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The map label for this gene is ispB [C]

Identifier: 221633181

GI number: 221633181

Start: 1186486

End: 1187520

Strand: Reverse

Name: ispB [C]

Synonym: trd_1197

Alternate gene names: 221633181

Gene position: 1187520-1186486 (Counterclockwise)

Preceding gene: 221633184

Following gene: 221633178

Centisome position: 59.29

GC content: 62.51

Gene sequence:

>1035_bases
GTGAACGCAGCGATCAGCGACGTGGAACGGACAATGCAAGAGTTGTTGGCCCGCTACGGCGCGCGATTTCCGGCGTTACC
GGGGGCGCTTCCCGTGTACGAGGTACTTGCCTATCATCTCGGCTGGCGCGACGAGCGGATGCAGCCAGCGAGCGGAGTCA
GCGGCAAGCGGTTTCGGCCTGCGCTCTGTACGGCTATCGCTTCTGCTGTCTGCGGTGATGCTGCTCGTGCTGTTGGCATC
GCGGCAGCGATCGAGCTGCTCCACAATTTCACCCTGATCCACGACGATATCCAGGATCGCAGTCGATTACGACGGCATCG
CCCCACGATTTGGGCCCTGTGGGGCGATGCTCAAGCGATCAATGCTGGCGATGCACTTTTCGTGTTGAGCCAGATCGCCG
CGCTGGAAGCGGCACAAGCACTCGAGCCCAGTCGTGGTGCGGAGCTCGTGCGCCGCTTCAACGAGACGGCACTTCTGATC
GTCGAAGGACAGGTTCTCGATCTCAGTTTCGAACAACGCCCCGAAGTGTCGGTCAACGAGTACCTCACCATGATCGAACG
GAAGACGGCTGCGCTGATCGCCTATTCCGCGTGGGCTGGGGCTCTGGTGGCTGGCGCGCCAGCGGAGGTCGCGGCACGGT
TGGAGCGCTTCGGGCGTGCGCTGGGACTCGGTTTTCAGATCCGCGACGACTACCTCGGTGTCTGGGGCGATCCGGCGGTT
ACAGGAAAGCCCCGAGGCGATGACCTCCGACAACGCAAGAAGAGCTTGCCGATCGTTCTCGTCCTCGAACGCGCTGCAGC
CAGTGATCGGGCCTGGTTACTGGATCTCTGGCGTCGACGAACTGAGCTGAGCGCGGAGGAAGTGGCTGCGGTCATCGAGC
TCCTGGAACGCTACGCGGTGCGTACTGCTCTGCAGGAAGCAGTCGAACGGTTCCATGGCGAAGCGTTGGCAGAGCTCGAA
GCGCTGTCGCTCACACCCGAAGGGGTTGCTTCTTTACAAGGGCTCGTCGCCGGATTGCCCGTGCGCGAAGCCTAG

Upstream 100 bases:

>100_bases
CTGTGCGACGATCACTACAAGTATGGGCGTTCTCTGCTCCGAATGTCCAGTCGTCGACTGTCGAGTGGATCGATGAAAGG
TTCGATCGAAAGGGATGGCC

Downstream 100 bases:

>100_bases
TCCTCTTGGGGCAGCAGTGACGGTAGCCGGTCTCCCGTCTCGCGCTCGAGCAGCCGGAGCGTCGCCTCGATCAAGCCGTA
ACAGCCGGTCACCATCATGT

Product: isoprenyl synthetase

Products: NA

Alternate protein names: GGPP synthase; GGPPSase; (2E,6E)-farnesyl diphosphate synthase; Dimethylallyltranstransferase; Farnesyl diphosphate synthase; Farnesyltranstransferase; Geranylgeranyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MNAAISDVERTMQELLARYGARFPALPGALPVYEVLAYHLGWRDERMQPASGVSGKRFRPALCTAIASAVCGDAARAVGI
AAAIELLHNFTLIHDDIQDRSRLRRHRPTIWALWGDAQAINAGDALFVLSQIAALEAAQALEPSRGAELVRRFNETALLI
VEGQVLDLSFEQRPEVSVNEYLTMIERKTAALIAYSAWAGALVAGAPAEVAARLERFGRALGLGFQIRDDYLGVWGDPAV
TGKPRGDDLRQRKKSLPIVLVLERAAASDRAWLLDLWRRRTELSAEEVAAVIELLERYAVRTALQEAVERFHGEALAELE
ALSLTPEGVASLQGLVAGLPVREA

Sequences:

>Translated_344_residues
MNAAISDVERTMQELLARYGARFPALPGALPVYEVLAYHLGWRDERMQPASGVSGKRFRPALCTAIASAVCGDAARAVGI
AAAIELLHNFTLIHDDIQDRSRLRRHRPTIWALWGDAQAINAGDALFVLSQIAALEAAQALEPSRGAELVRRFNETALLI
VEGQVLDLSFEQRPEVSVNEYLTMIERKTAALIAYSAWAGALVAGAPAEVAARLERFGRALGLGFQIRDDYLGVWGDPAV
TGKPRGDDLRQRKKSLPIVLVLERAAASDRAWLLDLWRRRTELSAEEVAAVIELLERYAVRTALQEAVERFHGEALAELE
ALSLTPEGVASLQGLVAGLPVREA
>Mature_344_residues
MNAAISDVERTMQELLARYGARFPALPGALPVYEVLAYHLGWRDERMQPASGVSGKRFRPALCTAIASAVCGDAARAVGI
AAAIELLHNFTLIHDDIQDRSRLRRHRPTIWALWGDAQAINAGDALFVLSQIAALEAAQALEPSRGAELVRRFNETALLI
VEGQVLDLSFEQRPEVSVNEYLTMIERKTAALIAYSAWAGALVAGAPAEVAARLERFGRALGLGFQIRDDYLGVWGDPAV
TGKPRGDDLRQRKKSLPIVLVLERAAASDRAWLLDLWRRRTELSAEEVAAVIELLERYAVRTALQEAVERFHGEALAELE
ALSLTPEGVASLQGLVAGLPVREA

Specific function: Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate which is a precursor of the ether-linked lipids [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=295, Percent_Identity=27.4576271186441, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI4758430, Length=282, Percent_Identity=25.531914893617, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI83700220, Length=282, Percent_Identity=25.531914893617, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI209571582, Length=259, Percent_Identity=30.8880308880309, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI4503685, Length=259, Percent_Identity=30.8880308880309, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI209571584, Length=259, Percent_Identity=30.8880308880309, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1789578, Length=205, Percent_Identity=34.1463414634146, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1786623, Length=266, Percent_Identity=32.7067669172932, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17508563, Length=331, Percent_Identity=25.0755287009063, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17505681, Length=200, Percent_Identity=27, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6325188, Length=211, Percent_Identity=28.9099526066351, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24651612, Length=218, Percent_Identity=30.7339449541284, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI281365769, Length=285, Percent_Identity=28.0701754385965, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24660002, Length=285, Percent_Identity=28.0701754385965, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17137582, Length=210, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.1; =2.5.1.29; =2.5.1.10 [H]

Molecular weight: Translated: 37572; Mature: 37572

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAAISDVERTMQELLARYGARFPALPGALPVYEVLAYHLGWRDERMQPASGVSGKRFRP
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
ALCTAIASAVCGDAARAVGIAAAIELLHNFTLIHDDIQDRSRLRRHRPTIWALWGDAQAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCC
NAGDALFVLSQIAALEAAQALEPSRGAELVRRFNETALLIVEGQVLDLSFEQRPEVSVNE
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECEEEECCCCCCCCCCHHH
YLTMIERKTAALIAYSAWAGALVAGAPAEVAARLERFGRALGLGFQIRDDYLGVWGDPAV
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCC
TGKPRGDDLRQRKKSLPIVLVLERAAASDRAWLLDLWRRRTELSAEEVAAVIELLERYAV
CCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RTALQEAVERFHGEALAELEALSLTPEGVASLQGLVAGLPVREA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNAAISDVERTMQELLARYGARFPALPGALPVYEVLAYHLGWRDERMQPASGVSGKRFRP
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
ALCTAIASAVCGDAARAVGIAAAIELLHNFTLIHDDIQDRSRLRRHRPTIWALWGDAQAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCC
NAGDALFVLSQIAALEAAQALEPSRGAELVRRFNETALLIVEGQVLDLSFEQRPEVSVNE
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECEEEECCCCCCCCCCHHH
YLTMIERKTAALIAYSAWAGALVAGAPAEVAARLERFGRALGLGFQIRDDYLGVWGDPAV
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCC
TGKPRGDDLRQRKKSLPIVLVLERAAASDRAWLLDLWRRRTELSAEEVAAVIELLERYAV
CCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RTALQEAVERFHGEALAELEALSLTPEGVASLQGLVAGLPVREA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]