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The map label for this gene is kipI [H]

Identifier: 221636176

GI number: 221636176

Start: 750944

End: 751936

Strand: Reverse

Name: kipI [H]

Synonym: trd_A0770

Alternate gene names: 221636176

Gene position: 751936-750944 (Counterclockwise)

Preceding gene: 221636177

Following gene: 221636175

Centisome position: 81.93

GC content: 62.34

Gene sequence:

>993_bases
ATGATGGGCACTGGTCCTCATCCCTCAGGAGTGGTCCTGGCGAGCTTCCCGGAGCGGAACGGCCGCCCGAGCGTGGTGTA
CCGCAATGCGGGAGACAGCTTCCTCTTGGTCGAGTTCGGCGAGATGGTTTTCGACCTGACGATGAGCTTCCGCGTGCTGG
GGCTGGACGATGCGATCAAGCGACATCGTCCGGCAGGACTCATCGAGACGATCCCGGCCCTGCGCAGTATCTTGATCAAC
TACGATAGTCGACAGATCGGGGTGCGCGATCTGATCGATTTCGTCGAGGACCAATACGAGCATCTGCCCCCGTTCGAGAA
CCTCGTGGTGCCGAGCCGGATCGTCGAACTGCCGATCGCCTTCGACGACAAGTGGACGCGCGAAGCGATCGCTCGCTACG
TCCAGTACCAGCGAGCCGATGCACCGAACGTGATCGACGGAACGAACTCCAAGTACGCGGCCATGTACAACGGACTGCGC
GATGAGGAAGAGTTCTTCAGCTACATCATGGCGACCGACTGGTGGAATGCTGCACTCGGCTTCTTCCCCGGTCTGCCGTT
CGCCTATCCGTTGGATCCGCGCTATGCGGTCGTCCTGCCGAAGTACAACCCGACGCGACACTGGACACCGGCTGGAACGG
TCGGTATCGCCGGACCGTGCTTGGCCATCTATCCGGTGGAATCGGCCGGAGGCTATCAGATCTTCGGGCATACGGTGCCG
ATCTACGATCCGCAACAGCGCAATCCAGCTTTCCGCGAGAGTCCCGTCCTCCTACGCCCAGGCGACCGCCTGCGCTTCCG
CCCACGGGTGACCGACGACGAACTGGAGGCGATGATCCAAGCGGTCTACGACGGCACGTACCAGTACCGGATCGACGAGA
CGGCGACCTTCGACGTTGGTGCGTATCTCAAGTTCCTCAAAGAGGTGGAGCCGGAAGCGGAGGCGTTCCGGCGTCGCCAG
GCCGAGGCGGCTGCTCGCGTGCCGGTTCCGTGA

Upstream 100 bases:

>100_bases
TTCCTGGTGGAGAACGAAGAGGCGATCCGACCCGGCCAACCGATCGCGGTGGTGAGCGACGAGGCCTGACCCCGGTAGGC
AGCGGAAGGAGCGAGCGGTC

Downstream 100 bases:

>100_bases
TGCTCGCGAGAAGGCAGGAAGGGTGGAGGAGCCATGCTCGAGGTACGGAACGGCGGCTTCGAAACGACGGTACAGGACTA
TCCGGGGCGGATCGGGATGC

Product: urea amidolyase

Products: ADP; phosphate; urea-1-carboxylate

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MMGTGPHPSGVVLASFPERNGRPSVVYRNAGDSFLLVEFGEMVFDLTMSFRVLGLDDAIKRHRPAGLIETIPALRSILIN
YDSRQIGVRDLIDFVEDQYEHLPPFENLVVPSRIVELPIAFDDKWTREAIARYVQYQRADAPNVIDGTNSKYAAMYNGLR
DEEEFFSYIMATDWWNAALGFFPGLPFAYPLDPRYAVVLPKYNPTRHWTPAGTVGIAGPCLAIYPVESAGGYQIFGHTVP
IYDPQQRNPAFRESPVLLRPGDRLRFRPRVTDDELEAMIQAVYDGTYQYRIDETATFDVGAYLKFLKEVEPEAEAFRRRQ
AEAAARVPVP

Sequences:

>Translated_330_residues
MMGTGPHPSGVVLASFPERNGRPSVVYRNAGDSFLLVEFGEMVFDLTMSFRVLGLDDAIKRHRPAGLIETIPALRSILIN
YDSRQIGVRDLIDFVEDQYEHLPPFENLVVPSRIVELPIAFDDKWTREAIARYVQYQRADAPNVIDGTNSKYAAMYNGLR
DEEEFFSYIMATDWWNAALGFFPGLPFAYPLDPRYAVVLPKYNPTRHWTPAGTVGIAGPCLAIYPVESAGGYQIFGHTVP
IYDPQQRNPAFRESPVLLRPGDRLRFRPRVTDDELEAMIQAVYDGTYQYRIDETATFDVGAYLKFLKEVEPEAEAFRRRQ
AEAAARVPVP
>Mature_330_residues
MMGTGPHPSGVVLASFPERNGRPSVVYRNAGDSFLLVEFGEMVFDLTMSFRVLGLDDAIKRHRPAGLIETIPALRSILIN
YDSRQIGVRDLIDFVEDQYEHLPPFENLVVPSRIVELPIAFDDKWTREAIARYVQYQRADAPNVIDGTNSKYAAMYNGLR
DEEEFFSYIMATDWWNAALGFFPGLPFAYPLDPRYAVVLPKYNPTRHWTPAGTVGIAGPCLAIYPVESAGGYQIFGHTVP
IYDPQQRNPAFRESPVLLRPGDRLRFRPRVTDDELEAMIQAVYDGTYQYRIDETATFDVGAYLKFLKEVEPEAEAFRRRQ
AEAAARVPVP

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=206, Percent_Identity=28.1553398058252, Blast_Score=77, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319685, Length=311, Percent_Identity=33.1189710610932, Blast_Score=171, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: 6.3.4.6

Molecular weight: Translated: 37391; Mature: 37391

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMGTGPHPSGVVLASFPERNGRPSVVYRNAGDSFLLVEFGEMVFDLTMSFRVLGLDDAIK
CCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCEEEEEHHHHHHHHHHHEEEECCHHHHH
RHRPAGLIETIPALRSILINYDSRQIGVRDLIDFVEDQYEHLPPFENLVVPSRIVELPIA
HCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCHHHEEECCC
FDDKWTREAIARYVQYQRADAPNVIDGTNSKYAAMYNGLRDEEEFFSYIMATDWWNAALG
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
FFPGLPFAYPLDPRYAVVLPKYNPTRHWTPAGTVGIAGPCLAIYPVESAGGYQIFGHTVP
HCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEECCEEC
IYDPQQRNPAFRESPVLLRPGDRLRFRPRVTDDELEAMIQAVYDGTYQYRIDETATFDVG
CCCCCCCCCCCCCCCEEECCCCCEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCEECHH
AYLKFLKEVEPEAEAFRRRQAEAAARVPVP
HHHHHHHHCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMGTGPHPSGVVLASFPERNGRPSVVYRNAGDSFLLVEFGEMVFDLTMSFRVLGLDDAIK
CCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCEEEEEHHHHHHHHHHHEEEECCHHHHH
RHRPAGLIETIPALRSILINYDSRQIGVRDLIDFVEDQYEHLPPFENLVVPSRIVELPIA
HCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCHHHEEECCC
FDDKWTREAIARYVQYQRADAPNVIDGTNSKYAAMYNGLRDEEEFFSYIMATDWWNAALG
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
FFPGLPFAYPLDPRYAVVLPKYNPTRHWTPAGTVGIAGPCLAIYPVESAGGYQIFGHTVP
HCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEECCEEC
IYDPQQRNPAFRESPVLLRPGDRLRFRPRVTDDELEAMIQAVYDGTYQYRIDETATFDVG
CCCCCCCCCCCCCCCEEECCCCCEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCEECHH
AYLKFLKEVEPEAEAFRRRQAEAAARVPVP
HHHHHHHHCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; urea; CO2

Specific reaction: ATP + urea + CO2 = ADP + phosphate + urea-1-carboxylate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]