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The map label for this gene is truA [H]

Identifier: 221632949

GI number: 221632949

Start: 929714

End: 930553

Strand: Reverse

Name: truA [H]

Synonym: trd_0955

Alternate gene names: 221632949

Gene position: 930553-929714 (Counterclockwise)

Preceding gene: 221632950

Following gene: 221632948

Centisome position: 46.46

GC content: 64.52

Gene sequence:

>840_bases
ATGGTGCGCCAACGCTATCGGCTCGACTTCGGGTACGAGGGTACTGCGTTCAGTGGTTCCCAGCGCCAGCCGGGGCTGCG
CACCGTACAGGGAGTCTTGGAGGAGGCCTTGGCGCGGCTGGCTGGTGAGCCGGTTCGCGTTGCTTTGGCTGGTCGCACGG
ATGCGGGTGTGCACGCGGTTGGCCAAGTGGCGAGCTTCGATCTGTGCTGGCGCCATGACGAGGCGACACTGGCGCGAGCG
CTCCAAGCATGGTTGCCGGAGGATGTGGTGGTCTACCGGGTGGCTCGCATGCCGGCCGGTTTCCACGCGCGACGCGGGGC
TGTCGACCGCGAGTATCGTTACCGTATCTGGCAAGGAGAACAACCGCCGCTTTTCCTGCGGCGCTTCGTCTGGCGATTTG
CCGGACTACTCGACCTTCGGGCGATGCAGGAAGCGGCTCAGTTTTTTCTGGGAAGCCATGACTTTCGCTCGTTTGCCGGG
CACGGCCTCGGAGGACCCAAAGCGACAACTCCCCGCGTCCGGACAATCACGATGGCAGAATGGCATCTGCTCAGCCCGGT
GGTGGAGCCAGTCGGTACGGGCGCGCAGATCGTCGAATTTCGAGTCCGTGCCAATGGTTTCTTACCCCAAATGGTGCGTA
CGATGGTCTCAGCGTTGGTGCGGGTGGGGACCGGTGCGGCGGAACCGAGCTGGATCTCCGAGCTGCTCGAGCGCTGCGAT
CGACGAGCAGCACCACCGCCCGCGCCGCCGGGTGGCCTCGTCCTGTGGAGAGTTCGCTATCCTGAAGACATTGCATTGGC
CACGCCCGCTTCGCGGGCAGACGAAGAGAGTCGTGGGTGA

Upstream 100 bases:

>100_bases
GGTGGCTATACACGCATCTACCGGATCGGTCAACGACGCGGTGATGGCGCCCCCTTGGCGCTGATCGAGTTCGTCGCCTG
ATGCATGCGATGACCGCTGT

Downstream 100 bases:

>100_bases
TGCCTCCGAGAGCGAGGGATGAACGATGACGGTGAAAGCGAAAAAGCGACGTTACCTGCAGCGAACCTATATGGCCAAAC
CAGGAGAAGTCGAGCGGAAG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MVRQRYRLDFGYEGTAFSGSQRQPGLRTVQGVLEEALARLAGEPVRVALAGRTDAGVHAVGQVASFDLCWRHDEATLARA
LQAWLPEDVVVYRVARMPAGFHARRGAVDREYRYRIWQGEQPPLFLRRFVWRFAGLLDLRAMQEAAQFFLGSHDFRSFAG
HGLGGPKATTPRVRTITMAEWHLLSPVVEPVGTGAQIVEFRVRANGFLPQMVRTMVSALVRVGTGAAEPSWISELLERCD
RRAAPPPAPPGGLVLWRVRYPEDIALATPASRADEESRG

Sequences:

>Translated_279_residues
MVRQRYRLDFGYEGTAFSGSQRQPGLRTVQGVLEEALARLAGEPVRVALAGRTDAGVHAVGQVASFDLCWRHDEATLARA
LQAWLPEDVVVYRVARMPAGFHARRGAVDREYRYRIWQGEQPPLFLRRFVWRFAGLLDLRAMQEAAQFFLGSHDFRSFAG
HGLGGPKATTPRVRTITMAEWHLLSPVVEPVGTGAQIVEFRVRANGFLPQMVRTMVSALVRVGTGAAEPSWISELLERCD
RRAAPPPAPPGGLVLWRVRYPEDIALATPASRADEESRG
>Mature_279_residues
MVRQRYRLDFGYEGTAFSGSQRQPGLRTVQGVLEEALARLAGEPVRVALAGRTDAGVHAVGQVASFDLCWRHDEATLARA
LQAWLPEDVVVYRVARMPAGFHARRGAVDREYRYRIWQGEQPPLFLRRFVWRFAGLLDLRAMQEAAQFFLGSHDFRSFAG
HGLGGPKATTPRVRTITMAEWHLLSPVVEPVGTGAQIVEFRVRANGFLPQMVRTMVSALVRVGTGAAEPSWISELLERCD
RRAAPPPAPPGGLVLWRVRYPEDIALATPASRADEESRG

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=282, Percent_Identity=37.9432624113475, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI199559785, Length=280, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1788657, Length=266, Percent_Identity=37.593984962406, Blast_Score=149, Evalue=3e-37,
Organism=Drosophila melanogaster, GI116007752, Length=303, Percent_Identity=27.7227722772277, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 31011; Mature: 31011

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRQRYRLDFGYEGTAFSGSQRQPGLRTVQGVLEEALARLAGEPVRVALAGRTDAGVHAV
CCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHH
GQVASFDLCWRHDEATLARALQAWLPEDVVVYRVARMPAGFHARRGAVDREYRYRIWQGE
HHHHHHHHHEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHCCCCCCHHHEEEECCC
QPPLFLRRFVWRFAGLLDLRAMQEAAQFFLGSHDFRSFAGHGLGGPKATTPRVRTITMAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCEEEEEHHH
WHLLSPVVEPVGTGAQIVEFRVRANGFLPQMVRTMVSALVRVGTGAAEPSWISELLERCD
HHHHHHHHHHCCCCHHEEEEEHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RRAAPPPAPPGGLVLWRVRYPEDIALATPASRADEESRG
HCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MVRQRYRLDFGYEGTAFSGSQRQPGLRTVQGVLEEALARLAGEPVRVALAGRTDAGVHAV
CCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHH
GQVASFDLCWRHDEATLARALQAWLPEDVVVYRVARMPAGFHARRGAVDREYRYRIWQGE
HHHHHHHHHEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHCCCCCCHHHEEEECCC
QPPLFLRRFVWRFAGLLDLRAMQEAAQFFLGSHDFRSFAGHGLGGPKATTPRVRTITMAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCEEEEEHHH
WHLLSPVVEPVGTGAQIVEFRVRANGFLPQMVRTMVSALVRVGTGAAEPSWISELLERCD
HHHHHHHHHHCCCCHHEEEEEHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RRAAPPPAPPGGLVLWRVRYPEDIALATPASRADEESRG
HCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA