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The map label for this gene is tnpR [H]

Identifier: 222094283

GI number: 222094283

Start: 577789

End: 578379

Strand: Reverse

Name: tnpR [H]

Synonym: BCQ_0596

Alternate gene names: 222094283

Gene position: 578379-577789 (Counterclockwise)

Preceding gene: 222094287

Following gene: 222094275

Centisome position: 11.09

GC content: 35.36

Gene sequence:

>591_bases
ATGATTTATGGTTACGCACGTGTAAGTACAAATAAGCAGGAACTGGACATTCAAATTAAAACATTAAAAGAAAAAGGTGC
TACTCAGATATATAAAGAAAAGATAAGTGGTGCTAAAGCTGATAGAAAAGAATTAAACAAGCTATTAGAAACAATAAAAG
CTGGTGACACACTTGTAGTAACTAAAGCTGACCGTATAGCACGTTCATTATCACAGCTAGAAGCATTCGTACTTGATTTA
ACTAATAGAGGTGTAGCGGTACATATAATCAATATGGGGCTTTTTACAGCGGAATCAATGGACAACCCTATAACAAAACT
ATTGTTTCAGGTATTAGGTTCATTTGCCGAGTTTGAACGTTCTATGATTCTCGAAAGAACACAAGAAGGTATAAAAGACG
CTAAAGAAAAAGGTGTTAAGTTTGGTAGGAAGTCTAAGAAATATGTGATTGAAGGTTCTTTATTACATGCTATTGAACAA
GTGGAAGAAGGGAAGATGTCACAGCCTGAAGGGGCTGAATTTGTTGGGTGTAGTGTAGCCACATTTAAAAGACGACTAAA
AGAATACAGAGAACAAAAAGGAAGTTCCTAG

Upstream 100 bases:

>100_bases
CTACACTTATTGACACTGATAAGAAACCCCTGTATTATAGGGTTTGTAAAGTATCAAAAAGTCGTATCAAATAAAAATTG
ATAAAAAGGGGAAATACATA

Downstream 100 bases:

>100_bases
TTCTATTTCATAACTGGGCTTTAAATTCCCTTCAGGAACTACAAGGGATAATAGCTGTGATTTTCAGCTAAACCAAACGG
TACAAAAAATGCCCACAAAC

Product: resolvase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MIYGYARVSTNKQELDIQIKTLKEKGATQIYKEKISGAKADRKELNKLLETIKAGDTLVVTKADRIARSLSQLEAFVLDL
TNRGVAVHIINMGLFTAESMDNPITKLLFQVLGSFAEFERSMILERTQEGIKDAKEKGVKFGRKSKKYVIEGSLLHAIEQ
VEEGKMSQPEGAEFVGCSVATFKRRLKEYREQKGSS

Sequences:

>Translated_196_residues
MIYGYARVSTNKQELDIQIKTLKEKGATQIYKEKISGAKADRKELNKLLETIKAGDTLVVTKADRIARSLSQLEAFVLDL
TNRGVAVHIINMGLFTAESMDNPITKLLFQVLGSFAEFERSMILERTQEGIKDAKEKGVKFGRKSKKYVIEGSLLHAIEQ
VEEGKMSQPEGAEFVGCSVATFKRRLKEYREQKGSS
>Mature_196_residues
MIYGYARVSTNKQELDIQIKTLKEKGATQIYKEKISGAKADRKELNKLLETIKAGDTLVVTKADRIARSLSQLEAFVLDL
TNRGVAVHIINMGLFTAESMDNPITKLLFQVLGSFAEFERSMILERTQEGIKDAKEKGVKFGRKSKKYVIEGSLLHAIEQ
VEEGKMSQPEGAEFVGCSVATFKRRLKEYREQKGSS

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=146, Percent_Identity=41.7808219178082, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787827, Length=150, Percent_Identity=36.6666666666667, Blast_Score=95, Evalue=2e-21,
Organism=Escherichia coli, GI1787638, Length=150, Percent_Identity=36.6666666666667, Blast_Score=95, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 21994; Mature: 21994

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS00397 RECOMBINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYGYARVSTNKQELDIQIKTLKEKGATQIYKEKISGAKADRKELNKLLETIKAGDTLVV
CEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEE
TKADRIARSLSQLEAFVLDLTNRGVAVHIINMGLFTAESMDNPITKLLFQVLGSFAEFER
ECHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHH
SMILERTQEGIKDAKEKGVKFGRKSKKYVIEGSLLHAIEQVEEGKMSQPEGAEFVGCSVA
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
TFKRRLKEYREQKGSS
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIYGYARVSTNKQELDIQIKTLKEKGATQIYKEKISGAKADRKELNKLLETIKAGDTLVV
CEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEE
TKADRIARSLSQLEAFVLDLTNRGVAVHIINMGLFTAESMDNPITKLLFQVLGSFAEFER
ECHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHH
SMILERTQEGIKDAKEKGVKFGRKSKKYVIEGSLLHAIEQVEEGKMSQPEGAEFVGCSVA
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
TFKRRLKEYREQKGSS
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]