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The map label for this gene is alsS [H]

Identifier: 222094618

GI number: 222094618

Start: 939242

End: 940939

Strand: Direct

Name: alsS [H]

Synonym: BCQ_0956

Alternate gene names: 222094618

Gene position: 939242-940939 (Clockwise)

Preceding gene: 222094615

Following gene: 222094619

Centisome position: 18.01

GC content: 40.64

Gene sequence:

>1698_bases
ATGGTTAGTTTGAGTACAGGTGTAAAAGCAAACGACGTGAAGACAAAAACAAAAGGAGCAGATCTTGTTGTTGATTGTTT
AATTAAACAAGGTGTTACACATGTTTTCGGTATTCCAGGAGCGAAGATTGACTCTGTATTTGATGTACTGCAAGAAAGAG
GACCAGAGTTAATTGTTTGTCGTCATGAACAAAATGCAGCATTTATGGCAGCTGCGATTGGTAGATTAACAGGAAAACCG
GGCGTATGTCTTGTAACTTCAGGACCAGGGACATCAAATTTAGCGACAGGTCTTGTTACTGCGAATGCGGAGAGTGATCC
CGTTGTTGCTTTAGCTGGTGCAGTTCCACGTACAGATCGATTAAAACGTACGCACCAATCTATGGATAATGCTGCACTAT
TCGAACCAATCACAAAATATAGCGTAGAAGTAGAGCATCCTGATAATGTGCCAGAAGCATTATCAAATGCATTCCGAAGT
GCGACTTCCACAAATCCAGGCGCTACTTTAGTAAGTCTTCCGCAAGACGTTATGACTGCGGAAACGAATGTAGAGTCTAT
CGGTGCGCTTTCTAAGCCACAGCTTGGAATCGCTCCCACACATGATATTACATACGTAGTAGAAAAAATAAAAGCAGCGA
AATTGCCGGTTATTTTACTCGGTATGAGAGCAAGCACAAATGAAGTGACGAAAGCTGTTCGTGAATTAATTGCGGATACA
GAACTTCCTGTCGTTGAAACATATCAAGCAGCTGGTGCGATTTCACGTGAATTAGAAGATCATTTCTTCGGCCGCGTTGG
CTTATTCCGTAACCAACCAGGTGATATTTTACTAGAAGAAGCGGACCTTGTTATTTCTATCGGTTATGACCCAATTGAGT
ATGATCCGAAATTCTGGAATAAACTTGGGGACAGAACGATTATTCATCTTGATGACCATCAAGCAGATATCGATCATGAT
TACCAACCAGAGCGTGAATTAATTGGTGATATTGCCTTAACAGTAAATAGTATCGCAGAAAAATTACCGAAACTTGTGTT
AAGTACGAAATCAGAAGCAGTATTAGAACGATTACGTGCGAAATTATCAGAACAAGCAGAAGTTCCAAATCGTCCTTCAG
AAGGTGTTACGCATCCACTTCAAGTGATTCGTACACTTCGTTCTTTAATTAGTGACGATACAACTGTTACATGTGACATC
GGTTCCCATTCTATTTGGATGGCGAGATGTTTCCGTTCTTATGAACCACGTAGATTATTATTTAGTAACGGTATGCAAAC
GTTAGGTGTTGCACTCCCTTGGGCAATCGCAGCTACTTTAGTAGAACCAGGTAAAAAAGTAGTTTCCGTATCAGGTGACG
GTGGTTTCTTATTTTCAGCGATGGAGTTAGAAACGGCGGTACGCTTAAATTCTCCAATCGTCCATCTCGTTTGGAGAGAC
GGCACATATGATATGGTTGCATTCCAACAAATGATGAAATACGGCAGAACATCAGCTACAGAGTTTGGGGATGTTGATCT
TGTGAAATATGCGGAAAGTTTCGGGGCGTTAGGTCTTCGTGTTAACACGCCTGATGAATTAGAAGGGGTATTAAAATCTG
CATTAGCAGCAGATGGTCCTGTCATTATTGATATTCCAATTGACTATCGTGACAACATTAAATTAAGCGAAAAATTATTA
CCAAACCAATTAAACTAA

Upstream 100 bases:

>100_bases
AAAATAATGCATTTGTAATATAATGATAAATAATACTTCACCACGTTAATTAACCAGGTGAAGTTTAAAATCTTTATTAC
ACAAATTATGAAACAAAGGA

Downstream 100 bases:

>100_bases
TGGAGGCAGATTTGAGATGACGGTTGCGCAATTAATGGATATTGATGCAAAAAGAACGAAAACGAATAACGAAGTATATC
AAACATCTACAATGCTTGCG

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MVSLSTGVKANDVKTKTKGADLVVDCLIKQGVTHVFGIPGAKIDSVFDVLQERGPELIVCRHEQNAAFMAAAIGRLTGKP
GVCLVTSGPGTSNLATGLVTANAESDPVVALAGAVPRTDRLKRTHQSMDNAALFEPITKYSVEVEHPDNVPEALSNAFRS
ATSTNPGATLVSLPQDVMTAETNVESIGALSKPQLGIAPTHDITYVVEKIKAAKLPVILLGMRASTNEVTKAVRELIADT
ELPVVETYQAAGAISRELEDHFFGRVGLFRNQPGDILLEEADLVISIGYDPIEYDPKFWNKLGDRTIIHLDDHQADIDHD
YQPERELIGDIALTVNSIAEKLPKLVLSTKSEAVLERLRAKLSEQAEVPNRPSEGVTHPLQVIRTLRSLISDDTTVTCDI
GSHSIWMARCFRSYEPRRLLFSNGMQTLGVALPWAIAATLVEPGKKVVSVSGDGGFLFSAMELETAVRLNSPIVHLVWRD
GTYDMVAFQQMMKYGRTSATEFGDVDLVKYAESFGALGLRVNTPDELEGVLKSALAADGPVIIDIPIDYRDNIKLSEKLL
PNQLN

Sequences:

>Translated_565_residues
MVSLSTGVKANDVKTKTKGADLVVDCLIKQGVTHVFGIPGAKIDSVFDVLQERGPELIVCRHEQNAAFMAAAIGRLTGKP
GVCLVTSGPGTSNLATGLVTANAESDPVVALAGAVPRTDRLKRTHQSMDNAALFEPITKYSVEVEHPDNVPEALSNAFRS
ATSTNPGATLVSLPQDVMTAETNVESIGALSKPQLGIAPTHDITYVVEKIKAAKLPVILLGMRASTNEVTKAVRELIADT
ELPVVETYQAAGAISRELEDHFFGRVGLFRNQPGDILLEEADLVISIGYDPIEYDPKFWNKLGDRTIIHLDDHQADIDHD
YQPERELIGDIALTVNSIAEKLPKLVLSTKSEAVLERLRAKLSEQAEVPNRPSEGVTHPLQVIRTLRSLISDDTTVTCDI
GSHSIWMARCFRSYEPRRLLFSNGMQTLGVALPWAIAATLVEPGKKVVSVSGDGGFLFSAMELETAVRLNSPIVHLVWRD
GTYDMVAFQQMMKYGRTSATEFGDVDLVKYAESFGALGLRVNTPDELEGVLKSALAADGPVIIDIPIDYRDNIKLSEKLL
PNQLN
>Mature_565_residues
MVSLSTGVKANDVKTKTKGADLVVDCLIKQGVTHVFGIPGAKIDSVFDVLQERGPELIVCRHEQNAAFMAAAIGRLTGKP
GVCLVTSGPGTSNLATGLVTANAESDPVVALAGAVPRTDRLKRTHQSMDNAALFEPITKYSVEVEHPDNVPEALSNAFRS
ATSTNPGATLVSLPQDVMTAETNVESIGALSKPQLGIAPTHDITYVVEKIKAAKLPVILLGMRASTNEVTKAVRELIADT
ELPVVETYQAAGAISRELEDHFFGRVGLFRNQPGDILLEEADLVISIGYDPIEYDPKFWNKLGDRTIIHLDDHQADIDHD
YQPERELIGDIALTVNSIAEKLPKLVLSTKSEAVLERLRAKLSEQAEVPNRPSEGVTHPLQVIRTLRSLISDDTTVTCDI
GSHSIWMARCFRSYEPRRLLFSNGMQTLGVALPWAIAATLVEPGKKVVSVSGDGGFLFSAMELETAVRLNSPIVHLVWRD
GTYDMVAFQQMMKYGRTSATEFGDVDLVKYAESFGALGLRVNTPDELEGVLKSALAADGPVIIDIPIDYRDNIKLSEKLL
PNQLN

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=565, Percent_Identity=26.9026548672566, Blast_Score=194, Evalue=3e-49,
Organism=Homo sapiens, GI21361361, Length=599, Percent_Identity=25.542570951586, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=29.4014084507042, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1790104, Length=549, Percent_Identity=29.5081967213115, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI1786717, Length=573, Percent_Identity=26.8760907504363, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1787096, Length=534, Percent_Identity=26.5917602996255, Blast_Score=168, Evalue=1e-42,
Organism=Escherichia coli, GI1788716, Length=551, Percent_Identity=27.2232304900181, Blast_Score=153, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17542570, Length=575, Percent_Identity=26.2608695652174, Blast_Score=151, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=556, Percent_Identity=27.5179856115108, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17531301, Length=556, Percent_Identity=27.5179856115108, Blast_Score=148, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6323755, Length=562, Percent_Identity=27.7580071174377, Blast_Score=201, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6320816, Length=477, Percent_Identity=28.5115303983228, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6323163, Length=550, Percent_Identity=23.0909090909091, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19922626, Length=557, Percent_Identity=26.2118491921005, Blast_Score=164, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61307; Mature: 61307

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSLSTGVKANDVKTKTKGADLVVDCLIKQGVTHVFGIPGAKIDSVFDVLQERGPELIVC
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHCCCCEEEE
RHEQNAAFMAAAIGRLTGKPGVCLVTSGPGTSNLATGLVTANAESDPVVALAGAVPRTDR
EECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEECCCCCCEEEEECCCCCHHH
LKRTHQSMDNAALFEPITKYSVEVEHPDNVPEALSNAFRSATSTNPGATLVSLPQDVMTA
HHHHHHHCCCHHHHCCHHHCEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCHHHHHH
ETNVESIGALSKPQLGIAPTHDITYVVEKIKAAKLPVILLGMRASTNEVTKAVRELIADT
HCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCC
ELPVVETYQAAGAISRELEDHFFGRVGLFRNQPGDILLEEADLVISIGYDPIEYDPKFWN
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCCCCCHHHHH
KLGDRTIIHLDDHQADIDHDYQPERELIGDIALTVNSIAEKLPKLVLSTKSEAVLERLRA
HCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KLSEQAEVPNRPSEGVTHPLQVIRTLRSLISDDTTVTCDIGSHSIWMARCFRSYEPRRLL
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCHHH
FSNGMQTLGVALPWAIAATLVEPGKKVVSVSGDGGFLFSAMELETAVRLNSPIVHLVWRD
HHCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECC
GTYDMVAFQQMMKYGRTSATEFGDVDLVKYAESFGALGLRVNTPDELEGVLKSALAADGP
CCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCC
VIIDIPIDYRDNIKLSEKLLPNQLN
EEEEEECCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MVSLSTGVKANDVKTKTKGADLVVDCLIKQGVTHVFGIPGAKIDSVFDVLQERGPELIVC
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHCCCCEEEE
RHEQNAAFMAAAIGRLTGKPGVCLVTSGPGTSNLATGLVTANAESDPVVALAGAVPRTDR
EECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEECCCCCCEEEEECCCCCHHH
LKRTHQSMDNAALFEPITKYSVEVEHPDNVPEALSNAFRSATSTNPGATLVSLPQDVMTA
HHHHHHHCCCHHHHCCHHHCEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCHHHHHH
ETNVESIGALSKPQLGIAPTHDITYVVEKIKAAKLPVILLGMRASTNEVTKAVRELIADT
HCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCC
ELPVVETYQAAGAISRELEDHFFGRVGLFRNQPGDILLEEADLVISIGYDPIEYDPKFWN
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCCCCCHHHHH
KLGDRTIIHLDDHQADIDHDYQPERELIGDIALTVNSIAEKLPKLVLSTKSEAVLERLRA
HCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KLSEQAEVPNRPSEGVTHPLQVIRTLRSLISDDTTVTCDIGSHSIWMARCFRSYEPRRLL
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCHHH
FSNGMQTLGVALPWAIAATLVEPGKKVVSVSGDGGFLFSAMELETAVRLNSPIVHLVWRD
HHCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECC
GTYDMVAFQQMMKYGRTSATEFGDVDLVKYAESFGALGLRVNTPDELEGVLKSALAADGP
CCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCC
VIIDIPIDYRDNIKLSEKLLPNQLN
EEEEEECCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]