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The map label for this gene is lhgO [H]

Identifier: 221632887

GI number: 221632887

Start: 869471

End: 870673

Strand: Reverse

Name: lhgO [H]

Synonym: trd_0887

Alternate gene names: 221632887

Gene position: 870673-869471 (Counterclockwise)

Preceding gene: 221632888

Following gene: 221632885

Centisome position: 43.47

GC content: 62.76

Gene sequence:

>1203_bases
ATGTTCGATGTCGTCGTGGTGGGTGCTGGACTGGTCGGACTCGCCACTGCCCGCGAACTTCTGCTTCGTTTTCCACGCGT
GAGGCTCGCGGTCGTGGAGAAAGAGGGGAAAGTCGCGGCGCACCAGTCGGGACACAACAGCGGCGTGATCCATTCGGGCC
TGTACTACCGACCGGGCTCCCTGAAGGCGCGCGCTTGTGTGGCCGGCGCAGCGAAGCTGATCCGCTACTGCGAGGAACGG
GGTATTCCGTATCGGTTGATCGGTAAATTGGTGGTGGCGACGAATCCCGACGAGATTCCACGCTTGCTCGAACTCTTCCA
ACGGGGACAAGCGAACGGGGTTCAGGGATTGCAGCTCCTCGGTCCGGAAGAGATACGAGAGCGTGAACCGGCCGTCACGG
GGGTACGCGCGATCTGGTCGCCACGGACGGGTATCGTCGACTTCGTTCGTGTGGCGGAGGCACTCGCCGACGATATTCGC
ACGGCGGGTGGGGAAATTCGCTTGGGTCACCACGTGATGGCTTTCCGGCAGGGCAACGGTACGATCGCGATCGAAACGAG
TGCTGGTTGCCTAGAGACACGCTTCGCTCTGGTGTGTGCTGGTCTGTTCAGCGACCGCTTGGCACGAGCGAGCGGTGGAG
GACCTGACCCCGCCATCGTGCCCTTCCGTGGGGACTACTATGTTCTCCGTCCGGAACGCAGTCACCTCGTGCGCACCAAC
GTCTATCCGGTCCCCGATCCGCGCTTTCCCTTCCTCGGTGTCCATTTCACCCCCCGCTTGGATGGTTCGGTGTGGCTCGG
GCCGAATGCCGTTCTGGCCTTCGCCCGCGAGGGATATCGTCGAACAGACGTCGACTGGCACGATCTCTGGGAAGCGGTTT
CCTATCCTGGTTTTCGTATCTTGGCTCGTCGATACTGGCGTGTCGGCCTCATGGAGCTCTGGCGCGATTTTAGCCGGCGA
GCCTTTCTCCGGGAGCTCCAGCGTTTCGTTCCGGAGCTCGAAGAAGATGACCTCCTTCCCGGCCCGTCCGGTGTGCGGGC
GCAAGCCGTGACCCGGGACGGCCAGCTCGTGGACGACTTCGTGTTGGAGCGGCATGGCCGCGTGGTCCATGTCCGCAATG
CTCCTTCACCGGCAGCGACCTCTTGTCTGGAAATCGCGCGGCTGCTGGTCGACGAACTGGCTGAGAGTCTGCCGACTCGC
TGA

Upstream 100 bases:

>100_bases
ACTTCGAGCGTGGTCTGCATCGTCCAGAGGTCTGCAGCGCCTGTGGGAACCGCACCTTCACGCTCGAGCGCGAGCCACCG
GGAATGTGAGGATGAGTGGA

Downstream 100 bases:

>100_bases
TGGTCCTAACGAGGTCGCTGGCTCATCGAAAGACCTGATCCCACTTGTGCGTGCGCTGCTGGGTGATGCCGGAAGCGCAA
CGAGAAGTACGCGACTGCCA

Product: putative FAD dependent oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MFDVVVVGAGLVGLATARELLLRFPRVRLAVVEKEGKVAAHQSGHNSGVIHSGLYYRPGSLKARACVAGAAKLIRYCEER
GIPYRLIGKLVVATNPDEIPRLLELFQRGQANGVQGLQLLGPEEIREREPAVTGVRAIWSPRTGIVDFVRVAEALADDIR
TAGGEIRLGHHVMAFRQGNGTIAIETSAGCLETRFALVCAGLFSDRLARASGGGPDPAIVPFRGDYYVLRPERSHLVRTN
VYPVPDPRFPFLGVHFTPRLDGSVWLGPNAVLAFAREGYRRTDVDWHDLWEAVSYPGFRILARRYWRVGLMELWRDFSRR
AFLRELQRFVPELEEDDLLPGPSGVRAQAVTRDGQLVDDFVLERHGRVVHVRNAPSPAATSCLEIARLLVDELAESLPTR

Sequences:

>Translated_400_residues
MFDVVVVGAGLVGLATARELLLRFPRVRLAVVEKEGKVAAHQSGHNSGVIHSGLYYRPGSLKARACVAGAAKLIRYCEER
GIPYRLIGKLVVATNPDEIPRLLELFQRGQANGVQGLQLLGPEEIREREPAVTGVRAIWSPRTGIVDFVRVAEALADDIR
TAGGEIRLGHHVMAFRQGNGTIAIETSAGCLETRFALVCAGLFSDRLARASGGGPDPAIVPFRGDYYVLRPERSHLVRTN
VYPVPDPRFPFLGVHFTPRLDGSVWLGPNAVLAFAREGYRRTDVDWHDLWEAVSYPGFRILARRYWRVGLMELWRDFSRR
AFLRELQRFVPELEEDDLLPGPSGVRAQAVTRDGQLVDDFVLERHGRVVHVRNAPSPAATSCLEIARLLVDELAESLPTR
>Mature_400_residues
MFDVVVVGAGLVGLATARELLLRFPRVRLAVVEKEGKVAAHQSGHNSGVIHSGLYYRPGSLKARACVAGAAKLIRYCEER
GIPYRLIGKLVVATNPDEIPRLLELFQRGQANGVQGLQLLGPEEIREREPAVTGVRAIWSPRTGIVDFVRVAEALADDIR
TAGGEIRLGHHVMAFRQGNGTIAIETSAGCLETRFALVCAGLFSDRLARASGGGPDPAIVPFRGDYYVLRPERSHLVRTN
VYPVPDPRFPFLGVHFTPRLDGSVWLGPNAVLAFAREGYRRTDVDWHDLWEAVSYPGFRILARRYWRVGLMELWRDFSRR
AFLRELQRFVPELEEDDLLPGPSGVRAQAVTRDGQLVDDFVLERHGRVVHVRNAPSPAATSCLEIARLLVDELAESLPTR

Specific function: Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate [H]

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L2HGDH family [H]

Homologues:

Organism=Homo sapiens, GI13376331, Length=411, Percent_Identity=48.9051094890511, Blast_Score=395, Evalue=1e-110,
Organism=Escherichia coli, GI87082148, Length=401, Percent_Identity=42.8927680798005, Blast_Score=328, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI17565764, Length=401, Percent_Identity=48.6284289276808, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24585081, Length=392, Percent_Identity=48.9795918367347, Blast_Score=380, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.3.15 [H]

Molecular weight: Translated: 44374; Mature: 44374

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDVVVVGAGLVGLATARELLLRFPRVRLAVVEKEGKVAAHQSGHNSGVIHSGLYYRPGS
CCEEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCEEECCCCCCCCCEECCCEECCCC
LKARACVAGAAKLIRYCEERGIPYRLIGKLVVATNPDEIPRLLELFQRGQANGVQGLQLL
CCHHHHHHHHHHHHHHHHHCCCCHHHCCEEEEECCHHHHHHHHHHHHCCCCCCCCCEEEC
GPEEIREREPAVTGVRAIWSPRTGIVDFVRVAEALADDIRTAGGEIRLGHHVMAFRQGNG
CHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEEEECCC
TIAIETSAGCLETRFALVCAGLFSDRLARASGGGPDPAIVPFRGDYYVLRPERSHLVRTN
EEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEECCCCCCEEECC
VYPVPDPRFPFLGVHFTPRLDGSVWLGPNAVLAFAREGYRRTDVDWHDLWEAVSYPGFRI
CCCCCCCCCCEEEEEECCCCCCCEEECCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHH
LARRYWRVGLMELWRDFSRRAFLRELQRFVPELEEDDLLPGPSGVRAQAVTRDGQLVDDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHH
VLERHGRVVHVRNAPSPAATSCLEIARLLVDELAESLPTR
HHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFDVVVVGAGLVGLATARELLLRFPRVRLAVVEKEGKVAAHQSGHNSGVIHSGLYYRPGS
CCEEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCEEECCCCCCCCCEECCCEECCCC
LKARACVAGAAKLIRYCEERGIPYRLIGKLVVATNPDEIPRLLELFQRGQANGVQGLQLL
CCHHHHHHHHHHHHHHHHHCCCCHHHCCEEEEECCHHHHHHHHHHHHCCCCCCCCCEEEC
GPEEIREREPAVTGVRAIWSPRTGIVDFVRVAEALADDIRTAGGEIRLGHHVMAFRQGNG
CHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEEEECCC
TIAIETSAGCLETRFALVCAGLFSDRLARASGGGPDPAIVPFRGDYYVLRPERSHLVRTN
EEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEECCCCCCEEECC
VYPVPDPRFPFLGVHFTPRLDGSVWLGPNAVLAFAREGYRRTDVDWHDLWEAVSYPGFRI
CCCCCCCCCCEEEEEECCCCCCCEEECCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHH
LARRYWRVGLMELWRDFSRRAFLRELQRFVPELEEDDLLPGPSGVRAQAVTRDGQLVDDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHH
VLERHGRVVHVRNAPSPAATSCLEIARLLVDELAESLPTR
HHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]