Click here to switch to the map view.

The map label for this gene is purF [H]

Identifier: 222094054

GI number: 222094054

Start: 322726

End: 324141

Strand: Direct

Name: purF [H]

Synonym: BCQ_0347

Alternate gene names: 222094054

Gene position: 322726-324141 (Clockwise)

Preceding gene: 222094053

Following gene: 222094055

Centisome position: 6.19

GC content: 40.11

Gene sequence:

>1416_bases
ATGCTTGCTGAAATAAAGGGGTTAAACGAAGAATGTGGCGTTTTCGGAATTTGGGGGCATGAAAATGCGGCACAAGTTTC
ATACTACGGATTGCACAGTTTACAGCACCGTGGACAAGAAGGCGCAGGCATTGTCGTAAATAATGGAGAAAAAATCGTCG
GTCACAAGGGGTTAGGTTTAATATCAGAAGTGTTTTCAAGAGGAGAGCTAGAAGGATTGAACGGAAAATCAGCAATCGGA
CACGTACGATACGCGACGGCTGGTGGAAGTGAAGTGGCTAACGTTCAACCATTACTATTCCGTTTTTCTGATCATAGTAT
GGCATTAGCTCATAACGGAAATTTAATTAACGCAAAAATGCTTCGTCGTGAATTAGAGGCAGAGGGAAGTATTTTTCAAA
CAAGTTCAGATACAGAAGTACTTTTACATCTTATTAAACGTAGTACGAAAGATTCGTTAATTGAAAGTGTAAAAGAAGCT
TTAAATAAAGTGAAAGGTGCGTTTGCATATCTTTTACTAACTGGAAATGAAATGATTGTTGCGCTAGATCCAAATGGATT
CCGTCCCCTTTCAATTGGAAAGATGGGAGATGCTTACGTTGTAGCATCAGAAACATGTGCTTTTGATGTAGTAGGTGCAA
CATACATTCGTGATGTAGAACCGGGTGAATTACTTATCATTAATGATGATGGAATTCACGTAGATCGTTTTACAAATGAT
GTAGAACATGCAATTTGCAGTATGGAATACATTTACTTCGCACGCCCAGATTCAAATATCGCTGGTGTTAATGTCCATGC
AGCACGTAAAAACATGGGGAAACGTTTGGCGGCAGAAGCTCCTATTGAAGCTGATGTTGTTACTGGTGTACCAGACTCTA
GCATTTCAGCAGCAATTGGTTATGCGGAGGCGACAGGCATTCCGTATGAGTTAGGATTAATTAAAAATCGTTACGTTGGA
CGTACGTTTATCCAACCTTCTCAAGAACTGCGCGAGCAAGGGGTTAAGATGAAACTTTCCGCAGTAAGAGGTGTGGTTGA
GGGGAAACGAGTTGTTATGATTGATGACTCTATCGTAAGAGGAACGACAAGTAAACGAATTGTTCGTATGCTTCGCGAGG
CTGGAGCGACAGAAGTTCACGTAAGAATTGCTTCACCGCCTTTGAAATATCCTTGTTTCTATGGCATTGATATTCAAACG
AGAAAAGAATTAATTGCAGCAAATCATACGGTAGAAGAAATTCGTGAAATGATCGGTGCAGACTCATTAACATTTTTAAG
TGAAGATGGATTAGTAGATGCAATTGGACGTCCATATGAAGGGAAATACGGTGGCCTTTGTATGGCGTACTTCAATGGAG
ATTATCCAACAGCTCTTTATGATTATGAGCAAGAGCTTTTAGAAAGTATGAAATAA

Upstream 100 bases:

>100_bases
TAACAAATACAAATGAAGTGACAATTCATAATGAAGAAAATGAAGTATTACTTACAGCAAATGTAGATGAAATGAGAAAG
GCTTGGAAAGGGGCAATCCC

Downstream 100 bases:

>100_bases
GAAGAAAAAAGCTTCTACTTCTTTTGATGTAGAAGCTAGCTTCTTTCTTAAAACGGCCCGCACAGGATAGGGAGAAATTA
AAAGACGAGGTGTAAATAAC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MLAEIKGLNEECGVFGIWGHENAAQVSYYGLHSLQHRGQEGAGIVVNNGEKIVGHKGLGLISEVFSRGELEGLNGKSAIG
HVRYATAGGSEVANVQPLLFRFSDHSMALAHNGNLINAKMLRRELEAEGSIFQTSSDTEVLLHLIKRSTKDSLIESVKEA
LNKVKGAFAYLLLTGNEMIVALDPNGFRPLSIGKMGDAYVVASETCAFDVVGATYIRDVEPGELLIINDDGIHVDRFTND
VEHAICSMEYIYFARPDSNIAGVNVHAARKNMGKRLAAEAPIEADVVTGVPDSSISAAIGYAEATGIPYELGLIKNRYVG
RTFIQPSQELREQGVKMKLSAVRGVVEGKRVVMIDDSIVRGTTSKRIVRMLREAGATEVHVRIASPPLKYPCFYGIDIQT
RKELIAANHTVEEIREMIGADSLTFLSEDGLVDAIGRPYEGKYGGLCMAYFNGDYPTALYDYEQELLESMK

Sequences:

>Translated_471_residues
MLAEIKGLNEECGVFGIWGHENAAQVSYYGLHSLQHRGQEGAGIVVNNGEKIVGHKGLGLISEVFSRGELEGLNGKSAIG
HVRYATAGGSEVANVQPLLFRFSDHSMALAHNGNLINAKMLRRELEAEGSIFQTSSDTEVLLHLIKRSTKDSLIESVKEA
LNKVKGAFAYLLLTGNEMIVALDPNGFRPLSIGKMGDAYVVASETCAFDVVGATYIRDVEPGELLIINDDGIHVDRFTND
VEHAICSMEYIYFARPDSNIAGVNVHAARKNMGKRLAAEAPIEADVVTGVPDSSISAAIGYAEATGIPYELGLIKNRYVG
RTFIQPSQELREQGVKMKLSAVRGVVEGKRVVMIDDSIVRGTTSKRIVRMLREAGATEVHVRIASPPLKYPCFYGIDIQT
RKELIAANHTVEEIREMIGADSLTFLSEDGLVDAIGRPYEGKYGGLCMAYFNGDYPTALYDYEQELLESMK
>Mature_471_residues
MLAEIKGLNEECGVFGIWGHENAAQVSYYGLHSLQHRGQEGAGIVVNNGEKIVGHKGLGLISEVFSRGELEGLNGKSAIG
HVRYATAGGSEVANVQPLLFRFSDHSMALAHNGNLINAKMLRRELEAEGSIFQTSSDTEVLLHLIKRSTKDSLIESVKEA
LNKVKGAFAYLLLTGNEMIVALDPNGFRPLSIGKMGDAYVVASETCAFDVVGATYIRDVEPGELLIINDDGIHVDRFTND
VEHAICSMEYIYFARPDSNIAGVNVHAARKNMGKRLAAEAPIEADVVTGVPDSSISAAIGYAEATGIPYELGLIKNRYVG
RTFIQPSQELREQGVKMKLSAVRGVVEGKRVVMIDDSIVRGTTSKRIVRMLREAGATEVHVRIASPPLKYPCFYGIDIQT
RKELIAANHTVEEIREMIGADSLTFLSEDGLVDAIGRPYEGKYGGLCMAYFNGDYPTALYDYEQELLESMK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=463, Percent_Identity=40.3887688984881, Blast_Score=345, Evalue=4e-95,
Organism=Escherichia coli, GI1788651, Length=475, Percent_Identity=39.7894736842105, Blast_Score=321, Evalue=6e-89,
Organism=Escherichia coli, GI1790167, Length=207, Percent_Identity=29.951690821256, Blast_Score=90, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17554892, Length=481, Percent_Identity=39.9168399168399, Blast_Score=339, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6323958, Length=493, Percent_Identity=36.105476673428, Blast_Score=273, Evalue=4e-74,
Organism=Drosophila melanogaster, GI28573187, Length=500, Percent_Identity=41.6, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24659598, Length=492, Percent_Identity=40.0406504065041, Blast_Score=358, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24659604, Length=463, Percent_Identity=40.8207343412527, Blast_Score=351, Evalue=6e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51377; Mature: 51377

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAEIKGLNEECGVFGIWGHENAAQVSYYGLHSLQHRGQEGAGIVVNNGEKIVGHKGLGL
CCCCCCCCCCCCCEEEEECCCCCCEEEEHHHHHHHHCCCCCCEEEEECCCEEECCCCCHH
ISEVFSRGELEGLNGKSAIGHVRYATAGGSEVANVQPLLFRFSDHSMALAHNGNLINAKM
HHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCEEEHHH
LRRELEAEGSIFQTSSDTEVLLHLIKRSTKDSLIESVKEALNKVKGAFAYLLLTGNEMIV
HHHHHHCCCCEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEEEEECCEEEE
ALDPNGFRPLSIGKMGDAYVVASETCAFDVVGATYIRDVEPGELLIINDDGIHVDRFTND
EECCCCCCEEEECCCCCEEEEEECCCEEEEECHHHEECCCCCCEEEECCCCEEEHHHHHH
VEHAICSMEYIYFARPDSNIAGVNVHAARKNMGKRLAAEAPIEADVVTGVPDSSISAAIG
HHHHHHCEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHCC
YAEATGIPYELGLIKNRYVGRTFIQPSQELREQGVKMKLSAVRGVVEGKRVVMIDDSIVR
HHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHCCCEEEEHHHHHHHCCCEEEEECCCHHC
GTTSKRIVRMLREAGATEVHVRIASPPLKYPCFYGIDIQTRKELIAANHTVEEIREMIGA
CCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHHCCCHHHHHHHHCC
DSLTFLSEDGLVDAIGRPYEGKYGGLCMAYFNGDYPTALYDYEQELLESMK
CCEEEEECCCCHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLAEIKGLNEECGVFGIWGHENAAQVSYYGLHSLQHRGQEGAGIVVNNGEKIVGHKGLGL
CCCCCCCCCCCCCEEEEECCCCCCEEEEHHHHHHHHCCCCCCEEEEECCCEEECCCCCHH
ISEVFSRGELEGLNGKSAIGHVRYATAGGSEVANVQPLLFRFSDHSMALAHNGNLINAKM
HHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCEEEHHH
LRRELEAEGSIFQTSSDTEVLLHLIKRSTKDSLIESVKEALNKVKGAFAYLLLTGNEMIV
HHHHHHCCCCEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEEEEECCEEEE
ALDPNGFRPLSIGKMGDAYVVASETCAFDVVGATYIRDVEPGELLIINDDGIHVDRFTND
EECCCCCCEEEECCCCCEEEEEECCCEEEEECHHHEECCCCCCEEEECCCCEEEHHHHHH
VEHAICSMEYIYFARPDSNIAGVNVHAARKNMGKRLAAEAPIEADVVTGVPDSSISAAIG
HHHHHHCEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHCC
YAEATGIPYELGLIKNRYVGRTFIQPSQELREQGVKMKLSAVRGVVEGKRVVMIDDSIVR
HHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHCCCEEEEHHHHHHHCCCEEEEECCCHHC
GTTSKRIVRMLREAGATEVHVRIASPPLKYPCFYGIDIQTRKELIAANHTVEEIREMIGA
CCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHHCCCHHHHHHHHCC
DSLTFLSEDGLVDAIGRPYEGKYGGLCMAYFNGDYPTALYDYEQELLESMK
CCEEEEECCCCHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]