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The map label for this gene is dnaB [H]

Identifier: 221632704

GI number: 221632704

Start: 682293

End: 683645

Strand: Reverse

Name: dnaB [H]

Synonym: trd_0692

Alternate gene names: 221632704

Gene position: 683645-682293 (Counterclockwise)

Preceding gene: 221632705

Following gene: 221632703

Centisome position: 34.13

GC content: 60.9

Gene sequence:

>1353_bases
ATGACGAGCACGACTGCCGAGGCCATCGAGCGGTTACCGCCGCACAATATCGAGGCCGAGCAGGCCGTGCTCGGTAGCCT
GCTCATCGATCGCGACGCCATCATTCGGGTTGCTTCCTTCCTCCGACCTGACGATTTCTTTCGCGGGAGCCATAGTATCA
TCTACCAGGCTCTGCTCGACCTTTACAACCGGCGGGTACCCGCCGACTTCGTGACGGTCGTCGACGAACTCGAACGGACC
GGACGCCTCGACGATGCCGGCGGCGTCGCCTACCTCACTGAGCTCCTCAGCGTGGTGCCGAGCGCCGTCCACGTCGAATA
CTATGCTCGGATCGTCGAGCGCACAGCGACTTTGCGCCGACTGATCGGCGCTGGAACGGAAATCGTTCGCCTCGGCTTCG
ACGAACGAATCGAAGCGGACGAAGCGATCGAGCGAGCCGAGCGCCTCATCTTCGATGTCTCGCAGCGCCGAGCGAGTCGT
GACTTCGTTTCCGTCGGTCAAGTGCTCGAGCAGTTCTTCGAGAAGCTCGACTATATCCAGCAGCACCGCGGCGAAGTCAT
CGGCGTTCCCACCGGGTTCGCCGATCTGGACAAACTCACTGGAGGACTCCAACGCTCGGACTTGATCATCCTGGCGGCAC
GCCCCTCGGTGGGAAAGACGTCCTTCCAGCTCAGCATCGCCCATCATGCCGCCGTCAAGGCGGGCAAAGTGGTCGCCATC
TTTAGCCTCGAAATGTCGGCTGAACAACTCGTCCAGCGCCTCTTAGCTATGGAGACAGGAATCGATACGCACCGGCTCCG
TCTCGGCTATATCGACGAGCAAGAGTGGGACGCGATCTCGCGAGCCTTCGGTCGCTTGGCTGAAGCTAGGATCTTCATCG
ACGATACGCCCGGAATCAGCGTCATGGAACTCCGCAGCAAGGCTCGACGCCTGATGGCCGAGCACGGCCTCGACCTCGTG
ATCGTCGATTACCTTCAGCTGATGCACGCCCGCCGAGCTGAAAACCGCGTCCAGGAGATTTCGGAAATCTCGCGCGGACT
CAAAGCGCTGGCCCGTGAACTCAACGTGCCTGTCCTGGCCCTGTCGCAGCTCTCTCGAGCCGTTGAGACGCGAACGGATC
ATCGGCCGCTCCTGAGCGACCTGCGGGAGAGCGGTAGCCTGGAACAAGATGCGGATGTCGTCATCTTCATTTACCGGGAC
GAGATTTACAACCCCGACACCGACCGTCGGGGAATCGCCGAAATCATCGTGGCCAAGCACCGGAACGGACCGACGGATAC
CGTGCACCTTCGCTTCTTCGATCGAACAGCTCGCTTTGCCGATCTCGAACTCTATCGCGAACCCGGTGCGTGA

Upstream 100 bases:

>100_bases
CCGATTCACCTGGTCGGCAGTCTTCGCCCCACCGTGATCGTCGAGGTCGTACCAGAAGAAGGCGGTCGAGCTGGCGAGAA
CGAATGAGCAGGAGTGTGCC

Downstream 100 bases:

>100_bases
ACGAGGAGGCCGGATGGAGCGTGATGCGGTCGGTGTGCCCGGTTGTCTTCCCATCGATCTGGCCGAACGCTTGATCAGTC
AAGCGCGTGATGTCGCAACG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MTSTTAEAIERLPPHNIEAEQAVLGSLLIDRDAIIRVASFLRPDDFFRGSHSIIYQALLDLYNRRVPADFVTVVDELERT
GRLDDAGGVAYLTELLSVVPSAVHVEYYARIVERTATLRRLIGAGTEIVRLGFDERIEADEAIERAERLIFDVSQRRASR
DFVSVGQVLEQFFEKLDYIQQHRGEVIGVPTGFADLDKLTGGLQRSDLIILAARPSVGKTSFQLSIAHHAAVKAGKVVAI
FSLEMSAEQLVQRLLAMETGIDTHRLRLGYIDEQEWDAISRAFGRLAEARIFIDDTPGISVMELRSKARRLMAEHGLDLV
IVDYLQLMHARRAENRVQEISEISRGLKALARELNVPVLALSQLSRAVETRTDHRPLLSDLRESGSLEQDADVVIFIYRD
EIYNPDTDRRGIAEIIVAKHRNGPTDTVHLRFFDRTARFADLELYREPGA

Sequences:

>Translated_450_residues
MTSTTAEAIERLPPHNIEAEQAVLGSLLIDRDAIIRVASFLRPDDFFRGSHSIIYQALLDLYNRRVPADFVTVVDELERT
GRLDDAGGVAYLTELLSVVPSAVHVEYYARIVERTATLRRLIGAGTEIVRLGFDERIEADEAIERAERLIFDVSQRRASR
DFVSVGQVLEQFFEKLDYIQQHRGEVIGVPTGFADLDKLTGGLQRSDLIILAARPSVGKTSFQLSIAHHAAVKAGKVVAI
FSLEMSAEQLVQRLLAMETGIDTHRLRLGYIDEQEWDAISRAFGRLAEARIFIDDTPGISVMELRSKARRLMAEHGLDLV
IVDYLQLMHARRAENRVQEISEISRGLKALARELNVPVLALSQLSRAVETRTDHRPLLSDLRESGSLEQDADVVIFIYRD
EIYNPDTDRRGIAEIIVAKHRNGPTDTVHLRFFDRTARFADLELYREPGA
>Mature_449_residues
TSTTAEAIERLPPHNIEAEQAVLGSLLIDRDAIIRVASFLRPDDFFRGSHSIIYQALLDLYNRRVPADFVTVVDELERTG
RLDDAGGVAYLTELLSVVPSAVHVEYYARIVERTATLRRLIGAGTEIVRLGFDERIEADEAIERAERLIFDVSQRRASRD
FVSVGQVLEQFFEKLDYIQQHRGEVIGVPTGFADLDKLTGGLQRSDLIILAARPSVGKTSFQLSIAHHAAVKAGKVVAIF
SLEMSAEQLVQRLLAMETGIDTHRLRLGYIDEQEWDAISRAFGRLAEARIFIDDTPGISVMELRSKARRLMAEHGLDLVI
VDYLQLMHARRAENRVQEISEISRGLKALARELNVPVLALSQLSRAVETRTDHRPLLSDLRESGSLEQDADVVIFIYRDE
IYNPDTDRRGIAEIIVAKHRNGPTDTVHLRFFDRTARFADLELYREPGA

Specific function: Participates In Initiation And Elongation During Chromosome Replication; It Exhibits DNA-Dependent ATPase Activity And Contains Distinct Active Sites For ATP Binding, DNA Binding, And Interaction With DNAc Protein, Primase, And Other Prepriming Proteins.

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=436, Percent_Identity=46.7889908256881, Blast_Score=378, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50676; Mature: 50545

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTTAEAIERLPPHNIEAEQAVLGSLLIDRDAIIRVASFLRPDDFFRGSHSIIYQALLD
CCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHH
LYNRRVPADFVTVVDELERTGRLDDAGGVAYLTELLSVVPSAVHVEYYARIVERTATLRR
HHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LIGAGTEIVRLGFDERIEADEAIERAERLIFDVSQRRASRDFVSVGQVLEQFFEKLDYIQ
HHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QHRGEVIGVPTGFADLDKLTGGLQRSDLIILAARPSVGKTSFQLSIAHHAAVKAGKVVAI
HHCCCEEECCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEHHHHHHHCCCCEEEE
FSLEMSAEQLVQRLLAMETGIDTHRLRLGYIDEQEWDAISRAFGRLAEARIFIDDTPGIS
EEECCCHHHHHHHHHHHHCCCCHHEEEECCCCCHHHHHHHHHHHHHHHCEEEEECCCCCH
VMELRSKARRLMAEHGLDLVIVDYLQLMHARRAENRVQEISEISRGLKALARELNVPVLA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LSQLSRAVETRTDHRPLLSDLRESGSLEQDADVVIFIYRDEIYNPDTDRRGIAEIIVAKH
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHC
RNGPTDTVHLRFFDRTARFADLELYREPGA
CCCCCCEEEEEEECCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TSTTAEAIERLPPHNIEAEQAVLGSLLIDRDAIIRVASFLRPDDFFRGSHSIIYQALLD
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHH
LYNRRVPADFVTVVDELERTGRLDDAGGVAYLTELLSVVPSAVHVEYYARIVERTATLRR
HHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LIGAGTEIVRLGFDERIEADEAIERAERLIFDVSQRRASRDFVSVGQVLEQFFEKLDYIQ
HHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QHRGEVIGVPTGFADLDKLTGGLQRSDLIILAARPSVGKTSFQLSIAHHAAVKAGKVVAI
HHCCCEEECCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEHHHHHHHCCCCEEEE
FSLEMSAEQLVQRLLAMETGIDTHRLRLGYIDEQEWDAISRAFGRLAEARIFIDDTPGIS
EEECCCHHHHHHHHHHHHCCCCHHEEEECCCCCHHHHHHHHHHHHHHHCEEEEECCCCCH
VMELRSKARRLMAEHGLDLVIVDYLQLMHARRAENRVQEISEISRGLKALARELNVPVLA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LSQLSRAVETRTDHRPLLSDLRESGSLEQDADVVIFIYRDEIYNPDTDRRGIAEIIVAKH
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHC
RNGPTDTVHLRFFDRTARFADLELYREPGA
CCCCCCEEEEEEECCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1312531 [H]