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The map label for this gene is frvX [C]

Identifier: 221632302

GI number: 221632302

Start: 277218

End: 278267

Strand: Reverse

Name: frvX [C]

Synonym: trd_0269

Alternate gene names: 221632302

Gene position: 278267-277218 (Counterclockwise)

Preceding gene: 221632310

Following gene: 221632299

Centisome position: 13.89

GC content: 64.76

Gene sequence:

>1050_bases
ATGCGCGAGCGGATCGCCGAGGTACTGACGGAACTGATGGCGCTCCCCGGACCGACTGGTCGGGAGGAGCCAGTTCTGGC
GTGGCTGGAACGTGCTTGGGCTCCGCACGTCAAGCGACTCTGGCGCAGTCGGATCGGCAACCTGCTCGCCTACGTCGGTG
GCGCTGGTCCTCGGCTTCTGCTCACTGCGCATGCCGACGAGTTGAGCTTCGTAGTTCGGTCGATCGATTCGTCCGGCTTG
TTGTGGCTGACGACTGGGCAAGTGCGTGGGGAGCCACAGGAACGGTTCCCAGTCGGCCAACCGGCGCTCGTACTCGGTCG
CGATGTGGCGATCGAGGGGATGTTCGTCCTAGCCACTGGCCATGTCGTGCCCGAAGAGCGGCGGAGCAAGCCGGTGACCT
TCGCTGACTTGTTCGTCGATATCGGTGCCAACTCCAAGCAGGAGGTATTGGAGCGCGGCATCACGGTCGGTTCGAGCGTC
GTCTGGAACCCGCCGACTCGTCGCCTCGGATCGCGCCTCTATGGGAAAGCGATCGATGATCGGGTGGGGCTCGCCCTTTT
GACCCTGCTCGTTCGCCAGATCGACGCGCAGCAGCTTGACTGCGCCCTCTACCTCGCCGCTACCGTGCAGGAAGAAAACG
GACTCCTCGGAGCCAGCTCGCTCCGAGCTGACCTGGACGTCGACTGGGCTATCGCACTCGATGTCGGGCTCGTCGGCGAC
CTGCCGACCATCGGCGAGCAGTGGATGCCGGCAACGCTCGGTGGCGGGCCACAACTCGTGCACAAGGATGCGGCCACCCA
TTACGACCAGCGTTTGCTCTGGCGGCTCGCTGACCTTGCCGATCGCAATGGGTTGCCGGTCCAGCACGTCGTCTTCGATC
GTTACGGCAGTGACGGCGCCGCCCTCATCCGCCAGGGAATCCCGACCGCGCTGGTTGCGGTCGGGGCTCGCCACACGCAT
GCTCCCTTCGAGGCTGTCGATCTCGCTGACGTCGAAGCGACGCTCCACTTGCTCGAGCATGTCGTCTACGAAGGGCCCAC
TGACCAGTGA

Upstream 100 bases:

>100_bases
GCTGCCAGCGCCGGGTGCTACCTGGCCAGATTTCCGCTCCGCCTCGATTCTAAGGTATCCTGGCGCCGAAGGGTCGATCG
AGTGAGCGAGGGGCAGAAGG

Downstream 100 bases:

>100_bases
CGTCCTCCCGGGCTAGGGCACCAGCGCCTCGCCGCCATCTACCCGAATCACATTGCCGGTGATCCACCCGAGCCCCGGTG
CGCTGAGCGCGCGGATCGCT

Product: putative aminopeptidase from family M42

Products: D-glucose; GDP

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MRERIAEVLTELMALPGPTGREEPVLAWLERAWAPHVKRLWRSRIGNLLAYVGGAGPRLLLTAHADELSFVVRSIDSSGL
LWLTTGQVRGEPQERFPVGQPALVLGRDVAIEGMFVLATGHVVPEERRSKPVTFADLFVDIGANSKQEVLERGITVGSSV
VWNPPTRRLGSRLYGKAIDDRVGLALLTLLVRQIDAQQLDCALYLAATVQEENGLLGASSLRADLDVDWAIALDVGLVGD
LPTIGEQWMPATLGGGPQLVHKDAATHYDQRLLWRLADLADRNGLPVQHVVFDRYGSDGAALIRQGIPTALVAVGARHTH
APFEAVDLADVEATLHLLEHVVYEGPTDQ

Sequences:

>Translated_349_residues
MRERIAEVLTELMALPGPTGREEPVLAWLERAWAPHVKRLWRSRIGNLLAYVGGAGPRLLLTAHADELSFVVRSIDSSGL
LWLTTGQVRGEPQERFPVGQPALVLGRDVAIEGMFVLATGHVVPEERRSKPVTFADLFVDIGANSKQEVLERGITVGSSV
VWNPPTRRLGSRLYGKAIDDRVGLALLTLLVRQIDAQQLDCALYLAATVQEENGLLGASSLRADLDVDWAIALDVGLVGD
LPTIGEQWMPATLGGGPQLVHKDAATHYDQRLLWRLADLADRNGLPVQHVVFDRYGSDGAALIRQGIPTALVAVGARHTH
APFEAVDLADVEATLHLLEHVVYEGPTDQ
>Mature_349_residues
MRERIAEVLTELMALPGPTGREEPVLAWLERAWAPHVKRLWRSRIGNLLAYVGGAGPRLLLTAHADELSFVVRSIDSSGL
LWLTTGQVRGEPQERFPVGQPALVLGRDVAIEGMFVLATGHVVPEERRSKPVTFADLFVDIGANSKQEVLERGITVGSSV
VWNPPTRRLGSRLYGKAIDDRVGLALLTLLVRQIDAQQLDCALYLAATVQEENGLLGASSLRADLDVDWAIALDVGLVGD
LPTIGEQWMPATLGGGPQLVHKDAATHYDQRLLWRLADLADRNGLPVQHVVFDRYGSDGAALIRQGIPTALVAVGARHTH
APFEAVDLADVEATLHLLEHVVYEGPTDQ

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=338, Percent_Identity=29.585798816568, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI87082417, Length=351, Percent_Identity=28.4900284900285, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1788727, Length=291, Percent_Identity=30.2405498281787, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: 3.2.1.4

Molecular weight: Translated: 37964; Mature: 37964

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRERIAEVLTELMALPGPTGREEPVLAWLERAWAPHVKRLWRSRIGNLLAYVGGAGPRLL
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEE
LTAHADELSFVVRSIDSSGLLWLTTGQVRGEPQERFPVGQPALVLGRDVAIEGMFVLATG
EEECCHHHHHHHHHCCCCCEEEEEECCCCCCCHHCCCCCCCCEEECCCCCCCCEEEEEEC
HVVPEERRSKPVTFADLFVDIGANSKQEVLERGITVGSSVVWNPPTRRLGSRLYGKAIDD
CCCCHHHCCCCCEEEHHEEECCCCCHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHH
RVGLALLTLLVRQIDAQQLDCALYLAATVQEENGLLGASSLRADLDVDWAIALDVGLVGD
HHHHHHHHHHHHHHCHHHHHHEEEEEEEHHHCCCCEEHHHCCCCCCCCEEEEEECCCCCC
LPTIGEQWMPATLGGGPQLVHKDAATHYDQRLLWRLADLADRNGLPVQHVVFDRYGSDGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHH
ALIRQGIPTALVAVGARHTHAPFEAVDLADVEATLHLLEHVVYEGPTDQ
HHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRERIAEVLTELMALPGPTGREEPVLAWLERAWAPHVKRLWRSRIGNLLAYVGGAGPRLL
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEE
LTAHADELSFVVRSIDSSGLLWLTTGQVRGEPQERFPVGQPALVLGRDVAIEGMFVLATG
EEECCHHHHHHHHHCCCCCEEEEEECCCCCCCHHCCCCCCCCEEECCCCCCCCEEEEEEC
HVVPEERRSKPVTFADLFVDIGANSKQEVLERGITVGSSVVWNPPTRRLGSRLYGKAIDD
CCCCHHHCCCCCEEEHHEEECCCCCHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHH
RVGLALLTLLVRQIDAQQLDCALYLAATVQEENGLLGASSLRADLDVDWAIALDVGLVGD
HHHHHHHHHHHHHHCHHHHHHEEEEEEEHHHCCCCEEHHHCCCCCCCCEEEEEECCCCCC
LPTIGEQWMPATLGGGPQLVHKDAATHYDQRLLWRLADLADRNGLPVQHVVFDRYGSDGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHH
ALIRQGIPTALVAVGARHTHAPFEAVDLADVEATLHLLEHVVYEGPTDQ
HHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPglucose; H2O

Specific reaction: GDP-glucose + H2O = D-glucose + GDP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]