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The map label for this gene is ispD [H]

Identifier: 222093856

GI number: 222093856

Start: 95781

End: 96461

Strand: Direct

Name: ispD [H]

Synonym: BCQ_0099

Alternate gene names: 222093856

Gene position: 95781-96461 (Clockwise)

Preceding gene: 222093855

Following gene: 222093857

Centisome position: 1.84

GC content: 39.65

Gene sequence:

>681_bases
ATGTATACATTAATTATTCCAGCAGCTGGTCAAGGAAAGCGGATGGGTGCTGGCAAAAATAAGTTGTTCTTACTTATTAA
TGAAGTACCGATTATTGTGCATACGTTACGTGCTTTCGAACAGGATAAAGCATGTAAAAATATTATTATGGCAATTAACG
AAGAAGAGCGTCCGTATTTTGAAGAGTTAATGCAAAAGTATCCGGTTGCAAAGCCAGTACAATTTATTCAAGGTGGAGCT
GAAAGACAAGATAGTGTATATAATGCGATTCAGCATACGAGTGATGTTGAGTATGTTCTTGTACATGACGGTGCGCGCCC
GTTCGTAACGAATAAAGTGATCCAAGACGTATTAACTGCAGCAGAAAAATATGGAGCTTCCATTTGTGCGGTGCCAGTGA
AAGATACTGTTAAGAAAGTAGAGCAGGGTGTTGTTGTCGAAACGGTAGAAAGATCTCAACTTAAAGCTGTTCAAACACCG
CAAGGGTTCTCTGTTTCTCTTTTGTTAGAAGCTCATAGAAGTGCGAAGCAGAGTTGTTTCCTTGGTACAGATGATGCAAG
TCTCGTAGAACGTATCGGGAAGCAAGTAGGTGTAGTAGAGGGGAGTTACTATAATATTAAAGTGACGACCCCAGAGGATT
TACTAATTGCTGAAAGTTTTCTTCACGTTCAAAAGAAATGA

Upstream 100 bases:

>100_bases
GATGTACTCGTTACGAGTGTGTTACAAACGTCGGCTGGGCGCATGATTTTTGCCAAACGTAAATTATTAGAAAAAGCATT
ATAAGTAGAGGATTATTAAT

Downstream 100 bases:

>100_bases
TAGCAATGATTATAGCGAAGAGAAGCTCGGCAAAGGCCGGGCTTTTCTTTATAAGGATAGCGATATAATATAGAATCATA
AAGCTCAGTAGTTTAGCTCG

Product: 2-c-methyl-d-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MYTLIIPAAGQGKRMGAGKNKLFLLINEVPIIVHTLRAFEQDKACKNIIMAINEEERPYFEELMQKYPVAKPVQFIQGGA
ERQDSVYNAIQHTSDVEYVLVHDGARPFVTNKVIQDVLTAAEKYGASICAVPVKDTVKKVEQGVVVETVERSQLKAVQTP
QGFSVSLLLEAHRSAKQSCFLGTDDASLVERIGKQVGVVEGSYYNIKVTTPEDLLIAESFLHVQKK

Sequences:

>Translated_226_residues
MYTLIIPAAGQGKRMGAGKNKLFLLINEVPIIVHTLRAFEQDKACKNIIMAINEEERPYFEELMQKYPVAKPVQFIQGGA
ERQDSVYNAIQHTSDVEYVLVHDGARPFVTNKVIQDVLTAAEKYGASICAVPVKDTVKKVEQGVVVETVERSQLKAVQTP
QGFSVSLLLEAHRSAKQSCFLGTDDASLVERIGKQVGVVEGSYYNIKVTTPEDLLIAESFLHVQKK
>Mature_226_residues
MYTLIIPAAGQGKRMGAGKNKLFLLINEVPIIVHTLRAFEQDKACKNIIMAINEEERPYFEELMQKYPVAKPVQFIQGGA
ERQDSVYNAIQHTSDVEYVLVHDGARPFVTNKVIQDVLTAAEKYGASICAVPVKDTVKKVEQGVVVETVERSQLKAVQTP
QGFSVSLLLEAHRSAKQSCFLGTDDASLVERIGKQVGVVEGSYYNIKVTTPEDLLIAESFLHVQKK

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=229, Percent_Identity=31.4410480349345, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI157671913, Length=132, Percent_Identity=34.0909090909091, Blast_Score=81, Evalue=9e-16,
Organism=Escherichia coli, GI1789104, Length=220, Percent_Identity=34.5454545454545, Blast_Score=119, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 25049; Mature: 25049

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTLIIPAAGQGKRMGAGKNKLFLLINEVPIIVHTLRAFEQDKACKNIIMAINEEERPYF
CEEEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
EELMQKYPVAKPVQFIQGGAERQDSVYNAIQHTSDVEYVLVHDGARPFVTNKVIQDVLTA
HHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHH
AEKYGASICAVPVKDTVKKVEQGVVVETVERSQLKAVQTPQGFSVSLLLEAHRSAKQSCF
HHHHCCEEEEECHHHHHHHHHCCHHEEHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHEE
LGTDDASLVERIGKQVGVVEGSYYNIKVTTPEDLLIAESFLHVQKK
CCCCCHHHHHHHHHHHCEECCCEEEEEEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYTLIIPAAGQGKRMGAGKNKLFLLINEVPIIVHTLRAFEQDKACKNIIMAINEEERPYF
CEEEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
EELMQKYPVAKPVQFIQGGAERQDSVYNAIQHTSDVEYVLVHDGARPFVTNKVIQDVLTA
HHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHH
AEKYGASICAVPVKDTVKKVEQGVVVETVERSQLKAVQTPQGFSVSLLLEAHRSAKQSCF
HHHHCCEEEEECHHHHHHHHHCCHHEEHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHEE
LGTDDASLVERIGKQVGVVEGSYYNIKVTTPEDLLIAESFLHVQKK
CCCCCHHHHHHHHHHHCEECCCEEEEEEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA