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The map label for this gene is truA [H]

Identifier: 222093912

GI number: 222093912

Start: 138077

End: 138820

Strand: Direct

Name: truA [H]

Synonym: BCQ_0155

Alternate gene names: 222093912

Gene position: 138077-138820 (Clockwise)

Preceding gene: 222093911

Following gene: 222093913

Centisome position: 2.65

GC content: 37.5

Gene sequence:

>744_bases
ATGGATAGAATAAAATGTACGGTTGCATATGATGGCATGCATTTTTGTGGTTATCAAATTCAACCGCAGCATCGTACTGT
TCAACAAGAGATAGAGAAAGCTTTGCAGAAATTGCATAAAGGGGAACTTGTTCGTGTTCAGGCATCGGGCAGAACGGATT
CTACCGTTCATGCTAAAGGACAAGTGATACACTTTGATACACCGTTGTCTCTTGAAGAGTGGCAATGGAGCAATGCATTA
AACACTATGTTACCAGATGATATTGTTATCACACAGGTAGAGAAAAAAACAGAAGAATTTCACGCACGTTACGGTGTCGA
GAGAAAAGAATATCGTTATCGTGTATTATTATCAAAGACTGCGGATGTATTCCGTAGAAATTACGTATATCAATATCCAT
ATCCGCTCGAAATAAATGCTATAAGAAAAGCGATCCCTTATTTTATTGGAACGCATGATTTCACTTCTTTTTGTTCGGCA
AAAACTGACAAAAAAGATAAAGTGAGAACAATTTATGAAATCGAGCTAATTGAGCAGGACGACGAATTAATTTTTCGTTT
TGTAGGTAATGGATTTTTATATAATATGGTCAGGATTATTGTTGGTACGTTACTGAGCGTAGGGCAAGGAAAGCTTGATC
CGGATAGCATTCCAGAGATTTTAGCGAAACAAAATCGTCAGTTTGCTGGAAAGATGGCTCCTGGTCATGGGCTTTATTTA
TGGCAGGTAAACTATAACAACTAA

Upstream 100 bases:

>100_bases
GGCAACTACGCTGGAAAACAAGCGATACAGTAACGATTATAAGCTTACTTTGTTTAGCTTGTATTTTGGCATGGGTGCGA
TCGTAATGGAGAATAAAGAA

Downstream 100 bases:

>100_bases
TCCTGGTGTAACATTTGCTTGACATTAGAAACTCAAAAGTATATCATAACATATGGTATTGTTTCTAAAACCACGATTAG
CCCCGGAAGGAAATCGTGTT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I 1; tRNA-uridine isomerase I 1 [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MDRIKCTVAYDGMHFCGYQIQPQHRTVQQEIEKALQKLHKGELVRVQASGRTDSTVHAKGQVIHFDTPLSLEEWQWSNAL
NTMLPDDIVITQVEKKTEEFHARYGVERKEYRYRVLLSKTADVFRRNYVYQYPYPLEINAIRKAIPYFIGTHDFTSFCSA
KTDKKDKVRTIYEIELIEQDDELIFRFVGNGFLYNMVRIIVGTLLSVGQGKLDPDSIPEILAKQNRQFAGKMAPGHGLYL
WQVNYNN

Sequences:

>Translated_247_residues
MDRIKCTVAYDGMHFCGYQIQPQHRTVQQEIEKALQKLHKGELVRVQASGRTDSTVHAKGQVIHFDTPLSLEEWQWSNAL
NTMLPDDIVITQVEKKTEEFHARYGVERKEYRYRVLLSKTADVFRRNYVYQYPYPLEINAIRKAIPYFIGTHDFTSFCSA
KTDKKDKVRTIYEIELIEQDDELIFRFVGNGFLYNMVRIIVGTLLSVGQGKLDPDSIPEILAKQNRQFAGKMAPGHGLYL
WQVNYNN
>Mature_247_residues
MDRIKCTVAYDGMHFCGYQIQPQHRTVQQEIEKALQKLHKGELVRVQASGRTDSTVHAKGQVIHFDTPLSLEEWQWSNAL
NTMLPDDIVITQVEKKTEEFHARYGVERKEYRYRVLLSKTADVFRRNYVYQYPYPLEINAIRKAIPYFIGTHDFTSFCSA
KTDKKDKVRTIYEIELIEQDDELIFRFVGNGFLYNMVRIIVGTLLSVGQGKLDPDSIPEILAKQNRQFAGKMAPGHGLYL
WQVNYNN

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=249, Percent_Identity=30.9236947791165, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1788657, Length=243, Percent_Identity=32.0987654320988, Blast_Score=145, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6321112, Length=239, Percent_Identity=26.7782426778243, Blast_Score=72, Evalue=6e-14,
Organism=Drosophila melanogaster, GI116007752, Length=301, Percent_Identity=25.2491694352159, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281362146, Length=275, Percent_Identity=25.8181818181818, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28757; Mature: 28757

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRIKCTVAYDGMHFCGYQIQPQHRTVQQEIEKALQKLHKGELVRVQASGRTDSTVHAKG
CCCEEEEEEECCEEECCEEECCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCC
QVIHFDTPLSLEEWQWSNALNTMLPDDIVITQVEKKTEEFHARYGVERKEYRYRVLLSKT
EEEEECCCCCHHHHCHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCHHHHHEEHHHHHH
ADVFRRNYVYQYPYPLEINAIRKAIPYFIGTHDFTSFCSAKTDKKDKVRTIYEIELIEQD
HHHHHHCCEEECCCCEEHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHEEEEEEEEECCC
DELIFRFVGNGFLYNMVRIIVGTLLSVGQGKLDPDSIPEILAKQNRQFAGKMAPGHGLYL
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCEEEE
WQVNYNN
EEEECCC
>Mature Secondary Structure
MDRIKCTVAYDGMHFCGYQIQPQHRTVQQEIEKALQKLHKGELVRVQASGRTDSTVHAKG
CCCEEEEEEECCEEECCEEECCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCC
QVIHFDTPLSLEEWQWSNALNTMLPDDIVITQVEKKTEEFHARYGVERKEYRYRVLLSKT
EEEEECCCCCHHHHCHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCHHHHHEEHHHHHH
ADVFRRNYVYQYPYPLEINAIRKAIPYFIGTHDFTSFCSAKTDKKDKVRTIYEIELIEQD
HHHHHHCCEEECCCCEEHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHEEEEEEEEECCC
DELIFRFVGNGFLYNMVRIIVGTLLSVGQGKLDPDSIPEILAKQNRQFAGKMAPGHGLYL
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCEEEE
WQVNYNN
EEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]