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The map label for this gene is fdxH [H]

Identifier: 221633945

GI number: 221633945

Start: 1990524

End: 1991372

Strand: Reverse

Name: fdxH [H]

Synonym: trd_1973

Alternate gene names: 221633945

Gene position: 1991372-1990524 (Counterclockwise)

Preceding gene: 221633946

Following gene: 221633944

Centisome position: 99.42

GC content: 64.31

Gene sequence:

>849_bases
ATGAGCAGCACGGTGCCGGTCGGGCGGACGCGCGCCCGGCCAGGCGCCTTCGGCTTCCTCACCGATACCACCCTCTGCAT
CGGCTGTAAAGCGTGCGAGGTCGCCTGCAAGCAGTGGAACCAGCTGCCGATGGACGATTTCGGCTTCACCGGCATGAGTT
ACGACAATACCGGCGATCTCGGCGCGACCACCTGGCGTCATGTCGCCTTCATCGAGCGGATCAGCGATCGGGAAGGGCGA
CGTCCAACGACCCTCGCGCCGTTCCAGAGCAACTGGCTCATGCTGAGCGACGTCTGCAAGCATTGTGTCCACGCCGGTTG
CATGGAGGCATGCCCGACTGGCGCCATCATCCGCACCGAGTTCGATACGGTCGTGATCCAGCAGGATATCTGCAACGGCT
GCGGCTACTGTGTTCCGGCCTGCCCCTTCGGCGTCCCCGCCCTCAGTCCATTCGACGGCAAGGCGCACAAGTGCACGCTC
TGCTATGACCGGCTCAAGGATGGCCTGGAACCAGCCTGCGCCAAGGCCTGCCCCACCGATGCCATTCAGTTCGGTCCGGT
GCATGCTTTGATGGACCGGGCACGAGCGCGGGTCAGCGAACTGCGCGCCCAAGGTGTGGAAGCTCGCATTTACGGTGACC
AGGCACTCGGTGGTACCGGCGGCATCGGTGGGCTCAACGCCTTCTTCATCCTGACTGCACCGCCGGAAGTCTATAACCTG
CCGGCCAAACCCGAGCTTCCCCAGCGGCGCGTGTTGCCCGCGTTTCTCACCACCGCGCTGGCCGCACTCGCACTCGGCAT
CGCCACTCTGTTCTCCTTCCGACGAGTGGGAAGGATGCAGCATGGCTGA

Upstream 100 bases:

>100_bases
AGCTGGCTGCGCTGGTCGAGGATCCCAACTCGCTCATCCACGAGGCCAAGTCCTTTACCTGCACGGTTCGCAAAGGACGA
CTACGCGAAGGGACTGAACC

Downstream 100 bases:

>100_bases
ACTCCGGTCCTGGCGACACCGACGCGCACCTGGCGAGCCTATCGGTGCCGCGACACGTGAAAGCTATTACGGACTCCCCG
TCATCCATGGCCCGCATTGG

Product: putative [Ni/Fe] hydrogenase, iron-sulfur cluster-binding subunit

Products: CO2; NADH

Alternate protein names: Formate dehydrogenase subunit beta; FDH subunit beta [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSSTVPVGRTRARPGAFGFLTDTTLCIGCKACEVACKQWNQLPMDDFGFTGMSYDNTGDLGATTWRHVAFIERISDREGR
RPTTLAPFQSNWLMLSDVCKHCVHAGCMEACPTGAIIRTEFDTVVIQQDICNGCGYCVPACPFGVPALSPFDGKAHKCTL
CYDRLKDGLEPACAKACPTDAIQFGPVHALMDRARARVSELRAQGVEARIYGDQALGGTGGIGGLNAFFILTAPPEVYNL
PAKPELPQRRVLPAFLTTALAALALGIATLFSFRRVGRMQHG

Sequences:

>Translated_282_residues
MSSTVPVGRTRARPGAFGFLTDTTLCIGCKACEVACKQWNQLPMDDFGFTGMSYDNTGDLGATTWRHVAFIERISDREGR
RPTTLAPFQSNWLMLSDVCKHCVHAGCMEACPTGAIIRTEFDTVVIQQDICNGCGYCVPACPFGVPALSPFDGKAHKCTL
CYDRLKDGLEPACAKACPTDAIQFGPVHALMDRARARVSELRAQGVEARIYGDQALGGTGGIGGLNAFFILTAPPEVYNL
PAKPELPQRRVLPAFLTTALAALALGIATLFSFRRVGRMQHG
>Mature_281_residues
SSTVPVGRTRARPGAFGFLTDTTLCIGCKACEVACKQWNQLPMDDFGFTGMSYDNTGDLGATTWRHVAFIERISDREGRR
PTTLAPFQSNWLMLSDVCKHCVHAGCMEACPTGAIIRTEFDTVVIQQDICNGCGYCVPACPFGVPALSPFDGKAHKCTLC
YDRLKDGLEPACAKACPTDAIQFGPVHALMDRARARVSELRAQGVEARIYGDQALGGTGGIGGLNAFFILTAPPEVYNLP
AKPELPQRRVLPAFLTTALAALALGIATLFSFRRVGRMQHG

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=283, Percent_Identity=37.4558303886926, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1790326, Length=279, Percent_Identity=36.9175627240143, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1787122, Length=177, Percent_Identity=32.7683615819209, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787872, Length=177, Percent_Identity=32.2033898305085, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1789370, Length=200, Percent_Identity=31, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI2367345, Length=190, Percent_Identity=31.5789473684211, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI226510944, Length=185, Percent_Identity=28.6486486486486, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788811, Length=186, Percent_Identity=31.1827956989247, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787740, Length=144, Percent_Identity=30.5555555555556, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787478, Length=92, Percent_Identity=35.8695652173913, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI2367245, Length=190, Percent_Identity=31.5789473684211, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789067, Length=201, Percent_Identity=30.8457711442786, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082179, Length=188, Percent_Identity=29.2553191489362, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 30408; Mature: 30277

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
5.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTVPVGRTRARPGAFGFLTDTTLCIGCKACEVACKQWNQLPMDDFGFTGMSYDNTGDL
CCCCCCCCCCCCCCCCEEEHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GATTWRHVAFIERISDREGRRPTTLAPFQSNWLMLSDVCKHCVHAGCMEACPTGAIIRTE
CCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCEEHHHHHHHHHHHHHHHHCCCCCEEEEC
FDTVVIQQDICNGCGYCVPACPFGVPALSPFDGKAHKCTLCYDRLKDGLEPACAKACPTD
CCHHEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHCCCCC
AIQFGPVHALMDRARARVSELRAQGVEARIYGDQALGGTGGIGGLNAFFILTAPPEVYNL
CHHHCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEEEECCHHHHCC
PAKPELPQRRVLPAFLTTALAALALGIATLFSFRRVGRMQHG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSTVPVGRTRARPGAFGFLTDTTLCIGCKACEVACKQWNQLPMDDFGFTGMSYDNTGDL
CCCCCCCCCCCCCCCEEEHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GATTWRHVAFIERISDREGRRPTTLAPFQSNWLMLSDVCKHCVHAGCMEACPTGAIIRTE
CCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCEEHHHHHHHHHHHHHHHHCCCCCEEEEC
FDTVVIQQDICNGCGYCVPACPFGVPALSPFDGKAHKCTLCYDRLKDGLEPACAKACPTD
CCHHEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHCCCCC
AIQFGPVHALMDRARARVSELRAQGVEARIYGDQALGGTGGIGGLNAFFILTAPPEVYNL
CHHHCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEEEECCHHHHCC
PAKPELPQRRVLPAFLTTALAALALGIATLFSFRRVGRMQHG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]