Definition Thermomicrobium roseum DSM 5159 chromosome, complete genome.
Accession NC_011959
Length 2,003,006

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The map label for this gene is pnp

Identifier: 221632640

GI number: 221632640

Start: 612948

End: 615320

Strand: Reverse

Name: pnp

Synonym: trd_0623

Alternate gene names: 221632640

Gene position: 615320-612948 (Counterclockwise)

Preceding gene: 221632641

Following gene: 221632638

Centisome position: 30.72

GC content: 60.72

Gene sequence:

>2373_bases
ATGCCACCGATCATCCATGAGCGCTCCATCGAGATTGCCGGTCGTCTCCTGACGATCGAAACTGGCCGGGTAGCGGAGCA
AGCTGATGGTGCCGTCCTCGTGCGCTACGGCGAAACCGTCGTCTTGACGACGGTGGTCGGCGCAAAACAGCCGGTCGAAG
GAATCGACTTCTTCCCCTTGACGGTCGAGTACGAAGAGAAAATGTACGCCGCTGGCAAAATACCCGGCGGCTTCTTCAGA
CGCGAGGGTAAGCCATCCGAGGCCGCGATTCTCGCCGCCCGGCTCACCGACCGCCCGATCCGTCCCCTGTTTCCCAAAGG
CTATCGGAACGAGGTCCAAGTCATTTCGACGGTTCTCTCTGCCGATCAAGAGAACGAACCAGATGTCCTCTCCATCATCG
GTGCGTCAGCTGCCCTCACTTTGTCCGACATCCCTTGGTACGGGCCAGTCGGTGCCGTCCGAATCGGCGAACTCGATGGT
GAGTTAGTGATCAACCCAACGAGCCATCAGCTCCTCGAAAGCCGGATGGACATCGTTGTCGCTGGCACGGCCGATGCCAT
CCTGATGGTCGAAGGGCAGGCGAACGAGATCTCGGAAGACCGCTTTATCGAAGCTGTTGTCCGAGCGCATCAGGAGATCA
AGCGGATCGTTGCTGTGCAGCTCGAACTCCAGGCTGTGGCTGGCAAACCGAAACGAGAATTCGTGCCACCGCAAGAAAAT
GTCGAGCTCAAACAGCAGATCGCCGACTATCTCGGTGATCGGCTGCGTGAGGCTGTCTTCAATCCAGACAAGACACTGCG
TGTCCAGGCGACCGCTGCACTGCGCGAGGAAGTCATCGCTCACTTCGTTCCGAACGAACCACTGGCCATTGGCACTCCCC
AGTCTGGTCTCCCGACCGCAAAGGAAGTGGGCGACTTGTTCGATTCCCTGGTCAAGGAACTGGTGCGCCGCACGATCCTC
GAACAGGGCGAACGCCCCGATGGTCGCAAGCCGGACGAAATCCGCGAGATCTGGATCCAGGTCGGTCTGCTTCCGCGACC
GCATGGCTCTGCACTGTTCACGCGCGGCCAAACGCAAGTCCTCACGGTCTGTACGCTGGGAACCAAAGAAGAGGAACAAT
TCCTCGATTCCCTGGGAATCGAGGAAACCAAGCGCTACATGCACCACTACAACTTTCCACCCTTCAGCACTGGTGAAATC
CGGCGGCTGCGTGGTCCGAGCAGGCGAGACATCGGCCACGGCGCTCTGGCCGAGCGCGCTCTTCTGGCTGTCCTACCGAG
CGAAGACGAATTTCCTTACACGATGCGACTCGTCTCGGAAGTTCTCAGCTCCAACGGCTCGACCTCGATGGCCAGCGTCT
GCGGCTCCAGTCTCGCCTTGATGGATGCCGGTGTCCCTATCCGCAAGCCGGTCGCCGGCGTCGCGATGGGACTCGTTACC
GATCAGACGACCGGCCGCTACACCATTCTCACTGACATCCAGGGAATCGAAGATGCCCTCGGCGACATGGACTTCAAGGT
TGCAGGCACGCGCGACGGAATCACCGCCATTCAAATGGATATCAAGGTGATGGGAATCACTCCCGAGATCATGCGCGACG
CGCTCGAGCAAGCGCGCCGCGGTCGTCTCTTCATCCTCGACAAGATGTCCGAGGTGATCGACGCCCCCCGTCCGGAGATG
TCGCCGTATGCACCACGCATCCTGCGGATCAAGATCAAGCCCGAACAGATCGGCGAAGTGATCGGGCCGGGCGGACGAGT
CATCCGCGCCATCCAGGAGCAAACCGGAACCAAGATCTCGATCGAAGAGGATGGCACTGTCTTCATCAGTGCTGCCAACG
AGGACGCTGCGCGACGCGCTGTTCGGGAAATCGAGCGGCTCACCCGGGTGCCGGAGGTCGGCGAAATCTTCTACGGCCGC
GTGGTCACCATCATCCCCTCCGGCGCCTTCGTCGAGATCCTTCCCGGCAAGGACGGCTTCCTCCACATTTCAGAGATCGC
TCCCGAGCGGGTCCGCTCAGTGGAGGATGTCCTCAAGGTCGGACAAGAGATCAACGTCATGGTCATCGGTGTCCGCCCGG
ACGGCAAGATCAACCTTTCCCGCAAAGCTCTCCTGGAGAAGGAAGCGGCAGAACGTGCCGCCACAGCACAAGCACCAGCC
GATGGGCGAAGCCACCAGCCGCGAGCACCTCAGCGGCCGAGTGGAACGGCTCAGCCTGAGCGACGCCCTGGTCCTCCAAC
ACCCCGGCGACCGGAACAGCGTGGTCCGAGCCGCCCACCACGACCACAAGCTCAGCGTTCCACCCCACCACCTGGTCAAT
ACCGCATCGGAGACCGCCTCAAGGAACTTCTGGGGGAAGACGAACCGAATTGA

Upstream 100 bases:

>100_bases
ATGGCCCAGGGTGTTGTCCCCTGGGCCATCTCATTGCAGCGAGCGAGAGCACGACTGTGCGTTGTCACGATACGATCACG
AACGGAGGAGAGTGGGAGCC

Downstream 100 bases:

>100_bases
CCCACCTTCCCACGCCGGGCTCGTCGTGCCTACTCGCCCAGCAATGACGAGCCCGGCGCGAGTATCCGCTCACTCGGCCA
CCAGCGAGCGACGTTCGGGC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 790; Mature: 789

Protein sequence:

>790_residues
MPPIIHERSIEIAGRLLTIETGRVAEQADGAVLVRYGETVVLTTVVGAKQPVEGIDFFPLTVEYEEKMYAAGKIPGGFFR
REGKPSEAAILAARLTDRPIRPLFPKGYRNEVQVISTVLSADQENEPDVLSIIGASAALTLSDIPWYGPVGAVRIGELDG
ELVINPTSHQLLESRMDIVVAGTADAILMVEGQANEISEDRFIEAVVRAHQEIKRIVAVQLELQAVAGKPKREFVPPQEN
VELKQQIADYLGDRLREAVFNPDKTLRVQATAALREEVIAHFVPNEPLAIGTPQSGLPTAKEVGDLFDSLVKELVRRTIL
EQGERPDGRKPDEIREIWIQVGLLPRPHGSALFTRGQTQVLTVCTLGTKEEEQFLDSLGIEETKRYMHHYNFPPFSTGEI
RRLRGPSRRDIGHGALAERALLAVLPSEDEFPYTMRLVSEVLSSNGSTSMASVCGSSLALMDAGVPIRKPVAGVAMGLVT
DQTTGRYTILTDIQGIEDALGDMDFKVAGTRDGITAIQMDIKVMGITPEIMRDALEQARRGRLFILDKMSEVIDAPRPEM
SPYAPRILRIKIKPEQIGEVIGPGGRVIRAIQEQTGTKISIEEDGTVFISAANEDAARRAVREIERLTRVPEVGEIFYGR
VVTIIPSGAFVEILPGKDGFLHISEIAPERVRSVEDVLKVGQEINVMVIGVRPDGKINLSRKALLEKEAAERAATAQAPA
DGRSHQPRAPQRPSGTAQPERRPGPPTPRRPEQRGPSRPPRPQAQRSTPPPGQYRIGDRLKELLGEDEPN

Sequences:

>Translated_790_residues
MPPIIHERSIEIAGRLLTIETGRVAEQADGAVLVRYGETVVLTTVVGAKQPVEGIDFFPLTVEYEEKMYAAGKIPGGFFR
REGKPSEAAILAARLTDRPIRPLFPKGYRNEVQVISTVLSADQENEPDVLSIIGASAALTLSDIPWYGPVGAVRIGELDG
ELVINPTSHQLLESRMDIVVAGTADAILMVEGQANEISEDRFIEAVVRAHQEIKRIVAVQLELQAVAGKPKREFVPPQEN
VELKQQIADYLGDRLREAVFNPDKTLRVQATAALREEVIAHFVPNEPLAIGTPQSGLPTAKEVGDLFDSLVKELVRRTIL
EQGERPDGRKPDEIREIWIQVGLLPRPHGSALFTRGQTQVLTVCTLGTKEEEQFLDSLGIEETKRYMHHYNFPPFSTGEI
RRLRGPSRRDIGHGALAERALLAVLPSEDEFPYTMRLVSEVLSSNGSTSMASVCGSSLALMDAGVPIRKPVAGVAMGLVT
DQTTGRYTILTDIQGIEDALGDMDFKVAGTRDGITAIQMDIKVMGITPEIMRDALEQARRGRLFILDKMSEVIDAPRPEM
SPYAPRILRIKIKPEQIGEVIGPGGRVIRAIQEQTGTKISIEEDGTVFISAANEDAARRAVREIERLTRVPEVGEIFYGR
VVTIIPSGAFVEILPGKDGFLHISEIAPERVRSVEDVLKVGQEINVMVIGVRPDGKINLSRKALLEKEAAERAATAQAPA
DGRSHQPRAPQRPSGTAQPERRPGPPTPRRPEQRGPSRPPRPQAQRSTPPPGQYRIGDRLKELLGEDEPN
>Mature_789_residues
PPIIHERSIEIAGRLLTIETGRVAEQADGAVLVRYGETVVLTTVVGAKQPVEGIDFFPLTVEYEEKMYAAGKIPGGFFRR
EGKPSEAAILAARLTDRPIRPLFPKGYRNEVQVISTVLSADQENEPDVLSIIGASAALTLSDIPWYGPVGAVRIGELDGE
LVINPTSHQLLESRMDIVVAGTADAILMVEGQANEISEDRFIEAVVRAHQEIKRIVAVQLELQAVAGKPKREFVPPQENV
ELKQQIADYLGDRLREAVFNPDKTLRVQATAALREEVIAHFVPNEPLAIGTPQSGLPTAKEVGDLFDSLVKELVRRTILE
QGERPDGRKPDEIREIWIQVGLLPRPHGSALFTRGQTQVLTVCTLGTKEEEQFLDSLGIEETKRYMHHYNFPPFSTGEIR
RLRGPSRRDIGHGALAERALLAVLPSEDEFPYTMRLVSEVLSSNGSTSMASVCGSSLALMDAGVPIRKPVAGVAMGLVTD
QTTGRYTILTDIQGIEDALGDMDFKVAGTRDGITAIQMDIKVMGITPEIMRDALEQARRGRLFILDKMSEVIDAPRPEMS
PYAPRILRIKIKPEQIGEVIGPGGRVIRAIQEQTGTKISIEEDGTVFISAANEDAARRAVREIERLTRVPEVGEIFYGRV
VTIIPSGAFVEILPGKDGFLHISEIAPERVRSVEDVLKVGQEINVMVIGVRPDGKINLSRKALLEKEAAERAATAQAPAD
GRSHQPRAPQRPSGTAQPERRPGPPTPRRPEQRGPSRPPRPQAQRSTPPPGQYRIGDRLKELLGEDEPN

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=727, Percent_Identity=39.7524071526823, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI9506689, Length=231, Percent_Identity=25.974025974026, Blast_Score=70, Evalue=7e-12,
Organism=Escherichia coli, GI145693187, Length=708, Percent_Identity=50.2824858757062, Blast_Score=668, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=722, Percent_Identity=35.1800554016621, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI281362905, Length=719, Percent_Identity=42.0027816411683, Blast_Score=481, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24651641, Length=719, Percent_Identity=42.0027816411683, Blast_Score=481, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24651643, Length=719, Percent_Identity=42.0027816411683, Blast_Score=481, Evalue=1e-135,
Organism=Drosophila melanogaster, GI161079377, Length=663, Percent_Identity=42.0814479638009, Blast_Score=441, Evalue=1e-124,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_THERP (B9KYR9)

Other databases:

- EMBL:   CP001275
- RefSeq:   YP_002521861.1
- GeneID:   7354872
- GenomeReviews:   CP001275_GR
- KEGG:   tro:trd_0623
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 86690; Mature: 86558

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MPPIIHERSIEIAGRLLTIETGRVAEQADGAVLVRYGETVVLTTVVGAKQPVEGIDFFPL
CCCCCCCCCCEECCEEEEEECCCCHHHCCCEEEEEECCEEEEEEECCCCCCCCCCCEEEE
TVEYEEKMYAAGKIPGGFFRREGKPSEAAILAARLTDRPIRPLFPKGYRNEVQVISTVLS
EEEHHHHHHCCCCCCCHHHCCCCCCCHHHEEEEHHCCCCCCCCCCCCCCHHHHHHHHHHH
ADQENEPDVLSIIGASAALTLSDIPWYGPVGAVRIGELDGELVINPTSHQLLESRMDIVV
CCCCCCCCCEEEECCCCEEEECCCCCCCCCCCEEEECCCCCEEECCCHHHHHHCCCCEEE
AGTADAILMVEGQANEISEDRFIEAVVRAHQEIKRIVAVQLELQAVAGKPKREFVPPQEN
ECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEHHHCCCCCCCCCCCCCC
VELKQQIADYLGDRLREAVFNPDKTLRVQATAALREEVIAHFVPNEPLAIGTPQSGLPTA
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCCCEEECCCCCCCCCH
KEVGDLFDSLVKELVRRTILEQGERPDGRKPDEIREIWIQVGLLPRPHGSALFTRGQTQV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCEE
LTVCTLGTKEEEQFLDSLGIEETKRYMHHYNFPPFSTGEIRRLRGPSRRDIGHGALAERA
EEEEECCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHH
LLAVLPSEDEFPYTMRLVSEVLSSNGSTSMASVCGSSLALMDAGVPIRKPVAGVAMGLVT
HEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCEEHCCCCCCCCCHHHHHHHHEE
DQTTGRYTILTDIQGIEDALGDMDFKVAGTRDGITAIQMDIKVMGITPEIMRDALEQARR
CCCCCCEEEEECCHHHHHHHCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHC
GRLFILDKMSEVIDAPRPEMSPYAPRILRIKIKPEQIGEVIGPGGRVIRAIQEQTGTKIS
CCEEEEHHHHHHHCCCCCCCCCCCCEEEEEEECHHHHHHHHCCCHHHHHHHHHHCCCEEE
IEEDGTVFISAANEDAARRAVREIERLTRVPEVGEIFYGRVVTIIPSGAFVEILPGKDGF
EECCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHCEEEEEECCCCEEEEECCCCCC
LHISEIAPERVRSVEDVLKVGQEINVMVIGVRPDGKINLSRKALLEKEAAERAATAQAPA
EEHHHCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCC
DGRSHQPRAPQRPSGTAQPERRPGPPTPRRPEQRGPSRPPRPQAQRSTPPPGQYRIGDRL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
KELLGEDEPN
HHHHCCCCCC
>Mature Secondary Structure 
PPIIHERSIEIAGRLLTIETGRVAEQADGAVLVRYGETVVLTTVVGAKQPVEGIDFFPL
CCCCCCCCCEECCEEEEEECCCCHHHCCCEEEEEECCEEEEEEECCCCCCCCCCCEEEE
TVEYEEKMYAAGKIPGGFFRREGKPSEAAILAARLTDRPIRPLFPKGYRNEVQVISTVLS
EEEHHHHHHCCCCCCCHHHCCCCCCCHHHEEEEHHCCCCCCCCCCCCCCHHHHHHHHHHH
ADQENEPDVLSIIGASAALTLSDIPWYGPVGAVRIGELDGELVINPTSHQLLESRMDIVV
CCCCCCCCCEEEECCCCEEEECCCCCCCCCCCEEEECCCCCEEECCCHHHHHHCCCCEEE
AGTADAILMVEGQANEISEDRFIEAVVRAHQEIKRIVAVQLELQAVAGKPKREFVPPQEN
ECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEHHHCCCCCCCCCCCCCC
VELKQQIADYLGDRLREAVFNPDKTLRVQATAALREEVIAHFVPNEPLAIGTPQSGLPTA
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCCCEEECCCCCCCCCH
KEVGDLFDSLVKELVRRTILEQGERPDGRKPDEIREIWIQVGLLPRPHGSALFTRGQTQV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCEE
LTVCTLGTKEEEQFLDSLGIEETKRYMHHYNFPPFSTGEIRRLRGPSRRDIGHGALAERA
EEEEECCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHH
LLAVLPSEDEFPYTMRLVSEVLSSNGSTSMASVCGSSLALMDAGVPIRKPVAGVAMGLVT
HEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCEEHCCCCCCCCCHHHHHHHHEE
DQTTGRYTILTDIQGIEDALGDMDFKVAGTRDGITAIQMDIKVMGITPEIMRDALEQARR
CCCCCCEEEEECCHHHHHHHCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHC
GRLFILDKMSEVIDAPRPEMSPYAPRILRIKIKPEQIGEVIGPGGRVIRAIQEQTGTKIS
CCEEEEHHHHHHHCCCCCCCCCCCCEEEEEEECHHHHHHHHCCCHHHHHHHHHHCCCEEE
IEEDGTVFISAANEDAARRAVREIERLTRVPEVGEIFYGRVVTIIPSGAFVEILPGKDGF
EECCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHCEEEEEECCCCEEEEECCCCCC
LHISEIAPERVRSVEDVLKVGQEINVMVIGVRPDGKINLSRKALLEKEAAERAATAQAPA
EEHHHCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCC
DGRSHQPRAPQRPSGTAQPERRPGPPTPRRPEQRGPSRPPRPQAQRSTPPPGQYRIGDRL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
KELLGEDEPN
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA