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The map label for this gene is 221633022

Identifier: 221633022

GI number: 221633022

Start: 1006056

End: 1007174

Strand: Reverse

Name: 221633022

Synonym: trd_1037

Alternate gene names: NA

Gene position: 1007174-1006056 (Counterclockwise)

Preceding gene: 221633023

Following gene: 221633021

Centisome position: 50.28

GC content: 62.65

Gene sequence:

>1119_bases
ATGACGGTGACGATCCGCAACCTCAGGATTGCCGGGCCAACGCCGTTACCGCCAGCGGTCGTCGAGGCGTTGCAGCGTCC
GATGGTGCCGCATCGCGGTGGATGGTTCCGGGGTTTGGTGCGGTCTCTGCTGGAGCGACTGCGCGCACTGCATCGGACGG
ACGGAGACGTTTTCGTCATGCCCGGCACCGGATCGGCGGGTTGGGAGATTGCATTGGTCAATTTGCTCGCCCCGGGTGAT
CGGGTCCTGCTGCTCGTGAACGGGGACTTCGGTGAGCGATGGCGGCGGGTGGCGGAGCGGTATGGCCTGCAGTTGATCGT
GCGCGAGGTTCCTTACGGGAGGGCAATCAAGCCGAGTGATGTTGCAGGGTGGCTCGCGGAGACCGAGCCAGTGCGAGCTG
TTTTGCTGGTCTATAACGAGACTTCGACGGGGGTCACGAATCCGCTTCGAGAGATCGCTGCTGTCGTGCGGGATGCTGGT
GCGCTCTTGGTGGTGGACGGCGTGAGTGCAGTGGCTGGACTGCCGCTGGAGATGGATGAGTGGGGAGTGGATCTCATCTT
CAGTGGCTCCCAGAAGGCGTGGATGTGCCCGCCAGGGCTGGTCATCGTCGCCGTCGGATCGCGAGCCTGGGAGATGATCG
ACCGGGCCAGCTATCCACGCGCTTTTTGGGATCTCCGGGAGTACCGGTCTGCCGCACGGACAGGAGACCTGCCGTCGACT
GCACCGATCAGTCTGATCTATGGCTTGGACGCAGCGGTCCAGCTGATCGAAGCGGAGGGCCTAGAGAACGTCTGGACGAG
ACACCGAGATCTCGCGGTGAGATTTCGCCACGTCACGAGCGAGTTGGGCTTTACCTGCTTCGCAGAAGGTGGCTACGAGA
GCGCTACAGTGACGGCACTCGTCCCGCCTGCGGGCATCGGTGCCGAGGAGTTGGTTTGCAGCCTGGAAGAGCGTTACGGG
ATCGCGGTTAATGGTGGTCAAGGGCGCTTGAAGGGACAGATCATTCGGGTAGGGCATATGGGGTGGGTGCGCTGGAGCGA
TCTCGAGGAGGTGTGTGCGGCTTTGGCCAGTGAAGTGGAGCGAGCAGTTGCTGGTCGAAGCGAAGCAGTTGGACACTGA

Upstream 100 bases:

>100_bases
GCTTCCCGCACATGGTTGCGACCTTTTGGTTGACGCAGGCGATAGTGCTGGTCGCTGGCACACGATCGACGGGAACACAG
CACACAGAAGGGGGAACAGC

Downstream 100 bases:

>100_bases
CCGAACTGGCTGTAGCTCAGGGAACCTGACTGGCGTGTCAAGCGAGGTGCTTGTCGTGATCGCGAGTTCCCGGTATACTA
GCGGCGGTGTCTCGCTAGCC

Product: soluble hydrogenase 42 kDa subunit

Products: NA

Alternate protein names: Tritium exchange subunit [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MTVTIRNLRIAGPTPLPPAVVEALQRPMVPHRGGWFRGLVRSLLERLRALHRTDGDVFVMPGTGSAGWEIALVNLLAPGD
RVLLLVNGDFGERWRRVAERYGLQLIVREVPYGRAIKPSDVAGWLAETEPVRAVLLVYNETSTGVTNPLREIAAVVRDAG
ALLVVDGVSAVAGLPLEMDEWGVDLIFSGSQKAWMCPPGLVIVAVGSRAWEMIDRASYPRAFWDLREYRSAARTGDLPST
APISLIYGLDAAVQLIEAEGLENVWTRHRDLAVRFRHVTSELGFTCFAEGGYESATVTALVPPAGIGAEELVCSLEERYG
IAVNGGQGRLKGQIIRVGHMGWVRWSDLEEVCAALASEVERAVAGRSEAVGH

Sequences:

>Translated_372_residues
MTVTIRNLRIAGPTPLPPAVVEALQRPMVPHRGGWFRGLVRSLLERLRALHRTDGDVFVMPGTGSAGWEIALVNLLAPGD
RVLLLVNGDFGERWRRVAERYGLQLIVREVPYGRAIKPSDVAGWLAETEPVRAVLLVYNETSTGVTNPLREIAAVVRDAG
ALLVVDGVSAVAGLPLEMDEWGVDLIFSGSQKAWMCPPGLVIVAVGSRAWEMIDRASYPRAFWDLREYRSAARTGDLPST
APISLIYGLDAAVQLIEAEGLENVWTRHRDLAVRFRHVTSELGFTCFAEGGYESATVTALVPPAGIGAEELVCSLEERYG
IAVNGGQGRLKGQIIRVGHMGWVRWSDLEEVCAALASEVERAVAGRSEAVGH
>Mature_371_residues
TVTIRNLRIAGPTPLPPAVVEALQRPMVPHRGGWFRGLVRSLLERLRALHRTDGDVFVMPGTGSAGWEIALVNLLAPGDR
VLLLVNGDFGERWRRVAERYGLQLIVREVPYGRAIKPSDVAGWLAETEPVRAVLLVYNETSTGVTNPLREIAAVVRDAGA
LLVVDGVSAVAGLPLEMDEWGVDLIFSGSQKAWMCPPGLVIVAVGSRAWEMIDRASYPRAFWDLREYRSAARTGDLPSTA
PISLIYGLDAAVQLIEAEGLENVWTRHRDLAVRFRHVTSELGFTCFAEGGYESATVTALVPPAGIGAEELVCSLEERYGI
AVNGGQGRLKGQIIRVGHMGWVRWSDLEEVCAALASEVERAVAGRSEAVGH

Specific function: Soluble hydrogenase catalyzes both production and consumption of hydrogen from suitable artificial electron donors or acceptors. This subunit catalyzes the tritium-exchange activity [H]

COG id: COG0075

COG function: function code E; Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557289, Length=380, Percent_Identity=29.7368421052632, Blast_Score=155, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17536281, Length=370, Percent_Identity=30, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321079, Length=364, Percent_Identity=28.2967032967033, Blast_Score=129, Evalue=7e-31,
Organism=Drosophila melanogaster, GI17530823, Length=350, Percent_Identity=27.7142857142857, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: NA

Molecular weight: Translated: 40431; Mature: 40300

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTIRNLRIAGPTPLPPAVVEALQRPMVPHRGGWFRGLVRSLLERLRALHRTDGDVFVM
CEEEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
PGTGSAGWEIALVNLLAPGDRVLLLVNGDFGERWRRVAERYGLQLIVREVPYGRAIKPSD
CCCCCCCCEEEEEEEECCCCEEEEEEECCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCH
VAGWLAETEPVRAVLLVYNETSTGVTNPLREIAAVVRDAGALLVVDGVSAVAGLPLEMDE
HHHHHCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHCCCCCCCC
WGVDLIFSGSQKAWMCPPGLVIVAVGSRAWEMIDRASYPRAFWDLREYRSAARTGDLPST
CCEEEEEECCCCEEECCCCEEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
APISLIYGLDAAVQLIEAEGLENVWTRHRDLAVRFRHVTSELGFTCFAEGGYESATVTAL
CCCCEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEE
VPPAGIGAEELVCSLEERYGIAVNGGQGRLKGQIIRVGHMGWVRWSDLEEVCAALASEVE
ECCCCCCHHHHHHHHHHHCCEEEECCCCCEEEEEEEEECCCCEEECCHHHHHHHHHHHHH
RAVAGRSEAVGH
HHHCCCHHCCCC
>Mature Secondary Structure 
TVTIRNLRIAGPTPLPPAVVEALQRPMVPHRGGWFRGLVRSLLERLRALHRTDGDVFVM
EEEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
PGTGSAGWEIALVNLLAPGDRVLLLVNGDFGERWRRVAERYGLQLIVREVPYGRAIKPSD
CCCCCCCCEEEEEEEECCCCEEEEEEECCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCH
VAGWLAETEPVRAVLLVYNETSTGVTNPLREIAAVVRDAGALLVVDGVSAVAGLPLEMDE
HHHHHCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHCCCCCCCC
WGVDLIFSGSQKAWMCPPGLVIVAVGSRAWEMIDRASYPRAFWDLREYRSAARTGDLPST
CCEEEEEECCCCEEECCCCEEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
APISLIYGLDAAVQLIEAEGLENVWTRHRDLAVRFRHVTSELGFTCFAEGGYESATVTAL
CCCCEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEE
VPPAGIGAEELVCSLEERYGIAVNGGQGRLKGQIIRVGHMGWVRWSDLEEVCAALASEVE
ECCCCCCHHHHHHHHHHHCCEEEECCCCCEEEEEEEEECCCCEEECCHHHHHHHHHHHHH
RAVAGRSEAVGH
HHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2513553 [H]