Click here to switch to the map view.

The map label for this gene is cad [H]

Identifier: 222093799

GI number: 222093799

Start: 34313

End: 35734

Strand: Direct

Name: cad [H]

Synonym: BCQ_0036

Alternate gene names: 222093799

Gene position: 34313-35734 (Clockwise)

Preceding gene: 222093798

Following gene: 222093800

Centisome position: 0.66

GC content: 35.09

Gene sequence:

>1422_bases
ATGAATCAAAATCGCATACCTTTATATGAAGCATTAATAGAGTTTAAAGAAAGAAGGCCGTTATCCTTTCATGTCCCAGG
TCATAAAAATGGTTTAAACTTCCCTCAGGAGGCGGTAGGAGAGTTTAAAGATATACTATCTATCGATGTAACTGAGTTGT
CAGGATTAGATGATTTACATAGTCCGTTTGAATGTATAGATGAGGCACAACAATTATTAGCTGATGTATATGGAGTGAAC
AAAAGTTATTTTTTAATTAATGGCTCAACAGTCGGGAATCTAGCAATGATTTTGTCTTGTTGTGGGGAACATGATATTGT
TCTTGTACAAAGAAATTGTCATAAATCCATTATTAATGGTTTAAAGTTAGCGGGAGCAAATCCTATTTTTTTAGATCCAT
GGATTGATGAGGCTTATAACGTACCAGTGGGAATTCATGATGAGATTATTAAGGAAGCAATTGAAAAATATCCGAATGCG
AAAGCACTCATTTTAACGCATCCTAATTATTATGGTATGGGTATGGATTTAGAGGCAAGCATCGCTTATGCACATGCACA
TAAAATTCCTGTGTTAGTAGATGAAGCACATGGAGCTCATTTTTGTTTAGGCGGGGCATTTCCAAAGTCAGCGTTAGCAT
ATGGTGCAGACATTGTAGTTCATTCTGCGCATAAAACATTACCCGCAATGACGATGGGATCCTATTTACATATAAATAGT
CGCTTAGTGAAAGAAGAAAAAGTTTCTACTTATTTAAGTATGCTACAATCTAGTAGTCCATCGTATCCAATTATGACTTC
TCTTGATATTGCACGCTTTACGATAGCGCGTATAAAAGAAAAGGGACATGATGAAATTGTCGAGTTTTTACGGGAATTTA
AAGAAGAATTGAGTGCTATTCCACAAATAGCTATTTTACAATATCCATTACAAGACGAGCTAAAAGTTACCGTACAGACG
CGTTGTCAATTATCAGGATACGAATTACAGTCTTTATTTGAAAAGGTTGGTATATATACAGAAATGGCAGATCCATATAA
TGTCTTATTTATTTTGCCTTTGCAAATAAATAAGACATATATGAAGGCTATAGAGATGATCCGAGTGGCATTGCAATATT
ATGAGGTGAAAGACAAGACGGAATCTATTCGTTATACTTATAAAGGGGAAATTTCTCCTTTACCGTATACATATAAACAA
TTGGAAGAATACGAAACAAAAGTAGTATCTGTAGAAGAAGCAGTTGGGATGGTAGCAGCAGAAATGGTAATTCCGTATCC
ACCTGGTATTCCATTGATTATGTATGGGGAAAGAATTACTTCAGAACATAAAGAGCAAATTATGCATTTAGAGAAAGCTG
GCGCTCGTTTTCAAGGCAGCACGAAATATATGAAAGTGTATGATATAGAAAGTAGGTTTTAG

Upstream 100 bases:

>100_bases
TCGGAAATTAGAAGTATCATATTTTTAAATAGGCATATGCCTATTATTTTTTTTGTTCTGTATAATAGATAAAGAATGAA
ATAATAAGGAAGAGATATAG

Downstream 100 bases:

>100_bases
AATGAAGGGATTATTTGTAACAATTGAAGGTCCAGAAGGTTCGGGTAAAACAACGCTAATTCAAGGTTTATTGCCATACT
TTGAACAAAAGGAACAAAAG

Product: lysine decarboxylase

Products: cadaverine; CO2

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MNQNRIPLYEALIEFKERRPLSFHVPGHKNGLNFPQEAVGEFKDILSIDVTELSGLDDLHSPFECIDEAQQLLADVYGVN
KSYFLINGSTVGNLAMILSCCGEHDIVLVQRNCHKSIINGLKLAGANPIFLDPWIDEAYNVPVGIHDEIIKEAIEKYPNA
KALILTHPNYYGMGMDLEASIAYAHAHKIPVLVDEAHGAHFCLGGAFPKSALAYGADIVVHSAHKTLPAMTMGSYLHINS
RLVKEEKVSTYLSMLQSSSPSYPIMTSLDIARFTIARIKEKGHDEIVEFLREFKEELSAIPQIAILQYPLQDELKVTVQT
RCQLSGYELQSLFEKVGIYTEMADPYNVLFILPLQINKTYMKAIEMIRVALQYYEVKDKTESIRYTYKGEISPLPYTYKQ
LEEYETKVVSVEEAVGMVAAEMVIPYPPGIPLIMYGERITSEHKEQIMHLEKAGARFQGSTKYMKVYDIESRF

Sequences:

>Translated_473_residues
MNQNRIPLYEALIEFKERRPLSFHVPGHKNGLNFPQEAVGEFKDILSIDVTELSGLDDLHSPFECIDEAQQLLADVYGVN
KSYFLINGSTVGNLAMILSCCGEHDIVLVQRNCHKSIINGLKLAGANPIFLDPWIDEAYNVPVGIHDEIIKEAIEKYPNA
KALILTHPNYYGMGMDLEASIAYAHAHKIPVLVDEAHGAHFCLGGAFPKSALAYGADIVVHSAHKTLPAMTMGSYLHINS
RLVKEEKVSTYLSMLQSSSPSYPIMTSLDIARFTIARIKEKGHDEIVEFLREFKEELSAIPQIAILQYPLQDELKVTVQT
RCQLSGYELQSLFEKVGIYTEMADPYNVLFILPLQINKTYMKAIEMIRVALQYYEVKDKTESIRYTYKGEISPLPYTYKQ
LEEYETKVVSVEEAVGMVAAEMVIPYPPGIPLIMYGERITSEHKEQIMHLEKAGARFQGSTKYMKVYDIESRF
>Mature_473_residues
MNQNRIPLYEALIEFKERRPLSFHVPGHKNGLNFPQEAVGEFKDILSIDVTELSGLDDLHSPFECIDEAQQLLADVYGVN
KSYFLINGSTVGNLAMILSCCGEHDIVLVQRNCHKSIINGLKLAGANPIFLDPWIDEAYNVPVGIHDEIIKEAIEKYPNA
KALILTHPNYYGMGMDLEASIAYAHAHKIPVLVDEAHGAHFCLGGAFPKSALAYGADIVVHSAHKTLPAMTMGSYLHINS
RLVKEEKVSTYLSMLQSSSPSYPIMTSLDIARFTIARIKEKGHDEIVEFLREFKEELSAIPQIAILQYPLQDELKVTVQT
RCQLSGYELQSLFEKVGIYTEMADPYNVLFILPLQINKTYMKAIEMIRVALQYYEVKDKTESIRYTYKGEISPLPYTYKQ
LEEYETKVVSVEEAVGMVAAEMVIPYPPGIPLIMYGERITSEHKEQIMHLEKAGARFQGSTKYMKVYDIESRF

Specific function: Appears To Play A Role In pH Homeostasis By Consuming Protons And Neutralizing The Acidic By-Products Of Carbohydrate Fermentation. [C]

COG id: COG1982

COG function: function code E; Arginine/lysine/ornithine decarboxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Orn/Lys/Arg decarboxylase class-I family [H]

Homologues:

Organism=Escherichia coli, GI1790573, Length=565, Percent_Identity=24.070796460177, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1786384, Length=548, Percent_Identity=22.992700729927, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI221142684, Length=414, Percent_Identity=23.6714975845411, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87082193, Length=309, Percent_Identity=23.6245954692557, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786909, Length=144, Percent_Identity=32.6388888888889, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000310
- InterPro:   IPR008286
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF01276 OKR_DC_1; PF03711 OKR_DC_1_C [H]

EC number: 4.1.1.18

Molecular weight: Translated: 53443; Mature: 53443

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQNRIPLYEALIEFKERRPLSFHVPGHKNGLNFPQEAVGEFKDILSIDVTELSGLDDLH
CCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHEEHHHHCCCHHHC
SPFECIDEAQQLLADVYGVNKSYFLINGSTVGNLAMILSCCGEHDIVLVQRNCHKSIING
CHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEECCHHHHHHCC
LKLAGANPIFLDPWIDEAYNVPVGIHDEIIKEAIEKYPNAKALILTHPNYYGMGMDLEAS
CEECCCCCEEEECCHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHH
IAYAHAHKIPVLVDEAHGAHFCLGGAFPKSALAYGADIVVHSAHKTLPAMTMGSYLHINS
EEEHHHCCCCEEEECCCCCEEEECCCCCHHHHHCCCCEEEECCHHCCCHHHCCCEEEECH
RLVKEEKVSTYLSMLQSSSPSYPIMTSLDIARFTIARIKEKGHDEIVEFLREFKEELSAI
HHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
PQIAILQYPLQDELKVTVQTRCQLSGYELQSLFEKVGIYTEMADPYNVLFILPLQINKTY
CCEEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHCCEEECCCCCEEEEEEEEECCHHH
MKAIEMIRVALQYYEVKDKTESIRYTYKGEISPLPYTYKQLEEYETKVVSVEEAVGMVAA
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EMVIPYPPGIPLIMYGERITSEHKEQIMHLEKAGARFQGSTKYMKVYDIESRF
HEEECCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MNQNRIPLYEALIEFKERRPLSFHVPGHKNGLNFPQEAVGEFKDILSIDVTELSGLDDLH
CCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHEEHHHHCCCHHHC
SPFECIDEAQQLLADVYGVNKSYFLINGSTVGNLAMILSCCGEHDIVLVQRNCHKSIING
CHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEECCHHHHHHCC
LKLAGANPIFLDPWIDEAYNVPVGIHDEIIKEAIEKYPNAKALILTHPNYYGMGMDLEAS
CEECCCCCEEEECCHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHH
IAYAHAHKIPVLVDEAHGAHFCLGGAFPKSALAYGADIVVHSAHKTLPAMTMGSYLHINS
EEEHHHCCCCEEEECCCCCEEEECCCCCHHHHHCCCCEEEECCHHCCCHHHCCCEEEECH
RLVKEEKVSTYLSMLQSSSPSYPIMTSLDIARFTIARIKEKGHDEIVEFLREFKEELSAI
HHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
PQIAILQYPLQDELKVTVQTRCQLSGYELQSLFEKVGIYTEMADPYNVLFILPLQINKTY
CCEEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHCCEEECCCCCEEEEEEEEECCHHH
MKAIEMIRVALQYYEVKDKTESIRYTYKGEISPLPYTYKQLEEYETKVVSVEEAVGMVAA
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EMVIPYPPGIPLIMYGERITSEHKEQIMHLEKAGARFQGSTKYMKVYDIESRF
HEEECCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate; Sulfhydryl groups [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.4 {S-aminoethyl-L-Cys}} 2 {L-Lys}} 1.5 {L-Lys}} 7 {delta-hydroxylysine}} [C]

Substrates: L-lysine

Specific reaction: L-lysine = cadaverine + CO2

General reaction: Decarboxylation [C]

Inhibitor: NaBH4; NaCl [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]