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The map label for this gene is lplA [H]

Identifier: 221635914

GI number: 221635914

Start: 499455

End: 500234

Strand: Reverse

Name: lplA [H]

Synonym: trd_A0508

Alternate gene names: 221635914

Gene position: 500234-499455 (Counterclockwise)

Preceding gene: 221635915

Following gene: 221635908

Centisome position: 54.51

GC content: 63.59

Gene sequence:

>780_bases
ATGACCGAGCTGGAACGCTGGAAGCGGTACCGCTGGCAGTTGATCGCTGGGGAAGCGTTCGATCCAGCGCTGCAGATGGC
ACTCGACGAGGTGCTGACACGTCGAGTCGGAGCTGGCGAGCGGCCTCCCACGCTCCGCTTCTGGGAATGGACTGCCCCCG
CGGTCGTGATCGGTCGCTTCCAGTCGCTCCGCAACGAGGTCGATTTCGCCGAGGCCGAGCGATACGGAATCACGGTCGTC
CGCCGCATCACCGGTGGCGGCGCCATGCTGACCGAGCCCGGCAAAGTGATCACCTATTCGATCTACGCGCCGCCGGAACT
GGTCGCCGGGATGTCTTTCCAGGAATCGTACGCCTTTCTCGACCGGTGGGTTGTCGAGGCGTTGCGGGCACTCGGCGTCG
ATGCCTGGTACCAGCCGATCAACGACATCGCTTCAGCGCGTGGCAAGATCGGCGGTGCAGCGCAAGCCCGCCGCTATGGA
GCCGTCTTGCATCACACGACCATGGCTTACGATATCGATCCCGAAAAGGTTCCGCGCGTCATCCGCATCGGACGCGAGAA
ACTCAGCGACAAAGGAGTGCCGAGCGCAGCCAAGCGAGTCGCCCCATTGCGGCAGCAGACCGATCTGCCGCGCGAGGTGA
TCCAGGAACACCTCATCCGCACGTTCGCTGAACGACACGGCCTCGAGGAAGGCATGCTCCTTCCCGAAGAGATCGAAGAG
GCCCGCGAACTCGTCCGGACCAAGTTTGGAACGTGGGAGTGGACAGCGATCCTGCCCTGA

Upstream 100 bases:

>100_bases
CCCAGCGGATCCAGCGTGCACTCGAACGCTTCGGTGACGGCCTGGAACTGCTCGGCTTCTCTCCCGAAGCCGTCGCACGA
GCGACCCGGAGGGCGATCGA

Downstream 100 bases:

>100_bases
GGGCTACTGGAACAACTTCGAGCCTGTTCCCCGTCGCCGGCGACCTTCTTCCAGGATCGCTGCGATCTCTGCCTTGCTGA
ATAGTCGCTCGCAGAAGGTG

Product: biotin/lipoate A/B protein ligase

Products: NA

Alternate protein names: Lipoate--protein ligase subunit 1 [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MTELERWKRYRWQLIAGEAFDPALQMALDEVLTRRVGAGERPPTLRFWEWTAPAVVIGRFQSLRNEVDFAEAERYGITVV
RRITGGGAMLTEPGKVITYSIYAPPELVAGMSFQESYAFLDRWVVEALRALGVDAWYQPINDIASARGKIGGAAQARRYG
AVLHHTTMAYDIDPEKVPRVIRIGREKLSDKGVPSAAKRVAPLRQQTDLPREVIQEHLIRTFAERHGLEEGMLLPEEIEE
ARELVRTKFGTWEWTAILP

Sequences:

>Translated_259_residues
MTELERWKRYRWQLIAGEAFDPALQMALDEVLTRRVGAGERPPTLRFWEWTAPAVVIGRFQSLRNEVDFAEAERYGITVV
RRITGGGAMLTEPGKVITYSIYAPPELVAGMSFQESYAFLDRWVVEALRALGVDAWYQPINDIASARGKIGGAAQARRYG
AVLHHTTMAYDIDPEKVPRVIRIGREKLSDKGVPSAAKRVAPLRQQTDLPREVIQEHLIRTFAERHGLEEGMLLPEEIEE
ARELVRTKFGTWEWTAILP
>Mature_258_residues
TELERWKRYRWQLIAGEAFDPALQMALDEVLTRRVGAGERPPTLRFWEWTAPAVVIGRFQSLRNEVDFAEAERYGITVVR
RITGGGAMLTEPGKVITYSIYAPPELVAGMSFQESYAFLDRWVVEALRALGVDAWYQPINDIASARGKIGGAAQARRYGA
VLHHTTMAYDIDPEKVPRVIRIGREKLSDKGVPSAAKRVAPLRQQTDLPREVIQEHLIRTFAERHGLEEGMLLPEEIEEA
RELVRTKFGTWEWTAILP

Specific function: Lipoate-protein ligase catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes. This subunit can alone only catalyze the lipoat

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Escherichia coli, GI1790846, Length=252, Percent_Identity=25.7936507936508, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 29427; Mature: 29296

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELERWKRYRWQLIAGEAFDPALQMALDEVLTRRVGAGERPPTLRFWEWTAPAVVIGRF
CCHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHH
QSLRNEVDFAEAERYGITVVRRITGGGAMLTEPGKVITYSIYAPPELVAGMSFQESYAFL
HHHHHHCCHHHHHHHHHHEEEEECCCCCEEECCCCEEEEEEECCHHHHHCCCHHHHHHHH
DRWVVEALRALGVDAWYQPINDIASARGKIGGAAQARRYGAVLHHTTMAYDIDPEKVPRV
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEECCHHHHHHH
IRIGREKLSDKGVPSAAKRVAPLRQQTDLPREVIQEHLIRTFAERHGLEEGMLLPEEIEE
HHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
ARELVRTKFGTWEWTAILP
HHHHHHHHCCCEEEEEECC
>Mature Secondary Structure 
TELERWKRYRWQLIAGEAFDPALQMALDEVLTRRVGAGERPPTLRFWEWTAPAVVIGRF
CHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHH
QSLRNEVDFAEAERYGITVVRRITGGGAMLTEPGKVITYSIYAPPELVAGMSFQESYAFL
HHHHHHCCHHHHHHHHHHEEEEECCCCCEEECCCCEEEEEEECCHHHHHCCCHHHHHHHH
DRWVVEALRALGVDAWYQPINDIASARGKIGGAAQARRYGAVLHHTTMAYDIDPEKVPRV
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEECCHHHHHHH
IRIGREKLSDKGVPSAAKRVAPLRQQTDLPREVIQEHLIRTFAERHGLEEGMLLPEEIEE
HHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
ARELVRTKFGTWEWTAILP
HHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11029001 [H]