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The map label for this gene is insK [H]

Identifier: 222094044

GI number: 222094044

Start: 308311

End: 309102

Strand: Direct

Name: insK [H]

Synonym: BCQ_0333

Alternate gene names: 222094044

Gene position: 308311-309102 (Clockwise)

Preceding gene: 222094043

Following gene: 222094046

Centisome position: 5.91

GC content: 37.75

Gene sequence:

>792_bases
TTGTGTAACGTATTAGAATTACCCCGGTCCACATTTTATCGTTGGTTACAACGAACGGAAGATCTAAAAGAGGATATAGA
AGAGAAAGTAAAGGATATTTGTCTTAGGCATAGATTCCGGTATGGATATCGAAGAGTGACCGCTACTCTTCGAAAAATGG
GACTGTGTGTCAATCATAAAAAAGTATTACGAATCATGAGACAAAATCATATTCTTTCAAAAGTACGTCGAAAGAAAAAG
AAATATATCAATGGTGCGGAACCAATGGTAGCCCCTCATCGATTGGAGCGCCAATTCGATGCATCCAGACCAAATGAAAA
GTGGTTTACGGATGTAACTTATTTATTATTTGGAGAGCGTACCTTGTATTTATCAACGATTATGGATGCCTTTAATCGTG
AAATTATTAGTTGTGTCATCAGCGAGTCGCAAACACTGACATTGGCGATGAAGACATTAAAACAAGCAATGAGAGGGCGA
AAAGTAAAAGGTGTCCTTCTTCACTCGGATCAAGGAAGTATTTATACAGCAAAAGAATTTCAAGCGTACGCCAAAGAAAA
CGGCATTATCACCAGCATGTCCCGGAAAGGAAATTGCCATGATAATGCCGTCATGGAAAGCTTCTTTGGTCATTTGAAAA
GTGAAGCTTTCTATTCACAAAAGATAACAAAAGTATCCAACACAACTGTGCGAAAGATTGTGCTAGAATACATTCATTAC
TACAATTGTGTGCGAATTCAAGAAAAATTAAACCACCTATCCCCTAAAGAATTTAGGGAACAAGTGGTTTAG

Upstream 100 bases:

>100_bases
TACTTGTATGGGGAAAGTATCTAGAAATCGAAAGGGGCTGAAACCAGAAACTGTAGTTACTTTATGGAACGATTTAAAAA
CAAAGCTAACGGTAAAAGAA

Downstream 100 bases:

>100_bases
GTGTTTTGATTAGTGTCCCGTTTTCAGGGTTCACTTCATATATTAGGTGTTTTTTTATTTGGCAAAGATAATAGATTGAA
ATCAGTATAGGTATTCTCTA

Product: ispsy9, transposase orfb

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MCNVLELPRSTFYRWLQRTEDLKEDIEEKVKDICLRHRFRYGYRRVTATLRKMGLCVNHKKVLRIMRQNHILSKVRRKKK
KYINGAEPMVAPHRLERQFDASRPNEKWFTDVTYLLFGERTLYLSTIMDAFNREIISCVISESQTLTLAMKTLKQAMRGR
KVKGVLLHSDQGSIYTAKEFQAYAKENGIITSMSRKGNCHDNAVMESFFGHLKSEAFYSQKITKVSNTTVRKIVLEYIHY
YNCVRIQEKLNHLSPKEFREQVV

Sequences:

>Translated_263_residues
MCNVLELPRSTFYRWLQRTEDLKEDIEEKVKDICLRHRFRYGYRRVTATLRKMGLCVNHKKVLRIMRQNHILSKVRRKKK
KYINGAEPMVAPHRLERQFDASRPNEKWFTDVTYLLFGERTLYLSTIMDAFNREIISCVISESQTLTLAMKTLKQAMRGR
KVKGVLLHSDQGSIYTAKEFQAYAKENGIITSMSRKGNCHDNAVMESFFGHLKSEAFYSQKITKVSNTTVRKIVLEYIHY
YNCVRIQEKLNHLSPKEFREQVV
>Mature_263_residues
MCNVLELPRSTFYRWLQRTEDLKEDIEEKVKDICLRHRFRYGYRRVTATLRKMGLCVNHKKVLRIMRQNHILSKVRRKKK
KYINGAEPMVAPHRLERQFDASRPNEKWFTDVTYLLFGERTLYLSTIMDAFNREIISCVISESQTLTLAMKTLKQAMRGR
KVKGVLLHSDQGSIYTAKEFQAYAKENGIITSMSRKGNCHDNAVMESFFGHLKSEAFYSQKITKVSNTTVRKIVLEYIHY
YNCVRIQEKLNHLSPKEFREQVV

Specific function: Involved in the transposition of the insertion sequence IS150 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=226, Percent_Identity=28.3185840707965, Blast_Score=93, Evalue=2e-19,
Organism=Escherichia coli, GI1789981, Length=260, Percent_Identity=43.4615384615385, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1788405, Length=267, Percent_Identity=34.4569288389513, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1787263, Length=267, Percent_Identity=34.4569288389513, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1786753, Length=267, Percent_Identity=34.4569288389513, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1786570, Length=267, Percent_Identity=34.4569288389513, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1786490, Length=267, Percent_Identity=34.4569288389513, Blast_Score=143, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31097; Mature: 31097

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCNVLELPRSTFYRWLQRTEDLKEDIEEKVKDICLRHRFRYGYRRVTATLRKMGLCVNHK
CCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
KVLRIMRQNHILSKVRRKKKKYINGAEPMVAPHRLERQFDASRPNEKWFTDVTYLLFGER
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCH
TLYLSTIMDAFNREIISCVISESQTLTLAMKTLKQAMRGRKVKGVLLHSDQGSIYTAKEF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEHHHH
QAYAKENGIITSMSRKGNCHDNAVMESFFGHLKSEAFYSQKITKVSNTTVRKIVLEYIHY
HHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YNCVRIQEKLNHLSPKEFREQVV
HHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MCNVLELPRSTFYRWLQRTEDLKEDIEEKVKDICLRHRFRYGYRRVTATLRKMGLCVNHK
CCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
KVLRIMRQNHILSKVRRKKKKYINGAEPMVAPHRLERQFDASRPNEKWFTDVTYLLFGER
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCH
TLYLSTIMDAFNREIISCVISESQTLTLAMKTLKQAMRGRKVKGVLLHSDQGSIYTAKEF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEHHHH
QAYAKENGIITSMSRKGNCHDNAVMESFFGHLKSEAFYSQKITKVSNTTVRKIVLEYIHY
HHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YNCVRIQEKLNHLSPKEFREQVV
HHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2841644; 2541046; 8041620; 9278503 [H]