Click here to switch to the map view.

The map label for this gene is tig

Identifier: 221633186

GI number: 221633186

Start: 1191032

End: 1192504

Strand: Reverse

Name: tig

Synonym: trd_1203

Alternate gene names: 221633186

Gene position: 1192504-1191032 (Counterclockwise)

Preceding gene: 221633199

Following gene: 221633185

Centisome position: 59.54

GC content: 62.66

Gene sequence:

>1473_bases
GTGAAGATCACTGTCGAACACCTGCCGCAAAGCACGGTGCGCTTGGATATCGCGGCGGATCCTGAAGAATTCGACGCTGC
ACTCGAACGAGCGTTCCGGCGCATCAGCCAGCAGGTTCAGGTTCCTGGCTTTCGGCCAGGGCGCGCACCTCGGGCGCTCG
TCGAGCGACGAGTGGGGCGGGAGCTGATCGTCGCGGAAGCGCAGCGTGAACTCATGGATCGCTTGTATCGTGAGGCGCTC
CAGCAACACCGGCTGACCCCAGTCGCCGAGCCGGAGGTGGAGATCTATCAGGACGAACCGCTCGCCTTCCGCGTCGAAGT
GCAGGTCTATCCGCAAGTCGATCTCGACGGCTATCGAGATATCCGCGTCGAGCCGCGCGAGGTCGAGGTGACCGAGGAAG
AAATCGACCAGGTCATCGAGGGGCTGCGGCGCAGTCGCGCGGTCTGGAAGACGCCGGACGAGCCGCGCCAGCCTCGCGAT
GGCGATCAGGTGATCGTCGATATCGAAGCGTACGAAGGCGAGCAACCGTTCCAGGAGCCACTCCGCCAGGCGACTTTCGT
CTTGGGCGAGAGCAACCTTTTTGCGGAGATCGATCAGGCTATCCGCTCCCTGCGTCCCGGAGAATCGGCTGAGTTCGATA
TTTCTTTCGCTGAAGAGGACGAGCGCGTCAGTCCGGAACTCCGTGGGAAGACGCTGCATTACCGCGTGACCGTACACGAG
GTGAAGGAAGCAGAGCTTCCGGAAGTGAATGACGAGTTCGCGCAGTCGCTAGGGGTGGCAACATTGAGCGAACTACGCGA
TCGCGTGCGGCGTGATCTCCTGCGCGAGAAGGCGCAGGCAGCGCGTGCGGAAGTGCTGGAACAGGCAGTCCAGCGTCTCC
TCGAGGTGGCGACGGTCGAGCTTCCGCCAGCGCTGATCGAGCGTCAGGTCGCGGCGGACGTCGAGCGGCTGCGTGAGCAA
CTGCGGCAGCGTGGAAGTAGTCTGGAAGAGTACCTTCGATTTCAGGGTAAAACCCTGGAAGAGTTCAAGGAAGAGTTGCG
CCCCCAAGCCGAAGCGCGCCTCCGCCGGTATTTGGTGCTGGAAGCCTTTGCCGAGGCTGAGGGGATTGCGGTCAGTGAGG
AGGAGTTGGTCGCTGAGATCGAGCGACTGGCTCTGGCCAGTGGAAGTCCCGAGCAGTTCCGCGCATTCTACTCTGTTCCT
TCGGTGCGGAGCTATCTCGCTGACGAATTGCACGAGCGCAAGGTGAGCGAGCGGCTTTTGGAGCTCGTCACGGAAGGACG
AGGTGCTGTTATCGGCGAGGCGGCTCGAGTGCTGGCCGGAGCCGAACCGGCCGAGGGGACCGAGCCAGCTGCGGAAGAAG
CAGTGACGGCTCCGGAGGTTGTGGATGGCGAAACGACGCCAGCTTCCGAGTCAGCGGAATCGCTGGCGGTAACGGAGACT
GGATCCCGAGCGGATGATGACCAGGCATCCTGA

Upstream 100 bases:

>100_bases
TGTCTGCGTCGAGCAGCTCTCTGCCGATCCGGGCGAGAATTGGGTGCAGCGAGATGAGCCGAGGTTCGATCATGGTTTGT
CTTGACAGGGAGGGGAGTTC

Downstream 100 bases:

>100_bases
GGGTACGAGGAGGCTATGCGATGGAGCGAGACATCCGACCTGAACTCGTCATCCCGATGGTGATCGAAACGACAAGTCGT
GGTGAGCGTGCGTACGATAT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MKITVEHLPQSTVRLDIAADPEEFDAALERAFRRISQQVQVPGFRPGRAPRALVERRVGRELIVAEAQRELMDRLYREAL
QQHRLTPVAEPEVEIYQDEPLAFRVEVQVYPQVDLDGYRDIRVEPREVEVTEEEIDQVIEGLRRSRAVWKTPDEPRQPRD
GDQVIVDIEAYEGEQPFQEPLRQATFVLGESNLFAEIDQAIRSLRPGESAEFDISFAEEDERVSPELRGKTLHYRVTVHE
VKEAELPEVNDEFAQSLGVATLSELRDRVRRDLLREKAQAARAEVLEQAVQRLLEVATVELPPALIERQVAADVERLREQ
LRQRGSSLEEYLRFQGKTLEEFKEELRPQAEARLRRYLVLEAFAEAEGIAVSEEELVAEIERLALASGSPEQFRAFYSVP
SVRSYLADELHERKVSERLLELVTEGRGAVIGEAARVLAGAEPAEGTEPAAEEAVTAPEVVDGETTPASESAESLAVTET
GSRADDDQAS

Sequences:

>Translated_490_residues
MKITVEHLPQSTVRLDIAADPEEFDAALERAFRRISQQVQVPGFRPGRAPRALVERRVGRELIVAEAQRELMDRLYREAL
QQHRLTPVAEPEVEIYQDEPLAFRVEVQVYPQVDLDGYRDIRVEPREVEVTEEEIDQVIEGLRRSRAVWKTPDEPRQPRD
GDQVIVDIEAYEGEQPFQEPLRQATFVLGESNLFAEIDQAIRSLRPGESAEFDISFAEEDERVSPELRGKTLHYRVTVHE
VKEAELPEVNDEFAQSLGVATLSELRDRVRRDLLREKAQAARAEVLEQAVQRLLEVATVELPPALIERQVAADVERLREQ
LRQRGSSLEEYLRFQGKTLEEFKEELRPQAEARLRRYLVLEAFAEAEGIAVSEEELVAEIERLALASGSPEQFRAFYSVP
SVRSYLADELHERKVSERLLELVTEGRGAVIGEAARVLAGAEPAEGTEPAAEEAVTAPEVVDGETTPASESAESLAVTET
GSRADDDQAS
>Mature_490_residues
MKITVEHLPQSTVRLDIAADPEEFDAALERAFRRISQQVQVPGFRPGRAPRALVERRVGRELIVAEAQRELMDRLYREAL
QQHRLTPVAEPEVEIYQDEPLAFRVEVQVYPQVDLDGYRDIRVEPREVEVTEEEIDQVIEGLRRSRAVWKTPDEPRQPRD
GDQVIVDIEAYEGEQPFQEPLRQATFVLGESNLFAEIDQAIRSLRPGESAEFDISFAEEDERVSPELRGKTLHYRVTVHE
VKEAELPEVNDEFAQSLGVATLSELRDRVRRDLLREKAQAARAEVLEQAVQRLLEVATVELPPALIERQVAADVERLREQ
LRQRGSSLEEYLRFQGKTLEEFKEELRPQAEARLRRYLVLEAFAEAEGIAVSEEELVAEIERLALASGSPEQFRAFYSVP
SVRSYLADELHERKVSERLLELVTEGRGAVIGEAARVLAGAEPAEGTEPAAEEAVTAPEVVDGETTPASESAESLAVTET
GSRADDDQAS

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=405, Percent_Identity=28.1481481481481, Blast_Score=145, Evalue=7e-36,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_THERP (B9L1E4)

Other databases:

- EMBL:   CP001275
- RefSeq:   YP_002522411.1
- GeneID:   7356318
- GenomeReviews:   CP001275_GR
- KEGG:   tro:trd_1203
- OMA:   RFIPGFE
- ProtClustDB:   CLSK2807892
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 55327; Mature: 55327

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITVEHLPQSTVRLDIAADPEEFDAALERAFRRISQQVQVPGFRPGRAPRALVERRVGR
CEEEHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCH
ELIVAEAQRELMDRLYREALQQHRLTPVAEPEVEIYQDEPLAFRVEVQVYPQVDLDGYRD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEEEEEEECCCCCCCCCC
IRVEPREVEVTEEEIDQVIEGLRRSRAVWKTPDEPRQPRDGDQVIVDIEAYEGEQPFQEP
CCCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHH
LRQATFVLGESNLFAEIDQAIRSLRPGESAEFDISFAEEDERVSPELRGKTLHYRVTVHE
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHCCCEEEEEEEHHH
VKEAELPEVNDEFAQSLGVATLSELRDRVRRDLLREKAQAARAEVLEQAVQRLLEVATVE
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LPPALIERQVAADVERLREQLRQRGSSLEEYLRFQGKTLEEFKEELRPQAEARLRRYLVL
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHH
EAFAEAEGIAVSEEELVAEIERLALASGSPEQFRAFYSVPSVRSYLADELHERKVSERLL
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ELVTEGRGAVIGEAARVLAGAEPAEGTEPAAEEAVTAPEVVDGETTPASESAESLAVTET
HHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEECC
GSRADDDQAS
CCCCCCCCCC
>Mature Secondary Structure
MKITVEHLPQSTVRLDIAADPEEFDAALERAFRRISQQVQVPGFRPGRAPRALVERRVGR
CEEEHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCH
ELIVAEAQRELMDRLYREALQQHRLTPVAEPEVEIYQDEPLAFRVEVQVYPQVDLDGYRD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEEEEEEECCCCCCCCCC
IRVEPREVEVTEEEIDQVIEGLRRSRAVWKTPDEPRQPRDGDQVIVDIEAYEGEQPFQEP
CCCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHH
LRQATFVLGESNLFAEIDQAIRSLRPGESAEFDISFAEEDERVSPELRGKTLHYRVTVHE
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHCCCEEEEEEEHHH
VKEAELPEVNDEFAQSLGVATLSELRDRVRRDLLREKAQAARAEVLEQAVQRLLEVATVE
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LPPALIERQVAADVERLREQLRQRGSSLEEYLRFQGKTLEEFKEELRPQAEARLRRYLVL
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHH
EAFAEAEGIAVSEEELVAEIERLALASGSPEQFRAFYSVPSVRSYLADELHERKVSERLL
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ELVTEGRGAVIGEAARVLAGAEPAEGTEPAAEEAVTAPEVVDGETTPASESAESLAVTET
HHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEECC
GSRADDDQAS
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA