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The map label for this gene is hcaF [H]

Identifier: 221635746

GI number: 221635746

Start: 327674

End: 328228

Strand: Reverse

Name: hcaF [H]

Synonym: trd_A0340

Alternate gene names: 221635746

Gene position: 328228-327674 (Counterclockwise)

Preceding gene: 221635747

Following gene: 221635745

Centisome position: 35.76

GC content: 54.95

Gene sequence:

>555_bases
ATGCTTCTGCAGGACATTCCGTTGCCAGCAATCGATCTGGATTACATCAAGCTTTATCATGAACTGTACCAGTTCCTTTC
GTATGAAGCCCTTCTTCTGTCGGAACGCAAATATGATGATTGGCTGGCCCTGTTTACGGATGATGCCGTGTACGAGGTGC
CTATTCGCGTGACGAGAGAACGCGGAGCCGCGTGGGAACTCTCACCGACTGGTCGACTCTTCGACGACACCAAGGAAACG
CTCGCTGTCCGTATTGCACGACTCAACACCGAATATGCGTGGGCCGAAGACCCACCGTCGCGAGTCCGATACTATCTCTC
CAATCTCCTGATCACGTCCCGAAGCAATGACCTCCCAGAAAACCAGCAGGAGGTCACTGCCCGATACAACGTCCTGGTGA
TCCGAACCCGCGGCGAGTTACCGGATATCGAGTTCGTCTCCACCCACCGCCAAGATCGGCTGCGGCACACCCAGGATGGC
TGGAGGATCGCTCATCGCACCATCATCATCGATCACAGTGTCCTTCAGGTGCGCAACCTTTCCTTCTTCCTGTGA

Upstream 100 bases:

>100_bases
CGTCGATCCGACGAAGGTGCCACCAGCGTTCTACCCTACCAAGCACGCGAGCGCAGCTGAATGACGTATCGGCTAGACCA
GGGCGCGAAAGGAGCGAACC

Downstream 100 bases:

>100_bases
TGACGAGACGGGAGGGGCTCAGATGAGCCACGAGATCCATGACTCACATGACCGCATCACTCACGGTGTCGAAGTAGCGA
GCGAGACGATCGTTATCAAG

Product: 3-phenylpropionate dioxygenase subunit beta (Digoxigenin subunit beta)

Products: NA

Alternate protein names: Digoxigenin subunit beta [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MLLQDIPLPAIDLDYIKLYHELYQFLSYEALLLSERKYDDWLALFTDDAVYEVPIRVTRERGAAWELSPTGRLFDDTKET
LAVRIARLNTEYAWAEDPPSRVRYYLSNLLITSRSNDLPENQQEVTARYNVLVIRTRGELPDIEFVSTHRQDRLRHTQDG
WRIAHRTIIIDHSVLQVRNLSFFL

Sequences:

>Translated_184_residues
MLLQDIPLPAIDLDYIKLYHELYQFLSYEALLLSERKYDDWLALFTDDAVYEVPIRVTRERGAAWELSPTGRLFDDTKET
LAVRIARLNTEYAWAEDPPSRVRYYLSNLLITSRSNDLPENQQEVTARYNVLVIRTRGELPDIEFVSTHRQDRLRHTQDG
WRIAHRTIIIDHSVLQVRNLSFFL
>Mature_184_residues
MLLQDIPLPAIDLDYIKLYHELYQFLSYEALLLSERKYDDWLALFTDDAVYEVPIRVTRERGAAWELSPTGRLFDDTKET
LAVRIARLNTEYAWAEDPPSRVRYYLSNLLITSRSNDLPENQQEVTARYNVLVIRTRGELPDIEFVSTHRQDRLRHTQDG
WRIAHRTIIIDHSVLQVRNLSFFL

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively [H]

COG id: COG5517

COG function: function code Q; Small subunit of phenylpropionate dioxygenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family [H]

Homologues:

Organism=Escherichia coli, GI1788889, Length=173, Percent_Identity=38.150289017341, Blast_Score=100, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000391 [H]

Pfam domain/function: PF00866 Ring_hydroxyl_B [H]

EC number: =1.14.12.19 [H]

Molecular weight: Translated: 21766; Mature: 21766

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLQDIPLPAIDLDYIKLYHELYQFLSYEALLLSERKYDDWLALFTDDAVYEVPIRVTRE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEECEEEEEC
RGAAWELSPTGRLFDDTKETLAVRIARLNTEYAWAEDPPSRVRYYLSNLLITSRSNDLPE
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHEEECCCCCCCC
NQQEVTARYNVLVIRTRGELPDIEFVSTHRQDRLRHTQDGWRIAHRTIIIDHSVLQVRNL
CHHHHEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECHHHHHCCC
SFFL
EECC
>Mature Secondary Structure
MLLQDIPLPAIDLDYIKLYHELYQFLSYEALLLSERKYDDWLALFTDDAVYEVPIRVTRE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEECEEEEEC
RGAAWELSPTGRLFDDTKETLAVRIARLNTEYAWAEDPPSRVRYYLSNLLITSRSNDLPE
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHEEECCCCCCCC
NQQEVTARYNVLVIRTRGELPDIEFVSTHRQDRLRHTQDGWRIAHRTIIIDHSVLQVRNL
CHHHHEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECHHHHHCCC
SFFL
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]