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The map label for this gene is sigB [H]

Identifier: 221636011

GI number: 221636011

Start: 595797

End: 596801

Strand: Reverse

Name: sigB [H]

Synonym: trd_A0605

Alternate gene names: 221636011

Gene position: 596801-595797 (Counterclockwise)

Preceding gene: 221636012

Following gene: 221636006

Centisome position: 65.03

GC content: 63.58

Gene sequence:

>1005_bases
ATGACGGAACGTGAACTGCAGGAACTGCTGGAACTCGAAGAACTGATCGCGTCGGAAGGGCTCGAGGAGGTCGAAGAGAT
CGAGGAGATCGAAGAGTCCGACGATCTCATCGATCAGGCGGCCAACGATGCGGTGTACCGCTATTTCCGTGAGGTGGGGG
GTCACCGGTTGCTGACCCACCAGGAGGAGATCGAGTACTCCCGGGCCGTACGGGCTGGTTTGGCGGCCCGCAAGCGGATC
GAGGCCGGTGAAGACACTCCTGAGCTGCGCGAACTCGTGCGCAAGGGGCAGGAGGCGCGCGAAAAGCTGATCCGGCACAA
CCTGCGCCTGGTCGTCTCGATCGCCAAGCGTTACCGCTCGAGCGAACTGCCGCTGATCGATCTGATCCAGGAGGGGAACA
TCGGTCTGATGACGGCGGTGGAGCGGTACGATCCGGAACTGGGTTACCGCTTCAGCACCTATGCGACCTTCTGGATCCGC
CAGGCGATCGGGCGTGCCGTGGCCAACTTGAGCCGAACGATCCGGGTTCCCGTCCACATGCATGACTTGATCGCCAAGAT
CCGGCGCGCGGAGGCACAGATCGAGCAGCAGAAGGGTCGACCAGCGACGAACGAGGAACTGGCTGAACTGTTGGGCATCG
ATGTGGCGCGGATCGAGCAGGCCCGAACGCAGATTCCGCGAACGTCCTCGCTCGACAAGCCGATCGGTGAGGATGGCGAG
AGCTCGATCGGTGATCTTTTGCCTGATCCGAGGAGCGATGACGTGGTCGAGGAAGCGTTGACCAATGCGATCCGCGAGCA
GATCCGCCGCAGCCTGGAGCAGCTCACGGAGCGCGAGCGCACCGTCCTGGTGATGCGCTTCGGTCTGGACGGCGAGCAGC
CGCGCACCCTGGCGGAAATCGCCGATGCGCTGAAGATCAGCCGCGAGCGTGTCCGCCAGGTCGAGAAGGAGGCGCTGCAG
AAGCTGCGCAACTCCGACCTGCGCTTGCTGGCGAGCGCGGCCTGA

Upstream 100 bases:

>100_bases
TTGACGCTGGCGTACGGTGTGTGCTAGTATATGTAGGTTTCGAAACGTCGTCGTGCGGTGTCCGTACGACGCGTCCGAAC
AACGGAGGTACGAGAGACCC

Downstream 100 bases:

>100_bases
CATTGACGATCTCTCGACGTCGAGCGGGCCGGTCTCCCGAAAAAGGGAGTCCGGCCCGATTCGTTCAGTCGTGATCGTGC
TGATGCTCGGGGTCGTGCCA

Product: RNA polymerase sigma factor RpoD (Sigma-A) (Sigma-43)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTERELQELLELEELIASEGLEEVEEIEEIEESDDLIDQAANDAVYRYFREVGGHRLLTHQEEIEYSRAVRAGLAARKRI
EAGEDTPELRELVRKGQEAREKLIRHNLRLVVSIAKRYRSSELPLIDLIQEGNIGLMTAVERYDPELGYRFSTYATFWIR
QAIGRAVANLSRTIRVPVHMHDLIAKIRRAEAQIEQQKGRPATNEELAELLGIDVARIEQARTQIPRTSSLDKPIGEDGE
SSIGDLLPDPRSDDVVEEALTNAIREQIRRSLEQLTERERTVLVMRFGLDGEQPRTLAEIADALKISRERVRQVEKEALQ
KLRNSDLRLLASAA

Sequences:

>Translated_334_residues
MTERELQELLELEELIASEGLEEVEEIEEIEESDDLIDQAANDAVYRYFREVGGHRLLTHQEEIEYSRAVRAGLAARKRI
EAGEDTPELRELVRKGQEAREKLIRHNLRLVVSIAKRYRSSELPLIDLIQEGNIGLMTAVERYDPELGYRFSTYATFWIR
QAIGRAVANLSRTIRVPVHMHDLIAKIRRAEAQIEQQKGRPATNEELAELLGIDVARIEQARTQIPRTSSLDKPIGEDGE
SSIGDLLPDPRSDDVVEEALTNAIREQIRRSLEQLTERERTVLVMRFGLDGEQPRTLAEIADALKISRERVRQVEKEALQ
KLRNSDLRLLASAA
>Mature_333_residues
TERELQELLELEELIASEGLEEVEEIEEIEESDDLIDQAANDAVYRYFREVGGHRLLTHQEEIEYSRAVRAGLAARKRIE
AGEDTPELRELVRKGQEAREKLIRHNLRLVVSIAKRYRSSELPLIDLIQEGNIGLMTAVERYDPELGYRFSTYATFWIRQ
AIGRAVANLSRTIRVPVHMHDLIAKIRRAEAQIEQQKGRPATNEELAELLGIDVARIEQARTQIPRTSSLDKPIGEDGES
SIGDLLPDPRSDDVVEEALTNAIREQIRRSLEQLTERERTVLVMRFGLDGEQPRTLAEIADALKISRERVRQVEKEALQK
LRNSDLRLLASAA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=230, Percent_Identity=44.3478260869565, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1789098, Length=295, Percent_Identity=36.6101694915254, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1789871, Length=245, Percent_Identity=33.469387755102, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788231, Length=225, Percent_Identity=33.7777777777778, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 38158; Mature: 38027

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERELQELLELEELIASEGLEEVEEIEEIEESDDLIDQAANDAVYRYFREVGGHRLLTH
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCC
QEEIEYSRAVRAGLAARKRIEAGEDTPELRELVRKGQEAREKLIRHNLRLVVSIAKRYRS
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SELPLIDLIQEGNIGLMTAVERYDPELGYRFSTYATFWIRQAIGRAVANLSRTIRVPVHM
CCCCHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
HDLIAKIRRAEAQIEQQKGRPATNEELAELLGIDVARIEQARTQIPRTSSLDKPIGEDGE
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
SSIGDLLPDPRSDDVVEEALTNAIREQIRRSLEQLTERERTVLVMRFGLDGEQPRTLAEI
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHH
ADALKISRERVRQVEKEALQKLRNSDLRLLASAA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure 
TERELQELLELEELIASEGLEEVEEIEEIEESDDLIDQAANDAVYRYFREVGGHRLLTH
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCC
QEEIEYSRAVRAGLAARKRIEAGEDTPELRELVRKGQEAREKLIRHNLRLVVSIAKRYRS
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SELPLIDLIQEGNIGLMTAVERYDPELGYRFSTYATFWIRQAIGRAVANLSRTIRVPVHM
CCCCHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
HDLIAKIRRAEAQIEQQKGRPATNEELAELLGIDVARIEQARTQIPRTSSLDKPIGEDGE
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
SSIGDLLPDPRSDDVVEEALTNAIREQIRRSLEQLTERERTVLVMRFGLDGEQPRTLAEI
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHH
ADALKISRERVRQVEKEALQKLRNSDLRLLASAA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910; 2116013 [H]