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The map label for this gene is trmD

Identifier: 221632260

GI number: 221632260

Start: 237215

End: 238021

Strand: Reverse

Name: trmD

Synonym: trd_0226

Alternate gene names: 221632260

Gene position: 238021-237215 (Counterclockwise)

Preceding gene: 221632261

Following gene: 221632259

Centisome position: 11.88

GC content: 63.32

Gene sequence:

>807_bases
ATGCGCTTCGATGTCTTCACGATCTTCCCCGGAATGTTCACCGGTTTCCTGACCGAGAGCATTCTCAAGCGAGCCCAGCA
GGCCGGGTTGATCGAGATCGCCATCCACAACATCCGGGACTGGACGACCGACAAGCACCGCACAGTCGACGACACCCCCT
ACGGCGGCGGACCAGGCATGGTCATGATGGCGCCACCCATCGTTCACGCCGTGGAATCCGTACTCGGACAGGAGCAGAAC
AGCACCCCGATCATCATCATGAGCCCATCCGGCGAACTCTTCACCCAGGACATCGCTCGCCAACTCGCCTGCTTTCCCCG
GCTCGCTCTCATCTGTGGCCGTTACGAGGGAATCGACGATCGCGTGCGCATCATTCTGCGAGCACGCGAACTGTCGATCG
GCGATTATGTGCTCACTGGTGGCGAACTGGCCGCAGCCGTCGTCATCGATGTGGTGAGCCGGCTGGTCCCGGGCGTGATC
GATCCGGAGTCGCTCGCTGAAGAATCGCACAACTCCGGTCTCCTGGAATACCCGCAGTACACGCGTCCACCGATCTTCCG
CGGCTTGGGCGTACCGGAGATTCTGCTGAGCGGCAATCACGCCAAGATCGCCGAGTGGCGGCGGATGATGGCGCTTTGTC
GCACCGCGGCACGCCGCCCGGATCTCTTGGCACGCGCCGCGCTCACGCCGCGCGACCACGAACTCCTGCAACGGTGTCCA
CCGGATCCCTTCGCCCACCAGGGGCCGGTTTACGAGGGAGATCAGCTCGAGCGACCAGAGGGAGGAGAGCAGGGTGCTTC
CCGCTGA

Upstream 100 bases:

>100_bases
TTCGCGAAAAGACCGCCCCCTCCATGAGTGCGCCGGTTTCCTGAGCCGCTGCGCCGGACGCCTCGTTCTGCTAAGCTCTC
CCAAGAGGGAGCAGCCGGCG

Downstream 100 bases:

>100_bases
ACCACGTTCGTTGCTCGGCTGGCTGGTGATCGGCTTCCTCGCTGGGTGGTTGGCCGGACTCCTGACACGCGGGCGCGGAT
TCGGTTGTTTGGGCAATATC

Product: tRNA (guanine-N1)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MRFDVFTIFPGMFTGFLTESILKRAQQAGLIEIAIHNIRDWTTDKHRTVDDTPYGGGPGMVMMAPPIVHAVESVLGQEQN
STPIIIMSPSGELFTQDIARQLACFPRLALICGRYEGIDDRVRIILRARELSIGDYVLTGGELAAAVVIDVVSRLVPGVI
DPESLAEESHNSGLLEYPQYTRPPIFRGLGVPEILLSGNHAKIAEWRRMMALCRTAARRPDLLARAALTPRDHELLQRCP
PDPFAHQGPVYEGDQLERPEGGEQGASR

Sequences:

>Translated_268_residues
MRFDVFTIFPGMFTGFLTESILKRAQQAGLIEIAIHNIRDWTTDKHRTVDDTPYGGGPGMVMMAPPIVHAVESVLGQEQN
STPIIIMSPSGELFTQDIARQLACFPRLALICGRYEGIDDRVRIILRARELSIGDYVLTGGELAAAVVIDVVSRLVPGVI
DPESLAEESHNSGLLEYPQYTRPPIFRGLGVPEILLSGNHAKIAEWRRMMALCRTAARRPDLLARAALTPRDHELLQRCP
PDPFAHQGPVYEGDQLERPEGGEQGASR
>Mature_268_residues
MRFDVFTIFPGMFTGFLTESILKRAQQAGLIEIAIHNIRDWTTDKHRTVDDTPYGGGPGMVMMAPPIVHAVESVLGQEQN
STPIIIMSPSGELFTQDIARQLACFPRLALICGRYEGIDDRVRIILRARELSIGDYVLTGGELAAAVVIDVVSRLVPGVI
DPESLAEESHNSGLLEYPQYTRPPIFRGLGVPEILLSGNHAKIAEWRRMMALCRTAARRPDLLARAALTPRDHELLQRCP
PDPFAHQGPVYEGDQLERPEGGEQGASR

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=237, Percent_Identity=46.4135021097046, Blast_Score=201, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_THERP (B9KXN9)

Other databases:

- EMBL:   CP001275
- RefSeq:   YP_002521481.1
- GeneID:   7355732
- GenomeReviews:   CP001275_GR
- KEGG:   tro:trd_0226
- OMA:   VCGRFEG
- ProtClustDB:   CLSK2807450
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 29602; Mature: 29602

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDVFTIFPGMFTGFLTESILKRAQQAGLIEIAIHNIRDWTTDKHRTVDDTPYGGGPGM
CCCEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCE
VMMAPPIVHAVESVLGQEQNSTPIIIMSPSGELFTQDIARQLACFPRLALICGRYEGIDD
EEECCHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
RVRIILRARELSIGDYVLTGGELAAAVVIDVVSRLVPGVIDPESLAEESHNSGLLEYPQY
HHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCC
TRPPIFRGLGVPEILLSGNHAKIAEWRRMMALCRTAARRPDLLARAALTPRDHELLQRCP
CCCCHHHCCCCCHHEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHCC
PDPFAHQGPVYEGDQLERPEGGEQGASR
CCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRFDVFTIFPGMFTGFLTESILKRAQQAGLIEIAIHNIRDWTTDKHRTVDDTPYGGGPGM
CCCEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCE
VMMAPPIVHAVESVLGQEQNSTPIIIMSPSGELFTQDIARQLACFPRLALICGRYEGIDD
EEECCHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
RVRIILRARELSIGDYVLTGGELAAAVVIDVVSRLVPGVIDPESLAEESHNSGLLEYPQY
HHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCC
TRPPIFRGLGVPEILLSGNHAKIAEWRRMMALCRTAARRPDLLARAALTPRDHELLQRCP
CCCCHHHCCCCCHHEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHCC
PDPFAHQGPVYEGDQLERPEGGEQGASR
CCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA