTFQSILDEKQNSSSRENFVFKGKPLEVKKVDELTT
QFVLPQVSASFEGVMNDFFPIPKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRV
VKDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKKEVRQAIAHALDKKEMINSAF
VSSQFAEPANSILTPDAMYYAKDIKDYKYDKKEAKDLLAKAGVKDKEKVRVMYVTNNKIMESLALYTQQKLKEVGLEVEL
NALDASAASEKGLDKENKEYDITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQ
ETAREDLPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDLSKIYEVK
>Mature_529_residues
MKRKLFALPFVLILLIALAACSGGKDSSKQAGTSKSGTPKDGGTLTIGVSDNPETMNPLYANDRVSLTVQQALYAPLYHM
EDGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITSDDIAFTFQSILDEKQNSSSRENFVFKGKPLEVKKVDELTT
QFVLPQVSASFEGVMNDFFPIPKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRV
VKDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKKEVRQAIAHALDKKEMINSAF
VSSQFAEPANSILTPDAMYYAKDIKDYKYDKKEAKDLLAKAGVKDKEKVRVMYVTNNKIMESLALYTQQKLKEVGLEVEL
NALDASAASEKGLDKENKEYDITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQ
ETAREDLPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDLSKIYEVK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=478, Percent_Identity=26.3598326359833, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1789966, Length=493, Percent_Identity=27.1805273833671, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787762, Length=530, Percent_Identity=26.6037735849057, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1789887, Length=539, Percent_Identity=24.1187384044527, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1787551, Length=432, Percent_Identity=22.2222222222222, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789397, Length=488, Percent_Identity=21.5163934426229, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1787495, Length=442, Percent_Identity=24.2081447963801, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87081878, Length=473, Percent_Identity=21.9873150105708, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59778; Mature: 59778

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKLFALPFVLILLIALAACSGGKDSSKQAGTSKSGTPKDGGTLTIGVSDNPETMNPLY
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCC
ANDRVSLTVQQALYAPLYHMEDGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITS
CCCEEEEEEHHHHHCCHHCCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCC
DDIAFTFQSILDEKQNSSSRENFVFKGKPLEVKKVDELTTQFVLPQVSASFEGVMNDFFP
HHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IPKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRV
CCHHHHCCCHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEECHHHHCCCHHHHHHHHHHH
VKDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKK
HHCCCCCEEEEECCEEEEEEECCHHHHHHCCCCCEEEEEECCCEEEEEEECCCHHHHHHH
EVRQAIAHALDKKEMINSAFVSSQFAEPANSILTPDAMYYAKDIKDYKYDKKEAKDLLAK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
AGVKDKEKVRVMYVTNNKIMESLALYTQQKLKEVGLEVELNALDASAASEKGLDKENKEY
CCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHCCCCCCCCEE
DITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQ
EEEECCEEECCCCCCCHHHEECCCCCCHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
ETAREDLPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDLSKIYEVK
HHHHHCCCCCCCCCCCEEEEECCCCCCHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRKLFALPFVLILLIALAACSGGKDSSKQAGTSKSGTPKDGGTLTIGVSDNPETMNPLY
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCC
ANDRVSLTVQQALYAPLYHMEDGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITS
CCCEEEEEEHHHHHCCHHCCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCC
DDIAFTFQSILDEKQNSSSRENFVFKGKPLEVKKVDELTTQFVLPQVSASFEGVMNDFFP
HHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IPKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRV
CCHHHHCCCHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEECHHHHCCCHHHHHHHHHHH
VKDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKK
HHCCCCCEEEEECCEEEEEEECCHHHHHHCCCCCEEEEEECCCEEEEEEECCCHHHHHHH
EVRQAIAHALDKKEMINSAFVSSQFAEPANSILTPDAMYYAKDIKDYKYDKKEAKDLLAK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
AGVKDKEKVRVMYVTNNKIMESLALYTQQKLKEVGLEVELNALDASAASEKGLDKENKEY
CCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHCCCCCCCCEE
DITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQ
EEEECCEEECCCCCCCHHHEECCCCCCHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
ETAREDLPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDLSKIYEVK
HHHHHCCCCCCCCCCCEEEEECCCCCCHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]