| Definition | Thermomicrobium roseum DSM 5159 chromosome, complete genome. |
|---|---|
| Accession | NC_011959 |
| Length | 2,003,006 |
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The map label for this gene is yitJ [H]
Identifier: 221633034
GI number: 221633034
Start: 1017849
End: 1019723
Strand: Reverse
Name: yitJ [H]
Synonym: trd_1049
Alternate gene names: 221633034
Gene position: 1019723-1017849 (Counterclockwise)
Preceding gene: 221633037
Following gene: 221633033
Centisome position: 50.91
GC content: 61.81
Gene sequence:
>1875_bases ATGAAGCATCCGTTTCGTGACCGGATCGAACAAGGTGTTCTCCTCGCTGACGGCGCCATGGGTACGCAGCTGTACGAGCG AGGAATCCGGTTGGATCGTTGCTTCGAGTCGGCAAACCTGACTCATCCGCAACTCGTCGTGGAGATCCACCGGGCCTACA TTCTGGCGGGAGCCGAACTCATCGAGACCAATACGTTCGGAGCCAACCGCTTCAAGCTCGAGGCCTACGGCATGGCGGAG CGTGTGCGTGAGATCAATCGCCGTGCTGTCCGTCATGCGCGAGAGGCACGGGAGATTTGTGGTGTGCCGGTTTTCATCGC GGGCGCCATAGGACCGAGCGGACGCCTGCTCGCACCGGTGGGGACAGTTGCACCGGAGAGCTTACGACCAGTTTTCCGCG AGCAGATCGAAGCGTTGCTCGAGGGTGGGGTTGATCTCCTCATTTTCGAGACCATGTCCCAACTCGCTGAGATCCGCGAA GCGATCATGGCTGCACGTGAGGTCTGTGATCTCCCGATCGTCGCCCAGATGACGTTTGCGGCTGACGGTCGCACCATCGC TGGGCACGGTCCGGAAGAAGTGGTGGCCGCCTTAGCGGAACTGGGCGTCGATGTGATCGGCGTCAACTGCAGTGTCGGCC CACGGCCGACGCTCGAGGTGCTGCGGGCCATGATCGCTGCGAATCGCTGGGGTACCGCGCTTTCGGCGATGCCGAATGCG GGTTGGCCGACGGTCGTCGGTCATCGCGTCATTTATGGTGCATCGCCGGAGTACTTTGCCGATTTCGCGAGGGAGGCAGC ATCCTTGGGTGTTCGACTCATCGGCGGTTGCTGTGGGACGACACCCGAACACATCGCGGCAATGCGGCGTGCTCTGGACG ACATCAAAGGACAAGTACGTCCAGCGGTCGAGGTCAGGGCGCGCGTTTCAACACCGGTAATGGACGCAGTCGCACCGGAG GAGCCCACGCGCCTCAGGCAGAAACTGGGGCGGGAGTTCGTGGTGAGCGTGGAAATCGATCCTCCCAAGGGGCTCAATCC CTCCAAAGCGATCGAAGGAGCCCGTCTGCTGCGCCAGGTGGGCGTCGAGTTCATCAACGTGGCCGACAGTCCAATGGCAC GAGTGCGCATGAGCGCGCTGGCGCTCTGCGTGATTCTCCAGCAGGAGACAGGCGTCGAGACGATCCTGCATCTGACGACT CGCGATCGGAATCTGATGGGGTTACAGGCCGATCTCCTGGGTGCGCACGCGCTCGGTGTGCGCAACATTCTCGCTCTGAC CGGCGATCCACCGACGTTGGGCGATTATCCCCATGCGACGCCCGTCTACGACCTCGACTCGATCGGTCTGGTGCGTTTGC TCCAGAATTTCCGGCAGGGATTCGATGCCGGTGGTGCCTCCATCGGTCGACCAGCGATGTTCACGATCGGAGTCGCGTGT GATCCGACGCGTCCGGATCTTCAGCAGGAGATCGAGCGTTTGCATGCGAAGCTTGCGGCTGGGGCTGATTTCGTCATGAC ACAACCGGTCTTCGACTTCTCGACGTGGCGCAACTTCGTCGCACAGTACGAGATTCGCTACGGACCGATCGAGGTGCCGG TGCTGTTGGGAGTGCTACCGTTGCAGAGCTTTCGGCATGCCTCCTTCCTGCACAACGAAGTTCCCGGCATAACGTTGACC GAGGAGGCACTGGAGCGCATGCGGCGAGCTGGTCCGAACGGGCGAGCCGAAGGAGTGGCCATGGCGCGCGAGTTGATCGC TGAGGCCTGCGAGTTCGTGCAGGGTATCTATCTCATGCCCTCTTTCGGCCGCTACGAGGTGGTGGCTGAGGTGTTGAGCG CCCTCCCCGAAAGATTGCGCGTCAGCACCCGCTGA
Upstream 100 bases:
>100_bases CTCGTCACCGCACAGTGGTGGCCGGCACGGCGATGATGGTCCGCGTACACTCGATAGCTAGGTGGCTGGGTCAGCACTCG ATTGGTGGAGATTGCGAGAC
Downstream 100 bases:
>100_bases TTCTGGTGGAACGCTCTGCTATACTGCCAACTGGTCGTCTTTCTGCGACCGCGGGAGCGGTGCCGCAGGCTGGGAGAGCG CGGGATAAGGAACACAGCCA
Product: bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein
Products: NA
Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]
Number of amino acids: Translated: 624; Mature: 624
Protein sequence:
>624_residues MKHPFRDRIEQGVLLADGAMGTQLYERGIRLDRCFESANLTHPQLVVEIHRAYILAGAELIETNTFGANRFKLEAYGMAE RVREINRRAVRHAREAREICGVPVFIAGAIGPSGRLLAPVGTVAPESLRPVFREQIEALLEGGVDLLIFETMSQLAEIRE AIMAAREVCDLPIVAQMTFAADGRTIAGHGPEEVVAALAELGVDVIGVNCSVGPRPTLEVLRAMIAANRWGTALSAMPNA GWPTVVGHRVIYGASPEYFADFAREAASLGVRLIGGCCGTTPEHIAAMRRALDDIKGQVRPAVEVRARVSTPVMDAVAPE EPTRLRQKLGREFVVSVEIDPPKGLNPSKAIEGARLLRQVGVEFINVADSPMARVRMSALALCVILQQETGVETILHLTT RDRNLMGLQADLLGAHALGVRNILALTGDPPTLGDYPHATPVYDLDSIGLVRLLQNFRQGFDAGGASIGRPAMFTIGVAC DPTRPDLQQEIERLHAKLAAGADFVMTQPVFDFSTWRNFVAQYEIRYGPIEVPVLLGVLPLQSFRHASFLHNEVPGITLT EEALERMRRAGPNGRAEGVAMARELIAEACEFVQGIYLMPSFGRYEVVAEVLSALPERLRVSTR
Sequences:
>Translated_624_residues MKHPFRDRIEQGVLLADGAMGTQLYERGIRLDRCFESANLTHPQLVVEIHRAYILAGAELIETNTFGANRFKLEAYGMAE RVREINRRAVRHAREAREICGVPVFIAGAIGPSGRLLAPVGTVAPESLRPVFREQIEALLEGGVDLLIFETMSQLAEIRE AIMAAREVCDLPIVAQMTFAADGRTIAGHGPEEVVAALAELGVDVIGVNCSVGPRPTLEVLRAMIAANRWGTALSAMPNA GWPTVVGHRVIYGASPEYFADFAREAASLGVRLIGGCCGTTPEHIAAMRRALDDIKGQVRPAVEVRARVSTPVMDAVAPE EPTRLRQKLGREFVVSVEIDPPKGLNPSKAIEGARLLRQVGVEFINVADSPMARVRMSALALCVILQQETGVETILHLTT RDRNLMGLQADLLGAHALGVRNILALTGDPPTLGDYPHATPVYDLDSIGLVRLLQNFRQGFDAGGASIGRPAMFTIGVAC DPTRPDLQQEIERLHAKLAAGADFVMTQPVFDFSTWRNFVAQYEIRYGPIEVPVLLGVLPLQSFRHASFLHNEVPGITLT EEALERMRRAGPNGRAEGVAMARELIAEACEFVQGIYLMPSFGRYEVVAEVLSALPERLRVSTR >Mature_624_residues MKHPFRDRIEQGVLLADGAMGTQLYERGIRLDRCFESANLTHPQLVVEIHRAYILAGAELIETNTFGANRFKLEAYGMAE RVREINRRAVRHAREAREICGVPVFIAGAIGPSGRLLAPVGTVAPESLRPVFREQIEALLEGGVDLLIFETMSQLAEIRE AIMAAREVCDLPIVAQMTFAADGRTIAGHGPEEVVAALAELGVDVIGVNCSVGPRPTLEVLRAMIAANRWGTALSAMPNA GWPTVVGHRVIYGASPEYFADFAREAASLGVRLIGGCCGTTPEHIAAMRRALDDIKGQVRPAVEVRARVSTPVMDAVAPE EPTRLRQKLGREFVVSVEIDPPKGLNPSKAIEGARLLRQVGVEFINVADSPMARVRMSALALCVILQQETGVETILHLTT RDRNLMGLQADLLGAHALGVRNILALTGDPPTLGDYPHATPVYDLDSIGLVRLLQNFRQGFDAGGASIGRPAMFTIGVAC DPTRPDLQQEIERLHAKLAAGADFVMTQPVFDFSTWRNFVAQYEIRYGPIEVPVLLGVLPLQSFRHASFLHNEVPGITLT EEALERMRRAGPNGRAEGVAMARELIAEACEFVQGIYLMPSFGRYEVVAEVLSALPERLRVSTR
Specific function: Terminal step in the de novo biosynthesis of methionine. [C]
COG id: COG0685
COG function: function code E; 5,10-methylenetetrahydrofolate reductase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Hcy-binding domain [H]
Homologues:
Organism=Homo sapiens, GI169790923, Length=348, Percent_Identity=30.4597701149425, Blast_Score=141, Evalue=2e-33, Organism=Homo sapiens, GI157266337, Length=366, Percent_Identity=27.3224043715847, Blast_Score=106, Evalue=8e-23, Organism=Homo sapiens, GI13162290, Length=319, Percent_Identity=27.5862068965517, Blast_Score=100, Evalue=4e-21, Organism=Escherichia coli, GI1790450, Length=347, Percent_Identity=31.1239193083574, Blast_Score=137, Evalue=2e-33, Organism=Escherichia coli, GI1786456, Length=312, Percent_Identity=31.7307692307692, Blast_Score=97, Evalue=3e-21, Organism=Caenorhabditis elegans, GI17535405, Length=329, Percent_Identity=31.6109422492401, Blast_Score=144, Evalue=1e-34, Organism=Caenorhabditis elegans, GI25147436, Length=284, Percent_Identity=27.112676056338, Blast_Score=67, Evalue=3e-11, Organism=Caenorhabditis elegans, GI25147441, Length=284, Percent_Identity=26.4084507042254, Blast_Score=67, Evalue=3e-11, Organism=Saccharomyces cerevisiae, GI6322966, Length=314, Percent_Identity=23.8853503184713, Blast_Score=80, Evalue=1e-15, Organism=Saccharomyces cerevisiae, GI6324982, Length=311, Percent_Identity=23.7942122186495, Blast_Score=79, Evalue=1e-15,
Paralogues:
None
Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003171 - InterPro: IPR003726 [H]
Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]
EC number: =2.1.1.10; =1.5.1.20 [H]
Molecular weight: Translated: 67967; Mature: 67967
Theoretical pI: Translated: 6.22; Mature: 6.22
Prosite motif: PS50970 HCY
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.4 %Cys (Translated Protein) 2.9 %Met (Translated Protein) 4.3 %Cys+Met (Translated Protein) 1.4 %Cys (Mature Protein) 2.9 %Met (Mature Protein) 4.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKHPFRDRIEQGVLLADGAMGTQLYERGIRLDRCFESANLTHPQLVVEIHRAYILAGAEL CCCCHHHHHHCCEEEECCCHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHH IETNTFGANRFKLEAYGMAERVREINRRAVRHAREAREICGVPVFIAGAIGPSGRLLAPV HCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEECCC GTVAPESLRPVFREQIEALLEGGVDLLIFETMSQLAEIREAIMAAREVCDLPIVAQMTFA CCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEC ADGRTIAGHGPEEVVAALAELGVDVIGVNCSVGPRPTLEVLRAMIAANRWGTALSAMPNA CCCCEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHCCCCC GWPTVVGHRVIYGASPEYFADFAREAASLGVRLIGGCCGTTPEHIAAMRRALDDIKGQVR CCCHHHCCEEEECCCHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC PAVEVRARVSTPVMDAVAPEEPTRLRQKLGREFVVSVEIDPPKGLNPSKAIEGARLLRQV CHHHHHHHHCCCHHHHCCCCCHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHH GVEFINVADSPMARVRMSALALCVILQQETGVETILHLTTRDRNLMGLQADLLGAHALGV CHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHEECCCCCCCCCCHHHHHHHHHHHH RNILALTGDPPTLGDYPHATPVYDLDSIGLVRLLQNFRQGFDAGGASIGRPAMFTIGVAC HHEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEC DPTRPDLQQEIERLHAKLAAGADFVMTQPVFDFSTWRNFVAQYEIRYGPIEVPVLLGVLP CCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHEECCCCHHHHHHHHHH LQSFRHASFLHNEVPGITLTEEALERMRRAGPNGRAEGVAMARELIAEACEFVQGIYLMP HHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCEEECC SFGRYEVVAEVLSALPERLRVSTR CCCHHHHHHHHHHHHHHHHCCCCC >Mature Secondary Structure MKHPFRDRIEQGVLLADGAMGTQLYERGIRLDRCFESANLTHPQLVVEIHRAYILAGAEL CCCCHHHHHHCCEEEECCCHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHH IETNTFGANRFKLEAYGMAERVREINRRAVRHAREAREICGVPVFIAGAIGPSGRLLAPV HCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEECCC GTVAPESLRPVFREQIEALLEGGVDLLIFETMSQLAEIREAIMAAREVCDLPIVAQMTFA CCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEC ADGRTIAGHGPEEVVAALAELGVDVIGVNCSVGPRPTLEVLRAMIAANRWGTALSAMPNA CCCCEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHCCCCC GWPTVVGHRVIYGASPEYFADFAREAASLGVRLIGGCCGTTPEHIAAMRRALDDIKGQVR CCCHHHCCEEEECCCHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC PAVEVRARVSTPVMDAVAPEEPTRLRQKLGREFVVSVEIDPPKGLNPSKAIEGARLLRQV CHHHHHHHHCCCHHHHCCCCCHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHH GVEFINVADSPMARVRMSALALCVILQQETGVETILHLTTRDRNLMGLQADLLGAHALGV CHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHEECCCCCCCCCCHHHHHHHHHHHH RNILALTGDPPTLGDYPHATPVYDLDSIGLVRLLQNFRQGFDAGGASIGRPAMFTIGVAC HHEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEC DPTRPDLQQEIERLHAKLAAGADFVMTQPVFDFSTWRNFVAQYEIRYGPIEVPVLLGVLP CCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHEECCCCHHHHHHHHHH LQSFRHASFLHNEVPGITLTEEALERMRRAGPNGRAEGVAMARELIAEACEFVQGIYLMP HHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCEEECC SFGRYEVVAEVLSALPERLRVSTR CCCHHHHHHHHHHHHHHHHCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9353932; 9384377 [H]