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The map label for this gene is lexA [H]

Identifier: 221632683

GI number: 221632683

Start: 661242

End: 661886

Strand: Reverse

Name: lexA [H]

Synonym: trd_0666

Alternate gene names: 221632683

Gene position: 661886-661242 (Counterclockwise)

Preceding gene: 221632687

Following gene: 221632682

Centisome position: 33.04

GC content: 59.22

Gene sequence:

>645_bases
ATGGGCAAGCGACTCACCAAACGGCAACAGGAAATCCTCGAGTTCATTCGTCATTTCGTGGACGAGCGAGGCTATCCGCC
AACGGTGCGCGAGATCCAAGCGGGACTCGGTATCTCGTCCACCAGTGTTGTCGATTATAACCTGAATGTCCTCGAGTATC
TCCGCCTCATTCGCCGTAATCGAAACATTTCGCGCGGCATCGAGCTACTCGATCGCCGGCTCGGTCGCCACGATGCAGTC
GTTCGTGTCCCGCTCGTCGGCACGATCGCCGCGGGTCAGCCGCTACCTGTACTGGAGCCGATCGAAGTCGATGGCGCTGC
CGAATACGTTGAACTCGGTAGCCAACTCGTTGGGCATCCAGCGCGTGACCTGTTTGCCCTGCGCGTCAAGGGGCAATCGA
TGATCGACGCGCTGATCGACGATGGAGACATCGTCGTCTTGCGCCGGCAAGCCACCGCCGAAAACGGCGATACCGTCGCC
GCGTGGCTCCGACGGGAGCAGGAGACGACGCTCAAGCGCTTCTACCGCGAAGGAAATCTCGTGCGGTTGCAGCCAGCCAA
CGCCACCCTCCAACCGATCTATACGACGATCGATAACGTCGAAATTCAGGGAAAGTTGCTTTTGGTCATCCGGCGCTTGG
ACTGA

Upstream 100 bases:

>100_bases
ATGACCCTCGACCGCTAGACGATCCTCCCCTTGACACGAACGGATGTTCGCACCTATACTGGCACCGATGGTCGGGCCTG
ACAGAGAGCGACGGGGGCGA

Downstream 100 bases:

>100_bases
AGCGCCTGCGCCCGAAGCCGATATGTCCTGCCTGCACGCCTCCGGCGAGCACCGACGCCACGCCCCCGCGATCGTGCGCC
TCGCCGGCTGACATGGGCAA

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MGKRLTKRQQEILEFIRHFVDERGYPPTVREIQAGLGISSTSVVDYNLNVLEYLRLIRRNRNISRGIELLDRRLGRHDAV
VRVPLVGTIAAGQPLPVLEPIEVDGAAEYVELGSQLVGHPARDLFALRVKGQSMIDALIDDGDIVVLRRQATAENGDTVA
AWLRREQETTLKRFYREGNLVRLQPANATLQPIYTTIDNVEIQGKLLLVIRRLD

Sequences:

>Translated_214_residues
MGKRLTKRQQEILEFIRHFVDERGYPPTVREIQAGLGISSTSVVDYNLNVLEYLRLIRRNRNISRGIELLDRRLGRHDAV
VRVPLVGTIAAGQPLPVLEPIEVDGAAEYVELGSQLVGHPARDLFALRVKGQSMIDALIDDGDIVVLRRQATAENGDTVA
AWLRREQETTLKRFYREGNLVRLQPANATLQPIYTTIDNVEIQGKLLLVIRRLD
>Mature_213_residues
GKRLTKRQQEILEFIRHFVDERGYPPTVREIQAGLGISSTSVVDYNLNVLEYLRLIRRNRNISRGIELLDRRLGRHDAVV
RVPLVGTIAAGQPLPVLEPIEVDGAAEYVELGSQLVGHPARDLFALRVKGQSMIDALIDDGDIVVLRRQATAENGDTVAA
WLRREQETTLKRFYREGNLVRLQPANATLQPIYTTIDNVEIQGKLLLVIRRLD

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family [H]

Homologues:

Organism=Escherichia coli, GI1790476, Length=214, Percent_Identity=35.5140186915888, Blast_Score=98, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24 [H]

EC number: =3.4.21.88 [H]

Molecular weight: Translated: 24196; Mature: 24065

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKRLTKRQQEILEFIRHFVDERGYPPTVREIQAGLGISSTSVVDYNLNVLEYLRLIRRN
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHC
RNISRGIELLDRRLGRHDAVVRVPLVGTIAAGQPLPVLEPIEVDGAAEYVELGSQLVGHP
CCHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
ARDLFALRVKGQSMIDALIDDGDIVVLRRQATAENGDTVAAWLRREQETTLKRFYREGNL
CHHEEEEEECCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCE
VRLQPANATLQPIYTTIDNVEIQGKLLLVIRRLD
EEEECCCCCHHHHHHHHCCEEECCEEEEEEEECC
>Mature Secondary Structure 
GKRLTKRQQEILEFIRHFVDERGYPPTVREIQAGLGISSTSVVDYNLNVLEYLRLIRRN
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHC
RNISRGIELLDRRLGRHDAVVRVPLVGTIAAGQPLPVLEPIEVDGAAEYVELGSQLVGHP
CCHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
ARDLFALRVKGQSMIDALIDDGDIVVLRRQATAENGDTVAAWLRREQETTLKRFYREGNL
CHHEEEEEECCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCE
VRLQPANATLQPIYTTIDNVEIQGKLLLVIRRLD
EEEECCCCCHHHHHHHHCCEEECCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA