Click here to switch to the map view.

The map label for this gene is icl [H]

Identifier: 221632522

GI number: 221632522

Start: 492595

End: 493773

Strand: Reverse

Name: icl [H]

Synonym: trd_0496

Alternate gene names: 221632522

Gene position: 493773-492595 (Counterclockwise)

Preceding gene: 221632524

Following gene: 221632521

Centisome position: 24.65

GC content: 61.83

Gene sequence:

>1179_bases
GTGAAGGGAGAATTCGTCATGTGGTCGCTGCCGCAAGCCGTGACTGATCCGAACGAACTCCGCGAAACCCCGCTCCTTCC
CCCTGGGTATCGCGTTCCAGCCTCGCGCTGGATTCACCCGAGCACGCGACTGCGTCAGCTCCTCGAGACCCTCCCCTACG
TGTTCGCCCCTGGTGTCTATGACCCCCACGGCGCACAACTCGTCATGTATTACGGCTTTCCGGCTGTCTACTTCAGCGGC
TATTCCTTCGCGATCGGCCATCTGGGCACGACGGATATGGATCTGTACTCCAGCGTGGAGATCGCCGATGCTGCACGCCG
CATCGTCGCCGGGCTACGCAAGTTCCAGCTCACGATGGCTGTCGGCGATCCGGAGAAGGGCATACCCCCGAAGCATCTCC
ATATCCCACCGGTCGTCGCCGACATGGATACCGGGTACGGAAACCTCTTCAACGTCCAGCGCACCACCGAGCTCTATGTG
ACCGCGGGCGTCGCTGGCGCGCATATCGAGGACCAAGTGATGCCGAAGCGCTGCGGCCACATCGCGGGCAAGGCGCTCAT
CCCAGCACAAGAAATGGTCGGCAAACTCAAGATGATGCGGGCAGTGGCCAACGATCTCGGGAATCCCGATTTCGTCATCA
TCGCACGCACCGACGGCGTTTCGGCCGTCGACGCGCCGGAAGCGAAGCGCGGCCTGCCGTTGGCGATCGAGCGTGCCCTG
CGCTACCTCGACAGTGGCGTCCCCGATCTGGTGTGGTGCGAGTTTCCGACCTCAGAGCGAGGGCCACTCGAAACGTTCGT
CGAGGAAGTGCGCAAGCGCTTCCCGGATGCTCGGTTCGCCTTCAACTGGTCGAGTTCATTCAAGTGGTTCACTGACCCGA
ACCCGATCAGCTTCCGCGAACTCGGTGAGATGGGCGTGCGGTTCATCTTCATCACGCTGGCAGCACAACACGCGATGGGG
CTCGGTTTCAGCGAACTGCTCCAGCATCTGGCAGCACGCCAGGAGCGAGCGTACATCGACCTGCAAAAGCGCGAGTGGGC
CGAAGGCACCGACTTCCCCACCCGAAGCCATCACTTCTTCTCCGGTGTTCCCTACCATCACCTCCTGGGGCAGGTGTACG
ACGCACCTCGACTGGGAACCCAATTCGAAGAGGAGTTGCCGGAAGAAGCAGTGGTCTGA

Upstream 100 bases:

>100_bases
GCACAGTGAGACCCCGCCTACGCGGGGGCCACCAACTCATCGCCCAGCGTGCATCGCTCGCGACACGCTCCGAAACATCC
GCTCGTCAGTCAGGAGACCA

Downstream 100 bases:

>100_bases
GTCGTTCTCCTGCCGGGATGCAGGGTTTCCGAACTCCGCATCCCGGCTTTTCTTCGTTACACTAGGGGTGACGCTGTGGT
GCCATCACCGGGTGAGGGAG

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MKGEFVMWSLPQAVTDPNELRETPLLPPGYRVPASRWIHPSTRLRQLLETLPYVFAPGVYDPHGAQLVMYYGFPAVYFSG
YSFAIGHLGTTDMDLYSSVEIADAARRIVAGLRKFQLTMAVGDPEKGIPPKHLHIPPVVADMDTGYGNLFNVQRTTELYV
TAGVAGAHIEDQVMPKRCGHIAGKALIPAQEMVGKLKMMRAVANDLGNPDFVIIARTDGVSAVDAPEAKRGLPLAIERAL
RYLDSGVPDLVWCEFPTSERGPLETFVEEVRKRFPDARFAFNWSSSFKWFTDPNPISFRELGEMGVRFIFITLAAQHAMG
LGFSELLQHLAARQERAYIDLQKREWAEGTDFPTRSHHFFSGVPYHHLLGQVYDAPRLGTQFEEELPEEAVV

Sequences:

>Translated_392_residues
MKGEFVMWSLPQAVTDPNELRETPLLPPGYRVPASRWIHPSTRLRQLLETLPYVFAPGVYDPHGAQLVMYYGFPAVYFSG
YSFAIGHLGTTDMDLYSSVEIADAARRIVAGLRKFQLTMAVGDPEKGIPPKHLHIPPVVADMDTGYGNLFNVQRTTELYV
TAGVAGAHIEDQVMPKRCGHIAGKALIPAQEMVGKLKMMRAVANDLGNPDFVIIARTDGVSAVDAPEAKRGLPLAIERAL
RYLDSGVPDLVWCEFPTSERGPLETFVEEVRKRFPDARFAFNWSSSFKWFTDPNPISFRELGEMGVRFIFITLAAQHAMG
LGFSELLQHLAARQERAYIDLQKREWAEGTDFPTRSHHFFSGVPYHHLLGQVYDAPRLGTQFEEELPEEAVV
>Mature_392_residues
MKGEFVMWSLPQAVTDPNELRETPLLPPGYRVPASRWIHPSTRLRQLLETLPYVFAPGVYDPHGAQLVMYYGFPAVYFSG
YSFAIGHLGTTDMDLYSSVEIADAARRIVAGLRKFQLTMAVGDPEKGIPPKHLHIPPVVADMDTGYGNLFNVQRTTELYV
TAGVAGAHIEDQVMPKRCGHIAGKALIPAQEMVGKLKMMRAVANDLGNPDFVIIARTDGVSAVDAPEAKRGLPLAIERAL
RYLDSGVPDLVWCEFPTSERGPLETFVEEVRKRFPDARFAFNWSSSFKWFTDPNPISFRELGEMGVRFIFITLAAQHAMG
LGFSELLQHLAARQERAYIDLQKREWAEGTDFPTRSHHFFSGVPYHHLLGQVYDAPRLGTQFEEELPEEAVV

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=327, Percent_Identity=30.2752293577982, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1786525, Length=190, Percent_Identity=35.2631578947368, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71982926, Length=367, Percent_Identity=30.7901907356948, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17561814, Length=367, Percent_Identity=30.7901907356948, Blast_Score=141, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6320908, Length=100, Percent_Identity=42, Blast_Score=83, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6325263, Length=279, Percent_Identity=26.8817204301075, Blast_Score=76, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 43889; Mature: 43889

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGEFVMWSLPQAVTDPNELRETPLLPPGYRVPASRWIHPSTRLRQLLETLPYVFAPGVY
CCCCEEEEECCHHHCCHHHHHCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCC
DPHGAQLVMYYGFPAVYFSGYSFAIGHLGTTDMDLYSSVEIADAARRIVAGLRKFQLTMA
CCCCCEEEEEECCHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEE
VGDPEKGIPPKHLHIPPVVADMDTGYGNLFNVQRTTELYVTAGVAGAHIEDQVMPKRCGH
ECCCCCCCCCCCEECCCEEEECCCCCCCEEEEEECEEEEEEECCCCCCCHHHHHHHHHHH
IAGKALIPAQEMVGKLKMMRAVANDLGNPDFVIIARTDGVSAVDAPEAKRGLPLAIERAL
HCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHCCCCHHHHHHH
RYLDSGVPDLVWCEFPTSERGPLETFVEEVRKRFPDARFAFNWSSSFKWFTDPNPISFRE
HHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEECCCCCCCHHH
LGEMGVRFIFITLAAQHAMGLGFSELLQHLAARQERAYIDLQKREWAEGTDFPTRSHHFF
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEHHHCCCCCCCCCCCCCCCC
SGVPYHHLLGQVYDAPRLGTQFEEELPEEAVV
CCCCHHHHHHHHHCCCCCCCHHHHHCCHHCCC
>Mature Secondary Structure
MKGEFVMWSLPQAVTDPNELRETPLLPPGYRVPASRWIHPSTRLRQLLETLPYVFAPGVY
CCCCEEEEECCHHHCCHHHHHCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCC
DPHGAQLVMYYGFPAVYFSGYSFAIGHLGTTDMDLYSSVEIADAARRIVAGLRKFQLTMA
CCCCCEEEEEECCHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEE
VGDPEKGIPPKHLHIPPVVADMDTGYGNLFNVQRTTELYVTAGVAGAHIEDQVMPKRCGH
ECCCCCCCCCCCEECCCEEEECCCCCCCEEEEEECEEEEEEECCCCCCCHHHHHHHHHHH
IAGKALIPAQEMVGKLKMMRAVANDLGNPDFVIIARTDGVSAVDAPEAKRGLPLAIERAL
HCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHCCCCHHHHHHH
RYLDSGVPDLVWCEFPTSERGPLETFVEEVRKRFPDARFAFNWSSSFKWFTDPNPISFRE
HHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEECCCCCCCHHH
LGEMGVRFIFITLAAQHAMGLGFSELLQHLAARQERAYIDLQKREWAEGTDFPTRSHHFF
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEHHHCCCCCCCCCCCCCCCC
SGVPYHHLLGQVYDAPRLGTQFEEELPEEAVV
CCCCHHHHHHHHHCCCCCCCHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045408 [H]