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The map label for this gene is tyrS [H]

Identifier: 221632775

GI number: 221632775

Start: 756039

End: 757256

Strand: Reverse

Name: tyrS [H]

Synonym: trd_0768

Alternate gene names: 221632775

Gene position: 757256-756039 (Counterclockwise)

Preceding gene: 221632776

Following gene: 221632772

Centisome position: 37.81

GC content: 60.1

Gene sequence:

>1218_bases
ATGATCCCGGTTCATGAGCGAGAAGCGTTCGTTGATCTGCTCCTCTCGCGCGGCACGGTCGATGTCGTCGTCCGCGAGCA
CTTGCGTGCCCGGCTGCTCGGCGAGCAGCCGCTCCGCGTCAAGCTCGGGGTCGATCCGACCCGACCAGACATCCACCTCG
GTCACTACGTCTGTTTTCGCAAGCTCCGGCAGTTCCAAGCATTGGGTCATCAGGTCGTCGTCATCATCGGTGACTGGACT
GCCCGAATCGGCGATCCCTCCGGGCGCTCGTCCCAGCGGCCGATGCTCACCGCAGAAGAAGTACGAGCCAACGCGGAAAC
TTATCTCGAGCAATTCTTCAAGGTTGTCGATGTGACGAAGGCCGAGATCGTTTGGCAAAGCACCTGGTTCGGTAATTTCA
CGCTGGAGGATGTCATTCGCCTCGCCAGTAAGTACACCGTTGCCAAGCTACTCACGCGCGAGGACTTCGCGAAGCGCTAC
GAGGAAGGATCGCCCATCTCCATCACCGAGCTTCTCTATCCACTCCTGCAAGCCTATGACTCGGTGGCGATCCAAGCCGA
CGTCGAAATCGGTGGCACCGACCAGCTCTTCAATCTCCTCGTCGGCCGCGATATCATGCGCGAGTACGGATTGGAACCCC
AGGACATCCTCACCGTACCGCTCCTCGTCGGTACGGACGGCCATCTGAAGATGAGCAAGAGTTATGGCAATTACATCGCG
GTCAATGATCCACCAGAGGAGATGTTCGGCAAGATCATGTCGATTCCCGATCATGTTTTAGGCGACTATTTTCGGCTCCT
GACCGACATCCCAGACGAAGAGATCGACCGCATGATCGTGGAGATGCGCGCGGGACGGCTCAATCCACGCGACGCCAAGG
AGCATCTCGCGTTCGTCATCGTCAGTGACCTGCACTCGCCCGAAGCAGCTGAGCAGGCGCGCGAGCACTTCCACCGAGTA
CACCGGCTCCGAGAAATCCCCGAGCAGATTCCCGAGGTTCCCGTGCCGGGACGCGCACGTGTTCTCGATTATGTCGTGCT
AGCGGGGTTCGCCCGCAGTAACAACGAGGCACGGCGACTCGTCCAGCAGGGCGGGGTCCGCTTGGACGGACGGCGACTCG
ACGATCCGGAGGAAATGCTGGAACTCCAGGAGCCAGTCGTGCTCCAAGTCGGTAAGCGACGATTTGCGCGCCTCCTGCCA
CGAGCGGAGAGCACGTGA

Upstream 100 bases:

>100_bases
GAACGGAACGGGCGAGCTGTACTTCAGGCAGCGATGGCCGATTGGCCACCACCGCTCGTACAAGCGCTCCACGCAGCCCT
GGAAGGAGTCGAGGTGATCG

Downstream 100 bases:

>100_bases
TTCAGTGCTCGAACACGACGATGCTTCGCGCGACGCTGCCCGCGAGCATCGCTTCGAATCCGTCGTTGATCTCCTCCAGG
CGAAGTCGCCGGGAAATCAG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MIPVHEREAFVDLLLSRGTVDVVVREHLRARLLGEQPLRVKLGVDPTRPDIHLGHYVCFRKLRQFQALGHQVVVIIGDWT
ARIGDPSGRSSQRPMLTAEEVRANAETYLEQFFKVVDVTKAEIVWQSTWFGNFTLEDVIRLASKYTVAKLLTREDFAKRY
EEGSPISITELLYPLLQAYDSVAIQADVEIGGTDQLFNLLVGRDIMREYGLEPQDILTVPLLVGTDGHLKMSKSYGNYIA
VNDPPEEMFGKIMSIPDHVLGDYFRLLTDIPDEEIDRMIVEMRAGRLNPRDAKEHLAFVIVSDLHSPEAAEQAREHFHRV
HRLREIPEQIPEVPVPGRARVLDYVVLAGFARSNNEARRLVQQGGVRLDGRRLDDPEEMLELQEPVVLQVGKRRFARLLP
RAEST

Sequences:

>Translated_405_residues
MIPVHEREAFVDLLLSRGTVDVVVREHLRARLLGEQPLRVKLGVDPTRPDIHLGHYVCFRKLRQFQALGHQVVVIIGDWT
ARIGDPSGRSSQRPMLTAEEVRANAETYLEQFFKVVDVTKAEIVWQSTWFGNFTLEDVIRLASKYTVAKLLTREDFAKRY
EEGSPISITELLYPLLQAYDSVAIQADVEIGGTDQLFNLLVGRDIMREYGLEPQDILTVPLLVGTDGHLKMSKSYGNYIA
VNDPPEEMFGKIMSIPDHVLGDYFRLLTDIPDEEIDRMIVEMRAGRLNPRDAKEHLAFVIVSDLHSPEAAEQAREHFHRV
HRLREIPEQIPEVPVPGRARVLDYVVLAGFARSNNEARRLVQQGGVRLDGRRLDDPEEMLELQEPVVLQVGKRRFARLLP
RAEST
>Mature_405_residues
MIPVHEREAFVDLLLSRGTVDVVVREHLRARLLGEQPLRVKLGVDPTRPDIHLGHYVCFRKLRQFQALGHQVVVIIGDWT
ARIGDPSGRSSQRPMLTAEEVRANAETYLEQFFKVVDVTKAEIVWQSTWFGNFTLEDVIRLASKYTVAKLLTREDFAKRY
EEGSPISITELLYPLLQAYDSVAIQADVEIGGTDQLFNLLVGRDIMREYGLEPQDILTVPLLVGTDGHLKMSKSYGNYIA
VNDPPEEMFGKIMSIPDHVLGDYFRLLTDIPDEEIDRMIVEMRAGRLNPRDAKEHLAFVIVSDLHSPEAAEQAREHFHRV
HRLREIPEQIPEVPVPGRARVLDYVVLAGFARSNNEARRLVQQGGVRLDGRRLDDPEEMLELQEPVVLQVGKRRFARLLP
RAEST

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=401, Percent_Identity=27.6807980049875, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1787925, Length=413, Percent_Identity=26.3922518159806, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25145802, Length=291, Percent_Identity=27.1477663230241, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6325160, Length=436, Percent_Identity=24.5412844036697, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI20130383, Length=400, Percent_Identity=23.5, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46164; Mature: 46164

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPVHEREAFVDLLLSRGTVDVVVREHLRARLLGEQPLRVKLGVDPTRPDIHLGHYVCFR
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHH
KLRQFQALGHQVVVIIGDWTARIGDPSGRSSQRPMLTAEEVRANAETYLEQFFKVVDVTK
HHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHH
AEIVWQSTWFGNFTLEDVIRLASKYTVAKLLTREDFAKRYEEGSPISITELLYPLLQAYD
HHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHC
SVAIQADVEIGGTDQLFNLLVGRDIMREYGLEPQDILTVPLLVGTDGHLKMSKSYGNYIA
CEEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCCEEEECCCCCEEE
VNDPPEEMFGKIMSIPDHVLGDYFRLLTDIPDEEIDRMIVEMRAGRLNPRDAKEHLAFVI
ECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHEEHE
VSDLHSPEAAEQAREHFHRVHRLREIPEQIPEVPVPGRARVLDYVVLAGFARSNNEARRL
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
VQQGGVRLDGRRLDDPEEMLELQEPVVLQVGKRRFARLLPRAEST
HHHCCCEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIPVHEREAFVDLLLSRGTVDVVVREHLRARLLGEQPLRVKLGVDPTRPDIHLGHYVCFR
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHH
KLRQFQALGHQVVVIIGDWTARIGDPSGRSSQRPMLTAEEVRANAETYLEQFFKVVDVTK
HHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHH
AEIVWQSTWFGNFTLEDVIRLASKYTVAKLLTREDFAKRYEEGSPISITELLYPLLQAYD
HHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHC
SVAIQADVEIGGTDQLFNLLVGRDIMREYGLEPQDILTVPLLVGTDGHLKMSKSYGNYIA
CEEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCCEEEECCCCCEEE
VNDPPEEMFGKIMSIPDHVLGDYFRLLTDIPDEEIDRMIVEMRAGRLNPRDAKEHLAFVI
ECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHEEHE
VSDLHSPEAAEQAREHFHRVHRLREIPEQIPEVPVPGRARVLDYVVLAGFARSNNEARRL
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
VQQGGVRLDGRRLDDPEEMLELQEPVVLQVGKRRFARLLPRAEST
HHHCCCEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]