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The map label for this gene is puuB [H]

Identifier: 221636298

GI number: 221636298

Start: 873062

End: 874471

Strand: Reverse

Name: puuB [H]

Synonym: trd_A0892

Alternate gene names: 221636298

Gene position: 874471-873062 (Counterclockwise)

Preceding gene: 221636299

Following gene: 221636297

Centisome position: 95.29

GC content: 65.96

Gene sequence:

>1410_bases
ATGGCCGGGACACTCGACCCGCGACGCGTGTATGGTCAGTACAGCTTCTGGCTGGAAACGTGCGGTGACGACTTGTCTCC
GCGCCCCGCCCTCGAGGGGACCTACGAGGCCGATGTCGCCATTCTCGGCGCTGGCTTTACGGGCTTGTGGACGGCCTATT
ACCTGCTCGAGCGTGAGCCGGGCTTGAAGGTCGTTATCCTGGAACAGCGGATCGCTGGCTTCGGCGCCTCCGGACGCAAC
GGGGGATGGCTCTATCCGGGGTTTCCTGTTTCGCTCGGGGAGTTGGCTGAACGCTACGGTGAGGAGACGGCGCGTGCGAT
CCACTACGCCATGCGTGCGACAGTCGACGAAGTCGAGCGTGTGCTCCAGGTCGAGGGGATCGACGCGCATTTTCACCGGG
GCGGTGTCCTCCGGCTCGCTCGCGGGGCGCATCAAGTGCCTGCTCTGGAGGAAACGGCACGCACGCTCGAGCGTCTCGGC
ATCACCGACTATGCCGAATGGCTGTCAGTCGAGGCGGTTCGTCAGCGCGTCCGGGTGACCAAGGTGGTGGCTGGGTTGTA
CTCGGCGCATGGAGCGGTCATCCACCCGGGTCGACTGGTGCGTGGCCTGGCCCGCGCGCTGGAGCGGCGCGGTGCCGTGA
TCTTCGAGGAGACGCCGGTCGTCGGCTTCCGAGGAGGCAGTTCTCCCTTCTTCCGTACACCGCGAGGGGAAGTCCGGGCA
CGCGTGCTCGTCCTGGCTGGCGAGGCGTATCTCTCGCAGCTAGCTCCCTTGCGGCGGACACTGATTCCGATCTACTCGCT
GATCGTGCTCACCGAGCCTGTTCCGGACGAGGTGTGGCAGGAGATCGGCTGGGAGAACCGCGAACTGCTCTCATCGCAGC
GGCTCACCGTTGATTACCTGCAGCGGACCGCGGACGGTCGCATCATGTTCGGTGGGCGCGGCGCGCCCTATCGCTTCGGC
TCGCGCATCGCCGATGCCTACGATCGGCATGGTCCGACCCACGAGGGGTTGCGGCAGATGTGCTACGAGTGGTTCCCTGC
GCTCCGCCTCCATGGTGTGCGCTTCACGCATGCTTGGGGTGGACCGCTCGGGGTGCCTCGCGACTGGATGCCGACCATGC
GCTACGATCCGCGCAGCGGTATCGCGCTCGCTTGCGGCTATACCGGACAGGGGGTGGCGACAGCGAACTTGGCCGGACGC
ATCCTGGCCGACCTGATCACCGGGCAGGAGAGCGAGCTGACGCGATTACCGATGACCAGTCATCGCCTGAAGGATTGGGA
ACCGGAACCGTTGCGCTGGCTCGGTGTGCGCTTCGTTCAGCTCGGCTATGAGCGACTGGATCGGATCGCGGAGGCGACCG
GCAAGGCACCGACCGGGCGCAGCTTGGTCGAGCGACTGGCAGCACACTGA

Upstream 100 bases:

>100_bases
GGGCCGTGCCTCACGGGCTGCTGGTGCACCCGATACCTGCGTCGCGCTTGTTCCGCGCTTGGCGCTGGGTCATACTGCGA
GCGGTAGGGGAGGACGAGCC

Downstream 100 bases:

>100_bases
GGTGGATGACACGAGTGAAATCCTCGCTCACCGGAGGGATGGGAGCCTAACGCGCGAGTGGTGCATGGTGCGACCGGCCG
GTGGAGTGCGGAGGTCCGAT

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MAGTLDPRRVYGQYSFWLETCGDDLSPRPALEGTYEADVAILGAGFTGLWTAYYLLEREPGLKVVILEQRIAGFGASGRN
GGWLYPGFPVSLGELAERYGEETARAIHYAMRATVDEVERVLQVEGIDAHFHRGGVLRLARGAHQVPALEETARTLERLG
ITDYAEWLSVEAVRQRVRVTKVVAGLYSAHGAVIHPGRLVRGLARALERRGAVIFEETPVVGFRGGSSPFFRTPRGEVRA
RVLVLAGEAYLSQLAPLRRTLIPIYSLIVLTEPVPDEVWQEIGWENRELLSSQRLTVDYLQRTADGRIMFGGRGAPYRFG
SRIADAYDRHGPTHEGLRQMCYEWFPALRLHGVRFTHAWGGPLGVPRDWMPTMRYDPRSGIALACGYTGQGVATANLAGR
ILADLITGQESELTRLPMTSHRLKDWEPEPLRWLGVRFVQLGYERLDRIAEATGKAPTGRSLVERLAAH

Sequences:

>Translated_469_residues
MAGTLDPRRVYGQYSFWLETCGDDLSPRPALEGTYEADVAILGAGFTGLWTAYYLLEREPGLKVVILEQRIAGFGASGRN
GGWLYPGFPVSLGELAERYGEETARAIHYAMRATVDEVERVLQVEGIDAHFHRGGVLRLARGAHQVPALEETARTLERLG
ITDYAEWLSVEAVRQRVRVTKVVAGLYSAHGAVIHPGRLVRGLARALERRGAVIFEETPVVGFRGGSSPFFRTPRGEVRA
RVLVLAGEAYLSQLAPLRRTLIPIYSLIVLTEPVPDEVWQEIGWENRELLSSQRLTVDYLQRTADGRIMFGGRGAPYRFG
SRIADAYDRHGPTHEGLRQMCYEWFPALRLHGVRFTHAWGGPLGVPRDWMPTMRYDPRSGIALACGYTGQGVATANLAGR
ILADLITGQESELTRLPMTSHRLKDWEPEPLRWLGVRFVQLGYERLDRIAEATGKAPTGRSLVERLAAH
>Mature_468_residues
AGTLDPRRVYGQYSFWLETCGDDLSPRPALEGTYEADVAILGAGFTGLWTAYYLLEREPGLKVVILEQRIAGFGASGRNG
GWLYPGFPVSLGELAERYGEETARAIHYAMRATVDEVERVLQVEGIDAHFHRGGVLRLARGAHQVPALEETARTLERLGI
TDYAEWLSVEAVRQRVRVTKVVAGLYSAHGAVIHPGRLVRGLARALERRGAVIFEETPVVGFRGGSSPFFRTPRGEVRAR
VLVLAGEAYLSQLAPLRRTLIPIYSLIVLTEPVPDEVWQEIGWENRELLSSQRLTVDYLQRTADGRIMFGGRGAPYRFGS
RIADAYDRHGPTHEGLRQMCYEWFPALRLHGVRFTHAWGGPLGVPRDWMPTMRYDPRSGIALACGYTGQGVATANLAGRI
LADLITGQESELTRLPMTSHRLKDWEPEPLRWLGVRFVQLGYERLDRIAEATGKAPTGRSLVERLAAH

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI24797151, Length=443, Percent_Identity=23.4762979683973, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1787559, Length=410, Percent_Identity=29.2682926829268, Blast_Score=152, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 52237; Mature: 52106

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTLDPRRVYGQYSFWLETCGDDLSPRPALEGTYEADVAILGAGFTGLWTAYYLLEREP
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCC
GLKVVILEQRIAGFGASGRNGGWLYPGFPVSLGELAERYGEETARAIHYAMRATVDEVER
CCEEEEEECHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLQVEGIDAHFHRGGVLRLARGAHQVPALEETARTLERLGITDYAEWLSVEAVRQRVRVT
HHHHCCCCHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KVVAGLYSAHGAVIHPGRLVRGLARALERRGAVIFEETPVVGFRGGSSPFFRTPRGEVRA
HHHHHHHHCCCCEECCHHHHHHHHHHHHHCCCEEEECCCEEEECCCCCCCCCCCCHHHEE
RVLVLAGEAYLSQLAPLRRTLIPIYSLIVLTEPVPDEVWQEIGWENRELLSSQRLTVDYL
EEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHCCCHHHHH
QRTADGRIMFGGRGAPYRFGSRIADAYDRHGPTHEGLRQMCYEWFPALRLHGVRFTHAWG
HHCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECC
GPLGVPRDWMPTMRYDPRSGIALACGYTGQGVATANLAGRILADLITGQESELTRLPMTS
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHCCCCCHHEECCCCH
HRLKDWEPEPLRWLGVRFVQLGYERLDRIAEATGKAPTGRSLVERLAAH
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
AGTLDPRRVYGQYSFWLETCGDDLSPRPALEGTYEADVAILGAGFTGLWTAYYLLEREP
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCC
GLKVVILEQRIAGFGASGRNGGWLYPGFPVSLGELAERYGEETARAIHYAMRATVDEVER
CCEEEEEECHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLQVEGIDAHFHRGGVLRLARGAHQVPALEETARTLERLGITDYAEWLSVEAVRQRVRVT
HHHHCCCCHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KVVAGLYSAHGAVIHPGRLVRGLARALERRGAVIFEETPVVGFRGGSSPFFRTPRGEVRA
HHHHHHHHCCCCEECCHHHHHHHHHHHHHCCCEEEECCCEEEECCCCCCCCCCCCHHHEE
RVLVLAGEAYLSQLAPLRRTLIPIYSLIVLTEPVPDEVWQEIGWENRELLSSQRLTVDYL
EEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHCCCHHHHH
QRTADGRIMFGGRGAPYRFGSRIADAYDRHGPTHEGLRQMCYEWFPALRLHGVRFTHAWG
HHCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECC
GPLGVPRDWMPTMRYDPRSGIALACGYTGQGVATANLAGRILADLITGQESELTRLPMTS
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHCCCCCHHEECCCCH
HRLKDWEPEPLRWLGVRFVQLGYERLDRIAEATGKAPTGRSLVERLAAH
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]