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The map label for this gene is malK [H]

Identifier: 222094328

GI number: 222094328

Start: 632322

End: 633923

Strand: Direct

Name: malK [H]

Synonym: BCQ_0642

Alternate gene names: 222094328

Gene position: 632322-633923 (Clockwise)

Preceding gene: 222094327

Following gene: 222094329

Centisome position: 12.13

GC content: 33.71

Gene sequence:

>1602_bases
ATGAAAAAAAGAAAAAGACTATGGAATTTATGGAAAACGATTACATTGTTAGTTTGTACTGTTGTAATTTTTTCTTTACT
TGTGACAGATATATTAATTAGTCATAATGTAGAACGGACAACGGAGGATAGCCAAGCAGAGAAAGCAAAAACAATTGCAC
ACATTGTGGCGAATGATTCGATTGTAATCGATGGTTTAATTGGGAAAGCGGATACTGCTGCAATCCAAACGTATACAAAT
AGAATATTAAAAAACACAGGTGTTCAATTTATTGTAGTTATGGACATGAATGGAATAAGAAAATCACATCCAAATCCTCA
AAAAATAGGTCATCATTTTATTGGGGGAGATGAAGGGCCTGCATTGAAAGGAAAGGAACATGTATCGCTGGCAGAAGGAA
CTTTAGGTATTTCAATGCGAGTGTTTGTACCTGTATTTTCTGAAACAGGTGAACAGCTTGGAGCGGTGGCCGTTGGTATT
TCAGCAGATAATGTAAAAGAGAGAGTTAAGGAAAGTAGACATATCATTTATATTGGTGTCGGAGTTGGAGTACTAGTCGG
AATTATAGGAGCAATATTGCTAGCTAGACATATAAAGAAAAGTTTATTCGGTCTTGAACCTCACAGGATAGCGAAAATTC
TTGAAGAAAGAAATACGATGTTACAATCTGTAAAGGAAGGTATTATTGCTGTAGATAAAGAGGCCAATGTAACTTTAATA
AATAATGAGGCAAAACGATTGTTTAAGAAAAGTGGATTAGAAGAAGATTTTATCGGTAAAGATGTTGAGTTATATATGCC
TAATTCACGTATAAAAGAAGTATTACAAACGGGAGAAGTACAATTAAACGAAGAACAAAATATTTATGGAATTACAATTG
TGACGAATCGTGTTCCTTTATATGTAAAAGGAGAAATAGTTGGCGCAATTGCAACATTTCGTGATAAAACAGAGATTAGA
AAATTAGCAGAAGAATTAACTGGTATTAGATTATATGCGGAAGCATTACGGGCACAATCGCATGAATTTATGAATAAAAT
GCATGTCGTATTAGGGCTTACACATATGAAGCAGTATGAAGAATTACAGAAATATATAAGCGGTATGGTATCAGAGCATC
AATATGAAATTGGTGGGATTATGAAAAGAATAAAAAGTCCAGTATTTGCTGGTTTTTTACTTGGGAAACTTAGCTATGCT
AGAGAGAAAAATATAAAATTAATTATAAGTGAAGATTCTTATATGCCAGAAATAGCGGATGAAAGTATTACTCATGAACT
AATTACGATTGTCGGAAATTTAATTGATAATGCATTAGAGGCAGTGACGAATTGTGAAAAGAAGCAAGTTGAAGTTGAGA
TACAATATGGGGATACACTAATCATTACAGTACAAGATACGGGAAAAGGTATACAAGAAGAAATAGGGGCATTATTTACG
AAAGGTTATTCCACAAAGGGGGATAACCGTGGTTATGGTTTGTATCTTGTAAAAGAAAGTATACAGCGAATAAATGGGGA
AATTCATGTGTATTCATTAGTAGGAAAGGGAACAACGATAACAATTGAAATACCTAAAGGTAGGGATGAGAGGCAAATAT
GA

Upstream 100 bases:

>100_bases
GACTAGTAAATTTAAAGTATAATAACAGCAGATAAGTAAGGTGAAAATAATATCACCTTACTTATTTTTCTATTCAGCAA
TTAGGGGAGTCATTAACGGG

Downstream 100 bases:

>100_bases
TTAAAGTTTTAATTGTAGAAGATGACCCGATGGTAGCGATGTTAAATACGCATTATTTAGAGCAAGTAGGAGGGTTTGAA
CTTGTTCAAGCAGTGAATTC

Product: sensory histidine kinase dcus

Products: NA

Alternate protein names: Malate kinase sensor [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MKKRKRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDSIVIDGLIGKADTAAIQTYTN
RILKNTGVQFIVVMDMNGIRKSHPNPQKIGHHFIGGDEGPALKGKEHVSLAEGTLGISMRVFVPVFSETGEQLGAVAVGI
SADNVKERVKESRHIIYIGVGVGVLVGIIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEANVTLI
NNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPLYVKGEIVGAIATFRDKTEIR
KLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYEELQKYISGMVSEHQYEIGGIMKRIKSPVFAGFLLGKLSYA
REKNIKLIISEDSYMPEIADESITHELITIVGNLIDNALEAVTNCEKKQVEVEIQYGDTLIITVQDTGKGIQEEIGALFT
KGYSTKGDNRGYGLYLVKESIQRINGEIHVYSLVGKGTTITIEIPKGRDERQI

Sequences:

>Translated_533_residues
MKKRKRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDSIVIDGLIGKADTAAIQTYTN
RILKNTGVQFIVVMDMNGIRKSHPNPQKIGHHFIGGDEGPALKGKEHVSLAEGTLGISMRVFVPVFSETGEQLGAVAVGI
SADNVKERVKESRHIIYIGVGVGVLVGIIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEANVTLI
NNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPLYVKGEIVGAIATFRDKTEIR
KLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYEELQKYISGMVSEHQYEIGGIMKRIKSPVFAGFLLGKLSYA
REKNIKLIISEDSYMPEIADESITHELITIVGNLIDNALEAVTNCEKKQVEVEIQYGDTLIITVQDTGKGIQEEIGALFT
KGYSTKGDNRGYGLYLVKESIQRINGEIHVYSLVGKGTTITIEIPKGRDERQI
>Mature_533_residues
MKKRKRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDSIVIDGLIGKADTAAIQTYTN
RILKNTGVQFIVVMDMNGIRKSHPNPQKIGHHFIGGDEGPALKGKEHVSLAEGTLGISMRVFVPVFSETGEQLGAVAVGI
SADNVKERVKESRHIIYIGVGVGVLVGIIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEANVTLI
NNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPLYVKGEIVGAIATFRDKTEIR
KLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYEELQKYISGMVSEHQYEIGGIMKRIKSPVFAGFLLGKLSYA
REKNIKLIISEDSYMPEIADESITHELITIVGNLIDNALEAVTNCEKKQVEVEIQYGDTLIITVQDTGKGIQEEIGALFT
KGYSTKGDNRGYGLYLVKESIQRINGEIHVYSLVGKGTTITIEIPKGRDERQI

Specific function: Member of a two-component regulatory system malK/malR. Involved in the activation of maeA, maeN and yflS in presence of malate. Probably activates malR by phosphorylation [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=526, Percent_Identity=40.4942965779468, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1786837, Length=527, Percent_Identity=30.3605313092979, Blast_Score=241, Evalue=8e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59338; Mature: 59338

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRKRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
IVIDGLIGKADTAAIQTYTNRILKNTGVQFIVVMDMNGIRKSHPNPQKIGHHFIGGDEGP
EEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHCCCCCCCCCCC
ALKGKEHVSLAEGTLGISMRVFVPVFSETGEQLGAVAVGISADNVKERVKESRHIIYIGV
CCCCCCCCEEECCCCCEEEEEEEEEHHHCCHHHCEEEEECCHHHHHHHHHHCCCEEEEEC
GVGVLVGIIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEANVTLI
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEE
NNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPL
CHHHHHHHHHCCCCHHHCCCCEEEECCCHHHHHHHHCCCEEECCCCCEEEEEEEECCCCE
YVKGEIVGAIATFRDKTEIRKLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYE
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELQKYISGMVSEHQYEIGGIMKRIKSPVFAGFLLGKLSYAREKNIKLIISEDSYMPEIAD
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHH
ESITHELITIVGNLIDNALEAVTNCEKKQVEVEIQYGDTLIITVQDTGKGIQEEIGALFT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEEECCCCCHHHHHHHHHH
KGYSTKGDNRGYGLYLVKESIQRINGEIHVYSLVGKGTTITIEIPKGRDERQI
CCCCCCCCCCCEEEEEEHHHHHHHCCCEEEEEEECCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MKKRKRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
IVIDGLIGKADTAAIQTYTNRILKNTGVQFIVVMDMNGIRKSHPNPQKIGHHFIGGDEGP
EEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHCCCCCCCCCCC
ALKGKEHVSLAEGTLGISMRVFVPVFSETGEQLGAVAVGISADNVKERVKESRHIIYIGV
CCCCCCCCEEECCCCCEEEEEEEEEHHHCCHHHCEEEEECCHHHHHHHHHHCCCEEEEEC
GVGVLVGIIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEANVTLI
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEE
NNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPL
CHHHHHHHHHCCCCHHHCCCCEEEECCCHHHHHHHHCCCEEECCCCCEEEEEEEECCCCE
YVKGEIVGAIATFRDKTEIRKLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYE
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELQKYISGMVSEHQYEIGGIMKRIKSPVFAGFLLGKLSYAREKNIKLIISEDSYMPEIAD
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHH
ESITHELITIVGNLIDNALEAVTNCEKKQVEVEIQYGDTLIITVQDTGKGIQEEIGALFT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEEECCCCCHHHHHHHHHH
KGYSTKGDNRGYGLYLVKESIQRINGEIHVYSLVGKGTTITIEIPKGRDERQI
CCCCCCCCCCCEEEEEEHHHHHHHCCCEEEEEEECCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 12949159; 12949160 [H]