Click here to switch to the map view.

The map label for this gene is alkA [H]

Identifier: 222094221

GI number: 222094221

Start: 512630

End: 513493

Strand: Direct

Name: alkA [H]

Synonym: BCQ_0514

Alternate gene names: 222094221

Gene position: 512630-513493 (Clockwise)

Preceding gene: 222094220

Following gene: 222094222

Centisome position: 9.83

GC content: 35.76

Gene sequence:

>864_bases
ATGTGGAGCGAACATGTTACGTTAGAGTATCCGTATCATTTTGAAGAAGTATTAAAACGTTTATCTTTTGATCCTCTTAA
CGTCATTCAATTAGATGAGAAAGTTATTTATGTCCCGCTTTGTATAGACGAGGAACAGATTGTTGTTCGCCTGCAAGGGA
TTGGTACTGTTCAAAATCCACAGTTTTGGATTTCTAGTCAGACAGGAGATCCAGAGAAAGTTATGAAACGAATGAGGGCC
ATTTTTCATTGGAATGAACCGTTTCAAGATATACAAAATCATTTTTTAAACACATCATTACGTCCACTATTTGAAACATA
TGCTTATACTCCAATTATTTTAGAATTTGATTATTTTGCTTGTCTTCTTCGCTGTATCATTCATCAACAAATAAATTTGA
AATTTGCTACTGTGTTAACAGAACAGTTTGTGAAACGATATGGAACAGAAAAGAACGGTGTATTCTTTTTCCCCACTCCA
GAAATAGTAGCAAATATTTCAATAGAGGAATTGAGAGAGCAGAAGTTTAGTCAGCGAAAGGCTGAATATATAGTAGGATT
AGGTCGAAGTATTGCCAGCGGTACATTAAATTTAGCGAGTATAGAAACCAAGGCGGAAGAAGAGATTTCAGCACAATTGT
TACCAATTAGGGGGATTGGTACATGGACAGTGCAAAATTTTTTAATGTTTGGGCTTGGACGGAAAAACATGTTTCCGAAA
GCGGACATTGGGATTCAGCGTGCAGTACAAGGTGTATTTCAATTAGATGATAAGCCTGATGATGCATTTTTAGAAAAAGT
GAAACAAGAGTGTGAACCATACTGCAGTTATGCAGCGTTATATTTATGGAAAAGTATAGAGTAG

Upstream 100 bases:

>100_bases
TTAAAAGTCTAGATGACTTAGTAAAAAGCAATCAACCGTAAGCATGTATGCTTGCGGTTTTCTCATGCTATAATGATGTG
TAAAAAGGATGGGGAAACGT

Downstream 100 bases:

>100_bases
AGGTGTAATAATGATACAAAAGCAACATGAGAGTAAGTTGGAAGTTGGTCAAACGTTCCCTGTGACAATTAAACGTCTTG
GGATTAACGGAGAAGGCGTT

Product: DNA-3-methyladenine glycosylase ii

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MWSEHVTLEYPYHFEEVLKRLSFDPLNVIQLDEKVIYVPLCIDEEQIVVRLQGIGTVQNPQFWISSQTGDPEKVMKRMRA
IFHWNEPFQDIQNHFLNTSLRPLFETYAYTPIILEFDYFACLLRCIIHQQINLKFATVLTEQFVKRYGTEKNGVFFFPTP
EIVANISIEELREQKFSQRKAEYIVGLGRSIASGTLNLASIETKAEEEISAQLLPIRGIGTWTVQNFLMFGLGRKNMFPK
ADIGIQRAVQGVFQLDDKPDDAFLEKVKQECEPYCSYAALYLWKSIE

Sequences:

>Translated_287_residues
MWSEHVTLEYPYHFEEVLKRLSFDPLNVIQLDEKVIYVPLCIDEEQIVVRLQGIGTVQNPQFWISSQTGDPEKVMKRMRA
IFHWNEPFQDIQNHFLNTSLRPLFETYAYTPIILEFDYFACLLRCIIHQQINLKFATVLTEQFVKRYGTEKNGVFFFPTP
EIVANISIEELREQKFSQRKAEYIVGLGRSIASGTLNLASIETKAEEEISAQLLPIRGIGTWTVQNFLMFGLGRKNMFPK
ADIGIQRAVQGVFQLDDKPDDAFLEKVKQECEPYCSYAALYLWKSIE
>Mature_287_residues
MWSEHVTLEYPYHFEEVLKRLSFDPLNVIQLDEKVIYVPLCIDEEQIVVRLQGIGTVQNPQFWISSQTGDPEKVMKRMRA
IFHWNEPFQDIQNHFLNTSLRPLFETYAYTPIILEFDYFACLLRCIIHQQINLKFATVLTEQFVKRYGTEKNGVFFFPTP
EIVANISIEELREQKFSQRKAEYIVGLGRSIASGTLNLASIETKAEEEISAQLLPIRGIGTWTVQNFLMFGLGRKNMFPK
ADIGIQRAVQGVFQLDDKPDDAFLEKVKQECEPYCSYAALYLWKSIE

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00730 HhH-GPD [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 33380; Mature: 33380

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWSEHVTLEYPYHFEEVLKRLSFDPLNVIQLDEKVIYVPLCIDEEQIVVRLQGIGTVQNP
CCCCCEEEECCCCHHHHHHHCCCCCCCEEEECCEEEEEEEEECCCEEEEEEECCCCCCCC
QFWISSQTGDPEKVMKRMRAIFHWNEPFQDIQNHFLNTSLRPLFETYAYTPIILEFDYFA
CEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHCCCEEEEEHHHHH
CLLRCIIHQQINLKFATVLTEQFVKRYGTEKNGVFFFPTPEIVANISIEELREQKFSQRK
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHCCCHHHHHHHHHHHHH
AEYIVGLGRSIASGTLNLASIETKAEEEISAQLLPIRGIGTWTVQNFLMFGLGRKNMFPK
HHHHHHCCCHHHCCCEEEEHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCCCCCCC
ADIGIQRAVQGVFQLDDKPDDAFLEKVKQECEPYCSYAALYLWKSIE
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MWSEHVTLEYPYHFEEVLKRLSFDPLNVIQLDEKVIYVPLCIDEEQIVVRLQGIGTVQNP
CCCCCEEEECCCCHHHHHHHCCCCCCCEEEECCEEEEEEEEECCCEEEEEEECCCCCCCC
QFWISSQTGDPEKVMKRMRAIFHWNEPFQDIQNHFLNTSLRPLFETYAYTPIILEFDYFA
CEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHCCCEEEEEHHHHH
CLLRCIIHQQINLKFATVLTEQFVKRYGTEKNGVFFFPTPEIVANISIEELREQKFSQRK
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHCCCHHHHHHHHHHHHH
AEYIVGLGRSIASGTLNLASIETKAEEEISAQLLPIRGIGTWTVQNFLMFGLGRKNMFPK
HHHHHHCCCHHHCCCEEEEHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCCCCCCC
ADIGIQRAVQGVFQLDDKPDDAFLEKVKQECEPYCSYAALYLWKSIE
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]