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The map label for this gene is ilvB [H]

Identifier: 221632644

GI number: 221632644

Start: 617499

End: 619271

Strand: Reverse

Name: ilvB [H]

Synonym: trd_0627

Alternate gene names: 221632644

Gene position: 619271-617499 (Counterclockwise)

Preceding gene: 221632646

Following gene: 221632643

Centisome position: 30.92

GC content: 61.25

Gene sequence:

>1773_bases
ATGTATCGACGACTGTCGAGAAGGGAGGGCGAACTGATGGCGATCGAGACGAAGGAACGACGAACGGTCGAGGAAGAGCC
ACAACGCGAACCGCAACTCGGTGCGCACTTGGTGATGCAGGCGCTGGTTCGCGAAGGGGTGGAAGTGATCTTCGGTTATC
CCGGTGGGGCGATCCTCCCCGTCTACGATGCATTGCCGAGTTATCCGATCCATCATGTGCTCGTCCGTCATGAACAGTCG
GCAGCGTTTGCCGCTGATGGCTACGCTCGGGCAACCGGCAAAGTCGGCGTTTGTATGGCAACCTCTGGCCCCGGCGCCAC
CAACCTCGTCACTGGGATCGCTAACGCGATGATGGATTCCATTCCCATCGTGGCGATCACGGGACAAGTGCCACAAAGCG
TGATCGGGATGGACGCATTTCAAGAGACGGACATCACGGGGATCACCCTGCCCATCACCAAGTACAACATGGTCATCCGG
AGTGTCGAAGAGATCGGGCCAGCCATTCAGAAAGCCTTTTACCTCGCGCGCAGCGGTCGGCCAGGGCCGGTTCTGATCGA
TATTCCCAAGGACGTGCAGCTGGCCAAGGGGTATTTGGCCCCCAGACAGGAATTGCATCTGCCTGGTTACCGACCGACCA
TCGTGCCGCATCGCCGACAAATCCGGCGGGCAGCGGAACTCATCCGGCAAGCCGAGCGCCCGCTCATCCTTGCCGGTCAC
GGCATCATCATCAGCGGTGCCACCGAAGAACTCGTTCGGTTCGCCGAACGGACACAGATTCCCGTCGGCTTGACACTCCT
CGGGATCAGCGGTTTTCCCGCCTCTCACCCGCTCTGTCTCGGCATGGTCGGCATGCACGGACTCGCGCACGCCAATCGAG
CGATCCACGAAGCGGACCTCATCATCGGCATCGGCATGCGTTTCGATGACCGCGTGACGGGTCGACCGAGCGAATTCGCG
CCACACGCGAAGATCATCCATATCGATATCGACCCTGCGGAAATCGGCAAAGTCCTCAAGACCGAAGTGCCGGTGGTGGG
CGATGCCCGCGAGGCCCTGCGGTTGTTGCTCGAGGAACTCGAACCGCTCGAGCACGACGACTGGCTCAGCGCGATCCGCA
GTTGGTACCGACCGATCCGCACCGAGCCCTCGGGACCCGACGGAGCTCTGCAGCCGCAATACGTGATCGCCCGGCTCCAT
GCCCTAACCGAGGGAAAGGCGATCGTGACGACCGACGTCGGTCAGCATCAGATGTGGACGGCCCAGCTCTACCGCTGCGA
CATTCCCAAACAGTGGATCAGCTCGGGCGGATTGGGTGCTATGGGCTTCGGTGTGCCGTCAGCGATGGGCGCAGCGATCG
GGCGACCGGATGCCGAAGTGTGGGCGGTTGTCGGCGACGGCGGCGTCCAGATGACGCTCAACGAGTTCGCCACGCTCGTG
GACGAGCAACTCAACGTCAAAGTGGCCATTATCAACAACGGGTATCTCGGTATGGTACGGCAATGGCAACAGTTGTTCCA
CAACCGCAATTACTCCGAGACGCCCATCTCCAGCCCGGACTACGTTTTGCTGGGCCAGGCCTACCGCATGGCGGCCCGTC
GCGTGACACGACCTGCTGAGGTCGACGACGCGATCACTTGGGCACGCTCGTTCCGCGGCCCAGCTCTCATCGAGTTCGTG
GTGAACCAGGAAGAGAACGTGTTCCCGATGATTCCATCGGGGGGATCGTTCCGCGACATGATCGACGATTACCGGATCGA
GGGGGAATGGTGA

Upstream 100 bases:

>100_bases
TACTTACGAGCCTGCTGCTGGTGGGAGGCTGAGCGAGCGCGATCCAATAACCAACAAGGAACGAAGCGTCTCGCGAGCCT
CCCCCAGCGGGAGGCTCGCG

Downstream 100 bases:

>100_bases
GCGTGCGGAGCCATACGCTGGTCGTCTTGGTCGAAGACAAGCCGGGTGTCATGAACCGGATCGTCAGCCTGTTCCGCCAG
CGCGGATTCAATATCGACTC

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MYRRLSRREGELMAIETKERRTVEEEPQREPQLGAHLVMQALVREGVEVIFGYPGGAILPVYDALPSYPIHHVLVRHEQS
AAFAADGYARATGKVGVCMATSGPGATNLVTGIANAMMDSIPIVAITGQVPQSVIGMDAFQETDITGITLPITKYNMVIR
SVEEIGPAIQKAFYLARSGRPGPVLIDIPKDVQLAKGYLAPRQELHLPGYRPTIVPHRRQIRRAAELIRQAERPLILAGH
GIIISGATEELVRFAERTQIPVGLTLLGISGFPASHPLCLGMVGMHGLAHANRAIHEADLIIGIGMRFDDRVTGRPSEFA
PHAKIIHIDIDPAEIGKVLKTEVPVVGDAREALRLLLEELEPLEHDDWLSAIRSWYRPIRTEPSGPDGALQPQYVIARLH
ALTEGKAIVTTDVGQHQMWTAQLYRCDIPKQWISSGGLGAMGFGVPSAMGAAIGRPDAEVWAVVGDGGVQMTLNEFATLV
DEQLNVKVAIINNGYLGMVRQWQQLFHNRNYSETPISSPDYVLLGQAYRMAARRVTRPAEVDDAITWARSFRGPALIEFV
VNQEENVFPMIPSGGSFRDMIDDYRIEGEW

Sequences:

>Translated_590_residues
MYRRLSRREGELMAIETKERRTVEEEPQREPQLGAHLVMQALVREGVEVIFGYPGGAILPVYDALPSYPIHHVLVRHEQS
AAFAADGYARATGKVGVCMATSGPGATNLVTGIANAMMDSIPIVAITGQVPQSVIGMDAFQETDITGITLPITKYNMVIR
SVEEIGPAIQKAFYLARSGRPGPVLIDIPKDVQLAKGYLAPRQELHLPGYRPTIVPHRRQIRRAAELIRQAERPLILAGH
GIIISGATEELVRFAERTQIPVGLTLLGISGFPASHPLCLGMVGMHGLAHANRAIHEADLIIGIGMRFDDRVTGRPSEFA
PHAKIIHIDIDPAEIGKVLKTEVPVVGDAREALRLLLEELEPLEHDDWLSAIRSWYRPIRTEPSGPDGALQPQYVIARLH
ALTEGKAIVTTDVGQHQMWTAQLYRCDIPKQWISSGGLGAMGFGVPSAMGAAIGRPDAEVWAVVGDGGVQMTLNEFATLV
DEQLNVKVAIINNGYLGMVRQWQQLFHNRNYSETPISSPDYVLLGQAYRMAARRVTRPAEVDDAITWARSFRGPALIEFV
VNQEENVFPMIPSGGSFRDMIDDYRIEGEW
>Mature_590_residues
MYRRLSRREGELMAIETKERRTVEEEPQREPQLGAHLVMQALVREGVEVIFGYPGGAILPVYDALPSYPIHHVLVRHEQS
AAFAADGYARATGKVGVCMATSGPGATNLVTGIANAMMDSIPIVAITGQVPQSVIGMDAFQETDITGITLPITKYNMVIR
SVEEIGPAIQKAFYLARSGRPGPVLIDIPKDVQLAKGYLAPRQELHLPGYRPTIVPHRRQIRRAAELIRQAERPLILAGH
GIIISGATEELVRFAERTQIPVGLTLLGISGFPASHPLCLGMVGMHGLAHANRAIHEADLIIGIGMRFDDRVTGRPSEFA
PHAKIIHIDIDPAEIGKVLKTEVPVVGDAREALRLLLEELEPLEHDDWLSAIRSWYRPIRTEPSGPDGALQPQYVIARLH
ALTEGKAIVTTDVGQHQMWTAQLYRCDIPKQWISSGGLGAMGFGVPSAMGAAIGRPDAEVWAVVGDGGVQMTLNEFATLV
DEQLNVKVAIINNGYLGMVRQWQQLFHNRNYSETPISSPDYVLLGQAYRMAARRVTRPAEVDDAITWARSFRGPALIEFV
VNQEENVFPMIPSGGSFRDMIDDYRIEGEW

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=508, Percent_Identity=28.9370078740157, Blast_Score=170, Evalue=4e-42,
Organism=Homo sapiens, GI93004078, Length=512, Percent_Identity=27.734375, Blast_Score=166, Evalue=6e-41,
Organism=Escherichia coli, GI87081685, Length=563, Percent_Identity=43.5168738898757, Blast_Score=477, Evalue=1e-136,
Organism=Escherichia coli, GI1790104, Length=550, Percent_Identity=42.7272727272727, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI1786717, Length=556, Percent_Identity=35.2517985611511, Blast_Score=328, Evalue=6e-91,
Organism=Escherichia coli, GI1787096, Length=547, Percent_Identity=30.164533820841, Blast_Score=218, Evalue=9e-58,
Organism=Escherichia coli, GI1788716, Length=561, Percent_Identity=26.9162210338681, Blast_Score=154, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17542570, Length=603, Percent_Identity=25.8706467661692, Blast_Score=155, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI17531299, Length=493, Percent_Identity=28.6004056795132, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=493, Percent_Identity=28.6004056795132, Blast_Score=145, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6323755, Length=573, Percent_Identity=42.0593368237347, Blast_Score=431, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6320816, Length=474, Percent_Identity=24.4725738396624, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6323163, Length=506, Percent_Identity=24.5059288537549, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922626, Length=479, Percent_Identity=26.5135699373695, Blast_Score=161, Evalue=1e-39,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64879; Mature: 64879

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRRLSRREGELMAIETKERRTVEEEPQREPQLGAHLVMQALVREGVEVIFGYPGGAILP
CCCCCCCCCCCEEEEECHHHCCCHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCEEE
VYDALPSYPIHHVLVRHEQSAAFAADGYARATGKVGVCMATSGPGATNLVTGIANAMMDS
HHHCCCCCCHHHHHEECCCCCCEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHC
IPIVAITGQVPQSVIGMDAFQETDITGITLPITKYNMVIRSVEEIGPAIQKAFYLARSGR
CCEEEEECCCCHHHHCCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PGPVLIDIPKDVQLAKGYLAPRQELHLPGYRPTIVPHRRQIRRAAELIRQAERPLILAGH
CCCEEEECCCCHHHHHCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECC
GIIISGATEELVRFAERTQIPVGLTLLGISGFPASHPLCLGMVGMHGLAHANRAIHEADL
CEEEECCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCE
IIGIGMRFDDRVTGRPSEFAPHAKIIHIDIDPAEIGKVLKTEVPVVGDAREALRLLLEEL
EEEECCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHC
EPLEHDDWLSAIRSWYRPIRTEPSGPDGALQPQYVIARLHALTEGKAIVTTDVGQHQMWT
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHE
AQLYRCDIPKQWISSGGLGAMGFGVPSAMGAAIGRPDAEVWAVVGDGGVQMTLNEFATLV
EHHEECCCCHHHHCCCCCCEECCCCHHHHHHHCCCCCCEEEEEEECCCEEEEHHHHHHHH
DEQLNVKVAIINNGYLGMVRQWQQLFHNRNYSETPISSPDYVLLGQAYRMAARRVTRPAE
HHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHHHHHHHHCCCCCC
VDDAITWARSFRGPALIEFVVNQEENVFPMIPSGGSFRDMIDDYRIEGEW
HHHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCCCHHHHHHHCEECCCC
>Mature Secondary Structure
MYRRLSRREGELMAIETKERRTVEEEPQREPQLGAHLVMQALVREGVEVIFGYPGGAILP
CCCCCCCCCCCEEEEECHHHCCCHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCEEE
VYDALPSYPIHHVLVRHEQSAAFAADGYARATGKVGVCMATSGPGATNLVTGIANAMMDS
HHHCCCCCCHHHHHEECCCCCCEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHC
IPIVAITGQVPQSVIGMDAFQETDITGITLPITKYNMVIRSVEEIGPAIQKAFYLARSGR
CCEEEEECCCCHHHHCCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PGPVLIDIPKDVQLAKGYLAPRQELHLPGYRPTIVPHRRQIRRAAELIRQAERPLILAGH
CCCEEEECCCCHHHHHCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECC
GIIISGATEELVRFAERTQIPVGLTLLGISGFPASHPLCLGMVGMHGLAHANRAIHEADL
CEEEECCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCE
IIGIGMRFDDRVTGRPSEFAPHAKIIHIDIDPAEIGKVLKTEVPVVGDAREALRLLLEEL
EEEECCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHC
EPLEHDDWLSAIRSWYRPIRTEPSGPDGALQPQYVIARLHALTEGKAIVTTDVGQHQMWT
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHE
AQLYRCDIPKQWISSGGLGAMGFGVPSAMGAAIGRPDAEVWAVVGDGGVQMTLNEFATLV
EHHEECCCCHHHHCCCCCCEECCCCHHHHHHHCCCCCCEEEEEEECCCEEEEHHHHHHHH
DEQLNVKVAIINNGYLGMVRQWQQLFHNRNYSETPISSPDYVLLGQAYRMAARRVTRPAE
HHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHHHHHHHHCCCCCC
VDDAITWARSFRGPALIEFVVNQEENVFPMIPSGGSFRDMIDDYRIEGEW
HHHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCCCHHHHHHHCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]