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The map label for this gene is merA [H]

Identifier: 221635786

GI number: 221635786

Start: 365005

End: 366687

Strand: Reverse

Name: merA [H]

Synonym: trd_A0380

Alternate gene names: 221635786

Gene position: 366687-365005 (Counterclockwise)

Preceding gene: 221635787

Following gene: 221635783

Centisome position: 39.96

GC content: 67.38

Gene sequence:

>1683_bases
ATGACACCAGCGAACCACGAATCGGCCCTCGTCACGTACGAACTCGACGTGGAAGGGATGACCTGCCGGGATTGTGAGCG
GCACGTCGAGACAGCTGTCGAGGCCTCTGGCGGTATCGAGCCGCGAGCGGACTGGCGACGCCGTCGCGTCCGCTTCGCTG
CCCCGCCCACTTTCGACCCCCAACCTGTCTTCGCTGCCATTCGTGCTGCTAGCGGACAACTGCATCGGTACGAGCCAGGG
CAACTGCGGCGCCTGGCGCCTGTCCAGCTGGACGATGAGTCACCCGACGCTGATGCCGACCTGGTGATCATCGGCGGTGG
CTCAGCCGCCTTCGCTGCCGCGATCGAGGCCCACCAGCTCGGTGCCCGCGTCATCATGATCGAACGTGGCACGCTGGGTG
GAACCTGCGTCAACATCGGGTGCGTCCCCTCCAAGTTCTTGCTGCGTGCCGCCGAAGTCGCTCATCTGGCTGCGACGCGC
CGCTATCGCGGGATCCGCAGCCAACTCGAGGCAGTCGATCTGGCAGCCCATGTCGCGCAGAAGCAGGACCTGATCGCCGC
ACTGCGGCGCGAAAAGTACGAGGAACTCGTCGACTACTACGGTTGGGAACTCCTACACGGTACGGCCCGTTTCGTCGATC
CGCAGACAATCGCTGTCGGCGACCGCCTGATCCGCGCACGTGCCTTTCTGATCGCCACCGGGGCCAGTCCGGCTCTCCCG
CCGCTGCCTGGCCTCGCCGAGACGCCGTTCTTGACGAGCACGAGCGCGCTCGACCTGGAGCGCGTACCGACCTCGCTGCT
CGTGCTCGGTGCTGGGTACGTGGCCCTGGAACTCGGCCAAGCGTTCCAGCGGCTCGGCAGTCGCGTCACTCTCGTACAGC
GGCGAGCTCGGCTCTTGCCAGAAATCGAACTGAAGCTGGCCGAGCAACTCCAGCAGGCGCTCGCTGCGGAAGGGATCCGG
TTCCGCCTCGGCGTGCTCCCGCAGCGGGTCGAGCGCGTTCCTGGCGGCGTACGCCTCATCGTGCAGACCAGTGATGGTCG
CGAAGAAGCGCTCGAGGCTGAAACGCTGCTCGTGGCGACGGGACGCCGGCCGAACGTGGACGACCTCGATCTCGCTGCCG
CCGGTGTGGAACTCGACGCTCGGGGAGCCCCCAGACTCGATCCGACGCTCCGGACGACGAATCCCCGCATCTACGCCGCG
GGTGACGTGACGCTCGGCCCGCAGTTCGTGTATGTGGCAGCCGCGCAGGGCCGCCTGGCAGCTCGCAACGCGCTCCTCGG
AGAGCAGCAAATCCTGCGCCTCGATGCCGTACCAGCCGTCATCTTCACGCAGCCCCAGCTGGCCAGCGTCGGGCTGACCC
GAGCAGATGCCGAGGCGCGCGGGCATCGGGTCGTGACCGGCTTCGCTCCCGCGTCCGTGATCGCCCGCGAACGGGTCGAG
CTGCAACCGTTCGGCGGGGTGTTCCTGGTCGCTGATGCCGAGAGCGGGCGCATACTGGGTGTTCAGGCACTCGCCAGCGC
TGCCGGGGAACTGATCGAAGCAGCGACTCTGGCTGTCCATGCTGGGCTGACGCTCGCCGACCTCCGCGAGCATCTCGCCC
CCTACCTCACGGTTGGAGAGGGGCTGCGCTTAGCTGCGCTGGCTGTCGAGCAGGATCCCGCGCGCCTCTCATGTTGTGCG
TGA

Upstream 100 bases:

>100_bases
TGAGCCGATCGACCTGCCGTGCTCGAGCTGCGTTGCGGACCGAGCAGGCTGGCCTCGGCGAGTTCGCGCCCTGTGCCGAC
ATCGCGAAGGAGGAAGGACG

Downstream 100 bases:

>100_bases
GGAAGCGACGCATCATCGCCCGCCCACTCGGGTCGTTTCCCGGGCCGAGCACTGGCAACTGCGCACGAGTGGAAGCGCAT
GCTCGGGCATCGCTGTCGCA

Product: mercuric reductase

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MTPANHESALVTYELDVEGMTCRDCERHVETAVEASGGIEPRADWRRRRVRFAAPPTFDPQPVFAAIRAASGQLHRYEPG
QLRRLAPVQLDDESPDADADLVIIGGGSAAFAAAIEAHQLGARVIMIERGTLGGTCVNIGCVPSKFLLRAAEVAHLAATR
RYRGIRSQLEAVDLAAHVAQKQDLIAALRREKYEELVDYYGWELLHGTARFVDPQTIAVGDRLIRARAFLIATGASPALP
PLPGLAETPFLTSTSALDLERVPTSLLVLGAGYVALELGQAFQRLGSRVTLVQRRARLLPEIELKLAEQLQQALAAEGIR
FRLGVLPQRVERVPGGVRLIVQTSDGREEALEAETLLVATGRRPNVDDLDLAAAGVELDARGAPRLDPTLRTTNPRIYAA
GDVTLGPQFVYVAAAQGRLAARNALLGEQQILRLDAVPAVIFTQPQLASVGLTRADAEARGHRVVTGFAPASVIARERVE
LQPFGGVFLVADAESGRILGVQALASAAGELIEAATLAVHAGLTLADLREHLAPYLTVGEGLRLAALAVEQDPARLSCCA

Sequences:

>Translated_560_residues
MTPANHESALVTYELDVEGMTCRDCERHVETAVEASGGIEPRADWRRRRVRFAAPPTFDPQPVFAAIRAASGQLHRYEPG
QLRRLAPVQLDDESPDADADLVIIGGGSAAFAAAIEAHQLGARVIMIERGTLGGTCVNIGCVPSKFLLRAAEVAHLAATR
RYRGIRSQLEAVDLAAHVAQKQDLIAALRREKYEELVDYYGWELLHGTARFVDPQTIAVGDRLIRARAFLIATGASPALP
PLPGLAETPFLTSTSALDLERVPTSLLVLGAGYVALELGQAFQRLGSRVTLVQRRARLLPEIELKLAEQLQQALAAEGIR
FRLGVLPQRVERVPGGVRLIVQTSDGREEALEAETLLVATGRRPNVDDLDLAAAGVELDARGAPRLDPTLRTTNPRIYAA
GDVTLGPQFVYVAAAQGRLAARNALLGEQQILRLDAVPAVIFTQPQLASVGLTRADAEARGHRVVTGFAPASVIARERVE
LQPFGGVFLVADAESGRILGVQALASAAGELIEAATLAVHAGLTLADLREHLAPYLTVGEGLRLAALAVEQDPARLSCCA
>Mature_559_residues
TPANHESALVTYELDVEGMTCRDCERHVETAVEASGGIEPRADWRRRRVRFAAPPTFDPQPVFAAIRAASGQLHRYEPGQ
LRRLAPVQLDDESPDADADLVIIGGGSAAFAAAIEAHQLGARVIMIERGTLGGTCVNIGCVPSKFLLRAAEVAHLAATRR
YRGIRSQLEAVDLAAHVAQKQDLIAALRREKYEELVDYYGWELLHGTARFVDPQTIAVGDRLIRARAFLIATGASPALPP
LPGLAETPFLTSTSALDLERVPTSLLVLGAGYVALELGQAFQRLGSRVTLVQRRARLLPEIELKLAEQLQQALAAEGIRF
RLGVLPQRVERVPGGVRLIVQTSDGREEALEAETLLVATGRRPNVDDLDLAAAGVELDARGAPRLDPTLRTTNPRIYAAG
DVTLGPQFVYVAAAQGRLAARNALLGEQQILRLDAVPAVIFTQPQLASVGLTRADAEARGHRVVTGFAPASVIARERVEL
QPFGGVFLVADAESGRILGVQALASAAGELIEAATLAVHAGLTLADLREHLAPYLTVGEGLRLAALAVEQDPARLSCCA

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 HMA domains [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=494, Percent_Identity=31.1740890688259, Blast_Score=228, Evalue=2e-59,
Organism=Homo sapiens, GI50301238, Length=459, Percent_Identity=28.9760348583878, Blast_Score=154, Evalue=3e-37,
Organism=Homo sapiens, GI22035672, Length=464, Percent_Identity=30.3879310344828, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI148277071, Length=505, Percent_Identity=28.9108910891089, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI148277065, Length=505, Percent_Identity=28.9108910891089, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI33519430, Length=473, Percent_Identity=28.9640591966173, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI33519428, Length=473, Percent_Identity=28.9640591966173, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI33519426, Length=473, Percent_Identity=28.9640591966173, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI291045266, Length=499, Percent_Identity=27.2545090180361, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI291045268, Length=494, Percent_Identity=26.5182186234818, Blast_Score=109, Evalue=7e-24,
Organism=Escherichia coli, GI1786307, Length=467, Percent_Identity=30.1927194860814, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI87081717, Length=451, Percent_Identity=30.3769401330377, Blast_Score=188, Evalue=7e-49,
Organism=Escherichia coli, GI87082354, Length=464, Percent_Identity=29.0948275862069, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1789915, Length=436, Percent_Identity=29.3577981651376, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI1789065, Length=235, Percent_Identity=31.4893617021277, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=481, Percent_Identity=32.8482328482328, Blast_Score=227, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17557007, Length=497, Percent_Identity=28.7726358148893, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71982272, Length=511, Percent_Identity=28.7671232876712, Blast_Score=151, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI71983429, Length=353, Percent_Identity=30.8781869688385, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI71983419, Length=353, Percent_Identity=30.8781869688385, Blast_Score=142, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6321091, Length=476, Percent_Identity=31.5126050420168, Blast_Score=208, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6325240, Length=478, Percent_Identity=28.6610878661088, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6325166, Length=460, Percent_Identity=25.6521739130435, Blast_Score=131, Evalue=3e-31,
Organism=Drosophila melanogaster, GI21358499, Length=475, Percent_Identity=31.5789473684211, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24640549, Length=510, Percent_Identity=31.9607843137255, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24640551, Length=506, Percent_Identity=31.4229249011858, Blast_Score=179, Evalue=5e-45,
Organism=Drosophila melanogaster, GI24640553, Length=497, Percent_Identity=31.9919517102616, Blast_Score=178, Evalue=8e-45,
Organism=Drosophila melanogaster, GI17737741, Length=504, Percent_Identity=30.3571428571429, Blast_Score=165, Evalue=6e-41,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 59917; Mature: 59786

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50846 HMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPANHESALVTYELDVEGMTCRDCERHVETAVEASGGIEPRADWRRRRVRFAAPPTFDP
CCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCEEECCCCCCCC
QPVFAAIRAASGQLHRYEPGQLRRLAPVQLDDESPDADADLVIIGGGSAAFAAAIEAHQL
CHHHHHHHHCCCCCCCCCCCHHCEECCEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHC
GARVIMIERGTLGGTCVNIGCVPSKFLLRAAEVAHLAATRRYRGIRSQLEAVDLAAHVAQ
CCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQDLIAALRREKYEELVDYYGWELLHGTARFVDPQTIAVGDRLIRARAFLIATGASPALP
HHHHHHHHHHHHHHHHHHHHCHHHCCCHHHHCCCCEEHHHHHHHHHEEEEEEECCCCCCC
PLPGLAETPFLTSTSALDLERVPTSLLVLGAGYVALELGQAFQRLGSRVTLVQRRARLLP
CCCCCCCCCCCCCCCCCCHHHCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHCC
EIELKLAEQLQQALAAEGIRFRLGVLPQRVERVPGGVRLIVQTSDGREEALEAETLLVAT
HHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCEEEEEECCCCHHHHHHHCEEEEEC
GRRPNVDDLDLAAAGVELDARGAPRLDPTLRTTNPRIYAAGDVTLGPQFVYVAAAQGRLA
CCCCCCCCCHHHEECEEEECCCCCCCCCCEECCCCEEEEECCCEECCCEEEEEECCCCHH
ARNALLGEQQILRLDAVPAVIFTQPQLASVGLTRADAEARGHRVVTGFAPASVIARERVE
HHHHHCCHHHHHHHHCCCEEEECCCCHHHCCCEECCCCCCCCEEEEECCCHHHHHHHCEE
LQPFGGVFLVADAESGRILGVQALASAAGELIEAATLAVHAGLTLADLREHLAPYLTVGE
ECCCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEECCC
GLRLAALAVEQDPARLSCCA
CCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
TPANHESALVTYELDVEGMTCRDCERHVETAVEASGGIEPRADWRRRRVRFAAPPTFDP
CCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCEEECCCCCCCC
QPVFAAIRAASGQLHRYEPGQLRRLAPVQLDDESPDADADLVIIGGGSAAFAAAIEAHQL
CHHHHHHHHCCCCCCCCCCCHHCEECCEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHC
GARVIMIERGTLGGTCVNIGCVPSKFLLRAAEVAHLAATRRYRGIRSQLEAVDLAAHVAQ
CCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQDLIAALRREKYEELVDYYGWELLHGTARFVDPQTIAVGDRLIRARAFLIATGASPALP
HHHHHHHHHHHHHHHHHHHHCHHHCCCHHHHCCCCEEHHHHHHHHHEEEEEEECCCCCCC
PLPGLAETPFLTSTSALDLERVPTSLLVLGAGYVALELGQAFQRLGSRVTLVQRRARLLP
CCCCCCCCCCCCCCCCCCHHHCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHCC
EIELKLAEQLQQALAAEGIRFRLGVLPQRVERVPGGVRLIVQTSDGREEALEAETLLVAT
HHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCEEEEEECCCCHHHHHHHCEEEEEC
GRRPNVDDLDLAAAGVELDARGAPRLDPTLRTTNPRIYAAGDVTLGPQFVYVAAAQGRLA
CCCCCCCCCHHHEECEEEECCCCCCCCCCEECCCCEEEEECCCEECCCEEEEEECCCCHH
ARNALLGEQQILRLDAVPAVIFTQPQLASVGLTRADAEARGHRVVTGFAPASVIARERVE
HHHHHCCHHHHHHHHCCCEEEECCCCHHHCCCEECCCCCCCCEEEEECCCHHHHHHHCEE
LQPFGGVFLVADAESGRILGVQALASAAGELIEAATLAVHAGLTLADLREHLAPYLTVGE
ECCCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEECCC
GLRLAALAVEQDPARLSCCA
CCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2536669; 10559175; 2067577 [H]