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The map label for this gene is folC [H]

Identifier: 221632549

GI number: 221632549

Start: 521015

End: 522469

Strand: Reverse

Name: folC [H]

Synonym: trd_0524

Alternate gene names: 221632549

Gene position: 522469-521015 (Counterclockwise)

Preceding gene: 221632556

Following gene: 221632548

Centisome position: 26.08

GC content: 61.72

Gene sequence:

>1455_bases
ATGAGCGAGGCGTCCGTGCCCGACGCACGTTATCGATGGGCGACCGGGTACCTCCGGGCACTCATTCAAGCTCCACCCGG
TCCCCCTCCTGGGACCCCGCCAGAGATCGTCCGCCTGCGGGCCCAACGCCGATTGGAACGACTGCGTGACTTCCTCGCCT
ACCTCGGCAACCCGCATCGGGTCTATCCCGTCATCCACATCACTGGCACCTCGGGCAAAGGTTCGACCGCTGCCTTCATC
GCCAATATCCTGCATGTTGCTGGATACCGCACAGGCCTGCACACTTCTCCATACTTGCAGGTAGAAACGGAGAAGCTCCA
GATCGCAGGACAACTCATCGCACCGGAGCGATTCGCTGAGGAGGTCGCCATACTGGACCAAGCGGTTCGCTCGTGGATCG
CCCGAGGTGGTCAACCGCTCACCTACGGCGAATTTTGGACTGCGCTCGTCTTTCAGACTTTGCGATCGGAGCGGGTGGAT
GTCGCCGTGATCGAAGTCAGCGTCGGAGGACGCTTCGACCTCACCAATGTCGTCCTGCCGGAAGTCGCGGTGATTACCAC
CGTGGGGCTCGACCACGTCCGCACGCTCGGCCCGACAATCCGTGACATCGCCTGGCACAAGGCAGGTATCATCAAGCCGG
GTCGTCCCGCCATCACCGGAGTCACTGATCCCGAGTTGCTCGCCATCATCGCCGCGGAAGCCGACGCTGTCGGCGCATCC
CTCACGGTCGTGCGCGAAGGCAGCGATTATTGGGTCACCTCGACCGGTCTCGGCACAACACTCACTGACCTCCGCACTGG
GCACACCTTTCCGATCGGCTTATTAGGATCGTATCAAGCTCGCAATGCGGCACTGGCCATCGCTGCCATCCGCACGTTCG
CCGATCGTATCGGAGGACGGATCGATGACGAAACGATCGCACGCGGCTTGGTGACCACCCGCTTCCCGGGGCGCTTCGAG
ATCATCCAGGAGACACCCCTCGTCATTCTCGATGGTGCGCATAACCCGCAAAAAATGGCGGCTTTGCTCGAAGCGCTGGA
CGCATTGTCCGTGCAGGGGCGTCGACTAGCTCTCTTCGGCGTCCTGGAAGGACACGATGCGCTGACGATGGCCACGATGC
TGGCAAAAAGTGTCGACATGGTGGTGCTCACCACCCCACGAGCATTCTTACGAACTGCTGCCGATCCGGTCGAACTCGCA
GCGGCCTGTCAGGCGCAAGGGACACACGCGGTCACCGTCGATGATCCAGCTGAGGCGCTCGCCCTGGTCCTCGAAATGGC
ACAGCCGAACGATCTCATCGTGGTCACTGGCTCGCTCTACCTGGTCGGGCACGTCCGTGAGCACTGGTATCCGAGTTCGC
TCATCGTCACGCAGCGAACCTGCTGGCCGATTCGCCGATCGATCGCGATTCAGGGAGCCGAAAGCGCTTGTCGACAGCAA
CACGGTTCGGTATAG

Upstream 100 bases:

>100_bases
GGAAAGTCTCTTCCCTCGCGAAACGTCATACTTCAGGTTGCACCGGCTGCGAGAATCGTCGGACGAGCCACATCCAGTCT
AACCGGAGGAGTCGGATCCG

Downstream 100 bases:

>100_bases
AATGACCATGACGCCGACAATCCTGGCGATCGAGACAGTATGCGCGAGCGGCATGGGAGGGATGGAACCGTTGGAGCGCC
CGATCGACGTCGAACTTCTC

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MSEASVPDARYRWATGYLRALIQAPPGPPPGTPPEIVRLRAQRRLERLRDFLAYLGNPHRVYPVIHITGTSGKGSTAAFI
ANILHVAGYRTGLHTSPYLQVETEKLQIAGQLIAPERFAEEVAILDQAVRSWIARGGQPLTYGEFWTALVFQTLRSERVD
VAVIEVSVGGRFDLTNVVLPEVAVITTVGLDHVRTLGPTIRDIAWHKAGIIKPGRPAITGVTDPELLAIIAAEADAVGAS
LTVVREGSDYWVTSTGLGTTLTDLRTGHTFPIGLLGSYQARNAALAIAAIRTFADRIGGRIDDETIARGLVTTRFPGRFE
IIQETPLVILDGAHNPQKMAALLEALDALSVQGRRLALFGVLEGHDALTMATMLAKSVDMVVLTTPRAFLRTAADPVELA
AACQAQGTHAVTVDDPAEALALVLEMAQPNDLIVVTGSLYLVGHVREHWYPSSLIVTQRTCWPIRRSIAIQGAESACRQQ
HGSV

Sequences:

>Translated_484_residues
MSEASVPDARYRWATGYLRALIQAPPGPPPGTPPEIVRLRAQRRLERLRDFLAYLGNPHRVYPVIHITGTSGKGSTAAFI
ANILHVAGYRTGLHTSPYLQVETEKLQIAGQLIAPERFAEEVAILDQAVRSWIARGGQPLTYGEFWTALVFQTLRSERVD
VAVIEVSVGGRFDLTNVVLPEVAVITTVGLDHVRTLGPTIRDIAWHKAGIIKPGRPAITGVTDPELLAIIAAEADAVGAS
LTVVREGSDYWVTSTGLGTTLTDLRTGHTFPIGLLGSYQARNAALAIAAIRTFADRIGGRIDDETIARGLVTTRFPGRFE
IIQETPLVILDGAHNPQKMAALLEALDALSVQGRRLALFGVLEGHDALTMATMLAKSVDMVVLTTPRAFLRTAADPVELA
AACQAQGTHAVTVDDPAEALALVLEMAQPNDLIVVTGSLYLVGHVREHWYPSSLIVTQRTCWPIRRSIAIQGAESACRQQ
HGSV
>Mature_483_residues
SEASVPDARYRWATGYLRALIQAPPGPPPGTPPEIVRLRAQRRLERLRDFLAYLGNPHRVYPVIHITGTSGKGSTAAFIA
NILHVAGYRTGLHTSPYLQVETEKLQIAGQLIAPERFAEEVAILDQAVRSWIARGGQPLTYGEFWTALVFQTLRSERVDV
AVIEVSVGGRFDLTNVVLPEVAVITTVGLDHVRTLGPTIRDIAWHKAGIIKPGRPAITGVTDPELLAIIAAEADAVGASL
TVVREGSDYWVTSTGLGTTLTDLRTGHTFPIGLLGSYQARNAALAIAAIRTFADRIGGRIDDETIARGLVTTRFPGRFEI
IQETPLVILDGAHNPQKMAALLEALDALSVQGRRLALFGVLEGHDALTMATMLAKSVDMVVLTTPRAFLRTAADPVELAA
ACQAQGTHAVTVDDPAEALALVLEMAQPNDLIVVTGSLYLVGHVREHWYPSSLIVTQRTCWPIRRSIAIQGAESACRQQH
GSV

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=319, Percent_Identity=32.6018808777429, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI66932984, Length=319, Percent_Identity=32.6018808777429, Blast_Score=138, Evalue=1e-32,
Organism=Escherichia coli, GI1788654, Length=407, Percent_Identity=32.4324324324324, Blast_Score=162, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17553150, Length=466, Percent_Identity=27.4678111587983, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI71984923, Length=466, Percent_Identity=27.4678111587983, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17553148, Length=466, Percent_Identity=27.4678111587983, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6324815, Length=449, Percent_Identity=29.8440979955457, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6323760, Length=427, Percent_Identity=26.9320843091335, Blast_Score=129, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24641571, Length=285, Percent_Identity=34.0350877192982, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24581568, Length=329, Percent_Identity=26.7477203647416, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 52239; Mature: 52108

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEASVPDARYRWATGYLRALIQAPPGPPPGTPPEIVRLRAQRRLERLRDFLAYLGNPHR
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
VYPVIHITGTSGKGSTAAFIANILHVAGYRTGLHTSPYLQVETEKLQIAGQLIAPERFAE
EEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEHHEEEEEHHCCCHHHHHH
EVAILDQAVRSWIARGGQPLTYGEFWTALVFQTLRSERVDVAVIEVSVGGRFDLTNVVLP
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHC
EVAVITTVGLDHVRTLGPTIRDIAWHKAGIIKPGRPAITGVTDPELLAIIAAEADAVGAS
CCHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHEEEEEECCCCCCCE
LTVVREGSDYWVTSTGLGTTLTDLRTGHTFPIGLLGSYQARNAALAIAAIRTFADRIGGR
EEEEEECCCEEEEECCCCCCHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCC
IDDETIARGLVTTRFPGRFEIIQETPLVILDGAHNPQKMAALLEALDALSVQGRRLALFG
CCHHHHHHCCCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEE
VLEGHDALTMATMLAKSVDMVVLTTPRAFLRTAADPVELAAACQAQGTHAVTVDDPAEAL
EECCCCHHHHHHHHHHCCCEEEEECCHHHHHCCCCHHHHHHHHHCCCCEEEEECCHHHHH
ALVLEMAQPNDLIVVTGSLYLVGHVREHWYPSSLIVTQRTCWPIRRSIAIQGAESACRQQ
HHHHHHCCCCCEEEEECCEEEEEEHHHCCCCCCEEEEECCCHHHHHHHEECCHHHHHHHH
HGSV
CCCC
>Mature Secondary Structure 
SEASVPDARYRWATGYLRALIQAPPGPPPGTPPEIVRLRAQRRLERLRDFLAYLGNPHR
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
VYPVIHITGTSGKGSTAAFIANILHVAGYRTGLHTSPYLQVETEKLQIAGQLIAPERFAE
EEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEHHEEEEEHHCCCHHHHHH
EVAILDQAVRSWIARGGQPLTYGEFWTALVFQTLRSERVDVAVIEVSVGGRFDLTNVVLP
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHC
EVAVITTVGLDHVRTLGPTIRDIAWHKAGIIKPGRPAITGVTDPELLAIIAAEADAVGAS
CCHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHEEEEEECCCCCCCE
LTVVREGSDYWVTSTGLGTTLTDLRTGHTFPIGLLGSYQARNAALAIAAIRTFADRIGGR
EEEEEECCCEEEEECCCCCCHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCC
IDDETIARGLVTTRFPGRFEIIQETPLVILDGAHNPQKMAALLEALDALSVQGRRLALFG
CCHHHHHHCCCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEE
VLEGHDALTMATMLAKSVDMVVLTTPRAFLRTAADPVELAAACQAQGTHAVTVDDPAEAL
EECCCCHHHHHHHHHHCCCEEEEECCHHHHHCCCCHHHHHHHHHCCCCEEEEECCHHHHH
ALVLEMAQPNDLIVVTGSLYLVGHVREHWYPSSLIVTQRTCWPIRRSIAIQGAESACRQQ
HHHHHHCCCCCEEEEECCEEEEEEHHHCCCCCCEEEEECCCHHHHHHHEECCHHHHHHHH
HGSV
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]