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The map label for this gene is tuf [H]

Identifier: 221632980

GI number: 221632980

Start: 946164

End: 947366

Strand: Reverse

Name: tuf [H]

Synonym: trd_0986

Alternate gene names: 221632980

Gene position: 947366-946164 (Counterclockwise)

Preceding gene: 221632981

Following gene: 221632979

Centisome position: 47.3

GC content: 65.09

Gene sequence:

>1203_bases
ATGAGCAAGCCGCGATTTGAGCGGACGAAGCCGCACGTGAATGTGGGGACGATTGGGCATGTGGATCATGGGAAGACGAC
GTTGACGGCGGCGATCACGAAGGTGTTGAGCTTCAAGGGGTGGGCGAACTTCAAGCCGTACGAGCAGATCGACAAGGCGC
CGGAGGAGCGGGCGCGGGGGATCACGATCGCGATCAGCCATGTGGAGTATGAGACGGAGAAGCGGCACTATGCGCATGTG
GATTGTCCGGGGCATGCGGACTACATCAAGAACATGATCACGGGTGCGGCGCAGATGGATGGGGCGATCTTGGTGGTGAG
TGCGCCGGATGGGCCGATGCCGCAGACGCGGGAGCATATTTTGCTGGCGCGGCAGGTGGAGGTGCCGGCGATCGTGGTGT
TTTTGAACAAGGTTGACATGCTGGATGATCCGGAGTTGTTGGAGCTGGTGGAGCTGGAGGTGCGGGAGCTGTTGAGCCAG
TATGGGTATCCGGGGGAGAGCGTGCCGGTGGTGCGGGGGTCGGCGCTGCGGGCGTTGGAGTCGGCGTCGACCGATCCGGA
GGCGCCGGAGTATGCGCCGATCTGGGAGCTGTTGCGGGTGGTGGACGAGTACATCCCGACGCCGGTGCGGGCGGTGGACA
AGCCGTTTTTGATGCCGATCGAGGACGTGTTTGGGATCAAGGGGCGCGGGACGGTGGTGACGGGCCGGGTCGAGCGTGGG
CGGCTGCGGCCTGGGGAGACGGTGGAGATTGTGGGGCTGGGGCCGACGCGGCAGACGGTGGTGACGTCGATCGAGATGTT
CCAGAAGGTATTGGACGAGGCGGTGGCGGGGGACAACATCGGGTGCTTGTTGCGGGGGATCGAGCGGGACGAGGTGGAGC
GGGGGCAGGTGCTGGCGGCGCCGGGGTCGATCACGCCGCATCGGGAGTTTGAGGCGGAGGTGTACGTGTTGTCGAAGGAG
GAGGGGGGGCGGCACACGCCGTTCTTTGCGGGGTATCGGCCGCAGTTTTACATTCGGACGACGGATGTGACGGGGGAAGT
GGTGGGGTTGCCGGAGGGGGTGGAGATGGTGATGCCGGGGGACAACGTGCGGCTGCGGGTGGAGTTGGACAAGCCGGTGG
CGCTGGAGGAAGGGTCGCGCTTTGCCATCCGGGAAGGTGGCCGCACGGTCGGCGCCGGCGTCGTCACCAAGATCATCAAG
TGA

Upstream 100 bases:

>100_bases
GAGAGCATCGCGCAAGAATTGATGGCCAAAGCTGGGAAGTCGAGTTGAGCGTGAGACGGTCGGACGTGAGTCGAAACGAG
TTGCTGAAAGGGGTAGCACG

Downstream 100 bases:

>100_bases
GGGGTGGTCGGCGATGTCGCGGAGACCGACGCAGCGTATTCGAATCCGACTGAAATCGCACGATCACCGGATTCTCGACC
AGTCGGCGCGGCAGATCGTA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSKPRFERTKPHVNVGTIGHVDHGKTTLTAAITKVLSFKGWANFKPYEQIDKAPEERARGITIAISHVEYETEKRHYAHV
DCPGHADYIKNMITGAAQMDGAILVVSAPDGPMPQTREHILLARQVEVPAIVVFLNKVDMLDDPELLELVELEVRELLSQ
YGYPGESVPVVRGSALRALESASTDPEAPEYAPIWELLRVVDEYIPTPVRAVDKPFLMPIEDVFGIKGRGTVVTGRVERG
RLRPGETVEIVGLGPTRQTVVTSIEMFQKVLDEAVAGDNIGCLLRGIERDEVERGQVLAAPGSITPHREFEAEVYVLSKE
EGGRHTPFFAGYRPQFYIRTTDVTGEVVGLPEGVEMVMPGDNVRLRVELDKPVALEEGSRFAIREGGRTVGAGVVTKIIK

Sequences:

>Translated_400_residues
MSKPRFERTKPHVNVGTIGHVDHGKTTLTAAITKVLSFKGWANFKPYEQIDKAPEERARGITIAISHVEYETEKRHYAHV
DCPGHADYIKNMITGAAQMDGAILVVSAPDGPMPQTREHILLARQVEVPAIVVFLNKVDMLDDPELLELVELEVRELLSQ
YGYPGESVPVVRGSALRALESASTDPEAPEYAPIWELLRVVDEYIPTPVRAVDKPFLMPIEDVFGIKGRGTVVTGRVERG
RLRPGETVEIVGLGPTRQTVVTSIEMFQKVLDEAVAGDNIGCLLRGIERDEVERGQVLAAPGSITPHREFEAEVYVLSKE
EGGRHTPFFAGYRPQFYIRTTDVTGEVVGLPEGVEMVMPGDNVRLRVELDKPVALEEGSRFAIREGGRTVGAGVVTKIIK
>Mature_399_residues
SKPRFERTKPHVNVGTIGHVDHGKTTLTAAITKVLSFKGWANFKPYEQIDKAPEERARGITIAISHVEYETEKRHYAHVD
CPGHADYIKNMITGAAQMDGAILVVSAPDGPMPQTREHILLARQVEVPAIVVFLNKVDMLDDPELLELVELEVRELLSQY
GYPGESVPVVRGSALRALESASTDPEAPEYAPIWELLRVVDEYIPTPVRAVDKPFLMPIEDVFGIKGRGTVVTGRVERGR
LRPGETVEIVGLGPTRQTVVTSIEMFQKVLDEAVAGDNIGCLLRGIERDEVERGQVLAAPGSITPHREFEAEVYVLSKEE
GGRHTPFFAGYRPQFYIRTTDVTGEVVGLPEGVEMVMPGDNVRLRVELDKPVALEEGSRFAIREGGRTVGAGVVTKIIK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=398, Percent_Identity=57.035175879397, Blast_Score=466, Evalue=1e-131,
Organism=Homo sapiens, GI4503475, Length=451, Percent_Identity=32.1507760532151, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI4503471, Length=449, Percent_Identity=32.293986636971, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI223555963, Length=435, Percent_Identity=29.8850574712644, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI5729864, Length=435, Percent_Identity=29.8850574712644, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI194018522, Length=441, Percent_Identity=28.1179138321995, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI194097354, Length=441, Percent_Identity=28.1179138321995, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI194018520, Length=441, Percent_Identity=28.1179138321995, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI46094014, Length=441, Percent_Identity=27.6643990929705, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI54607086, Length=327, Percent_Identity=29.9694189602446, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI82546879, Length=431, Percent_Identity=25.9860788863109, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI4503507, Length=276, Percent_Identity=28.2608695652174, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI45593140, Length=432, Percent_Identity=25.6944444444444, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI53729339, Length=264, Percent_Identity=30.3030303030303, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI53729337, Length=264, Percent_Identity=30.3030303030303, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1790412, Length=399, Percent_Identity=70.6766917293233, Blast_Score=548, Evalue=1e-157,
Organism=Escherichia coli, GI1789737, Length=399, Percent_Identity=70.6766917293233, Blast_Score=547, Evalue=1e-157,
Organism=Escherichia coli, GI2367247, Length=384, Percent_Identity=27.8645833333333, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI48994988, Length=297, Percent_Identity=30.976430976431, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789108, Length=370, Percent_Identity=28.3783783783784, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788922, Length=256, Percent_Identity=26.171875, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=405, Percent_Identity=51.358024691358, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25141371, Length=391, Percent_Identity=43.9897698209719, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17552884, Length=451, Percent_Identity=31.7073170731707, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17569207, Length=451, Percent_Identity=31.7073170731707, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI32566303, Length=440, Percent_Identity=30, Blast_Score=148, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI32566629, Length=454, Percent_Identity=27.9735682819383, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=453, Percent_Identity=27.5938189845475, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=453, Percent_Identity=27.5938189845475, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17506081, Length=265, Percent_Identity=30.188679245283, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17509919, Length=449, Percent_Identity=26.5033407572383, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=39.4557823129252, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=236, Percent_Identity=25.8474576271186, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=400, Percent_Identity=66.25, Blast_Score=526, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6325337, Length=449, Percent_Identity=32.5167037861915, Blast_Score=180, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6319594, Length=449, Percent_Identity=32.5167037861915, Blast_Score=180, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6320377, Length=445, Percent_Identity=26.0674157303371, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322937, Length=452, Percent_Identity=22.3451327433628, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6320863, Length=161, Percent_Identity=32.2981366459627, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=244, Percent_Identity=28.2786885245902, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281363316, Length=397, Percent_Identity=58.1863979848867, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI17864358, Length=397, Percent_Identity=58.1863979848867, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI19921738, Length=390, Percent_Identity=50.2564102564103, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45553807, Length=451, Percent_Identity=31.7073170731707, Blast_Score=171, Evalue=8e-43,
Organism=Drosophila melanogaster, GI45553816, Length=451, Percent_Identity=31.7073170731707, Blast_Score=171, Evalue=8e-43,
Organism=Drosophila melanogaster, GI24651721, Length=451, Percent_Identity=31.7073170731707, Blast_Score=171, Evalue=8e-43,
Organism=Drosophila melanogaster, GI17864154, Length=451, Percent_Identity=31.7073170731707, Blast_Score=171, Evalue=8e-43,
Organism=Drosophila melanogaster, GI24652838, Length=450, Percent_Identity=31.3333333333333, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI17137572, Length=450, Percent_Identity=31.3333333333333, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI19922690, Length=308, Percent_Identity=32.1428571428571, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI17137380, Length=445, Percent_Identity=28.9887640449438, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI45550900, Length=440, Percent_Identity=29.5454545454545, Blast_Score=138, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24647048, Length=423, Percent_Identity=25.0591016548463, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24647046, Length=424, Percent_Identity=25, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922512, Length=424, Percent_Identity=23.5849056603774, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24654852, Length=424, Percent_Identity=23.5849056603774, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28572034, Length=193, Percent_Identity=36.2694300518135, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 44101; Mature: 43970

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPRFERTKPHVNVGTIGHVDHGKTTLTAAITKVLSFKGWANFKPYEQIDKAPEERARG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHCE
ITIAISHVEYETEKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVSAPDGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVEVPAIVVFLNKVDMLDDPELLELVELEVRELLSQYGYPGESVPVVRGSALRALE
EEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHH
SASTDPEAPEYAPIWELLRVVDEYIPTPVRAVDKPFLMPIEDVFGIKGRGTVVTGRVERG
HCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCC
RLRPGETVEIVGLGPTRQTVVTSIEMFQKVLDEAVAGDNIGCLLRGIERDEVERGQVLAA
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCHHHHCCCEEEEC
PGSITPHREFEAEVYVLSKEEGGRHTPFFAGYRPQFYIRTTDVTGEVVGLPEGVEMVMPG
CCCCCCCCCCCEEEEEEEECCCCCCCCEECCCCCEEEEEEECCCCEEEECCCCCEEEECC
DNVRLRVELDKPVALEEGSRFAIREGGRTVGAGVVTKIIK
CCEEEEEEECCCEEECCCCEEEEECCCCEEHHHHHHHHCC
>Mature Secondary Structure 
SKPRFERTKPHVNVGTIGHVDHGKTTLTAAITKVLSFKGWANFKPYEQIDKAPEERARG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHCE
ITIAISHVEYETEKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVSAPDGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVEVPAIVVFLNKVDMLDDPELLELVELEVRELLSQYGYPGESVPVVRGSALRALE
EEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHH
SASTDPEAPEYAPIWELLRVVDEYIPTPVRAVDKPFLMPIEDVFGIKGRGTVVTGRVERG
HCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCC
RLRPGETVEIVGLGPTRQTVVTSIEMFQKVLDEAVAGDNIGCLLRGIERDEVERGQVLAA
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCHHHHCCCEEEEC
PGSITPHREFEAEVYVLSKEEGGRHTPFFAGYRPQFYIRTTDVTGEVVGLPEGVEMVMPG
CCCCCCCCCCCEEEEEEEECCCCCCCCEECCCCCEEEEEEECCCCEEEECCCCCEEEECC
DNVRLRVELDKPVALEEGSRFAIREGGRTVGAGVVTKIIK
CCEEEEEEECCCEEECCCCEEEEECCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA