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The map label for this gene is yagI [H]

Identifier: 221632285

GI number: 221632285

Start: 258950

End: 259777

Strand: Reverse

Name: yagI [H]

Synonym: trd_0252

Alternate gene names: 221632285

Gene position: 259777-258950 (Counterclockwise)

Preceding gene: 221632287

Following gene: 221632284

Centisome position: 12.97

GC content: 67.39

Gene sequence:

>828_bases
GTGACGATGCCGGTACAGTCTCCAGCCGTGTGGCGTGCGGTGGCGAGCCTGGACGCCGTGGCGCGAGCCAGCGATGGGTT
GACGCTCAGCGAAGTGGCCCGGGCGATCGGCGAGCCGAAGAGCACGCTGCATGCCGTCCTCGCAACCTTGGTCGAGGCCG
GTCTCCTGATCCGGGAGGAGACCAGCAAACGGTATCGGCTCGGCCCCCACATCCTGGCACTCGCTGGCGCCTACGCTCGT
CAGAGCGACTTGATCCGCGCCTTCACGGAGGTCGCGCGTCCGCTGGCCCGCGAACTCGGCGAGACGATCCAGCTCGCCAT
CCTTCAGGGGCGCGAGGTGCTGTACATCGGCAAGCAGGAAGGCACCCAATGGGTTCGCTTGGCCTCGGAGGTCGGGACGC
GCCTACCCGCGCACGCCACGTCCCTCGGGAAGTGCCTCCTGGCCTGGCTACCAGAGAACGAACTCGGGTGCCTTTTGGCC
GAAGGGCCGCTGGTCGCCCTCACCCCGCGCACCATCACCGATCCGGACGCGCTTCGCGCCGAGCTGGCTCAGGTTCGCGC
GCAGGGATTCGCCATCGATCGCGGTGAGACGCTTCCCGACGTTTGGTGCTTCGGTGCGCCGGTCCGCGACGCGCATGGTG
TCGTCGTGGCCGCTCTGAGCATCTCGGTACCGGTGACCCGTATCGGCGTCGAGCGCGTGGAGGAACTCGTCAGAGCAGCC
CGAGCGGCGGCCGACGAGCTTTCCCGACAACTCGGCTTCAGCGGGGTCGATGATCGAGTGATCGATCGGATGGGAATTCC
TAGTTCGAGGAGGAGGGTTGCACCATGA

Upstream 100 bases:

>100_bases
CACCGAAACGCACACTCCCCTCTTGACAACCATCGCTATTACCCCTAGGATGGAGACAGTTCGGAGAGCGGAACAACTTT
CGAGATTCCGAACGAAAGTG

Downstream 100 bases:

>100_bases
CGAGGAAACTCTCGCGTCGGCAGTTTCTCGGCACCATGCTGGTGGCAGGAGGGAGCGCGCTGCTCGCTGCCTGTCGCCGC
GGTGAGGCGACACCCACCCC

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTMPVQSPAVWRAVASLDAVARASDGLTLSEVARAIGEPKSTLHAVLATLVEAGLLIREETSKRYRLGPHILALAGAYAR
QSDLIRAFTEVARPLARELGETIQLAILQGREVLYIGKQEGTQWVRLASEVGTRLPAHATSLGKCLLAWLPENELGCLLA
EGPLVALTPRTITDPDALRAELAQVRAQGFAIDRGETLPDVWCFGAPVRDAHGVVVAALSISVPVTRIGVERVEELVRAA
RAAADELSRQLGFSGVDDRVIDRMGIPSSRRRVAP

Sequences:

>Translated_275_residues
MTMPVQSPAVWRAVASLDAVARASDGLTLSEVARAIGEPKSTLHAVLATLVEAGLLIREETSKRYRLGPHILALAGAYAR
QSDLIRAFTEVARPLARELGETIQLAILQGREVLYIGKQEGTQWVRLASEVGTRLPAHATSLGKCLLAWLPENELGCLLA
EGPLVALTPRTITDPDALRAELAQVRAQGFAIDRGETLPDVWCFGAPVRDAHGVVVAALSISVPVTRIGVERVEELVRAA
RAAADELSRQLGFSGVDDRVIDRMGIPSSRRRVAP
>Mature_274_residues
TMPVQSPAVWRAVASLDAVARASDGLTLSEVARAIGEPKSTLHAVLATLVEAGLLIREETSKRYRLGPHILALAGAYARQ
SDLIRAFTEVARPLARELGETIQLAILQGREVLYIGKQEGTQWVRLASEVGTRLPAHATSLGKCLLAWLPENELGCLLAE
GPLVALTPRTITDPDALRAELAQVRAQGFAIDRGETLPDVWCFGAPVRDAHGVVVAALSISVPVTRIGVERVEELVRAAR
AAADELSRQLGFSGVDDRVIDRMGIPSSRRRVAP

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=251, Percent_Identity=35.0597609561753, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1788131, Length=250, Percent_Identity=33.2, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI87082359, Length=253, Percent_Identity=30.0395256916996, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1786715, Length=249, Percent_Identity=30.5220883534137, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788581, Length=255, Percent_Identity=31.3725490196078, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789999, Length=258, Percent_Identity=27.1317829457364, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790752, Length=232, Percent_Identity=27.5862068965517, Blast_Score=88, Evalue=8e-19,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 29539; Mature: 29408

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMPVQSPAVWRAVASLDAVARASDGLTLSEVARAIGEPKSTLHAVLATLVEAGLLIREE
CCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
TSKRYRLGPHILALAGAYARQSDLIRAFTEVARPLARELGETIQLAILQGREVLYIGKQE
CCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
GTQWVRLASEVGTRLPAHATSLGKCLLAWLPENELGCLLAEGPLVALTPRTITDPDALRA
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEECCCCCCCHHHHHH
ELAQVRAQGFAIDRGETLPDVWCFGAPVRDAHGVVVAALSISVPVTRIGVERVEELVRAA
HHHHHHHHCCEECCCCCCCCHHHCCCCCCCCCCCEEEEEHHCCCHHHHHHHHHHHHHHHH
RAAADELSRQLGFSGVDDRVIDRMGIPSSRRRVAP
HHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TMPVQSPAVWRAVASLDAVARASDGLTLSEVARAIGEPKSTLHAVLATLVEAGLLIREE
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
TSKRYRLGPHILALAGAYARQSDLIRAFTEVARPLARELGETIQLAILQGREVLYIGKQE
CCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
GTQWVRLASEVGTRLPAHATSLGKCLLAWLPENELGCLLAEGPLVALTPRTITDPDALRA
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEECCCCCCCHHHHHH
ELAQVRAQGFAIDRGETLPDVWCFGAPVRDAHGVVVAALSISVPVTRIGVERVEELVRAA
HHHHHHHHCCEECCCCCCCCHHHCCCCCCCCCCCEEEEEHHCCCHHHHHHHHHHHHHHHH
RAAADELSRQLGFSGVDDRVIDRMGIPSSRRRVAP
HHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]