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The map label for this gene is yfnC [H]

Identifier: 221633468

GI number: 221633468

Start: 1472728

End: 1473951

Strand: Reverse

Name: yfnC [H]

Synonym: trd_1489

Alternate gene names: 221633468

Gene position: 1473951-1472728 (Counterclockwise)

Preceding gene: 221633474

Following gene: 221633466

Centisome position: 73.59

GC content: 66.58

Gene sequence:

>1224_bases
ATGGGGACGGAGGCAGCCGCTGCGCGCCCGCGAGCAGTCGGCACGCCAGCCATTTGGGACGCGCGCGGCGTGTTGCTCAT
GTCGTGGGCGCACTTCGCTCATGACCTCTATCCTTCGTTCCTCGGCGTCCTGGTTCCGGCGATCCAGGCGAAGCTCGGGG
TCTCGCTGTTCTGGGCCAGCGCCATGGTTCCTGCACAGCAGCTTCCTTCGATCGTGCAGCCGTTTCTCGGCTACGTGGCG
GACCGGACGAGCCGCCGCTGGTTCGTCGTGCTCTCGCCGGCAGTCGCGGCGCTCGCGCTCTCTTCCATCGGGATCGCACC
GCACTTCGCCGTCGCCCTCGTGCTCCTCCTCGTTTCGGGACTTGCCTCGGCTGCCTTCCATGCGCCGGCTGTGGCGTTGG
TCGGCGAGTTCGGTGGACAGCGGCTCGGTCGGGCTATGGCGATCTTCATGGCCGGTGGTGAGTTGGCGCGGACAGTCGCA
CCCATCCTGCTGACCGCAGTGATCGCACATTTCACCTTGGACGGGATGCCGCTCGTCATGCTCGTCGGCCTCGCCGCCTC
AGCGACGCTCTTCCTGCGACTCCGGACCGAGGCGAGCGACGCGATCGCGCGGCGGCACGCTGGCCAAGTTCAGTTGTGGC
GGCTCATCCGCGAGCGCCAGCGACCGTTCATCGGGCTCCTGGGATCGGTTGTGGCGAATACCGCAGCAGTGACACCCCCC
AGCTTCTTTCTGGTCGAATATCTTCTCCAGCGTGGTCGGAGCGAGTGGTACGGTGGACTGGCCCTGTCTGCCTTCTATGC
CAGTGGAATCATCGGCGGGCTGGTCGGGGGATCGCTCTCCGACTGGGTGGGACGACGCGGCGCGTTGCTCCTCTCCTCGC
TCGGGACCGCGCCGCTCCTCGCGCTCTACCTGGCTATCGAAAACGGGAGTTGGCTCATGCTGCCGCTGCTGGTTCTGATC
GGGGTTTTTGCAACACCGCCGCGCTCGGTGACGCTGGCACTGGCGAACGACATTGCCCCCGAAGCCCGTGGCCCGCTGTC
TGGCTTCACGCTCGCGACCAGTTTCGTTGCTCAAAGCATCATCGCGCTCGCCTTCGGTGCCGTGGCCGAGGCGATCGGTA
TCGAGCGAGCGTTCTGGCTGACGGTCGGCGCCTCGTTGGCCGGGGTACCGTTCGCGTTGCTGATCCCGGCCACGATGGGG
CGGTCCCCTTCGTCAACAGGATGA

Upstream 100 bases:

>100_bases
AATGGCGATGCCGCACGGGCAGCGAAGGGGCCTATACTGGTGCGACAGCTGTCGCGAGCCGATCTGCGGCTCCGGTCGAT
CCTTGGCGAGGAGGAAGGCG

Downstream 100 bases:

>100_bases
GACCGGACGGTGAGCCTCCAAGCACGTGACCCCACGGGGGCCGACGACCGCGCTGTGCACGGTTCCTGCCGGGAGATCGA
GGCGGTCACCTGGCCCCAAC

Product: putative efflux protein

Products: fosmidomycin [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MGTEAAAARPRAVGTPAIWDARGVLLMSWAHFAHDLYPSFLGVLVPAIQAKLGVSLFWASAMVPAQQLPSIVQPFLGYVA
DRTSRRWFVVLSPAVAALALSSIGIAPHFAVALVLLLVSGLASAAFHAPAVALVGEFGGQRLGRAMAIFMAGGELARTVA
PILLTAVIAHFTLDGMPLVMLVGLAASATLFLRLRTEASDAIARRHAGQVQLWRLIRERQRPFIGLLGSVVANTAAVTPP
SFFLVEYLLQRGRSEWYGGLALSAFYASGIIGGLVGGSLSDWVGRRGALLLSSLGTAPLLALYLAIENGSWLMLPLLVLI
GVFATPPRSVTLALANDIAPEARGPLSGFTLATSFVAQSIIALAFGAVAEAIGIERAFWLTVGASLAGVPFALLIPATMG
RSPSSTG

Sequences:

>Translated_407_residues
MGTEAAAARPRAVGTPAIWDARGVLLMSWAHFAHDLYPSFLGVLVPAIQAKLGVSLFWASAMVPAQQLPSIVQPFLGYVA
DRTSRRWFVVLSPAVAALALSSIGIAPHFAVALVLLLVSGLASAAFHAPAVALVGEFGGQRLGRAMAIFMAGGELARTVA
PILLTAVIAHFTLDGMPLVMLVGLAASATLFLRLRTEASDAIARRHAGQVQLWRLIRERQRPFIGLLGSVVANTAAVTPP
SFFLVEYLLQRGRSEWYGGLALSAFYASGIIGGLVGGSLSDWVGRRGALLLSSLGTAPLLALYLAIENGSWLMLPLLVLI
GVFATPPRSVTLALANDIAPEARGPLSGFTLATSFVAQSIIALAFGAVAEAIGIERAFWLTVGASLAGVPFALLIPATMG
RSPSSTG
>Mature_406_residues
GTEAAAARPRAVGTPAIWDARGVLLMSWAHFAHDLYPSFLGVLVPAIQAKLGVSLFWASAMVPAQQLPSIVQPFLGYVAD
RTSRRWFVVLSPAVAALALSSIGIAPHFAVALVLLLVSGLASAAFHAPAVALVGEFGGQRLGRAMAIFMAGGELARTVAP
ILLTAVIAHFTLDGMPLVMLVGLAASATLFLRLRTEASDAIARRHAGQVQLWRLIRERQRPFIGLLGSVVANTAAVTPPS
FFLVEYLLQRGRSEWYGGLALSAFYASGIIGGLVGGSLSDWVGRRGALLLSSLGTAPLLALYLAIENGSWLMLPLLVLIG
VFATPPRSVTLALANDIAPEARGPLSGFTLATSFVAQSIIALAFGAVAEAIGIERAFWLTVGASLAGVPFALLIPATMGR
SPSSTG

Specific function: Confers The Resistance Against Fosmidomycin. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786686, Length=365, Percent_Identity=23.8356164383562, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42450; Mature: 42318

Theoretical pI: Translated: 11.08; Mature: 11.08

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTEAAAARPRAVGTPAIWDARGVLLMSWAHFAHDLYPSFLGVLVPAIQAKLGVSLFWAS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMVPAQQLPSIVQPFLGYVADRTSRRWFVVLSPAVAALALSSIGIAPHFAVALVLLLVSG
HCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LASAAFHAPAVALVGEFGGQRLGRAMAIFMAGGELARTVAPILLTAVIAHFTLDGMPLVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LVGLAASATLFLRLRTEASDAIARRHAGQVQLWRLIRERQRPFIGLLGSVVANTAAVTPP
HHHHHHHHEEEEEEECCHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCH
SFFLVEYLLQRGRSEWYGGLALSAFYASGIIGGLVGGSLSDWVGRRGALLLSSLGTAPLL
HHHHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHCCHHHHH
ALYLAIENGSWLMLPLLVLIGVFATPPRSVTLALANDIAPEARGPLSGFTLATSFVAQSI
HHHHEECCCCCHHHHHHHHHHHHCCCCCCEEEEEHHCCCCCCCCCCCHHHHHHHHHHHHH
IALAFGAVAEAIGIERAFWLTVGASLAGVPFALLIPATMGRSPSSTG
HHHHHHHHHHHHHCCHHHHHHHCCHHHCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GTEAAAARPRAVGTPAIWDARGVLLMSWAHFAHDLYPSFLGVLVPAIQAKLGVSLFWAS
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMVPAQQLPSIVQPFLGYVADRTSRRWFVVLSPAVAALALSSIGIAPHFAVALVLLLVSG
HCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LASAAFHAPAVALVGEFGGQRLGRAMAIFMAGGELARTVAPILLTAVIAHFTLDGMPLVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LVGLAASATLFLRLRTEASDAIARRHAGQVQLWRLIRERQRPFIGLLGSVVANTAAVTPP
HHHHHHHHEEEEEEECCHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCH
SFFLVEYLLQRGRSEWYGGLALSAFYASGIIGGLVGGSLSDWVGRRGALLLSSLGTAPLL
HHHHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHCCHHHHH
ALYLAIENGSWLMLPLLVLIGVFATPPRSVTLALANDIAPEARGPLSGFTLATSFVAQSI
HHHHEECCCCCHHHHHHHHHHHHCCCCCCEEEEEHHCCCCCCCCCCCHHHHHHHHHHHHH
IALAFGAVAEAIGIERAFWLTVGASLAGVPFALLIPATMGRSPSSTG
HHHHHHHHHHHHHCCHHHHHHHCCHHHCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: fosmidomycin [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: fosmidomycin [Cytoplasm] + Proton [Periplasm] = fosmidomycin [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9141694; 9384377 [H]