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The map label for this gene is pheS [H]

Identifier: 221632658

GI number: 221632658

Start: 631446

End: 632525

Strand: Reverse

Name: pheS [H]

Synonym: trd_0641

Alternate gene names: 221632658

Gene position: 632525-631446 (Counterclockwise)

Preceding gene: 221632660

Following gene: 221632657

Centisome position: 31.58

GC content: 60.46

Gene sequence:

>1080_bases
ATGAGCGCGACGACTCCGGAGCAGGAGGTATCCGTGAACACCGATCGGATCGAGGAACTGCGACAGCGTGCCAAACGAGA
ACTCCTGGCGATCACGACACAGGCTGCGCTCGCCGAATGGTACGCTCGATACCTCGGCCGACGCGGAGAGCTGACGCAAC
TTTTGCGAGCCTTGGGTACGCTGCCACCAGAACAACGGCCGATCATCGGGCGACTCGCCAATGAACTTCGGGATGAACTC
CAAGCCGCCTACGAGGAACGCGAGCGCGAGGTACGCGAGATCGAGCTCGCCGCCCGCATCGCGGCGGAAGCGATCGATGT
GACGCTTCCCGGCCGACGCCCGCGCATCGGGACACTCCACCCTGTTACGCAAATGATTCGCGAGGTAACCGACATCTTCG
CCCATCTCGGCTTCCAGGTGGTCGAGGGTCCTGAAGTGGAATATGGCTCTTACGCGTTCGATGCGCTCAATATTCCACCG
GAGCACCCGGCTCGCGACGTGTGGGACACGATCTTCATCGAGTCCGACCAAAAGGAGATCGTTCTGCGTCCGCATACTTC
GCCGATGCAGATCCGCGTGATGGAGCGCCGAAAGCCACCGGTTCGCGTCGTGGTGCCCGGTCGTTGTTACCGGTACGAAG
CGGTCGACGCGACGCATGAATGGCATTTCCACCAGATCGAGGGGCTCGCCGTCGATGAACAGATCACGATGGCTGATCTG
AAAGGGACGTTGGCCGAGTTCGCACGCCAGCTTTTCGGTAAGGAACGACGCGTCCGCTTTCGCTGCGATTTTTTCCCCTT
CGTCGAACCGGGGGTCGACTTCGCCATCGACTGCATGTTTTGCCGTGGCGAGGGCTGCCGAGTGTGCGGCCAAAGCGGGT
GGATCGAGATCCTGGGTGCAGGGATGGTTCACCCGCAGGTACTCCGCAACGTCGGATACGATCCTGACCGGTATACCGGT
TGGGCATTCGGCATGGGTGTCGAGAGACTGGTGATGCTTCGCTATGGTGTTCCTGATATCCGGTACTTCTACCAAGGCGA
TCTCCGCTTTCTTGCTCAGTTCGACCGCATACCGGTCTGA

Upstream 100 bases:

>100_bases
CGAAGTGTACCCGGCATCGGTGAACTCGACCCTCGCTTGACACGCGCCATCACGCATCCTTATGCTCAGGCCGCGTCCCG
ACGGGCGCGCCTGCGCGTGG

Downstream 100 bases:

>100_bases
TCGAGTCGAGAACGAAGGGCAGGCGCACCACCATGAAGGTTCCGATGCGCTGGCTCCAGGAATTGGTCGCGACCGACCGC
TCTGCAGAGGAAATCGCCGA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSATTPEQEVSVNTDRIEELRQRAKRELLAITTQAALAEWYARYLGRRGELTQLLRALGTLPPEQRPIIGRLANELRDEL
QAAYEEREREVREIELAARIAAEAIDVTLPGRRPRIGTLHPVTQMIREVTDIFAHLGFQVVEGPEVEYGSYAFDALNIPP
EHPARDVWDTIFIESDQKEIVLRPHTSPMQIRVMERRKPPVRVVVPGRCYRYEAVDATHEWHFHQIEGLAVDEQITMADL
KGTLAEFARQLFGKERRVRFRCDFFPFVEPGVDFAIDCMFCRGEGCRVCGQSGWIEILGAGMVHPQVLRNVGYDPDRYTG
WAFGMGVERLVMLRYGVPDIRYFYQGDLRFLAQFDRIPV

Sequences:

>Translated_359_residues
MSATTPEQEVSVNTDRIEELRQRAKRELLAITTQAALAEWYARYLGRRGELTQLLRALGTLPPEQRPIIGRLANELRDEL
QAAYEEREREVREIELAARIAAEAIDVTLPGRRPRIGTLHPVTQMIREVTDIFAHLGFQVVEGPEVEYGSYAFDALNIPP
EHPARDVWDTIFIESDQKEIVLRPHTSPMQIRVMERRKPPVRVVVPGRCYRYEAVDATHEWHFHQIEGLAVDEQITMADL
KGTLAEFARQLFGKERRVRFRCDFFPFVEPGVDFAIDCMFCRGEGCRVCGQSGWIEILGAGMVHPQVLRNVGYDPDRYTG
WAFGMGVERLVMLRYGVPDIRYFYQGDLRFLAQFDRIPV
>Mature_358_residues
SATTPEQEVSVNTDRIEELRQRAKRELLAITTQAALAEWYARYLGRRGELTQLLRALGTLPPEQRPIIGRLANELRDELQ
AAYEEREREVREIELAARIAAEAIDVTLPGRRPRIGTLHPVTQMIREVTDIFAHLGFQVVEGPEVEYGSYAFDALNIPPE
HPARDVWDTIFIESDQKEIVLRPHTSPMQIRVMERRKPPVRVVVPGRCYRYEAVDATHEWHFHQIEGLAVDEQITMADLK
GTLAEFARQLFGKERRVRFRCDFFPFVEPGVDFAIDCMFCRGEGCRVCGQSGWIEILGAGMVHPQVLRNVGYDPDRYTGW
AFGMGVERLVMLRYGVPDIRYFYQGDLRFLAQFDRIPV

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=275, Percent_Identity=29.0909090909091, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI5729820, Length=237, Percent_Identity=32.4894514767932, Blast_Score=105, Evalue=6e-23,
Organism=Escherichia coli, GI1788007, Length=338, Percent_Identity=47.3372781065089, Blast_Score=313, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI32563657, Length=276, Percent_Identity=28.6231884057971, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17508957, Length=276, Percent_Identity=28.6231884057971, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI32566635, Length=259, Percent_Identity=30.5019305019305, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321087, Length=267, Percent_Identity=31.0861423220974, Blast_Score=122, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6325304, Length=103, Percent_Identity=34.9514563106796, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI18858079, Length=275, Percent_Identity=29.0909090909091, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17137424, Length=250, Percent_Identity=27.2, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 41331; Mature: 41200

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATTPEQEVSVNTDRIEELRQRAKRELLAITTQAALAEWYARYLGRRGELTQLLRALGT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
LPPEQRPIIGRLANELRDELQAAYEEREREVREIELAARIAAEAIDVTLPGRRPRIGTLH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHH
PVTQMIREVTDIFAHLGFQVVEGPEVEYGSYAFDALNIPPEHPARDVWDTIFIESDQKEI
HHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHEEEECCCCEE
VLRPHTSPMQIRVMERRKPPVRVVVPGRCYRYEAVDATHEWHFHQIEGLAVDEQITMADL
EECCCCCCCEEEEECCCCCCEEEEECCCEEEEEECCCCCCCCCEEECCEEECCHHHHHHH
KGTLAEFARQLFGKERRVRFRCDFFPFVEPGVDFAIDCMFCRGEGCRVCGQSGWIEILGA
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCEECCCCCCEEEEEC
GMVHPQVLRNVGYDPDRYTGWAFGMGVERLVMLRYGVPDIRYFYQGDLRFLAQFDRIPV
CCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SATTPEQEVSVNTDRIEELRQRAKRELLAITTQAALAEWYARYLGRRGELTQLLRALGT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
LPPEQRPIIGRLANELRDELQAAYEEREREVREIELAARIAAEAIDVTLPGRRPRIGTLH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHH
PVTQMIREVTDIFAHLGFQVVEGPEVEYGSYAFDALNIPPEHPARDVWDTIFIESDQKEI
HHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHEEEECCCCEE
VLRPHTSPMQIRVMERRKPPVRVVVPGRCYRYEAVDATHEWHFHQIEGLAVDEQITMADL
EECCCCCCCEEEEECCCCCCEEEEECCCEEEEEECCCCCCCCCEEECCEEECCHHHHHHH
KGTLAEFARQLFGKERRVRFRCDFFPFVEPGVDFAIDCMFCRGEGCRVCGQSGWIEILGA
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCEECCCCCCEEEEEC
GMVHPQVLRNVGYDPDRYTGWAFGMGVERLVMLRYGVPDIRYFYQGDLRFLAQFDRIPV
CCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA