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The map label for this gene is ybiK [C]

Identifier: 221635927

GI number: 221635927

Start: 513889

End: 514848

Strand: Reverse

Name: ybiK [C]

Synonym: trd_A0521

Alternate gene names: 221635927

Gene position: 514848-513889 (Counterclockwise)

Preceding gene: 221635928

Following gene: 221635926

Centisome position: 56.1

GC content: 63.96

Gene sequence:

>960_bases
ATGCCGAAGGAGATCCGCGGTATCGTCGTCGGCAGCCGCAACGCTGCGGTCGGGTTCCCGCGAGCTATCGAGATTTTGCG
GGGCGGCGGCAGTGCCCTCGAGGCAGCGATCGCTGCGGTGAAAGCCGTCGAGGACGACCTTTGCGACCAGGGAGTCGGAA
CTGGTGGTATCCCGAATATCCTCGGCGAAGTCGAACTCGATGCCAGCGTCATGGACGGTCGGACGCTCGCTGCTGGTGCA
GTCGGCGCACTCAAGCATTACCCGCACCCGATCGAAGTCGCCCGCCGGATCATGGAGTGTACGCCGCATGTCATGCTCGT
CGGTGAGGGCGCCGAACTCTTCGCGCGCACCCATGGGTTCCAGCCCGCGAATCTCCTCACACCGGAGGCTGAGGCGATCT
GGCGGGCCGTGATCCATGGGGAGCGGAGGACGGAGCACAATGTCTACGAAGATCGGTACCAGCTCTACATGTCGGTCGTC
CAGCGATGGCGCGAGTTACTTCACCGCGAGCTCTTCGGTACGACCAACGTGATCGTGCGCGACCTCGCTGGCAACATCGC
GTGTGCGGTCTCGACGAGCGGATGGGGTTTCAAGTGGCCGGGGCGTCTGGGCGACTCACCGATCATCGGTGCCGGTAACT
ACGCGGACAACCGGTACGGAGCAGCAGCCTGTACCGGACGTGGAGAGATGGCGATCCGCTGTGCGACCGCGCACAGCGTC
GTCATGTACTTGCGGTTCGGTTTCACCCTCGAGGATGCACTCCGCCAGGCGATGCTGGATCTCCGCGCCCTGGACGATCC
CTATGCCGAGGCCGATACCGTCATGAACATCGTCGCGATGGATCGTGACGGCAACGTTAGTGCGGCATCGACGTCGCCGG
AGGCGACGTACGTCGTCCAGACGATCGACATGGAGACGTACGAGGAACGGCCTCGCCTGCACGTGCCGCTCCGCGAATGA

Upstream 100 bases:

>100_bases
GAGCAGCGCTGACGACGGTGTGCTGTATCTCCGCTGGTGGCGTGACGAGTTCGAGCGCGCAGCCCACGGGGCGACTGGAA
CGGAACGCTGAGGGGGTAAG

Downstream 100 bases:

>100_bases
ACACACGCTGAATCGCCACTGGCCAGGATTCGGTGGGCAGGGTGTGGTCCACTCCGATGAGCCGCCCGAACGTGGGCGTG
CGGAGTCGCACCGGTGGCCT

Product: isoaspartyl peptidase (ecaiii) (beta-aspartyl-peptidase) (isoaspartyl dipeptidase)

Products: NA

Alternate protein names: Aspartylglucosaminidase; AGA; Glycosylasparaginase; N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MPKEIRGIVVGSRNAAVGFPRAIEILRGGGSALEAAIAAVKAVEDDLCDQGVGTGGIPNILGEVELDASVMDGRTLAAGA
VGALKHYPHPIEVARRIMECTPHVMLVGEGAELFARTHGFQPANLLTPEAEAIWRAVIHGERRTEHNVYEDRYQLYMSVV
QRWRELLHRELFGTTNVIVRDLAGNIACAVSTSGWGFKWPGRLGDSPIIGAGNYADNRYGAAACTGRGEMAIRCATAHSV
VMYLRFGFTLEDALRQAMLDLRALDDPYAEADTVMNIVAMDRDGNVSAASTSPEATYVVQTIDMETYEERPRLHVPLRE

Sequences:

>Translated_319_residues
MPKEIRGIVVGSRNAAVGFPRAIEILRGGGSALEAAIAAVKAVEDDLCDQGVGTGGIPNILGEVELDASVMDGRTLAAGA
VGALKHYPHPIEVARRIMECTPHVMLVGEGAELFARTHGFQPANLLTPEAEAIWRAVIHGERRTEHNVYEDRYQLYMSVV
QRWRELLHRELFGTTNVIVRDLAGNIACAVSTSGWGFKWPGRLGDSPIIGAGNYADNRYGAAACTGRGEMAIRCATAHSV
VMYLRFGFTLEDALRQAMLDLRALDDPYAEADTVMNIVAMDRDGNVSAASTSPEATYVVQTIDMETYEERPRLHVPLRE
>Mature_318_residues
PKEIRGIVVGSRNAAVGFPRAIEILRGGGSALEAAIAAVKAVEDDLCDQGVGTGGIPNILGEVELDASVMDGRTLAAGAV
GALKHYPHPIEVARRIMECTPHVMLVGEGAELFARTHGFQPANLLTPEAEAIWRAVIHGERRTEHNVYEDRYQLYMSVVQ
RWRELLHRELFGTTNVIVRDLAGNIACAVSTSGWGFKWPGRLGDSPIIGAGNYADNRYGAAACTGRGEMAIRCATAHSVV
MYLRFGFTLEDALRQAMLDLRALDDPYAEADTVMNIVAMDRDGNVSAASTSPEATYVVQTIDMETYEERPRLHVPLRE

Specific function: Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins. Requires that the glycosylated asparagine moiety is not substituted on its N- (R1) and C- (R2) terminus [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=241, Percent_Identity=38.5892116182573, Blast_Score=138, Evalue=5e-33,
Organism=Homo sapiens, GI145275200, Length=241, Percent_Identity=38.5892116182573, Blast_Score=138, Evalue=5e-33,
Organism=Homo sapiens, GI285002251, Length=238, Percent_Identity=38.2352941176471, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI98991769, Length=313, Percent_Identity=32.2683706070287, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI285002253, Length=238, Percent_Identity=36.1344537815126, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1787050, Length=267, Percent_Identity=35.9550561797753, Blast_Score=133, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17569239, Length=262, Percent_Identity=32.0610687022901, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI221330102, Length=305, Percent_Identity=34.4262295081967, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24652135, Length=301, Percent_Identity=34.5514950166113, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI18921183, Length=224, Percent_Identity=35.7142857142857, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24655553, Length=248, Percent_Identity=32.258064516129, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI21357893, Length=289, Percent_Identity=29.757785467128, Blast_Score=85, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.5.1.26 [H]

Molecular weight: Translated: 34704; Mature: 34573

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKEIRGIVVGSRNAAVGFPRAIEILRGGGSALEAAIAAVKAVEDDLCDQGVGTGGIPNI
CCCCEEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LGEVELDASVMDGRTLAAGAVGALKHYPHPIEVARRIMECTPHVMLVGEGAELFARTHGF
HCCEEECCCHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHCCC
QPANLLTPEAEAIWRAVIHGERRTEHNVYEDRYQLYMSVVQRWRELLHRELFGTTNVIVR
CCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
DLAGNIACAVSTSGWGFKWPGRLGDSPIIGAGNYADNRYGAAACTGRGEMAIRCATAHSV
HHCCCEEEEEECCCCCEECCCCCCCCCEEECCCCCCCCCCCEEECCCCCEEEEEEHHHHH
VMYLRFGFTLEDALRQAMLDLRALDDPYAEADTVMNIVAMDRDGNVSAASTSPEATYVVQ
HHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEECCCCCEEECCCCCCCEEEEE
TIDMETYEERPRLHVPLRE
EECHHHHHCCCCEECCCCC
>Mature Secondary Structure 
PKEIRGIVVGSRNAAVGFPRAIEILRGGGSALEAAIAAVKAVEDDLCDQGVGTGGIPNI
CCCEEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LGEVELDASVMDGRTLAAGAVGALKHYPHPIEVARRIMECTPHVMLVGEGAELFARTHGF
HCCEEECCCHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHCCC
QPANLLTPEAEAIWRAVIHGERRTEHNVYEDRYQLYMSVVQRWRELLHRELFGTTNVIVR
CCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
DLAGNIACAVSTSGWGFKWPGRLGDSPIIGAGNYADNRYGAAACTGRGEMAIRCATAHSV
HHCCCEEEEEECCCCCEECCCCCCCCCEEECCCCCCCCCCCEEECCCCCEEEEEEHHHHH
VMYLRFGFTLEDALRQAMLDLRALDDPYAEADTVMNIVAMDRDGNVSAASTSPEATYVVQ
HHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEECCCCCEEECCCCCCCEEEEE
TIDMETYEERPRLHVPLRE
EECHHHHHCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7840643; 8250923; 9541410 [H]