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The map label for this gene is metS [H]

Identifier: 222093808

GI number: 222093808

Start: 41487

End: 43367

Strand: Direct

Name: metS [H]

Synonym: BCQ_0045

Alternate gene names: 222093808

Gene position: 41487-43367 (Clockwise)

Preceding gene: 222093806

Following gene: 222093809

Centisome position: 0.8

GC content: 36.26

Gene sequence:

>1881_bases
ATGGCACGATATAAGCGTATGCAAGGATATAATGTTCATTATTTAACAGGAACTGATGAGCATGGACAGAAGATCCAAAA
GAAAGCAGAAGAATTAAATGTTACACCACAAGCATATGTGGATAACATCGTTGCAGGAATTAAAGAGCTTTGGGAGAAAA
TGGATATCTCTTATGATGATTTTATTCGTACAACGGAAGATCGTCATAAAGATGTTGTTGAAAAAATCTTCAAGCAACTA
GTAGATCAAGGCGATATTTACCTTGATGAATATGAAGGTTGGTATTCTGTACAAGATGAGACATTCTATACAGAGCACCA
ATTAGTAGATCCAATTATGGAAGGTGACAAAGTAGTTGGAGGAAAAAGTCCAGATAGTGGTCATGATGTAGAGCTTGTTC
GTGAAGAATCTTATTTCTTTAGAATGGGTAAATACGTAGACAGACTATTAAAATTCTATGAAGAGAATCCACATTTCATT
CAGCCAGAGTCTCGTAAGAATGAAATGATTAATAACTTTATTAAACCAGGATTGGAAGATTTAGCAGTTTCCCGTACTTC
ATTTGACTGGGGAGTTCGTGTTCCAGGTAATCCGAAACACGTTATTTACGTATGGGTTGACGCATTATCTAACTACATTA
CAGCATTAGGTTATGGAACAGAAAATGAAGAGATGTATAAGAAGTTCTGGCCAGCAGATGTGCATTTAGTTGGAAAAGAA
ATCGTTCGTTTCCATACGATTTATTGGCCAATCATTTTAATGGCATTAGATTTACCTCTTCCGAAAAAAGTCTTTGCTCA
CGGTTGGATTTTAATGAAGGATGGAAAGATGAGTAAGTCAAAAGGAAATGTAGTAGATCCAGTTACATTAATTGATCGTT
ACGGATTAGATGCATTACGTTATTACTTACTTCGTGAAGTTCCATTTGGATCTGATGGCGTATTCACACCAGAAGGATTT
GTGGAGCGTATTAATTTCGATTTAGCAAATGACTTAGGTAACTTATTAAATCGTACAGTAGCTATGATTGATAAGTACTT
TAACGGAGAAATCCCTGCATTTAAAGAAAATGTAACGGAATTTGATGAAACGTTAGTAACATTTGCGAAAGATACATTGA
AAAAAGTGGAAGAAGCAATGGAGAATATGGAATTCTCTGTAGCTCTAAGCTCAATTTGGCAATTGGTTAGCCGTACGAAT
AAATATATTGACGAAACACAACCGTGGGTATTAGCGAAAGATGAGAATGATCGCGAAAAGTTAGCCTCTGTAATGGCTCA
CTTAGCAGAAGTACTTCGTCAAACGGGTATTATGCTTATGCCATTCTTAACAGTAGCACCAAGCAAAATGTTTGCTCAGC
TTGGCCTTACTGATGAAGCACATATATCTTGGGGAAGCCTATCTACAATCGGCTGTATTCCAGCTGGAACAAAAGTAGAA
AAAGGACAACCAATTTTCCCTCGTTTAGAAATGGACGTGGAAGTGGCGTACATTAAAGAACAAATGAAAGCCTCTGCTCC
TAAAGTAGAAGAGAAAAAAGAAGAAGAACCGAAGGCAGAAGAAATTACAATTGATGATTTCTTTAAAGTAGAATTGCGTG
TAGCTGAAGTTCTATCTGCGGAACCTGTGAAAAAAGCAGACAAGTTGTTAAAAATTCAACTAGACTTAGGTACAGAAAAG
CGTCAAGTTGTTTCTGGAATTGCAAAATTCTATTCGCCAGAAGACCTAAAAGGTAAAAAGGTTATTTGTGTAACAAATTT
AAAACCTGTAAAATTACGCGGTGAATTATCGCAAGGTATGATTTTAGCGGGTGAAGAGAATGGCGTATTGTCATTAGCAT
CGATTGACCAAAATCTACCAAATGGTACAAAAATTAAGTAA

Upstream 100 bases:

>100_bases
GACAAAGGAAAACAAATCCTTTTATATTACTACCCCAATTTATTATCCAAGTGGAAAATTACACATTGGACATGCTTATA
CAACAGTAGCAGGAGATGCG

Downstream 100 bases:

>100_bases
TTAAGACAAGAAAAGAGATGTTTCACGTGTAACATGTGTGAAGCGTCTTTTTTCTTTTTACACTCTCAATTTTTGCATTA
AGATTGTAGTATTGTTATCG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase 1; MetRS 1 [H]

Number of amino acids: Translated: 626; Mature: 625

Protein sequence:

>626_residues
MARYKRMQGYNVHYLTGTDEHGQKIQKKAEELNVTPQAYVDNIVAGIKELWEKMDISYDDFIRTTEDRHKDVVEKIFKQL
VDQGDIYLDEYEGWYSVQDETFYTEHQLVDPIMEGDKVVGGKSPDSGHDVELVREESYFFRMGKYVDRLLKFYEENPHFI
QPESRKNEMINNFIKPGLEDLAVSRTSFDWGVRVPGNPKHVIYVWVDALSNYITALGYGTENEEMYKKFWPADVHLVGKE
IVRFHTIYWPIILMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPVTLIDRYGLDALRYYLLREVPFGSDGVFTPEGF
VERINFDLANDLGNLLNRTVAMIDKYFNGEIPAFKENVTEFDETLVTFAKDTLKKVEEAMENMEFSVALSSIWQLVSRTN
KYIDETQPWVLAKDENDREKLASVMAHLAEVLRQTGIMLMPFLTVAPSKMFAQLGLTDEAHISWGSLSTIGCIPAGTKVE
KGQPIFPRLEMDVEVAYIKEQMKASAPKVEEKKEEEPKAEEITIDDFFKVELRVAEVLSAEPVKKADKLLKIQLDLGTEK
RQVVSGIAKFYSPEDLKGKKVICVTNLKPVKLRGELSQGMILAGEENGVLSLASIDQNLPNGTKIK

Sequences:

>Translated_626_residues
MARYKRMQGYNVHYLTGTDEHGQKIQKKAEELNVTPQAYVDNIVAGIKELWEKMDISYDDFIRTTEDRHKDVVEKIFKQL
VDQGDIYLDEYEGWYSVQDETFYTEHQLVDPIMEGDKVVGGKSPDSGHDVELVREESYFFRMGKYVDRLLKFYEENPHFI
QPESRKNEMINNFIKPGLEDLAVSRTSFDWGVRVPGNPKHVIYVWVDALSNYITALGYGTENEEMYKKFWPADVHLVGKE
IVRFHTIYWPIILMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPVTLIDRYGLDALRYYLLREVPFGSDGVFTPEGF
VERINFDLANDLGNLLNRTVAMIDKYFNGEIPAFKENVTEFDETLVTFAKDTLKKVEEAMENMEFSVALSSIWQLVSRTN
KYIDETQPWVLAKDENDREKLASVMAHLAEVLRQTGIMLMPFLTVAPSKMFAQLGLTDEAHISWGSLSTIGCIPAGTKVE
KGQPIFPRLEMDVEVAYIKEQMKASAPKVEEKKEEEPKAEEITIDDFFKVELRVAEVLSAEPVKKADKLLKIQLDLGTEK
RQVVSGIAKFYSPEDLKGKKVICVTNLKPVKLRGELSQGMILAGEENGVLSLASIDQNLPNGTKIK
>Mature_625_residues
ARYKRMQGYNVHYLTGTDEHGQKIQKKAEELNVTPQAYVDNIVAGIKELWEKMDISYDDFIRTTEDRHKDVVEKIFKQLV
DQGDIYLDEYEGWYSVQDETFYTEHQLVDPIMEGDKVVGGKSPDSGHDVELVREESYFFRMGKYVDRLLKFYEENPHFIQ
PESRKNEMINNFIKPGLEDLAVSRTSFDWGVRVPGNPKHVIYVWVDALSNYITALGYGTENEEMYKKFWPADVHLVGKEI
VRFHTIYWPIILMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPVTLIDRYGLDALRYYLLREVPFGSDGVFTPEGFV
ERINFDLANDLGNLLNRTVAMIDKYFNGEIPAFKENVTEFDETLVTFAKDTLKKVEEAMENMEFSVALSSIWQLVSRTNK
YIDETQPWVLAKDENDREKLASVMAHLAEVLRQTGIMLMPFLTVAPSKMFAQLGLTDEAHISWGSLSTIGCIPAGTKVEK
GQPIFPRLEMDVEVAYIKEQMKASAPKVEEKKEEEPKAEEITIDDFFKVELRVAEVLSAEPVKKADKLLKIQLDLGTEKR
QVVSGIAKFYSPEDLKGKKVICVTNLKPVKLRGELSQGMILAGEENGVLSLASIDQNLPNGTKIK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=480, Percent_Identity=34.7916666666667, Blast_Score=277, Evalue=3e-74,
Organism=Homo sapiens, GI14043022, Length=625, Percent_Identity=25.76, Blast_Score=170, Evalue=3e-42,
Organism=Escherichia coli, GI1788432, Length=659, Percent_Identity=30.0455235204856, Blast_Score=268, Evalue=7e-73,
Organism=Escherichia coli, GI1789455, Length=108, Percent_Identity=34.2592592592593, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI212645096, Length=465, Percent_Identity=32.258064516129, Blast_Score=243, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI71992781, Length=387, Percent_Identity=32.5581395348837, Blast_Score=195, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI17540794, Length=563, Percent_Identity=25.044404973357, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321610, Length=456, Percent_Identity=30.9210526315789, Blast_Score=227, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6321704, Length=541, Percent_Identity=23.2902033271719, Blast_Score=116, Evalue=9e-27,
Organism=Drosophila melanogaster, GI21358483, Length=534, Percent_Identity=31.2734082397004, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24655311, Length=531, Percent_Identity=26.7419962335217, Blast_Score=167, Evalue=2e-41,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 71491; Mature: 71360

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYKRMQGYNVHYLTGTDEHGQKIQKKAEELNVTPQAYVDNIVAGIKELWEKMDISYDD
CCCCHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
FIRTTEDRHKDVVEKIFKQLVDQGDIYLDEYEGWYSVQDETFYTEHQLVDPIMEGDKVVG
HHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECCCCEEEHHHHHHHHHHCCCEEEC
GKSPDSGHDVELVREESYFFRMGKYVDRLLKFYEENPHFIQPESRKNEMINNFIKPGLED
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHCCCHHH
LAVSRTSFDWGVRVPGNPKHVIYVWVDALSNYITALGYGTENEEMYKKFWPADVHLVGKE
HHHCCCCCCCCEECCCCCCEEEEEEHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHH
IVRFHTIYWPIILMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPVTLIDRYGLDALR
HHHHHHHHHHHHHHHHCCCCCHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHCHHHHH
YYLLREVPFGSDGVFTPEGFVERINFDLANDLGNLLNRTVAMIDKYFNGEIPAFKENVTE
HHHHHCCCCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FDETLVTFAKDTLKKVEEAMENMEFSVALSSIWQLVSRTNKYIDETQPWVLAKDENDREK
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHH
LASVMAHLAEVLRQTGIMLMPFLTVAPSKMFAQLGLTDEAHISWGSLSTIGCIPAGTKVE
HHHHHHHHHHHHHHCCCEEEHHHHHCHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCC
KGQPIFPRLEMDVEVAYIKEQMKASAPKVEEKKEEEPKAEEITIDDFFKVELRVAEVLSA
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCC
EPVKKADKLLKIQLDLGTEKRQVVSGIAKFYSPEDLKGKKVICVTNLKPVKLRGELSQGM
CCHHHHHHEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEECCCCCCE
ILAGEENGVLSLASIDQNLPNGTKIK
EEECCCCCEEEEEHHHCCCCCCCCCC
>Mature Secondary Structure 
ARYKRMQGYNVHYLTGTDEHGQKIQKKAEELNVTPQAYVDNIVAGIKELWEKMDISYDD
CCCHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
FIRTTEDRHKDVVEKIFKQLVDQGDIYLDEYEGWYSVQDETFYTEHQLVDPIMEGDKVVG
HHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECCCCEEEHHHHHHHHHHCCCEEEC
GKSPDSGHDVELVREESYFFRMGKYVDRLLKFYEENPHFIQPESRKNEMINNFIKPGLED
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHCCCHHH
LAVSRTSFDWGVRVPGNPKHVIYVWVDALSNYITALGYGTENEEMYKKFWPADVHLVGKE
HHHCCCCCCCCEECCCCCCEEEEEEHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHH
IVRFHTIYWPIILMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPVTLIDRYGLDALR
HHHHHHHHHHHHHHHHCCCCCHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHCHHHHH
YYLLREVPFGSDGVFTPEGFVERINFDLANDLGNLLNRTVAMIDKYFNGEIPAFKENVTE
HHHHHCCCCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FDETLVTFAKDTLKKVEEAMENMEFSVALSSIWQLVSRTNKYIDETQPWVLAKDENDREK
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHH
LASVMAHLAEVLRQTGIMLMPFLTVAPSKMFAQLGLTDEAHISWGSLSTIGCIPAGTKVE
HHHHHHHHHHHHHHCCCEEEHHHHHCHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCC
KGQPIFPRLEMDVEVAYIKEQMKASAPKVEEKKEEEPKAEEITIDDFFKVELRVAEVLSA
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCC
EPVKKADKLLKIQLDLGTEKRQVVSGIAKFYSPEDLKGKKVICVTNLKPVKLRGELSQGM
CCHHHHHHEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEECCCCCCE
ILAGEENGVLSLASIDQNLPNGTKIK
EEECCCCCEEEEEHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]