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The map label for this gene is dnaN [H]

Identifier: 222093776

GI number: 222093776

Start: 1927

End: 3066

Strand: Direct

Name: dnaN [H]

Synonym: BCQ_0002

Alternate gene names: 222093776

Gene position: 1927-3066 (Clockwise)

Preceding gene: 222093775

Following gene: 222093777

Centisome position: 0.04

GC content: 32.54

Gene sequence:

>1140_bases
ATGCGTTTTACAATACAAAAAGACTATCTTGTAAGAAGTGTACAAGATGTAATGAAGGCTGTTTCTTCTCGTACAACAAT
TCCGATCCTTACGGGGATTAAAGTTGTCGCTACAGAAGAAGGAGTTACATTAACAGGAAGCGATGCTGATATTTCAATTG
AATCTTTCATTCCAGTTGAAGAGAATGGAAAAGAAATCGTAGAAGTGAATCAATCAGGAAGTATTGTTTTACAGGCTAAA
TATTTTAGTGAAATTGTAAAGAAGTTGCCTAAAGAAACTGTAGAAATTTCTGTGGAAAATCATTTGATGACAAAAATAAC
TTCTGGAAAATCAGAGTTTAATTTAAATGGTTTAGATTCTGCAGAATATCCATTGTTACCACAAATTGAAGAACATCATG
TTTTTAAGATTCCAACGGATTTACTTAAACATATGATTAGACAAACTGTATTTGCAGTTTCCACTTCTGAAACAAGACCA
ATCTTGACAGGTGTAAACTGGAAGGTATATAACAGCGAACTAACTGGCATTGCTACAGATAGTCACAGACTAGCTCTTCG
AAAAGCAAAAATTGAAGGCATTGCAGATGAGTTCCAAGCAAATGTTGTTATTCCAGGAAAAAGTTTAAATGAATTAAGCA
AAATTCTAGATGAGTCTGAAGAAATGGTAGATATCGTTATTACGGAGTATCAAGTATTATTCCGTACAAAACATTTATTA
TTCTTCTCAAGATTGTTAGAAGGAAATTACCCTGATACAACTCGCTTAATTCCAGCGGAGAGCAAAACAGATATTTTTGT
AAATACAAAAGAATTTTTACAAGCAATTGATCGTGCATCTCTTTTAGCAAGAGATGGTCGTAATAATGTTGTAAAATTAT
CAACTTTAGAGCAGGCAATGCTAGAAATTTCCTCGAATTCACCAGAAATCGGAAAAGTAGTAGAAGAAGTTCAATGTGAA
AAAGTAGATGGAGAAGAGTTAAAAATATCTTTTAGTGCAAAATATATGATGGATGCACTAAAAGCATTAGATAGTACTGA
AATTAAGATTAGCTTTACTGGAGCAATGAGACCATTCTTAATTCGTACAGTAAATGATGAATCCATTATTCAATTAATTT
TACCGGTTCGTACTTACTAA

Upstream 100 bases:

>100_bases
AACAGAGTTATCCACATATCCACAAGCCCTATTACTATTACTACTATTTTTTATCTTTATTAATTAAATAAAATCTTATA
CTTACCGGAGGTTTTTCTTT

Downstream 100 bases:

>100_bases
GTAAGAAATAAGGGTTGCTAGTTTTCAGATGCTAGTAGCCCTTATTTGATTTTTGGGTATTACTTTCCTAATGCTAGTTT
ATTTAGTACAATGAAAGAAT

Product: DNA polymerase iii subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MRFTIQKDYLVRSVQDVMKAVSSRTTIPILTGIKVVATEEGVTLTGSDADISIESFIPVEENGKEIVEVNQSGSIVLQAK
YFSEIVKKLPKETVEISVENHLMTKITSGKSEFNLNGLDSAEYPLLPQIEEHHVFKIPTDLLKHMIRQTVFAVSTSETRP
ILTGVNWKVYNSELTGIATDSHRLALRKAKIEGIADEFQANVVIPGKSLNELSKILDESEEMVDIVITEYQVLFRTKHLL
FFSRLLEGNYPDTTRLIPAESKTDIFVNTKEFLQAIDRASLLARDGRNNVVKLSTLEQAMLEISSNSPEIGKVVEEVQCE
KVDGEELKISFSAKYMMDALKALDSTEIKISFTGAMRPFLIRTVNDESIIQLILPVRTY

Sequences:

>Translated_379_residues
MRFTIQKDYLVRSVQDVMKAVSSRTTIPILTGIKVVATEEGVTLTGSDADISIESFIPVEENGKEIVEVNQSGSIVLQAK
YFSEIVKKLPKETVEISVENHLMTKITSGKSEFNLNGLDSAEYPLLPQIEEHHVFKIPTDLLKHMIRQTVFAVSTSETRP
ILTGVNWKVYNSELTGIATDSHRLALRKAKIEGIADEFQANVVIPGKSLNELSKILDESEEMVDIVITEYQVLFRTKHLL
FFSRLLEGNYPDTTRLIPAESKTDIFVNTKEFLQAIDRASLLARDGRNNVVKLSTLEQAMLEISSNSPEIGKVVEEVQCE
KVDGEELKISFSAKYMMDALKALDSTEIKISFTGAMRPFLIRTVNDESIIQLILPVRTY
>Mature_379_residues
MRFTIQKDYLVRSVQDVMKAVSSRTTIPILTGIKVVATEEGVTLTGSDADISIESFIPVEENGKEIVEVNQSGSIVLQAK
YFSEIVKKLPKETVEISVENHLMTKITSGKSEFNLNGLDSAEYPLLPQIEEHHVFKIPTDLLKHMIRQTVFAVSTSETRP
ILTGVNWKVYNSELTGIATDSHRLALRKAKIEGIADEFQANVVIPGKSLNELSKILDESEEMVDIVITEYQVLFRTKHLL
FFSRLLEGNYPDTTRLIPAESKTDIFVNTKEFLQAIDRASLLARDGRNNVVKLSTLEQAMLEISSNSPEIGKVVEEVQCE
KVDGEELKISFSAKYMMDALKALDSTEIKISFTGAMRPFLIRTVNDESIIQLILPVRTY

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=379, Percent_Identity=26.6490765171504, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42515; Mature: 42515

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFTIQKDYLVRSVQDVMKAVSSRTTIPILTGIKVVATEEGVTLTGSDADISIESFIPVE
CEEEECHHHHHHHHHHHHHHHHCCCCEEEEECEEEEEECCCEEEECCCCCEEEEEECCCC
ENGKEIVEVNQSGSIVLQAKYFSEIVKKLPKETVEISVENHLMTKITSGKSEFNLNGLDS
CCCCEEEEECCCCCEEEEEHHHHHHHHHCCCHHEEEEECCCEEEEEECCCCCCCCCCCCC
AEYPLLPQIEEHHVFKIPTDLLKHMIRQTVFAVSTSETRPILTGVNWKVYNSELTGIATD
CCCCCCCCCCCCCEEECCHHHHHHHHHHHHHEEECCCCCCEEECCEEEEECCEECEEECC
SHRLALRKAKIEGIADEFQANVVIPGKSLNELSKILDESEEMVDIVITEYQVLFRTKHLL
CHHHHHHHHHHHCCHHHHCCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
FFSRLLEGNYPDTTRLIPAESKTDIFVNTKEFLQAIDRASLLARDGRNNVVKLSTLEQAM
HHHHHHCCCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHH
LEISSNSPEIGKVVEEVQCEKVDGEELKISFSAKYMMDALKALDSTEIKISFTGAMRPFL
HHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEEEECCCCCEE
IRTVNDESIIQLILPVRTY
EEECCCHHHEEEEECCCCC
>Mature Secondary Structure
MRFTIQKDYLVRSVQDVMKAVSSRTTIPILTGIKVVATEEGVTLTGSDADISIESFIPVE
CEEEECHHHHHHHHHHHHHHHHCCCCEEEEECEEEEEECCCEEEECCCCCEEEEEECCCC
ENGKEIVEVNQSGSIVLQAKYFSEIVKKLPKETVEISVENHLMTKITSGKSEFNLNGLDS
CCCCEEEEECCCCCEEEEEHHHHHHHHHCCCHHEEEEECCCEEEEEECCCCCCCCCCCCC
AEYPLLPQIEEHHVFKIPTDLLKHMIRQTVFAVSTSETRPILTGVNWKVYNSELTGIATD
CCCCCCCCCCCCCEEECCHHHHHHHHHHHHHEEECCCCCCEEECCEEEEECCEECEEECC
SHRLALRKAKIEGIADEFQANVVIPGKSLNELSKILDESEEMVDIVITEYQVLFRTKHLL
CHHHHHHHHHHHCCHHHHCCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
FFSRLLEGNYPDTTRLIPAESKTDIFVNTKEFLQAIDRASLLARDGRNNVVKLSTLEQAM
HHHHHHCCCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHH
LEISSNSPEIGKVVEEVQCEKVDGEELKISFSAKYMMDALKALDSTEIKISFTGAMRPFL
HHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEEEECCCCCEE
IRTVNDESIIQLILPVRTY
EEECCCHHHEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377; 2846289 [H]