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The map label for this gene is garL [H]

Identifier: 221633113

GI number: 221633113

Start: 1114757

End: 1115542

Strand: Reverse

Name: garL [H]

Synonym: trd_1129

Alternate gene names: 221633113

Gene position: 1115542-1114757 (Counterclockwise)

Preceding gene: 221633114

Following gene: 221633112

Centisome position: 55.69

GC content: 62.21

Gene sequence:

>786_bases
ATGCGGGAGAACCACGCCAAGCGGAAGATGTTGGCTGGCCAGCCGGCCTTCGGCTATACGCTGCAGCTCGGCGCACCGCT
CGTCGCAGAGGCACTGGCTAATTGCGGGATCGACTTCATCTTGATCGATACGCAGCATGGCACCTTCGGCCCGGATACGG
TTATCGCCACGCTGATGGCGCTTTCCTGCGGGCGCGCGGTGCCTATGGCTCGGGTCGCACGCAACGATTACACACTGATC
GGGCGCTTACTGGACGACGGGGTGCTCGGCATCATCGTTCCGCTGGTCCACACCCGAGAGGATGCGCGAGCGGTTGCCGA
CGCTTGCCGCTTTCCGCCGCGCGGTATGCGTTCGTGGGGATGGGGACGTGCTCGGGTCTATGGAGCTGATTATCCAGAAT
GGATCGACGAGGAGCTCTTCGTTGGTGTGCAAATCGAAAGTGCCCAGGCAGTGGAGAATGCCGAGGCGATTCTCTCGGTT
CCCGGTATCGACGGTTGCTGGATCGGGCCCGGCGATCTTGCTCTGTCTCTCGGAATCGACCCGCGTCGGGCGGCTGGTGA
CGAGCGGCAGGAGCGCGCGATCGAACGCGTGCTGGAAGCTTGTCGGAATACGGGCAAAATCGCGGGTTACGCTGCCTATA
GTGTCGAAGACGCCTTGTACCGAGCGCAGCAGGGTTTCCGATTCGTCACGGCCGGTAGCGATATCGGCTTCCTCGTGCAA
GGAGCGCTGCGTGGCGTCGAACAGCTCGGTCTGACTGCACAGGTCGGACGAGGATATGGGGAATAA

Upstream 100 bases:

>100_bases
AATGTTTGTCGAGCGCGGAGAAACCGCTCGTGGAACTTCACGGGATTCGACTGCTCGGGTATGGTGAGACGGGAAAGCAA
TGATGACGGGGGGCATAAGT

Downstream 100 bases:

>100_bases
GGGACTCGCGCAGAACCGGCAAGCACTGCTCAAGGCGTTCCAGGCGAGTCTGAAGGCGCGGCCGGGTGTAGAGTACATCA
ATTTGGTGATCTTCCGGCCG

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MRENHAKRKMLAGQPAFGYTLQLGAPLVAEALANCGIDFILIDTQHGTFGPDTVIATLMALSCGRAVPMARVARNDYTLI
GRLLDDGVLGIIVPLVHTREDARAVADACRFPPRGMRSWGWGRARVYGADYPEWIDEELFVGVQIESAQAVENAEAILSV
PGIDGCWIGPGDLALSLGIDPRRAAGDERQERAIERVLEACRNTGKIAGYAAYSVEDALYRAQQGFRFVTAGSDIGFLVQ
GALRGVEQLGLTAQVGRGYGE

Sequences:

>Translated_261_residues
MRENHAKRKMLAGQPAFGYTLQLGAPLVAEALANCGIDFILIDTQHGTFGPDTVIATLMALSCGRAVPMARVARNDYTLI
GRLLDDGVLGIIVPLVHTREDARAVADACRFPPRGMRSWGWGRARVYGADYPEWIDEELFVGVQIESAQAVENAEAILSV
PGIDGCWIGPGDLALSLGIDPRRAAGDERQERAIERVLEACRNTGKIAGYAAYSVEDALYRAQQGFRFVTAGSDIGFLVQ
GALRGVEQLGLTAQVGRGYGE
>Mature_261_residues
MRENHAKRKMLAGQPAFGYTLQLGAPLVAEALANCGIDFILIDTQHGTFGPDTVIATLMALSCGRAVPMARVARNDYTLI
GRLLDDGVLGIIVPLVHTREDARAVADACRFPPRGMRSWGWGRARVYGADYPEWIDEELFVGVQIESAQAVENAEAILSV
PGIDGCWIGPGDLALSLGIDPRRAAGDERQERAIERVLEACRNTGKIAGYAAYSVEDALYRAQQGFRFVTAGSDIGFLVQ
GALRGVEQLGLTAQVGRGYGE

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=238, Percent_Identity=34.0336134453782, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI1788578, Length=242, Percent_Identity=33.4710743801653, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 28105; Mature: 28105

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRENHAKRKMLAGQPAFGYTLQLGAPLVAEALANCGIDFILIDTQHGTFGPDTVIATLMA
CCCCHHHHHHHCCCCCCCEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHH
LSCGRAVPMARVARNDYTLIGRLLDDGVLGIIVPLVHTREDARAVADACRFPPRGMRSWG
HHCCCCCHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
WGRARVYGADYPEWIDEELFVGVQIESAQAVENAEAILSVPGIDGCWIGPGDLALSLGID
CCCEEEECCCCHHHCCCCEEEEEEECCHHHHHCCHHEEECCCCCCCEECCCCEEEEECCC
PRRAAGDERQERAIERVLEACRNTGKIAGYAAYSVEDALYRAQQGFRFVTAGSDIGFLVQ
CHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCEEEECCCCHHHHHH
GALRGVEQLGLTAQVGRGYGE
HHHHHHHHHCCCHHHCCCCCC
>Mature Secondary Structure
MRENHAKRKMLAGQPAFGYTLQLGAPLVAEALANCGIDFILIDTQHGTFGPDTVIATLMA
CCCCHHHHHHHCCCCCCCEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHH
LSCGRAVPMARVARNDYTLIGRLLDDGVLGIIVPLVHTREDARAVADACRFPPRGMRSWG
HHCCCCCHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
WGRARVYGADYPEWIDEELFVGVQIESAQAVENAEAILSVPGIDGCWIGPGDLALSLGID
CCCEEEECCCCHHHCCCCEEEEEEECCHHHHHCCHHEEECCCCCCCEECCCCEEEEECCC
PRRAAGDERQERAIERVLEACRNTGKIAGYAAYSVEDALYRAQQGFRFVTAGSDIGFLVQ
CHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCEEEECCCCHHHHHH
GALRGVEQLGLTAQVGRGYGE
HHHHHHHHHCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA