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The map label for this gene is yfjR [H]

Identifier: 221635420

GI number: 221635420

Start: 12942

End: 13826

Strand: Reverse

Name: yfjR [H]

Synonym: trd_A0014

Alternate gene names: 221635420

Gene position: 13826-12942 (Counterclockwise)

Preceding gene: 221635421

Following gene: 221635418

Centisome position: 1.51

GC content: 67.68

Gene sequence:

>885_bases
ATGGACATCGGTTTTATCGGGCTGGGCAACATGGGACGCGGCATGGTCCAGTCGCTCTTGCGAGCAGGCCACCGCGTCGT
CGTCTGGAACCGCACACCGGAGCGAGCGGACGAGCTGGTTGCACTCGGCGCCGAGCGTGCTGCCACCCCTTTCGAGGCCG
CGGAAGTGGGCGTCGTCTTCACCATGCTGGCCGACGATGCTGCCACCGAGGCAGTCGTCGCCGGACCGCATGGCATCCTG
GCCGGACTCCCGCGCGACGGCATCCATGTCGCGTCCAGCACGCTCAGCGTCCGCTATTCGGCTGAGCTGGCCGAGCGGCA
CCGCACGCGCGGGCAGCACTATGTCGCTGCCCCCGTCTTCGGTCGCCCCGATGCTGCCGCGCAGGGCGCGCTCCGGCTGG
TGATCGCTGGGCAGCCCGGTGCGATCGCACGCTTGCAGCCGGCCTTCGCCGCGCTCGGTTCCCAGCAGTTCGTCATCGGC
GAGCGGCCGGAGCACGCGCACGCCGTCAAGATCGCTGGTAATTTCCTCATTCTCTCGCTGGTGGAGCTCCTGAGCGAGGC
CTTCGTTTTGGCCGACAAGGCGGGCGTATCCCGCGAGCGTTTCTTCGAGGTCGCCAGCGCTGTCTTTTCCTCGCCGCTCG
CCGATCGCTATGGTCGCGCGTTGCTCGAGCAGCGCTTCCAACCACCAGGTTTTCGCCTCACGCTCGGGCTCAAGGACATC
CGCTTGGCGCTCGAGTTGGCCGAAGCGACGCACACGCCGTTACCGATCGCCGACCTCGTCCATCAGCATGCGCTGGAGGC
GATGGCCCGTGGCCAGGCCGAACTCGACTTCACCTCGCTCCTGCGCGTCGTGGAAGCGCATGCTGGGCTGCATCGACCCG
AGTAG

Upstream 100 bases:

>100_bases
CCAAGTTCCACGCGTCTTCCCCCTGTCCTCTCGCCGACTGGTGACCGCCCGGGTACGCTACCACCAGGTGAGCGAGTCGT
TGCGGCGAGGAGGTCGGGCG

Downstream 100 bases:

>100_bases
CGCATCGGCAGACGTGGAGCTTTCCCGGCGGGGCTGATCTGCTCGTGCCCACAGCACCGGCGAGCGGTCGCTGGCCACGC
GCGCTGGTAACCGGCTTGCC

Product: 3-hydroxyisobutyrate dehydrogenase family protein

Products: (R)-Glycerate; Nicotinamide adenine dinucleotide [C]

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MDIGFIGLGNMGRGMVQSLLRAGHRVVVWNRTPERADELVALGAERAATPFEAAEVGVVFTMLADDAATEAVVAGPHGIL
AGLPRDGIHVASSTLSVRYSAELAERHRTRGQHYVAAPVFGRPDAAAQGALRLVIAGQPGAIARLQPAFAALGSQQFVIG
ERPEHAHAVKIAGNFLILSLVELLSEAFVLADKAGVSRERFFEVASAVFSSPLADRYGRALLEQRFQPPGFRLTLGLKDI
RLALELAEATHTPLPIADLVHQHALEAMARGQAELDFTSLLRVVEAHAGLHRPE

Sequences:

>Translated_294_residues
MDIGFIGLGNMGRGMVQSLLRAGHRVVVWNRTPERADELVALGAERAATPFEAAEVGVVFTMLADDAATEAVVAGPHGIL
AGLPRDGIHVASSTLSVRYSAELAERHRTRGQHYVAAPVFGRPDAAAQGALRLVIAGQPGAIARLQPAFAALGSQQFVIG
ERPEHAHAVKIAGNFLILSLVELLSEAFVLADKAGVSRERFFEVASAVFSSPLADRYGRALLEQRFQPPGFRLTLGLKDI
RLALELAEATHTPLPIADLVHQHALEAMARGQAELDFTSLLRVVEAHAGLHRPE
>Mature_294_residues
MDIGFIGLGNMGRGMVQSLLRAGHRVVVWNRTPERADELVALGAERAATPFEAAEVGVVFTMLADDAATEAVVAGPHGIL
AGLPRDGIHVASSTLSVRYSAELAERHRTRGQHYVAAPVFGRPDAAAQGALRLVIAGQPGAIARLQPAFAALGSQQFVIG
ERPEHAHAVKIAGNFLILSLVELLSEAFVLADKAGVSRERFFEVASAVFSSPLADRYGRALLEQRFQPPGFRLTLGLKDI
RLALELAEATHTPLPIADLVHQHALEAMARGQAELDFTSLLRVVEAHAGLHRPE

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=282, Percent_Identity=31.9148936170213, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI23308751, Length=298, Percent_Identity=26.510067114094, Blast_Score=101, Evalue=7e-22,
Organism=Escherichia coli, GI1786719, Length=290, Percent_Identity=30.6896551724138, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI145693186, Length=278, Percent_Identity=29.8561151079137, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1790315, Length=296, Percent_Identity=30.0675675675676, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789092, Length=287, Percent_Identity=25.0871080139373, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17557316, Length=271, Percent_Identity=26.9372693726937, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28574115, Length=281, Percent_Identity=26.6903914590747, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24655230, Length=293, Percent_Identity=26.6211604095563, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19922568, Length=293, Percent_Identity=26.6211604095563, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.60 [C]

Molecular weight: Translated: 31437; Mature: 31437

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIGFIGLGNMGRGMVQSLLRAGHRVVVWNRTPERADELVALGAERAATPFEAAEVGVVF
CCEEEEECCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCHHCCCCCHHHHHHEEE
TMLADDAATEAVVAGPHGILAGLPRDGIHVASSTLSVRYSAELAERHRTRGQHYVAAPVF
EEHHCCHHCCEEEECCCHHEECCCCCCCEEHHHHEEEEEHHHHHHHHHHCCCEEEEECCC
GRPDAAAQGALRLVIAGQPGAIARLQPAFAALGSQQFVIGERPEHAHAVKIAGNFLILSL
CCCCHHCCCCEEEEEECCCCCCHHHCHHHHHCCCCEEEECCCCCCCEEEEEECCHHHHHH
VELLSEAFVLADKAGVSRERFFEVASAVFSSPLADRYGRALLEQRFQPPGFRLTLGLKDI
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECHHHH
RLALELAEATHTPLPIADLVHQHALEAMARGQAELDFTSLLRVVEAHAGLHRPE
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDIGFIGLGNMGRGMVQSLLRAGHRVVVWNRTPERADELVALGAERAATPFEAAEVGVVF
CCEEEEECCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCHHCCCCCHHHHHHEEE
TMLADDAATEAVVAGPHGILAGLPRDGIHVASSTLSVRYSAELAERHRTRGQHYVAAPVF
EEHHCCHHCCEEEECCCHHEECCCCCCCEEHHHHEEEEEHHHHHHHHHHCCCEEEEECCC
GRPDAAAQGALRLVIAGQPGAIARLQPAFAALGSQQFVIGERPEHAHAVKIAGNFLILSL
CCCCHHCCCCEEEEEECCCCCCHHHCHHHHHCCCCEEEECCCCCCCEEEEEECCHHHHHH
VELLSEAFVLADKAGVSRERFFEVASAVFSSPLADRYGRALLEQRFQPPGFRLTLGLKDI
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECHHHH
RLALELAEATHTPLPIADLVHQHALEAMARGQAELDFTSLLRVVEAHAGLHRPE
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Hydroxy-3-oxopropanoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Specific reaction: 2-Hydroxy-3-oxopropanoate + H+ + Nicotinamide adenine dinucleotide - reduced --> (R)-Glycerate + Nicotinamide adenine dinucleotide [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]