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The map label for this gene is blT [H]

Identifier: 222094583

GI number: 222094583

Start: 898979

End: 900154

Strand: Direct

Name: blT [H]

Synonym: BCQ_0921

Alternate gene names: 222094583

Gene position: 898979-900154 (Clockwise)

Preceding gene: 222094582

Following gene: 222094585

Centisome position: 17.24

GC content: 37.33

Gene sequence:

>1176_bases
ATGGTACTGGTCATTCTTTTGAGTAATATATTTATCGCTTTTTTAGGGATTGGATTAATCATTCCGGTTATGCCGTCCTT
TATGAATGATATGGGTTTAACAGGTAAGACGATGGGATATCTCGTTGCAGTGTTTGCAATGGCTCAGCTTATTGCTTCAC
CTATTACAGGCCGGTGGGTCGACCTTTATGGTAGGAAGAAAATGATAATCATTGGATTATTTATTTTTGGTGTTTCAGAG
CTTCTGTTTGGATTAGGAACAGATGTATGGATGCTCTATGCAGCGAGGGTGTTAGGCGGAATTAGTGCTGCGTTTATTAT
GCCTGGTGTTACGGCATATGTTGCGGATATTACATCTATACAGGAACGTCCAAAGGCGATGGGATATTTATCTGCTGCTA
TTAGTACCGGCTTTATTATAGGACCTGGAATCGGCGGATTTATTGCAGAATACGGTATACGTGTGCCGTTTTTCGTTGCA
GCAGTAATTGCCTTTGTAGCATGTGTGATCTCGATCTTTATTTTAAAAGAACCGTTAACGAAAGAAGAGCTTGCAGAGAT
TTCTTCTAATACGAAAGAATCAAGCTTTATTGGTGATTTAAAGAAATCATTACATCCAATGTATGCAATCGCATTTATTA
TCGTATTTGTACTTGCGTTCGGTTTATCGGCATATGAAACGGTGTTCAGTCTGTTTTCTGATCATAAGTTTGGATTCACA
CCGAAAGATATTGCGGCAATTATTACAATTAGTTCAATCTTTGGGGTAGTTGTGCAAGTATTTATGTTTGGAAAATTAGT
TGATATATTCGGTGAAAAAGTGTTAATTCAAATCTGTTTAATTGTAGGTGCAGTATTAGCGTTCGTTTCAACTGTAGTCT
TTAATTATTGGATTGTACTTCTCGTTACGTGCTTTATTTTCCTTGCATTTGATTTGCTTCGCCCAGCTTTAACGACGTTT
TTATCAAAAGCAGCTGGAAAAGAGCAAGGATTCGTTGCTGGTATGAACTCGACTTATACGAGTTTAGGAAATATCGCAGG
ACCAGCAATGGGCGGAATATTATTTGATATGAATATTCATTATCCATATGCATTTTCAGGAATTGTTTTAATAGTTGGTC
TCGCTATTACATTTATGTGGAGAGAAAAACAGCTAGCTGAAAGTTTTGCGAAGTAA

Upstream 100 bases:

>100_bases
AAATAGCAGGATAAAGAAAAAGCTTCTTGGGTATTATTTTTTTGAGGGGAGCTTACGGGAAGAGGTGTTGAAAGTGAAGA
AACCGATAAAAGAACAAAAG

Downstream 100 bases:

>100_bases
TCAAACCCCTTACAACGGTAAGGGGTTTTTGTTTTATAAACTTAATCCAAATCGTTTTAATTCAATAAAAATCCTCTCTA
ATTGCTTTCCTTTATCCGTT

Product: multidrug resistance protein b

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 2 [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MVLVILLSNIFIAFLGIGLIIPVMPSFMNDMGLTGKTMGYLVAVFAMAQLIASPITGRWVDLYGRKKMIIIGLFIFGVSE
LLFGLGTDVWMLYAARVLGGISAAFIMPGVTAYVADITSIQERPKAMGYLSAAISTGFIIGPGIGGFIAEYGIRVPFFVA
AVIAFVACVISIFILKEPLTKEELAEISSNTKESSFIGDLKKSLHPMYAIAFIIVFVLAFGLSAYETVFSLFSDHKFGFT
PKDIAAIITISSIFGVVVQVFMFGKLVDIFGEKVLIQICLIVGAVLAFVSTVVFNYWIVLLVTCFIFLAFDLLRPALTTF
LSKAAGKEQGFVAGMNSTYTSLGNIAGPAMGGILFDMNIHYPYAFSGIVLIVGLAITFMWREKQLAESFAK

Sequences:

>Translated_391_residues
MVLVILLSNIFIAFLGIGLIIPVMPSFMNDMGLTGKTMGYLVAVFAMAQLIASPITGRWVDLYGRKKMIIIGLFIFGVSE
LLFGLGTDVWMLYAARVLGGISAAFIMPGVTAYVADITSIQERPKAMGYLSAAISTGFIIGPGIGGFIAEYGIRVPFFVA
AVIAFVACVISIFILKEPLTKEELAEISSNTKESSFIGDLKKSLHPMYAIAFIIVFVLAFGLSAYETVFSLFSDHKFGFT
PKDIAAIITISSIFGVVVQVFMFGKLVDIFGEKVLIQICLIVGAVLAFVSTVVFNYWIVLLVTCFIFLAFDLLRPALTTF
LSKAAGKEQGFVAGMNSTYTSLGNIAGPAMGGILFDMNIHYPYAFSGIVLIVGLAITFMWREKQLAESFAK
>Mature_391_residues
MVLVILLSNIFIAFLGIGLIIPVMPSFMNDMGLTGKTMGYLVAVFAMAQLIASPITGRWVDLYGRKKMIIIGLFIFGVSE
LLFGLGTDVWMLYAARVLGGISAAFIMPGVTAYVADITSIQERPKAMGYLSAAISTGFIIGPGIGGFIAEYGIRVPFFVA
AVIAFVACVISIFILKEPLTKEELAEISSNTKESSFIGDLKKSLHPMYAIAFIIVFVLAFGLSAYETVFSLFSDHKFGFT
PKDIAAIITISSIFGVVVQVFMFGKLVDIFGEKVLIQICLIVGAVLAFVSTVVFNYWIVLLVTCFIFLAFDLLRPALTTF
LSKAAGKEQGFVAGMNSTYTSLGNIAGPAMGGILFDMNIHYPYAFSGIVLIVGLAITFMWREKQLAESFAK

Specific function: Energy-dependent efflux pump responsible for decreased drug accumulation in multi-drug-resistant cells. Probably uses a transmembrane proton gradient as the energy source. Causes the efflux of a variety of toxic substances, including such structurally div

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Homo sapiens, GI217416366, Length=422, Percent_Identity=21.3270142180095, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI166064062, Length=383, Percent_Identity=24.2819843342037, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI166064060, Length=383, Percent_Identity=24.2819843342037, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI39753965, Length=326, Percent_Identity=24.5398773006135, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1787291, Length=395, Percent_Identity=25.0632911392405, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081738, Length=349, Percent_Identity=22.6361031518625, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71996331, Length=382, Percent_Identity=24.0837696335079, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17551570, Length=163, Percent_Identity=31.2883435582822, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17738085, Length=350, Percent_Identity=24.8571428571429, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21357569, Length=372, Percent_Identity=25.8064516129032, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI221513297, Length=332, Percent_Identity=22.289156626506, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24656463, Length=372, Percent_Identity=25.8064516129032, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24656468, Length=372, Percent_Identity=25.8064516129032, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI161080926, Length=372, Percent_Identity=25.8064516129032, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI161080924, Length=372, Percent_Identity=25.8064516129032, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42327; Mature: 42327

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7608059; 8969508; 9384377 [H]