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The map label for this gene is dnaG [H]

Identifier: 221632448

GI number: 221632448

Start: 417929

End: 419779

Strand: Reverse

Name: dnaG [H]

Synonym: trd_0421

Alternate gene names: 221632448

Gene position: 419779-417929 (Counterclockwise)

Preceding gene: 221632449

Following gene: 221632446

Centisome position: 20.96

GC content: 64.07

Gene sequence:

>1851_bases
ATGGGACGCCAGGACGTCGAACGAGTCCGAGAAGCCATCGATATCGTCGACCTGATCGGCGGGTACATCCAGCTGCGCAA
AGCCGGGAAAAACTTCAAGGCCCTTTGTCCTTTTCACCAGGAGAAAACCCCCTCTTTTTATGTTTTTCCCGAAACGCAAT
CGTTCTATTGCTTCGGCTGTGGGGCGAGCGGTGACGCGATCAGTTTCCTGATGCGCATCGAGCAACTCGACTTCCGCGAT
GCCCTCGAGCGACTGGCCGAGCGGGCAGGGATCACGCTCTCGCCACCCCGACCGGAGGAACGTGCCGAAGACGAGCAGCG
TCGCCGACTGGCCGAACTGAATCGCTTGGCCGCGGCATGGTTCGCGCACGTGCTGTGGGGGACATCGTTCGGTGAGGCAG
CGCGTTCCTACCTCGAACGGCGCGGTATCGATCGCCCTACCGCCGAGCGCTTCGGGCTCGGCTACGCACCCGATGCGCCC
AGTGCACTCGCCCGTTACCTCTCTCAGCACGGTGCCGGACCGGAGGAACTCGTCACTGCCGGCCTCGTCGTGCGCCGAGA
CGATGGCTCTCTCGTAGACCGCTTCCGCAACCGACTCATCTTCCCCATCCGAGACCGACACGGTACCATCCTCGGCTTCG
GAGGACGCACGTTGGGCGAGGCACAACCCAAATACCTCAACTCGCCACAAACCCCACTGTTCGACAAAGGAAGTGTGCTG
TACGCGATCGATCTCGCCGAGGAGTCGATTCGCCAGCAGCGTACCGCAATCGTGGTCGAAGGCTATCTGGATGCGATCAC
AGCGCATCAGTTCGGCTACTCGAACACCGTCGCCTCGCTCGGCACCGCCTTGACCGAGCGGCAGGGGAGACACCTCCGCC
GTCTCGCCGAGCGTATCCTGCTCGCGCTCGATGCCGATGTCGCCGGACGCCAGGCGACCTTGCGTGGATTGGAGCTGCTT
CGCCACGCTCTCGCCGAGGGAGAGCGTGCCGTCGTCGATCCCCGCCAACTCATTCGCTTCGAGCGCACCCTGGATGTCGA
ACTCGCCGTCGTGGTGCTCCCGGAGGGCCTCGACCCCGACGACCTCATCCGGCAAGATCGCACCCGTTGGGACGAAGCGC
TCGCCCATGCAGTGCCGCTCGTCGATTACTACTTGGACGTGCTCCTCGGTGAGCAGCCTCCCGCGAATCCTCGAGCGGCC
ACCGCGTTGCTCCAACGCATCGCCCCGCTCCTCCTCGAACTCGGCGACCTGGTTCAGGTCGATCTCTACACGCGTTCGGT
TGCGGCCCGGCTGCGTCTGCCTGAAGAGACGGTTCGACGCCTTCTCGCCTCGCTCCGACGCCAACCGACTCGAGAACCCT
CACCCAAGACGACAGCAGCGGCAATCCTTCGGCCGCTCTCCACCGAGCAGCAATTCGTCGGCCTTCTCCTACGCTATCCC
GAGGCAGCAGCCCATCTGGTCGGCGAAATCGACCTGGAGTCGATCACCGATGCCCGTTATCGCGAACTCGTCAGCCTGCT
CCTCGCCGGTCAACCGCTCGACGACGCAGCGCTAGCCGAGGATCTCCGGGACGACATCGGCCAGCTGCGCCAGCGATTCG
CGACACAACCCGACCTCCCCGTGCCGCTCGCTCGCCAGGCTATCCGGGAAGCCTTTCGCAGGCTCCGTCGCGAACGTCAC
GACGAGCGGTTGCGTTCGCTCTTGAGCGAGGTTCGCGATGCCGAAGAGCGGGGAGACCGGGACGAACTCCGGCGTGCGTT
GGAGTTGATCGAAGCGCTCAAGCAGCGCTTCCCCGAGTTCTATCCGGAACGCAGCCCCTATTTCCGCGATACGCGGGATC
CGGTCAACTGA

Upstream 100 bases:

>100_bases
ATCGCTGCTGATTACGTCGCGAGCATGACGGATCGCTACGCGCTCGCTTGCTTCGAAGCGATCTGGCTACCGCGCTTTTG
GACATTCCTCGGATAGATCG

Downstream 100 bases:

>100_bases
TGGCGAGAGAACGGCGTAGCGCCTCGACGAGTGCCTCGTCTTGCTCTGGCAAGACTGTCCGGGCATCCAGGAGCAGGCGG
TCGGCTTCGACTCGCCCCAC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 616; Mature: 615

Protein sequence:

>616_residues
MGRQDVERVREAIDIVDLIGGYIQLRKAGKNFKALCPFHQEKTPSFYVFPETQSFYCFGCGASGDAISFLMRIEQLDFRD
ALERLAERAGITLSPPRPEERAEDEQRRRLAELNRLAAAWFAHVLWGTSFGEAARSYLERRGIDRPTAERFGLGYAPDAP
SALARYLSQHGAGPEELVTAGLVVRRDDGSLVDRFRNRLIFPIRDRHGTILGFGGRTLGEAQPKYLNSPQTPLFDKGSVL
YAIDLAEESIRQQRTAIVVEGYLDAITAHQFGYSNTVASLGTALTERQGRHLRRLAERILLALDADVAGRQATLRGLELL
RHALAEGERAVVDPRQLIRFERTLDVELAVVVLPEGLDPDDLIRQDRTRWDEALAHAVPLVDYYLDVLLGEQPPANPRAA
TALLQRIAPLLLELGDLVQVDLYTRSVAARLRLPEETVRRLLASLRRQPTREPSPKTTAAAILRPLSTEQQFVGLLLRYP
EAAAHLVGEIDLESITDARYRELVSLLLAGQPLDDAALAEDLRDDIGQLRQRFATQPDLPVPLARQAIREAFRRLRRERH
DERLRSLLSEVRDAEERGDRDELRRALELIEALKQRFPEFYPERSPYFRDTRDPVN

Sequences:

>Translated_616_residues
MGRQDVERVREAIDIVDLIGGYIQLRKAGKNFKALCPFHQEKTPSFYVFPETQSFYCFGCGASGDAISFLMRIEQLDFRD
ALERLAERAGITLSPPRPEERAEDEQRRRLAELNRLAAAWFAHVLWGTSFGEAARSYLERRGIDRPTAERFGLGYAPDAP
SALARYLSQHGAGPEELVTAGLVVRRDDGSLVDRFRNRLIFPIRDRHGTILGFGGRTLGEAQPKYLNSPQTPLFDKGSVL
YAIDLAEESIRQQRTAIVVEGYLDAITAHQFGYSNTVASLGTALTERQGRHLRRLAERILLALDADVAGRQATLRGLELL
RHALAEGERAVVDPRQLIRFERTLDVELAVVVLPEGLDPDDLIRQDRTRWDEALAHAVPLVDYYLDVLLGEQPPANPRAA
TALLQRIAPLLLELGDLVQVDLYTRSVAARLRLPEETVRRLLASLRRQPTREPSPKTTAAAILRPLSTEQQFVGLLLRYP
EAAAHLVGEIDLESITDARYRELVSLLLAGQPLDDAALAEDLRDDIGQLRQRFATQPDLPVPLARQAIREAFRRLRRERH
DERLRSLLSEVRDAEERGDRDELRRALELIEALKQRFPEFYPERSPYFRDTRDPVN
>Mature_615_residues
GRQDVERVREAIDIVDLIGGYIQLRKAGKNFKALCPFHQEKTPSFYVFPETQSFYCFGCGASGDAISFLMRIEQLDFRDA
LERLAERAGITLSPPRPEERAEDEQRRRLAELNRLAAAWFAHVLWGTSFGEAARSYLERRGIDRPTAERFGLGYAPDAPS
ALARYLSQHGAGPEELVTAGLVVRRDDGSLVDRFRNRLIFPIRDRHGTILGFGGRTLGEAQPKYLNSPQTPLFDKGSVLY
AIDLAEESIRQQRTAIVVEGYLDAITAHQFGYSNTVASLGTALTERQGRHLRRLAERILLALDADVAGRQATLRGLELLR
HALAEGERAVVDPRQLIRFERTLDVELAVVVLPEGLDPDDLIRQDRTRWDEALAHAVPLVDYYLDVLLGEQPPANPRAAT
ALLQRIAPLLLELGDLVQVDLYTRSVAARLRLPEETVRRLLASLRRQPTREPSPKTTAAAILRPLSTEQQFVGLLLRYPE
AAAHLVGEIDLESITDARYRELVSLLLAGQPLDDAALAEDLRDDIGQLRQRFATQPDLPVPLARQAIREAFRRLRRERHD
ERLRSLLSEVRDAEERGDRDELRRALELIEALKQRFPEFYPERSPYFRDTRDPVN

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=531, Percent_Identity=34.4632768361582, Blast_Score=264, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 69696; Mature: 69565

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRQDVERVREAIDIVDLIGGYIQLRKAGKNFKALCPFHQEKTPSFYVFPETQSFYCFGC
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCEEEEECCCCEEEEEC
GASGDAISFLMRIEQLDFRDALERLAERAGITLSPPRPEERAEDEQRRRLAELNRLAAAW
CCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHH
FAHVLWGTSFGEAARSYLERRGIDRPTAERFGLGYAPDAPSALARYLSQHGAGPEELVTA
HHHHHHCCCHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHH
GLVVRRDDGSLVDRFRNRLIFPIRDRHGTILGFGGRTLGEAQPKYLNSPQTPLFDKGSVL
HHEEECCCCHHHHHHHHCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEE
YAIDLAEESIRQQRTAIVVEGYLDAITAHQFGYSNTVASLGTALTERQGRHLRRLAERIL
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALDADVAGRQATLRGLELLRHALAEGERAVVDPRQLIRFERTLDVELAVVVLPEGLDPD
HHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCEEEEEEEECCCCCHH
DLIRQDRTRWDEALAHAVPLVDYYLDVLLGEQPPANPRAATALLQRIAPLLLELGDLVQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DLYTRSVAARLRLPEETVRRLLASLRRQPTREPSPKTTAAAILRPLSTEQQFVGLLLRYP
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCC
EAAAHLVGEIDLESITDARYRELVSLLLAGQPLDDAALAEDLRDDIGQLRQRFATQPDLP
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
VPLARQAIREAFRRLRRERHDERLRSLLSEVRDAEERGDRDELRRALELIEALKQRFPEF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHH
YPERSPYFRDTRDPVN
CCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GRQDVERVREAIDIVDLIGGYIQLRKAGKNFKALCPFHQEKTPSFYVFPETQSFYCFGC
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCEEEEECCCCEEEEEC
GASGDAISFLMRIEQLDFRDALERLAERAGITLSPPRPEERAEDEQRRRLAELNRLAAAW
CCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHH
FAHVLWGTSFGEAARSYLERRGIDRPTAERFGLGYAPDAPSALARYLSQHGAGPEELVTA
HHHHHHCCCHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHH
GLVVRRDDGSLVDRFRNRLIFPIRDRHGTILGFGGRTLGEAQPKYLNSPQTPLFDKGSVL
HHEEECCCCHHHHHHHHCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEE
YAIDLAEESIRQQRTAIVVEGYLDAITAHQFGYSNTVASLGTALTERQGRHLRRLAERIL
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALDADVAGRQATLRGLELLRHALAEGERAVVDPRQLIRFERTLDVELAVVVLPEGLDPD
HHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCEEEEEEEECCCCCHH
DLIRQDRTRWDEALAHAVPLVDYYLDVLLGEQPPANPRAATALLQRIAPLLLELGDLVQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DLYTRSVAARLRLPEETVRRLLASLRRQPTREPSPKTTAAAILRPLSTEQQFVGLLLRYP
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCC
EAAAHLVGEIDLESITDARYRELVSLLLAGQPLDDAALAEDLRDDIGQLRQRFATQPDLP
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
VPLARQAIREAFRRLRRERHDERLRSLLSEVRDAEERGDRDELRRALELIEALKQRFPEF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHH
YPERSPYFRDTRDPVN
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961408; 11466286 [H]