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The map label for this gene is pyrE [H]

Identifier: 221635659

GI number: 221635659

Start: 238711

End: 239349

Strand: Reverse

Name: pyrE [H]

Synonym: trd_A0253

Alternate gene names: 221635659

Gene position: 239349-238711 (Counterclockwise)

Preceding gene: 221635660

Following gene: 221635658

Centisome position: 26.08

GC content: 62.28

Gene sequence:

>639_bases
GTGCTCTTCGGTACCGAAACCAATCTCGACATCGCGCGCGCCTTGTTCGAGCTCGGCGGCGTCCTGTTCGGCGAGTTCGA
TCTCGGTCCCACAGCTGGTCGTTCTCCGATCTACATCAACCCACGCGTTTTGATCAGCGAACCGGCGCTGCTGCGTCGGA
TCGCCCACCTGATTCACCACGAAATTCAAGCTGACCAGGCCCGGCGACGACCACGTCTCGCCTCGTTCGCAGCCGTCGCT
GGCGTCCCCATGGGCGGTCTTCACCTGGCCACTGCCTACGCCCTGGTCAGCGATACCCCGCTCATCTACGTGCGTCCCGA
CGCTCAGGAACCAGCGTTGGAAGGTCGCATCGTCCCTGGGCAGACAGTGCTGGTGATCGACGACCTGATGACCGGGGGCA
CCAGCTTGCTCCGCACCGCTCGCATTCTGGAAGAAGCTGGTCTCACCGTGCGCGACTTCATCGTGCTGATCGATCGCGAG
CAAGGGGGAGCCGAGCGTCTCCATGCTCACGGGTATCATGTCATGCCGATCTTGCGGTTGCGCACGATGCTCACCTACTA
CTTCGAGAGCGGCATGATCGATAGCCAACGCTATCGCACCGCGATGGACTATCTCGAGCGGACCAACCGTCGGGGGTGA

Upstream 100 bases:

>100_bases
CGGACGACAGGCACACGGCACCGAGCCGATCATGCCGATTCGACGCAGGTCGACGCGGCCGGTGGAACTGAGACGAGATC
TCGGCGGAGGTGAGGGAGAC

Downstream 100 bases:

>100_bases
GGAGGAGGCCGCCGATGCCAGCGCGAGGTCGGCTCGCGGGACAGGACGTGCTCGTCTTCGATCTCGAACAGCCGCGCACC
CCGGAGATGCCGATCTATCC

Product: uridine 5'-monophosphate synthase (UMP synthase) (Fragment)

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MLFGTETNLDIARALFELGGVLFGEFDLGPTAGRSPIYINPRVLISEPALLRRIAHLIHHEIQADQARRRPRLASFAAVA
GVPMGGLHLATAYALVSDTPLIYVRPDAQEPALEGRIVPGQTVLVIDDLMTGGTSLLRTARILEEAGLTVRDFIVLIDRE
QGGAERLHAHGYHVMPILRLRTMLTYYFESGMIDSQRYRTAMDYLERTNRRG

Sequences:

>Translated_212_residues
MLFGTETNLDIARALFELGGVLFGEFDLGPTAGRSPIYINPRVLISEPALLRRIAHLIHHEIQADQARRRPRLASFAAVA
GVPMGGLHLATAYALVSDTPLIYVRPDAQEPALEGRIVPGQTVLVIDDLMTGGTSLLRTARILEEAGLTVRDFIVLIDRE
QGGAERLHAHGYHVMPILRLRTMLTYYFESGMIDSQRYRTAMDYLERTNRRG
>Mature_212_residues
MLFGTETNLDIARALFELGGVLFGEFDLGPTAGRSPIYINPRVLISEPALLRRIAHLIHHEIQADQARRRPRLASFAAVA
GVPMGGLHLATAYALVSDTPLIYVRPDAQEPALEGRIVPGQTVLVIDDLMTGGTSLLRTARILEEAGLTVRDFIVLIDRE
QGGAERLHAHGYHVMPILRLRTMLTYYFESGMIDSQRYRTAMDYLERTNRRG

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=186, Percent_Identity=34.9462365591398, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17554964, Length=194, Percent_Identity=28.3505154639175, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17508631, Length=179, Percent_Identity=30.1675977653631, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17933654, Length=199, Percent_Identity=30.6532663316583, Blast_Score=90, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 23606; Mature: 23606

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFGTETNLDIARALFELGGVLFGEFDLGPTAGRSPIYINPRVLISEPALLRRIAHLIHH
CCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCEEECCEEEECCCHHHHHHHHHHHH
EIQADQARRRPRLASFAAVAGVPMGGLHLATAYALVSDTPLIYVRPDAQEPALEGRIVPG
HHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEECCC
QTVLVIDDLMTGGTSLLRTARILEEAGLTVRDFIVLIDREQGGAERLHAHGYHVMPILRL
CEEEEEECHHHCCHHHHHHHHHHHHHCCEEEHEEEEEECCCCCHHHHHHCCCHHHHHHHH
RTMLTYYFESGMIDSQRYRTAMDYLERTNRRG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLFGTETNLDIARALFELGGVLFGEFDLGPTAGRSPIYINPRVLISEPALLRRIAHLIHH
CCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCEEECCEEEECCCHHHHHHHHHHHH
EIQADQARRRPRLASFAAVAGVPMGGLHLATAYALVSDTPLIYVRPDAQEPALEGRIVPG
HHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEECCC
QTVLVIDDLMTGGTSLLRTARILEEAGLTVRDFIVLIDREQGGAERLHAHGYHVMPILRL
CEEEEEECHHHCCHHHHHHHHHHHHHCCEEEHEEEEEECCCCCHHHHHHCCCHHHHHHHH
RTMLTYYFESGMIDSQRYRTAMDYLERTNRRG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12682364 [H]