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The map label for this gene is mdeA [H]

Identifier: 221632420

GI number: 221632420

Start: 392910

End: 394118

Strand: Reverse

Name: mdeA [H]

Synonym: trd_0392

Alternate gene names: 221632420

Gene position: 394118-392910 (Counterclockwise)

Preceding gene: 221632425

Following gene: 221632419

Centisome position: 19.68

GC content: 62.61

Gene sequence:

>1209_bases
ATGGACGATCAGCGCCGACTCCACTTCCGATTCGCAACTCGTGCCGTACACGCGGGCGAACGTCCGAATCCTCGCGACTT
CATCCCGACCGCGACCCCGATCTATCCGACCTCGACATTCCTGTACCAGAACATCGAGCAGATGGACGCGGCGCTCGGCG
GTGCTGTGGGGGTCTTCGTGTATGGACGCTATGGCAACCCCACGACGGAAGCACTCGAGACAGCAGTCGCCTCTCTCGAA
GAGCAGGAAGCAGCGCTCGCCTTTGCCTCCGGAATGGCCGCCCTGCACGCCACGATCCTCTCCTGCGTCGAGGCAGGGGA
TCGTATCGTCGCCTCGCGCGATCTGTATGGTCAGACTATCACTCTCCTCCGCACGATCTTCGGATCGCTCGGGGTGCGAG
TCGAGTTCGTCGATATCCTCGATCTCGCTCAGGTGGAAGCTGCATTGCGCCGCGGTGGGGTGCGGTTGCTCCTGTGTGAG
ACCATCTCCAATCCGCTGCTCCGCGTCACCGATCTTCCCAGCCTCGCTGAAATCGCCCACCGTTATGGCGCTCGGCTGGC
GGTCGACAACACCTTCGCCACACCCTATCTCGTGCGTCCCGGGACGCTCGGGGCCGATTACGTGATCCACAGCGCGACGA
AGTATCTTAGTGGACACGGCGATGTGACCGCGGGGATCGTCGCGACAACAGCCGATCGCCGCTGGGAACTCAACGAGATC
AACAAGACGATCGGCAGTATCCTCGGTCCGTTCGAGGCCTTCCTGGTTCTCCGCGGCCTCAAGACGCTGCCGCTCCGCAT
GCACCGTCAATGCGAGAACGCCAGTCGGATCGCTTGCTGGCTCGCTGAACATCCGCTGATCGAGCGCGTCCATTATCCGG
GCCTGCCCACTCATCCCAGTCACGAGACCGCTGCGCGCCTCCTCCCTCCGGGACTCTACGGCGCCATCGTGTCGTTCGAA
ATTCGCCACGGGACACGCGAACGCGTGACGGCGTTCCTCGAACGCTTGCGGCTCATTCTGCCAGCAACGACGCTGGGTGA
CGTCTATTCCGAGATCCTCTATCCAGTCATGGCCTCGCATCGCGGCCTTTCACCAGAAGAGCGCCAGGCAATGGGTATCA
GCGACGGGCTCGTCCGGCTCTCGGTCGGTATCGAGGATCCCGAGGACATCATCGCCGATCTGGAACAGGCACTGGCAAGC
CTTGCCTGA

Upstream 100 bases:

>100_bases
CGCAAGCCGCCGAGCGGGCAAGCGTCGCACGAGGCTTTATACTTCCGCGCGAAACCTGACGGAGAGCGGGCTGGCGAGCA
GCGAAGAGGGCAGGGAGACG

Downstream 100 bases:

>100_bases
CTCATGCGACCTGGTGACCGCGGCTGCCGCAAGAGACAGACAGCTTGCTGGCTCGCCACCACGTGTCGGTGCGCTCCTCG
GTGGGGTGACCAGTTCGTTC

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MDDQRRLHFRFATRAVHAGERPNPRDFIPTATPIYPTSTFLYQNIEQMDAALGGAVGVFVYGRYGNPTTEALETAVASLE
EQEAALAFASGMAALHATILSCVEAGDRIVASRDLYGQTITLLRTIFGSLGVRVEFVDILDLAQVEAALRRGGVRLLLCE
TISNPLLRVTDLPSLAEIAHRYGARLAVDNTFATPYLVRPGTLGADYVIHSATKYLSGHGDVTAGIVATTADRRWELNEI
NKTIGSILGPFEAFLVLRGLKTLPLRMHRQCENASRIACWLAEHPLIERVHYPGLPTHPSHETAARLLPPGLYGAIVSFE
IRHGTRERVTAFLERLRLILPATTLGDVYSEILYPVMASHRGLSPEERQAMGISDGLVRLSVGIEDPEDIIADLEQALAS
LA

Sequences:

>Translated_402_residues
MDDQRRLHFRFATRAVHAGERPNPRDFIPTATPIYPTSTFLYQNIEQMDAALGGAVGVFVYGRYGNPTTEALETAVASLE
EQEAALAFASGMAALHATILSCVEAGDRIVASRDLYGQTITLLRTIFGSLGVRVEFVDILDLAQVEAALRRGGVRLLLCE
TISNPLLRVTDLPSLAEIAHRYGARLAVDNTFATPYLVRPGTLGADYVIHSATKYLSGHGDVTAGIVATTADRRWELNEI
NKTIGSILGPFEAFLVLRGLKTLPLRMHRQCENASRIACWLAEHPLIERVHYPGLPTHPSHETAARLLPPGLYGAIVSFE
IRHGTRERVTAFLERLRLILPATTLGDVYSEILYPVMASHRGLSPEERQAMGISDGLVRLSVGIEDPEDIIADLEQALAS
LA
>Mature_402_residues
MDDQRRLHFRFATRAVHAGERPNPRDFIPTATPIYPTSTFLYQNIEQMDAALGGAVGVFVYGRYGNPTTEALETAVASLE
EQEAALAFASGMAALHATILSCVEAGDRIVASRDLYGQTITLLRTIFGSLGVRVEFVDILDLAQVEAALRRGGVRLLLCE
TISNPLLRVTDLPSLAEIAHRYGARLAVDNTFATPYLVRPGTLGADYVIHSATKYLSGHGDVTAGIVATTADRRWELNEI
NKTIGSILGPFEAFLVLRGLKTLPLRMHRQCENASRIACWLAEHPLIERVHYPGLPTHPSHETAARLLPPGLYGAIVSFE
IRHGTRERVTAFLERLRLILPATTLGDVYSEILYPVMASHRGLSPEERQAMGISDGLVRLSVGIEDPEDIIADLEQALAS
LA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=396, Percent_Identity=39.3939393939394, Blast_Score=243, Evalue=3e-64,
Organism=Homo sapiens, GI299473759, Length=396, Percent_Identity=35.3535353535354, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI25453487, Length=395, Percent_Identity=35.1898734177215, Blast_Score=195, Evalue=7e-50,
Organism=Escherichia coli, GI1790375, Length=390, Percent_Identity=38.974358974359, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI1789383, Length=398, Percent_Identity=28.643216080402, Blast_Score=151, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI71980708, Length=378, Percent_Identity=37.037037037037, Blast_Score=248, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI17560056, Length=393, Percent_Identity=38.676844783715, Blast_Score=234, Evalue=6e-62,
Organism=Caenorhabditis elegans, GI17538055, Length=393, Percent_Identity=37.9134860050891, Blast_Score=230, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6319307, Length=397, Percent_Identity=38.2871536523929, Blast_Score=246, Evalue=6e-66,
Organism=Saccharomyces cerevisiae, GI6323333, Length=437, Percent_Identity=32.0366132723112, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6321254, Length=416, Percent_Identity=29.8076923076923, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6321904, Length=409, Percent_Identity=27.8728606356968, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321170, Length=308, Percent_Identity=25.6493506493506, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19922554, Length=391, Percent_Identity=37.5959079283887, Blast_Score=255, Evalue=3e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 43953; Mature: 43953

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDQRRLHFRFATRAVHAGERPNPRDFIPTATPIYPTSTFLYQNIEQMDAALGGAVGVFV
CCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEE
YGRYGNPTTEALETAVASLEEQEAALAFASGMAALHATILSCVEAGDRIVASRDLYGQTI
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHH
TLLRTIFGSLGVRVEFVDILDLAQVEAALRRGGVRLLLCETISNPLLRVTDLPSLAEIAH
HHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHCCEEEEEEHHHCCCCEEECCCCHHHHHHH
RYGARLAVDNTFATPYLVRPGTLGADYVIHSATKYLSGHGDVTAGIVATTADRRWELNEI
HHCCEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHH
NKTIGSILGPFEAFLVLRGLKTLPLRMHRQCENASRIACWLAEHPLIERVHYPGLPTHPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEHHHHCCHHHHHHCCCCCCCCCC
HETAARLLPPGLYGAIVSFEIRHGTRERVTAFLERLRLILPATTLGDVYSEILYPVMASH
HHHHHHHCCCCHHHHHEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
RGLSPEERQAMGISDGLVRLSVGIEDPEDIIADLEQALASLA
CCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDDQRRLHFRFATRAVHAGERPNPRDFIPTATPIYPTSTFLYQNIEQMDAALGGAVGVFV
CCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEE
YGRYGNPTTEALETAVASLEEQEAALAFASGMAALHATILSCVEAGDRIVASRDLYGQTI
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHH
TLLRTIFGSLGVRVEFVDILDLAQVEAALRRGGVRLLLCETISNPLLRVTDLPSLAEIAH
HHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHCCEEEEEEHHHCCCCEEECCCCHHHHHHH
RYGARLAVDNTFATPYLVRPGTLGADYVIHSATKYLSGHGDVTAGIVATTADRRWELNEI
HHCCEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHH
NKTIGSILGPFEAFLVLRGLKTLPLRMHRQCENASRIACWLAEHPLIERVHYPGLPTHPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEHHHHCCHHHHHHCCCCCCCCCC
HETAARLLPPGLYGAIVSFEIRHGTRERVTAFLERLRLILPATTLGDVYSEILYPVMASH
HHHHHHHCCCCHHHHHEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
RGLSPEERQAMGISDGLVRLSVGIEDPEDIIADLEQALASLA
CCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]