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The map label for this gene is spoVC [H]

Identifier: 222093821

GI number: 222093821

Start: 53354

End: 53914

Strand: Direct

Name: spoVC [H]

Synonym: BCQ_0058

Alternate gene names: 222093821

Gene position: 53354-53914 (Clockwise)

Preceding gene: 222093820

Following gene: 222093822

Centisome position: 1.02

GC content: 34.4

Gene sequence:

>561_bases
ATGAAATTGATAGTAGGACTTGGGAACCCAGGTAGAGAATATGAATTAACAAGGCATAATATTGGATTTATGGCGATTGA
TGAACTTGCAAAGCGTTGGAATATTTCTTTGAACGAACAAAAGTTTAAAGGAGTATTTGGTGCAGGATTTGTTAATGGAG
AAAAAGTAATCTTATTAAAGCCACTTACATATATGAATTTATCTGGAGAAAGTATTCGTCCGTTAATGGATTACTATAAA
ATTGATGTAGAGGACTTCGTTGTTCTGTACGATGATTTAGACATCCCTGTAGGTAAATTACGCCTTCGTATGAAAGGCAG
TGCGGGTGGACATAATGGTGTGAAATCAACAATTTCACATTTAGGAACACAAGAGTTTCAACGTATCCGCATGGGAATCG
ATCGTCCAAAAAATGGAATGAAGGTAGTAGATTACGTATTAGGACGTTTTACATCAGAAGAGATTCCTGATGTAAATCAT
TCTATTGAAAAAGCAGCAGATGCATGTGAAGAATGGTTAAATAAACCTTTTCTTCAAATTATGAATACTTTCAATAGTTA
A

Upstream 100 bases:

>100_bases
AGAATCTGTAAGTGTATTATTCAATTAATTGGATAGAATGAGACGTAACCAAGATTGGTTACGTCTTTTCGTATCGAAAA
AAGAAAGTAGTGGTACAAGA

Downstream 100 bases:

>100_bases
GAGTACGATTGGGAATATTTTATTTTATTTTTACCCATACTAGCAGTAATTGGATTTTTAGGAGGCTAGTATGGAAGGGT
ATTATTATTGCAGACATTGC

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MKLIVGLGNPGREYELTRHNIGFMAIDELAKRWNISLNEQKFKGVFGAGFVNGEKVILLKPLTYMNLSGESIRPLMDYYK
IDVEDFVVLYDDLDIPVGKLRLRMKGSAGGHNGVKSTISHLGTQEFQRIRMGIDRPKNGMKVVDYVLGRFTSEEIPDVNH
SIEKAADACEEWLNKPFLQIMNTFNS

Sequences:

>Translated_186_residues
MKLIVGLGNPGREYELTRHNIGFMAIDELAKRWNISLNEQKFKGVFGAGFVNGEKVILLKPLTYMNLSGESIRPLMDYYK
IDVEDFVVLYDDLDIPVGKLRLRMKGSAGGHNGVKSTISHLGTQEFQRIRMGIDRPKNGMKVVDYVLGRFTSEEIPDVNH
SIEKAADACEEWLNKPFLQIMNTFNS
>Mature_186_residues
MKLIVGLGNPGREYELTRHNIGFMAIDELAKRWNISLNEQKFKGVFGAGFVNGEKVILLKPLTYMNLSGESIRPLMDYYK
IDVEDFVVLYDDLDIPVGKLRLRMKGSAGGHNGVKSTISHLGTQEFQRIRMGIDRPKNGMKVVDYVLGRFTSEEIPDVNH
SIEKAADACEEWLNKPFLQIMNTFNS

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis [H]

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family [H]

Homologues:

Organism=Homo sapiens, GI50897284, Length=168, Percent_Identity=32.1428571428571, Blast_Score=103, Evalue=8e-23,
Organism=Escherichia coli, GI1787455, Length=188, Percent_Identity=39.3617021276596, Blast_Score=122, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321983, Length=176, Percent_Identity=30.1136363636364, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001328
- InterPro:   IPR018171 [H]

Pfam domain/function: PF01195 Pept_tRNA_hydro [H]

EC number: =3.1.1.29 [H]

Molecular weight: Translated: 21083; Mature: 21083

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1 ; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIVGLGNPGREYELTRHNIGFMAIDELAKRWNISLNEQKFKGVFGAGFVNGEKVILLK
CEEEEECCCCCCCEEEHHCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCEECCCEEEEEC
PLTYMNLSGESIRPLMDYYKIDVEDFVVLYDDLDIPVGKLRLRMKGSAGGHNGVKSTISH
CCEEECCCCCCHHHHHHHHHCCHHHEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHH
LGTQEFQRIRMGIDRPKNGMKVVDYVLGRFTSEEIPDVNHSIEKAADACEEWLNKPFLQI
HCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHH
MNTFNS
HHHHCC
>Mature Secondary Structure
MKLIVGLGNPGREYELTRHNIGFMAIDELAKRWNISLNEQKFKGVFGAGFVNGEKVILLK
CEEEEECCCCCCCEEEHHCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCEECCCEEEEEC
PLTYMNLSGESIRPLMDYYKIDVEDFVVLYDDLDIPVGKLRLRMKGSAGGHNGVKSTISH
CCEEECCCCCCHHHHHHHHHCCHHHEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHH
LGTQEFQRIRMGIDRPKNGMKVVDYVLGRFTSEEIPDVNHSIEKAADACEEWLNKPFLQI
HCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHH
MNTFNS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]